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The map label for this gene is truA [H]

Identifier: 160875946

GI number: 160875946

Start: 3375478

End: 3376263

Strand: Reverse

Name: truA [H]

Synonym: Sbal195_2836

Alternate gene names: 160875946

Gene position: 3376263-3375478 (Counterclockwise)

Preceding gene: 160875947

Following gene: 160875945

Centisome position: 63.14

GC content: 48.98

Gene sequence:

>786_bases
ATGCGGATTGCATTAGGTATTGAATATGACGGCAATGGTTATTTTGGCTGGCAACGTCAGGCCGAAGTCGATTCAGTACA
AGCGCAATTAGAACGGGCGTTATCGATAGTGGCCAACGAACCTATCGGCGTTTTCTGCGCTGGCCGTACCGATGCGGGCG
TGCATGCCACGGGCCAAGTGGTTCATTTTGAAACCAATGCCATTCGTAATGAAGGCGCATGGACCTTAGGGGTGAATGCC
AATTTGCCCGATAATATTGCCGTGCGTTGGGTTAAAGAAGTCGACGACAGTTTTCACGCGCGCTTTTCGGCTACCGCCAG
ACGCTACCGTTATGTGATTTATAATCACAGTTTTCGCCCTGGAATTTTACGCCATGGTGTCAGCCATTATCACGGCGATA
TTGATGCAGACAGAATGCACCAAGCGGCGCAGGCACTCTTAGGTGAGCAAGATTTTACCAGCTTTCGCGCAGTCCAGTGC
CAGTCAAAAACGCCGTTTCGTAATGTACATTGCGTCAATGTTACGCGCCAAGGCATGTATGTGATTGTTGATATCGCCGC
CAACGCCTTTTTACACCACATGGTGCGCAATATCGTCGGTTCATTATTAGAAATCGGTTTAGGCAATCAGCCGCTGACGT
GGATGGGTGATTTGCTCGCGTTAAAAGACAGAAACCAAGCCGCAGCGACGGCTAAACCCCATGGGCTCTATTTAGTTGAT
GTGACTTATCCAGAACAATATCAATTGCCTAAGCTCGCCCTTGGGCCATTATTCATGTTGGATTGA

Upstream 100 bases:

>100_bases
CCGATTGAGAGAGTTTCAACCTAACGGCGCCTTCAGCGCCGTTTTTTTTATGGGCTGCTGTGATAAAATGCGCCGCTAAA
TCAATTGTGAGGAAAAGTGG

Downstream 100 bases:

>100_bases
AAAGATCTTATTTGAGAGATTATCGAATACGAGGATGTACGGACATGCTAACAGCGCCAGCGTCAAATGCAGCATTGGAA
CAGTGGTTGGATTATTTGTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRIALGIEYDGNGYFGWQRQAEVDSVQAQLERALSIVANEPIGVFCAGRTDAGVHATGQVVHFETNAIRNEGAWTLGVNA
NLPDNIAVRWVKEVDDSFHARFSATARRYRYVIYNHSFRPGILRHGVSHYHGDIDADRMHQAAQALLGEQDFTSFRAVQC
QSKTPFRNVHCVNVTRQGMYVIVDIAANAFLHHMVRNIVGSLLEIGLGNQPLTWMGDLLALKDRNQAAATAKPHGLYLVD
VTYPEQYQLPKLALGPLFMLD

Sequences:

>Translated_261_residues
MRIALGIEYDGNGYFGWQRQAEVDSVQAQLERALSIVANEPIGVFCAGRTDAGVHATGQVVHFETNAIRNEGAWTLGVNA
NLPDNIAVRWVKEVDDSFHARFSATARRYRYVIYNHSFRPGILRHGVSHYHGDIDADRMHQAAQALLGEQDFTSFRAVQC
QSKTPFRNVHCVNVTRQGMYVIVDIAANAFLHHMVRNIVGSLLEIGLGNQPLTWMGDLLALKDRNQAAATAKPHGLYLVD
VTYPEQYQLPKLALGPLFMLD
>Mature_261_residues
MRIALGIEYDGNGYFGWQRQAEVDSVQAQLERALSIVANEPIGVFCAGRTDAGVHATGQVVHFETNAIRNEGAWTLGVNA
NLPDNIAVRWVKEVDDSFHARFSATARRYRYVIYNHSFRPGILRHGVSHYHGDIDADRMHQAAQALLGEQDFTSFRAVQC
QSKTPFRNVHCVNVTRQGMYVIVDIAANAFLHHMVRNIVGSLLEIGLGNQPLTWMGDLLALKDRNQAAATAKPHGLYLVD
VTYPEQYQLPKLALGPLFMLD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=261, Percent_Identity=26.8199233716475, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=71.9230769230769, Blast_Score=399, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29128; Mature: 29128

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALGIEYDGNGYFGWQRQAEVDSVQAQLERALSIVANEPIGVFCAGRTDAGVHATGQV
CEEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCEE
VHFETNAIRNEGAWTLGVNANLPDNIAVRWVKEVDDSFHARFSATARRYRYVIYNHSFRP
EEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHEEEEEEEECCCCC
GILRHGVSHYHGDIDADRMHQAAQALLGEQDFTSFRAVQCQSKTPFRNVHCVNVTRQGMY
HHHHHCHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCEE
VIVDIAANAFLHHMVRNIVGSLLEIGLGNQPLTWMGDLLALKDRNQAAATAKPHGLYLVD
EEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCCCCCCEEEEE
VTYPEQYQLPKLALGPLFMLD
ECCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MRIALGIEYDGNGYFGWQRQAEVDSVQAQLERALSIVANEPIGVFCAGRTDAGVHATGQV
CEEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCEE
VHFETNAIRNEGAWTLGVNANLPDNIAVRWVKEVDDSFHARFSATARRYRYVIYNHSFRP
EEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHEEEEEEEECCCCC
GILRHGVSHYHGDIDADRMHQAAQALLGEQDFTSFRAVQCQSKTPFRNVHCVNVTRQGMY
HHHHHCHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCEE
VIVDIAANAFLHHMVRNIVGSLLEIGLGNQPLTWMGDLLALKDRNQAAATAKPHGLYLVD
EEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCCCCCCEEEEE
VTYPEQYQLPKLALGPLFMLD
ECCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA