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The map label for this gene is trpCF [H]

Identifier: 160875915

GI number: 160875915

Start: 3337686

End: 3339173

Strand: Direct

Name: trpCF [H]

Synonym: Sbal195_2804

Alternate gene names: 160875915

Gene position: 3337686-3339173 (Clockwise)

Preceding gene: 160875914

Following gene: 160875916

Centisome position: 62.42

GC content: 51.48

Gene sequence:

>1488_bases
ATGTCTCAAGTAGAAGTGACTCCAACAGCAATGACTCAAGACCTGCGTACTCAGCCAGAGGCAGCCACAAACGCTATGCC
AAAAAGTAATGTGTTAACCCGTATTGTCGATACCAAAGCGGCCCATATTGCCGCCCTGAAGCTGCGTTTCCCTGAGGCGA
GTCTGTCGCCAAAAATCTCTGACCGCAGTTTATTTGCCGCACTCAAGGCACCCAAGGCGGGTTACATTTTAGAATGCAAA
AAAGCCAGTCCATCCAAAGGGCTTATCCGCGATGTGTTTGATGTAGAAGCCATCGCCGATATTTACGGTAAATATGCGGC
GGGCATTTCGGTATTAACCGACGAGCAATTTTTCCAAGGGGATATGGACTATATCCCTAAGGTGCGCGCTCGGGTCAACC
AACCGATTCTATGCAAAGATTTTTTTGTGGACGAGTACCAAATCAAACTCGCTGCTCACCAAGGCGCCGATGCGATTCTG
TTGATGCTGTCCGTGCTTGATGATGAACAATATCAACATCTAGCGAAAGAAGCGGCTAAGTATCAACTCGATGTACTGAC
CGAAGTCAGTAATGAAGAAGAACTTAAGCGCGCCATCGCATTAGATGCGCCGATTATTGGCATCAACAACCGTAACTTAA
GGGATTTGAGTACCGATCTCGCTACCACAGAGACCTTAGCGCCACACATCGGCAGCGACCGCGTGGTGATCAGCGAATCT
GGCATCTACAACAATGCGCAAGTGCGCCGCCTGAGTCCGCTGGTCGATGGCTTCCTCGTTGGCAGCTCGATTATGGCCGA
GGCTGATATCGACTTAGCCTGCCGTAAATTGATCTTCGGTCACAATAAAGTCTGCGGCCTCACCCGTGTGGAAGATATGC
GCGCCGCGGCAACAGCAGGCGCTGTCTATGGTGGGCTGATTTTTGCTGAAAAATCCCCGCGCGCGTTAACCCTTGATGCC
GCAGAACAACTGCTCAGGGCGTATCGCGCATCCGATGCCCCCGCCATTGAATTTGTCGGCGTGTTTGTTAATGCCGCGGC
AAGCACCATCACCGATATCGCCGCCCGTTTGCAGTTATCTGCCGTACAGCTGCACGGCACAGAAACTGAACTTGAAATAG
CACAGCTCGCCGAGCGACTTGAGCAAGCGGGACTCACCACCCAAATTTGGAAAGCCGTCAGCGTCGATGCGCAAACGGGT
GAACTTGGCAACTTGCCTCTGGGAGCACAGCGTTATCTGTTTGATAGTAAAACGGCAGGCCAATTTGGCGGCTCGGGCCA
AGCCTTTAATTGGCAAAATATTGATGTGAAGTCCTTAGGCCAGCAAAAGGCCCATGCCATGTTGGCGGGCGGACTCAACG
CCGACAATGCGGCCAGTGCCAATGCCCAAGGATTTTACGGTTTAGATTTTAATTCTGGCCTAGAAACCGCCGCAGGAATT
AAATCGGCGCAACTGATCCAAACGGCTTTTACCCATTTACGTCTCTAG

Upstream 100 bases:

>100_bases
GCTACCATTCAAAGCGGCAAAGCATTTGAGTTATTAAGCCAGCTCGCAAAAGTCAGCGGTGAAGCCCATGTCAACGGTCA
AGAAAGAGGAAGATAAGCGA

Downstream 100 bases:

>100_bases
CCTATCGCCTAAGCAAGAAGCCCTAGATGCAGTTGGGCTCGACAAGCGTGATGCTCGACAAAAAATATGAACCTATTAAT
GCCAGTGGCCAAATAGATAA

Product: bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: Indole-3-glycerol phosphate synthase; IGPS; N-(5'-phospho-ribosyl)anthranilate isomerase; PRAI [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSQVEVTPTAMTQDLRTQPEAATNAMPKSNVLTRIVDTKAAHIAALKLRFPEASLSPKISDRSLFAALKAPKAGYILECK
KASPSKGLIRDVFDVEAIADIYGKYAAGISVLTDEQFFQGDMDYIPKVRARVNQPILCKDFFVDEYQIKLAAHQGADAIL
LMLSVLDDEQYQHLAKEAAKYQLDVLTEVSNEEELKRAIALDAPIIGINNRNLRDLSTDLATTETLAPHIGSDRVVISES
GIYNNAQVRRLSPLVDGFLVGSSIMAEADIDLACRKLIFGHNKVCGLTRVEDMRAAATAGAVYGGLIFAEKSPRALTLDA
AEQLLRAYRASDAPAIEFVGVFVNAAASTITDIAARLQLSAVQLHGTETELEIAQLAERLEQAGLTTQIWKAVSVDAQTG
ELGNLPLGAQRYLFDSKTAGQFGGSGQAFNWQNIDVKSLGQQKAHAMLAGGLNADNAASANAQGFYGLDFNSGLETAAGI
KSAQLIQTAFTHLRL

Sequences:

>Translated_495_residues
MSQVEVTPTAMTQDLRTQPEAATNAMPKSNVLTRIVDTKAAHIAALKLRFPEASLSPKISDRSLFAALKAPKAGYILECK
KASPSKGLIRDVFDVEAIADIYGKYAAGISVLTDEQFFQGDMDYIPKVRARVNQPILCKDFFVDEYQIKLAAHQGADAIL
LMLSVLDDEQYQHLAKEAAKYQLDVLTEVSNEEELKRAIALDAPIIGINNRNLRDLSTDLATTETLAPHIGSDRVVISES
GIYNNAQVRRLSPLVDGFLVGSSIMAEADIDLACRKLIFGHNKVCGLTRVEDMRAAATAGAVYGGLIFAEKSPRALTLDA
AEQLLRAYRASDAPAIEFVGVFVNAAASTITDIAARLQLSAVQLHGTETELEIAQLAERLEQAGLTTQIWKAVSVDAQTG
ELGNLPLGAQRYLFDSKTAGQFGGSGQAFNWQNIDVKSLGQQKAHAMLAGGLNADNAASANAQGFYGLDFNSGLETAAGI
KSAQLIQTAFTHLRL
>Mature_494_residues
SQVEVTPTAMTQDLRTQPEAATNAMPKSNVLTRIVDTKAAHIAALKLRFPEASLSPKISDRSLFAALKAPKAGYILECKK
ASPSKGLIRDVFDVEAIADIYGKYAAGISVLTDEQFFQGDMDYIPKVRARVNQPILCKDFFVDEYQIKLAAHQGADAILL
MLSVLDDEQYQHLAKEAAKYQLDVLTEVSNEEELKRAIALDAPIIGINNRNLRDLSTDLATTETLAPHIGSDRVVISESG
IYNNAQVRRLSPLVDGFLVGSSIMAEADIDLACRKLIFGHNKVCGLTRVEDMRAAATAGAVYGGLIFAEKSPRALTLDAA
EQLLRAYRASDAPAIEFVGVFVNAAASTITDIAARLQLSAVQLHGTETELEIAQLAERLEQAGLTTQIWKAVSVDAQTGE
LGNLPLGAQRYLFDSKTAGQFGGSGQAFNWQNIDVKSLGQQKAHAMLAGGLNADNAASANAQGFYGLDFNSGLETAAGIK
SAQLIQTAFTHLRL

Specific function: Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the trpF domain; the second reaction is catalyzed by the synthase, coded by the trpC domain [H]

COG id: COG0134

COG function: function code E; Indole-3-glycerol phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=470, Percent_Identity=50, Blast_Score=407, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6322638, Length=234, Percent_Identity=32.9059829059829, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013798
- InterPro:   IPR001468
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00218 IGPS; PF00697 PRAI [H]

EC number: =4.1.1.48; =5.3.1.24 [H]

Molecular weight: Translated: 53283; Mature: 53152

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00614 IGPS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVEVTPTAMTQDLRTQPEAATNAMPKSNVLTRIVDTKAAHIAALKLRFPEASLSPKIS
CCCEECCHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCC
DRSLFAALKAPKAGYILECKKASPSKGLIRDVFDVEAIADIYGKYAAGISVLTDEQFFQG
CHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCC
DMDYIPKVRARVNQPILCKDFFVDEYQIKLAAHQGADAILLMLSVLDDEQYQHLAKEAAK
CHHHHHHHHHHCCCCEEEHHHCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHH
YQLDVLTEVSNEEELKRAIALDAPIIGINNRNLRDLSTDLATTETLAPHIGSDRVVISES
HHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
GIYNNAQVRRLSPLVDGFLVGSSIMAEADIDLACRKLIFGHNKVCGLTRVEDMRAAATAG
CCCCCCHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AVYGGLIFAEKSPRALTLDAAEQLLRAYRASDAPAIEFVGVFVNAAASTITDIAARLQLS
HHHHCEEEECCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
AVQLHGTETELEIAQLAERLEQAGLTTQIWKAVSVDAQTGELGNLPLGAQRYLFDSKTAG
EEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCC
QFGGSGQAFNWQNIDVKSLGQQKAHAMLAGGLNADNAASANAQGFYGLDFNSGLETAAGI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCHHHCC
KSAQLIQTAFTHLRL
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQVEVTPTAMTQDLRTQPEAATNAMPKSNVLTRIVDTKAAHIAALKLRFPEASLSPKIS
CCEECCHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCC
DRSLFAALKAPKAGYILECKKASPSKGLIRDVFDVEAIADIYGKYAAGISVLTDEQFFQG
CHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCC
DMDYIPKVRARVNQPILCKDFFVDEYQIKLAAHQGADAILLMLSVLDDEQYQHLAKEAAK
CHHHHHHHHHHCCCCEEEHHHCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHH
YQLDVLTEVSNEEELKRAIALDAPIIGINNRNLRDLSTDLATTETLAPHIGSDRVVISES
HHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
GIYNNAQVRRLSPLVDGFLVGSSIMAEADIDLACRKLIFGHNKVCGLTRVEDMRAAATAG
CCCCCCHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AVYGGLIFAEKSPRALTLDAAEQLLRAYRASDAPAIEFVGVFVNAAASTITDIAARLQLS
HHHHCEEEECCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
AVQLHGTETELEIAQLAERLEQAGLTTQIWKAVSVDAQTGELGNLPLGAQRYLFDSKTAG
EEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCC
QFGGSGQAFNWQNIDVKSLGQQKAHAMLAGGLNADNAASANAQGFYGLDFNSGLETAAGI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCHHHCC
KSAQLIQTAFTHLRL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]