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The map label for this gene is pepE

Identifier: 160875785

GI number: 160875785

Start: 3170256

End: 3170975

Strand: Direct

Name: pepE

Synonym: Sbal195_2673

Alternate gene names: 160875785

Gene position: 3170256-3170975 (Clockwise)

Preceding gene: 160875783

Following gene: 160875786

Centisome position: 59.29

GC content: 48.75

Gene sequence:

>720_bases
ATGACAATCAATGCCCTACTACTGAGCAGCTCTCGTGTCGGTGATACACCTTATCTTGCCCACGCGATCCCTTTTATTAA
ACCGCTTACCACCAACGCGCGAAAATGGATTTTTATCCCCTATGCTGGCGTCAGCATGAGTTATGACACTTATTTAGCGT
CTGTAGTGACAGGATTAAGTGGGCTTGAACTCGATATCAGTGGCATTCATCAGCATCCCGATCCTCAACAAGCGATAAAA
GATGCCGACGGGATTTTAATCGGCGGCGGTAACACCTTTCATTTATTGCACCAACTATATCGCTATGATCTGGTTACGCT
GATTGGTGAGCAAGTCGCCCTTGGTAAACCTTATATCGGCTGGAGTGCGGGTTCTAACGTATCGGGACTGAGCATTCGCA
CCACGAACGATATGCCCATCATCGAGCCGCCGTCGTTCAATGCATTAAACTTAGTGCCGTTCCAGCTAAATCCGCATTAT
TCCAATTACCAAGCACCGGGCCACAACGGTGAAACCCGTGCCCAGCGGTTATTAGAGTTCACTAAAGTCGACCCATTGAC
TCCAGTGGTCGGCATAGTTGAAGGCTCAGCCCTGTGGCGTCAAGGAGACAAACTCTCACTCTTAGGTAACCAACCTGCGT
ATTTGTTCTGCGGAGAACAGCAAGAAATCCCTATCCCTGTGGGCAGCGATCTCTCAAACTTACTCAAACCATCGCTCTGA

Upstream 100 bases:

>100_bases
GGCTATCGGCGCTACAGCGCAATTCTGCTTTATTTCTCTATGTTTATCGGGATTAATCTTTGTTAACATGCCTGCACACT
CCGAGATTTCAGGATACAAA

Downstream 100 bases:

>100_bases
AAGGCATGTTCAGAGTCACTTTTTTGAGGCTCACAAAATAATGTGAGCCTCAACCACTAATATTGTTTTCTTTAAGTCAT
CGTTTGAACTAAATCGGTTG

Product: peptidase E

Products: NA

Alternate protein names: Alpha-aspartyl dipeptidase; Asp-specific dipeptidase; Dipeptidase E

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTINALLLSSSRVGDTPYLAHAIPFIKPLTTNARKWIFIPYAGVSMSYDTYLASVVTGLSGLELDISGIHQHPDPQQAIK
DADGILIGGGNTFHLLHQLYRYDLVTLIGEQVALGKPYIGWSAGSNVSGLSIRTTNDMPIIEPPSFNALNLVPFQLNPHY
SNYQAPGHNGETRAQRLLEFTKVDPLTPVVGIVEGSALWRQGDKLSLLGNQPAYLFCGEQQEIPIPVGSDLSNLLKPSL

Sequences:

>Translated_239_residues
MTINALLLSSSRVGDTPYLAHAIPFIKPLTTNARKWIFIPYAGVSMSYDTYLASVVTGLSGLELDISGIHQHPDPQQAIK
DADGILIGGGNTFHLLHQLYRYDLVTLIGEQVALGKPYIGWSAGSNVSGLSIRTTNDMPIIEPPSFNALNLVPFQLNPHY
SNYQAPGHNGETRAQRLLEFTKVDPLTPVVGIVEGSALWRQGDKLSLLGNQPAYLFCGEQQEIPIPVGSDLSNLLKPSL
>Mature_238_residues
TINALLLSSSRVGDTPYLAHAIPFIKPLTTNARKWIFIPYAGVSMSYDTYLASVVTGLSGLELDISGIHQHPDPQQAIKD
ADGILIGGGNTFHLLHQLYRYDLVTLIGEQVALGKPYIGWSAGSNVSGLSIRTTNDMPIIEPPSFNALNLVPFQLNPHYS
NYQAPGHNGETRAQRLLEFTKVDPLTPVVGIVEGSALWRQGDKLSLLGNQPAYLFCGEQQEIPIPVGSDLSNLLKPSL

Specific function: Hydrolyzes dipeptides containing N-terminal aspartate residues. May play a role in allowing the cell to use peptide aspartate to spare carbon otherwise required for the synthesis of the aspartate family of amino acids

COG id: COG3340

COG function: function code E; Peptidase E

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S51 family

Homologues:

Organism=Escherichia coli, GI1790452, Length=223, Percent_Identity=41.2556053811659, Blast_Score=182, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24641669, Length=223, Percent_Identity=43.4977578475336, Blast_Score=162, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPE_SHEB9 (A9L579)

Other databases:

- EMBL:   CP000891
- RefSeq:   YP_001555101.1
- ProteinModelPortal:   A9L579
- SMR:   A9L579
- MEROPS:   S51.001
- GeneID:   5754433
- GenomeReviews:   CP000891_GR
- KEGG:   sbn:Sbal195_2673
- HOGENOM:   HBG298965
- OMA:   ALWRQGD
- ProtClustDB:   PRK05282
- BioCyc:   SBAL399599:SBAL195_2673-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00510
- InterPro:   IPR005320
- InterPro:   IPR023172

Pfam domain/function: PF03575 Peptidase_S51

EC number: =3.4.13.21

Molecular weight: Translated: 25974; Mature: 25843

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: ACT_SITE 122-122 ACT_SITE 137-137 ACT_SITE 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINALLLSSSRVGDTPYLAHAIPFIKPLTTNARKWIFIPYAGVSMSYDTYLASVVTGLS
CEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCC
GLELDISGIHQHPDPQQAIKDADGILIGGGNTFHLLHQLYRYDLVTLIGEQVALGKPYIG
CCEEEECCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WSAGSNVSGLSIRTTNDMPIIEPPSFNALNLVPFQLNPHYSNYQAPGHNGETRAQRLLEF
CCCCCCCCCEEEEECCCCCEECCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
TKVDPLTPVVGIVEGSALWRQGDKLSLLGNQPAYLFCGEQQEIPIPVGSDLSNLLKPSL
HCCCCCCCEEEEECCCHHHCCCCEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TINALLLSSSRVGDTPYLAHAIPFIKPLTTNARKWIFIPYAGVSMSYDTYLASVVTGLS
EEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCC
GLELDISGIHQHPDPQQAIKDADGILIGGGNTFHLLHQLYRYDLVTLIGEQVALGKPYIG
CCEEEECCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WSAGSNVSGLSIRTTNDMPIIEPPSFNALNLVPFQLNPHYSNYQAPGHNGETRAQRLLEF
CCCCCCCCCEEEEECCCCCEECCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
TKVDPLTPVVGIVEGSALWRQGDKLSLLGNQPAYLFCGEQQEIPIPVGSDLSNLLKPSL
HCCCCCCCEEEEECCCHHHCCCCEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA