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The map label for this gene is iciA [H]

Identifier: 160875783

GI number: 160875783

Start: 3168076

End: 3169011

Strand: Direct

Name: iciA [H]

Synonym: Sbal195_2671

Alternate gene names: 160875783

Gene position: 3168076-3169011 (Clockwise)

Preceding gene: 160875781

Following gene: 160875785

Centisome position: 59.25

GC content: 50.75

Gene sequence:

>936_bases
ATGCTGGAATACGCCAATCTCAAAGCCCTTGCTGTTGTGGTCTCGGAAGGCGGGTTCGATCGCGCCGCCAAAGTGCTGCA
CATCACCCAGTCGGCAGTATCGCAACGTATTCGGCAACTTGAGGAAAAAGTTGGTCAGTCATTACTTATTCGCTCCAATC
CCGTGGTCGCGACACCCACAGGCAAACGCCTACTGCGCCACTATTCGCAGGTGCAATTACTAGAGAGTGAATTAAGAGCT
GAGATGGACGCCGACGATCCCAACCATCCGACCACAGTGCGTATTGCTGTGAATGCCGATAGCTTAGCCACGTGGTTTTT
ACCCGCGCTGGCTGGCATGTTCAGTCGCCACGCTTGGTTATTGGAATTAATTGTCGATGATGAGTCTTACACCCATCATC
TATTGAAAAATGGTGAAGCAGTCGGCTGTGTCACCACAACTGAGGTTCCGATGGCGGGCTGCAGCAGTGAGTTTTTAGGC
ACCATGGAATATCTATGTGTGGCCACGCCCGAATTTGCTGCACGCTATTTTAGCGAGCCGAGCGACACTCAGTCACTTAG
CAACACTCAGCTGTTTAACGGCATTAGCCAAGCCCAGTTAGCGAAGGCGCCAGCAGTGGTGTTCTCCACCAAAGATAAGC
TGCACGAGAAGTTTCTCGCCCAATATTTCAATATGACGCCAGGGCAATGGTGGCAACATACAATCCCCTCTTCAGAAAGT
TTTTTAGAAGCGATTAACTTAAGCTTAGGTTATGGACTCGTCGGTCATCTGCAAGCTAAGCCATTAATCGATCAAGGAAT
ACTGATTGAGCTCACGCCAGAGCGGCGCATGCGCGTGCCACTGTTTTGGCAGCATTGGAATATTAAGGCGAAGCAAACGA
CACTGGTCTATCGCGCCCTTGCGGCAACCGCGCAACACAATTTACTGCAGGCATAA

Upstream 100 bases:

>100_bases
CGCTAGCATAGTCAAAACAACAAAATTAATATAACTAATGTTTTTTATGTATCATAAGTAAAATTAATAATCATCAAACA
CACATCAATTAAGGATGACT

Downstream 100 bases:

>100_bases
AAAATCGGGCTCAAACCAAAGTTTGAGCCCGATTTTGAGAGTCATAGATCTAATTTCAAGTGAGTCTTAAAACTTAAACC
TAACTCACTCTTTATTAGCT

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MLEYANLKALAVVVSEGGFDRAAKVLHITQSAVSQRIRQLEEKVGQSLLIRSNPVVATPTGKRLLRHYSQVQLLESELRA
EMDADDPNHPTTVRIAVNADSLATWFLPALAGMFSRHAWLLELIVDDESYTHHLLKNGEAVGCVTTTEVPMAGCSSEFLG
TMEYLCVATPEFAARYFSEPSDTQSLSNTQLFNGISQAQLAKAPAVVFSTKDKLHEKFLAQYFNMTPGQWWQHTIPSSES
FLEAINLSLGYGLVGHLQAKPLIDQGILIELTPERRMRVPLFWQHWNIKAKQTTLVYRALAATAQHNLLQA

Sequences:

>Translated_311_residues
MLEYANLKALAVVVSEGGFDRAAKVLHITQSAVSQRIRQLEEKVGQSLLIRSNPVVATPTGKRLLRHYSQVQLLESELRA
EMDADDPNHPTTVRIAVNADSLATWFLPALAGMFSRHAWLLELIVDDESYTHHLLKNGEAVGCVTTTEVPMAGCSSEFLG
TMEYLCVATPEFAARYFSEPSDTQSLSNTQLFNGISQAQLAKAPAVVFSTKDKLHEKFLAQYFNMTPGQWWQHTIPSSES
FLEAINLSLGYGLVGHLQAKPLIDQGILIELTPERRMRVPLFWQHWNIKAKQTTLVYRALAATAQHNLLQA
>Mature_311_residues
MLEYANLKALAVVVSEGGFDRAAKVLHITQSAVSQRIRQLEEKVGQSLLIRSNPVVATPTGKRLLRHYSQVQLLESELRA
EMDADDPNHPTTVRIAVNADSLATWFLPALAGMFSRHAWLLELIVDDESYTHHLLKNGEAVGCVTTTEVPMAGCSSEFLG
TMEYLCVATPEFAARYFSEPSDTQSLSNTQLFNGISQAQLAKAPAVVFSTKDKLHEKFLAQYFNMTPGQWWQHTIPSSES
FLEAINLSLGYGLVGHLQAKPLIDQGILIELTPERRMRVPLFWQHWNIKAKQTTLVYRALAATAQHNLLQA

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=301, Percent_Identity=39.202657807309, Blast_Score=194, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34657; Mature: 34657

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEYANLKALAVVVSEGGFDRAAKVLHITQSAVSQRIRQLEEKVGQSLLIRSNPVVATPT
CCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEECCC
GKRLLRHYSQVQLLESELRAEMDADDPNHPTTVRIAVNADSLATWFLPALAGMFSRHAWL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHEE
LELIVDDESYTHHLLKNGEAVGCVTTTEVPMAGCSSEFLGTMEYLCVATPEFAARYFSEP
EEEEECCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCC
SDTQSLSNTQLFNGISQAQLAKAPAVVFSTKDKLHEKFLAQYFNMTPGQWWQHTIPSSES
CCHHHHCHHHHHCCHHHHHHHHCCEEEEECHHHHHHHHHHHHHCCCCCCHHHCCCCCHHH
FLEAINLSLGYGLVGHLQAKPLIDQGILIELTPERRMRVPLFWQHWNIKAKQTTLVYRAL
HHHHHHHHHCCCHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHH
AATAQHNLLQA
HHHHHHHHCCC
>Mature Secondary Structure
MLEYANLKALAVVVSEGGFDRAAKVLHITQSAVSQRIRQLEEKVGQSLLIRSNPVVATPT
CCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEECCC
GKRLLRHYSQVQLLESELRAEMDADDPNHPTTVRIAVNADSLATWFLPALAGMFSRHAWL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHEE
LELIVDDESYTHHLLKNGEAVGCVTTTEVPMAGCSSEFLGTMEYLCVATPEFAARYFSEP
EEEEECCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCC
SDTQSLSNTQLFNGISQAQLAKAPAVVFSTKDKLHEKFLAQYFNMTPGQWWQHTIPSSES
CCHHHHCHHHHHCCHHHHHHHHCCEEEEECHHHHHHHHHHHHHCCCCCCHHHCCCCCHHH
FLEAINLSLGYGLVGHLQAKPLIDQGILIELTPERRMRVPLFWQHWNIKAKQTTLVYRAL
HHHHHHHHHCCCHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHH
AATAQHNLLQA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9286976 [H]