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The map label for this gene is holB [H]

Identifier: 160875683

GI number: 160875683

Start: 3045270

End: 3046226

Strand: Reverse

Name: holB [H]

Synonym: Sbal195_2571

Alternate gene names: 160875683

Gene position: 3046226-3045270 (Counterclockwise)

Preceding gene: 160875684

Following gene: 160875682

Centisome position: 56.97

GC content: 48.07

Gene sequence:

>957_bases
ATGGTAGCCGAGTCAGGGTTTACAGCTGCGCAATTGCCTTGGCTCGAAGGGCCAAGACAGGCATTTTTAGCGCAACTCAC
GCGGCAAAAAATGCCCCATGCACAGCTTGTCGGCATTGATTCTGCCTATGGTGGTGAGCTGCTGAGTGTGTTCATGGCGC
GTGCGGCCATGTGTTTGCAGCCAACTTCAGCGGGGGCCTGCGGTTTTTGTAAGTCTTGTCAGCTTTTTAATGCGGGTAAT
CATCCCGATTTTTACCATATTGCCGCCGATGGCAATCAAATTAAAGTCGATCAAATTCGTGAGCTTTGCACACGTCTGAC
GGCGACTGCCCAGCAAAGCGGTATGCGAGTGGCGATTATTCATCATAGCGAGCGGCTTAACTCCGCTGCCGCCAACGCAC
TCCTTAAAACCCTTGAAGAGCCTGGTAAAGACACTTTACTGATATTGCAGTCGGATACGCCTGCACGTCTAATGGCGACG
GTCAGCAGCCGCTGCCAACGCATTCCCTTCGTTGTGCCAAGTCGAGAGATCATCAAAAACTGGTTAGCGCAGCAATGTGA
TATCCAAGAGGATGTCACTTGGTGCTTGAGCGTGGTGGGTGGACCGCTAAAGTTGGCAGAGAGCTTGCAATCTGAAGGTG
GTCAACAGAGCCGTTATCAATCCTTGTTACAGTTTCGCAAAGATTGGGCCCAAAGTTTGTCGTCTGGTCATCTGTGTGCT
AGTTTGTTAACTATAAGTGAACAACAAATAATTGATGTTCTTAAGGTTTTATACCTGCTTATACGACAGGTGTTACTGAA
AAATAGCCACCAAGATGCGTTTATCCATGCGCAGCTCGGGAACTTGGCGGCCAAGATCATGGGAATGTGTCATAGCTTAA
GTACAATGCCGAGCGTCAATTATCTGGCCTTGTGTCAAAGTTATGTCTTAGAATATAGAGAGATAACAAATAAGTAA

Upstream 100 bases:

>100_bases
TTAAACTTGCCAGCGAAGATGACAGCATAGTGGTTATCGATGCGAGTCAAACCATGGCCGAAGTGCATAAAGATATCTTA
GCCGTACTACAGGCGATGGC

Downstream 100 bases:

>100_bases
TATGCCATGGTAGATCTCGTCGTTAATTTTGATACCTTACATCAGCTATATCGTGCCTATATGCCTTTTATTAAGCCGGC
AGGATTATTCATAGCCACCA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MVAESGFTAAQLPWLEGPRQAFLAQLTRQKMPHAQLVGIDSAYGGELLSVFMARAAMCLQPTSAGACGFCKSCQLFNAGN
HPDFYHIAADGNQIKVDQIRELCTRLTATAQQSGMRVAIIHHSERLNSAAANALLKTLEEPGKDTLLILQSDTPARLMAT
VSSRCQRIPFVVPSREIIKNWLAQQCDIQEDVTWCLSVVGGPLKLAESLQSEGGQQSRYQSLLQFRKDWAQSLSSGHLCA
SLLTISEQQIIDVLKVLYLLIRQVLLKNSHQDAFIHAQLGNLAAKIMGMCHSLSTMPSVNYLALCQSYVLEYREITNK

Sequences:

>Translated_318_residues
MVAESGFTAAQLPWLEGPRQAFLAQLTRQKMPHAQLVGIDSAYGGELLSVFMARAAMCLQPTSAGACGFCKSCQLFNAGN
HPDFYHIAADGNQIKVDQIRELCTRLTATAQQSGMRVAIIHHSERLNSAAANALLKTLEEPGKDTLLILQSDTPARLMAT
VSSRCQRIPFVVPSREIIKNWLAQQCDIQEDVTWCLSVVGGPLKLAESLQSEGGQQSRYQSLLQFRKDWAQSLSSGHLCA
SLLTISEQQIIDVLKVLYLLIRQVLLKNSHQDAFIHAQLGNLAAKIMGMCHSLSTMPSVNYLALCQSYVLEYREITNK
>Mature_318_residues
MVAESGFTAAQLPWLEGPRQAFLAQLTRQKMPHAQLVGIDSAYGGELLSVFMARAAMCLQPTSAGACGFCKSCQLFNAGN
HPDFYHIAADGNQIKVDQIRELCTRLTATAQQSGMRVAIIHHSERLNSAAANALLKTLEEPGKDTLLILQSDTPARLMAT
VSSRCQRIPFVVPSREIIKNWLAQQCDIQEDVTWCLSVVGGPLKLAESLQSEGGQQSRYQSLLQFRKDWAQSLSSGHLCA
SLLTISEQQIIDVLKVLYLLIRQVLLKNSHQDAFIHAQLGNLAAKIMGMCHSLSTMPSVNYLALCQSYVLEYREITNK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=181, Percent_Identity=34.8066298342541, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786676, Length=136, Percent_Identity=28.6764705882353, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35046; Mature: 35046

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAESGFTAAQLPWLEGPRQAFLAQLTRQKMPHAQLVGIDSAYGGELLSVFMARAAMCLQ
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHHCC
PTSAGACGFCKSCQLFNAGNHPDFYHIAADGNQIKVDQIRELCTRLTATAQQSGMRVAII
CCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEE
HHSERLNSAAANALLKTLEEPGKDTLLILQSDTPARLMATVSSRCQRIPFVVPSREIIKN
ECHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
WLAQQCDIQEDVTWCLSVVGGPLKLAESLQSEGGQQSRYQSLLQFRKDWAQSLSSGHLCA
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
SLLTISEQQIIDVLKVLYLLIRQVLLKNSHQDAFIHAQLGNLAAKIMGMCHSLSTMPSVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHH
YLALCQSYVLEYREITNK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVAESGFTAAQLPWLEGPRQAFLAQLTRQKMPHAQLVGIDSAYGGELLSVFMARAAMCLQ
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHHCC
PTSAGACGFCKSCQLFNAGNHPDFYHIAADGNQIKVDQIRELCTRLTATAQQSGMRVAII
CCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEE
HHSERLNSAAANALLKTLEEPGKDTLLILQSDTPARLMATVSSRCQRIPFVVPSREIIKN
ECHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
WLAQQCDIQEDVTWCLSVVGGPLKLAESLQSEGGQQSRYQSLLQFRKDWAQSLSSGHLCA
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
SLLTISEQQIIDVLKVLYLLIRQVLLKNSHQDAFIHAQLGNLAAKIMGMCHSLSTMPSVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHH
YLALCQSYVLEYREITNK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]