Click here to switch to the map view.

The map label for this gene is purR [H]

Identifier: 160875573

GI number: 160875573

Start: 2911857

End: 2912912

Strand: Reverse

Name: purR [H]

Synonym: Sbal195_2461

Alternate gene names: 160875573

Gene position: 2912912-2911857 (Counterclockwise)

Preceding gene: 160875574

Following gene: 160875571

Centisome position: 54.47

GC content: 48.11

Gene sequence:

>1056_bases
ATGGCATCGAAAGCGACCTCGATTGATATTGCTTATCGTGCTGGCGTATCCCAGTCTACGGTTTCCCGTGCCCTTAGAAA
CAGTCCGTTAGTCAATTTAGAGACCCGTCAACGTATTCAAGCCATTGCTAAAGAGCTGAATTACAAGGTTGATAAAAACG
CCAGTAATCTTCGCACCCAAAATAGCCATACCTTAGCGCTGTTATTGTGTGAAGACCCCACCAATGATGACTCGTTAATC
AATCCCTTTTTTCTGTCTATGCTAGGTTCAATTACCCGAGCAACTGCGCAGCAGGGTTATGATTTACTTGTTTCTTTTCA
GCAATTATCCAGCGATTGGCATGCCGATTATGAAGACAGCAATAAAGCCGATGGCATTATTCTGCTCGGCTATGGCGACT
ACATGGATTACGAGCAAAAGCTGGAGCGACTGTTAGCGCAGAATACCCATTTTGTGATTTGGGGCGCCGAGCATAACAAT
AAGTCTGTGCTATCGATTGGTTGTGATAATCATCAAGGCGGCTTGATTGCGACAGAGCATTTGATTTCCCTTGGTCGCAA
AGCGTTTGCCTTTTTAGGCGATGCTTCGAGCCATAGCCCTGAGTTTAGGGACAGATATTTAGGCCATGTGAAGGCGCTGC
AAACGGCGAATATTCCCGTGGATCGCACTAAGCAAGTGTCGGCCATCAGCACAGAAAGTTCCGGCTACGATGCCGCTATG
GTATTGATTGCCCAAGGCGCTAAGTTTGATGCGATATTTGCGGCCAGCGATTTGATCGCCATCGGCGCGATTCGGGCGCT
GAAAGAAAAGGGCATTTTAGTCCCAGAGCAAGTGGCTGTAGTCGGATACGACGATATTCCGGTGGCGAGTTTTGCTAATC
CGCCATTAACGACCATTAAGCAGAACACCCAATTAGCCGGTGAGATCTTAGTCGACAGTGTACTCAAACTTATCCGCGGG
CAAGAGGTGAGTGCGCAGCTTATTCCGACAACCTTAGTGGTTCGCAGCTCATGCGGCATTGATAGCGCGCCGCTTAAGCC
GTTATCGCTAGCATAA

Upstream 100 bases:

>100_bases
AAATCTAAGTGTAAGACGGATGTTAATGCATTCGTCTATACAAGTTAGCGGAACTGTTTTAGTATCGACGCAAAATAATC
ACTAAGGTTACCTCCTCTCT

Downstream 100 bases:

>100_bases
TGTTTTTGTCGGCAACCGATTGAATCGTTAATTTTACCTGTTGAAACTAAGCTGATGGAACAAAGCCGATAATCCTAGGA
TATATCGGCTTTTTACTATC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQNSHTLALLLCEDPTNDDSLI
NPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDSNKADGIILLGYGDYMDYEQKLERLLAQNTHFVIWGAEHNN
KSVLSIGCDNHQGGLIATEHLISLGRKAFAFLGDASSHSPEFRDRYLGHVKALQTANIPVDRTKQVSAISTESSGYDAAM
VLIAQGAKFDAIFAASDLIAIGAIRALKEKGILVPEQVAVVGYDDIPVASFANPPLTTIKQNTQLAGEILVDSVLKLIRG
QEVSAQLIPTTLVVRSSCGIDSAPLKPLSLA

Sequences:

>Translated_351_residues
MASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQNSHTLALLLCEDPTNDDSLI
NPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDSNKADGIILLGYGDYMDYEQKLERLLAQNTHFVIWGAEHNN
KSVLSIGCDNHQGGLIATEHLISLGRKAFAFLGDASSHSPEFRDRYLGHVKALQTANIPVDRTKQVSAISTESSGYDAAM
VLIAQGAKFDAIFAASDLIAIGAIRALKEKGILVPEQVAVVGYDDIPVASFANPPLTTIKQNTQLAGEILVDSVLKLIRG
QEVSAQLIPTTLVVRSSCGIDSAPLKPLSLA
>Mature_350_residues
ASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQNSHTLALLLCEDPTNDDSLIN
PFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDSNKADGIILLGYGDYMDYEQKLERLLAQNTHFVIWGAEHNNK
SVLSIGCDNHQGGLIATEHLISLGRKAFAFLGDASSHSPEFRDRYLGHVKALQTANIPVDRTKQVSAISTESSGYDAAMV
LIAQGAKFDAIFAASDLIAIGAIRALKEKGILVPEQVAVVGYDDIPVASFANPPLTTIKQNTQLAGEILVDSVLKLIRGQ
EVSAQLIPTTLVVRSSCGIDSAPLKPLSLA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=29.080118694362, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790194, Length=334, Percent_Identity=28.1437125748503, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=27.5659824046921, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=28.9036544850498, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786540, Length=340, Percent_Identity=31.4705882352941, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787948, Length=352, Percent_Identity=26.9886363636364, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789202, Length=302, Percent_Identity=27.4834437086093, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1789456, Length=344, Percent_Identity=26.7441860465116, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787580, Length=330, Percent_Identity=29.0909090909091, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI48994940, Length=327, Percent_Identity=23.8532110091743, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790715, Length=299, Percent_Identity=26.7558528428094, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787906, Length=349, Percent_Identity=24.6418338108883, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790689, Length=342, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38027; Mature: 37895

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQ
CCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCEECCCCHHCCCC
NSHTLALLLCEDPTNDDSLINPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDS
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
NKADGIILLGYGDYMDYEQKLERLLAQNTHFVIWGAEHNNKSVLSIGCDNHQGGLIATEH
CCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHH
LISLGRKAFAFLGDASSHSPEFRDRYLGHVKALQTANIPVDRTKQVSAISTESSGYDAAM
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEE
VLIAQGAKFDAIFAASDLIAIGAIRALKEKGILVPEQVAVVGYDDIPVASFANPPLTTIK
EEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHH
QNTQLAGEILVDSVLKLIRGQEVSAQLIPTTLVVRSSCGIDSAPLKPLSLA
CCHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQ
CCCCCEEEEEEECCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCEECCCCHHCCCC
NSHTLALLLCEDPTNDDSLINPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDS
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
NKADGIILLGYGDYMDYEQKLERLLAQNTHFVIWGAEHNNKSVLSIGCDNHQGGLIATEH
CCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHH
LISLGRKAFAFLGDASSHSPEFRDRYLGHVKALQTANIPVDRTKQVSAISTESSGYDAAM
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEE
VLIAQGAKFDAIFAASDLIAIGAIRALKEKGILVPEQVAVVGYDDIPVASFANPPLTTIK
EEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHH
QNTQLAGEILVDSVLKLIRGQEVSAQLIPTTLVVRSSCGIDSAPLKPLSLA
CCHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA