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The map label for this gene is ruvC

Identifier: 160875533

GI number: 160875533

Start: 2861053

End: 2861574

Strand: Reverse

Name: ruvC

Synonym: Sbal195_2421

Alternate gene names: 160875533

Gene position: 2861574-2861053 (Counterclockwise)

Preceding gene: 160875534

Following gene: 160875532

Centisome position: 53.51

GC content: 50.0

Gene sequence:

>522_bases
ATGGCAATTATTTTAGGTGTTGACCCAGGTTCACGGATCACAGGCTACGGCGTGATCCAATGTCAGGGACGGCATCAGAT
TTACCTCGGCAGTGGCTGTATTCGTACTTCGTCTGAAGAGCTTCCTGGTAGGCTCAAGCAAATTTTTGATGGGATCACTG
AAATTATTCGTCAGTATCAACCGGACGAATTTGCAATTGAGCGAGTATTTATGGCAAAAAATGCCGATTCTGCGCTCAAG
CTTGGTCAAGCACGCGGCGCGGCCATTGTCGCCGCCACGGTCGCCAATTTACCTGTGGCTGAATACAGTGCGACTCAAAT
TAAAAGCGCAGTCGTTGGCACGGGGCGAGCACAGAAAGCTCAAGTACAACACATGATCCAACAACTACTTAAATTACCCG
CAGCCCCACAAGCTGACGCCGCAGATGCACTCGGTGTTGCTGTGTGTCATTATCACACCAGCCAGAGTTTAATTGCATTG
AGTGGCCGGGCAACGGCAAGAACATACGGACGGTACAGATGA

Upstream 100 bases:

>100_bases
GGGCGTCAATCTAGACCAATTTCAGCTCGCACACGCTAACGTAGGACACTCGCTAATCCAGGAACTGAGACGAACATTCA
GATCCCATATAAGGCATTAA

Downstream 100 bases:

>100_bases
TAGGTCGTTTACGGGGCGTGTTAGTTGAGAAACAAGCGCCAGAAATTTTAATCGATGTTAATGGTGTGGGTTACGAGTTG
CAGATGCCGCTCACCAGCTT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MAIILGVDPGSRITGYGVIQCQGRHQIYLGSGCIRTSSEELPGRLKQIFDGITEIIRQYQPDEFAIERVFMAKNADSALK
LGQARGAAIVAATVANLPVAEYSATQIKSAVVGTGRAQKAQVQHMIQQLLKLPAAPQADAADALGVAVCHYHTSQSLIAL
SGRATARTYGRYR

Sequences:

>Translated_173_residues
MAIILGVDPGSRITGYGVIQCQGRHQIYLGSGCIRTSSEELPGRLKQIFDGITEIIRQYQPDEFAIERVFMAKNADSALK
LGQARGAAIVAATVANLPVAEYSATQIKSAVVGTGRAQKAQVQHMIQQLLKLPAAPQADAADALGVAVCHYHTSQSLIAL
SGRATARTYGRYR
>Mature_172_residues
AIILGVDPGSRITGYGVIQCQGRHQIYLGSGCIRTSSEELPGRLKQIFDGITEIIRQYQPDEFAIERVFMAKNADSALKL
GQARGAAIVAATVANLPVAEYSATQIKSAVVGTGRAQKAQVQHMIQQLLKLPAAPQADAADALGVAVCHYHTSQSLIALS
GRATARTYGRYR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=173, Percent_Identity=65.3179190751445, Blast_Score=208, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_SHEB2 (B8EA73)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357969.1
- ProteinModelPortal:   B8EA73
- SMR:   B8EA73
- GeneID:   7086876
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_2042
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- ProtClustDB:   PRK00039
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18496; Mature: 18364

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIILGVDPGSRITGYGVIQCQGRHQIYLGSGCIRTSSEELPGRLKQIFDGITEIIRQYQ
CEEEEECCCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PDEFAIERVFMAKNADSALKLGQARGAAIVAATVANLPVAEYSATQIKSAVVGTGRAQKA
CCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCCHHHH
QVQHMIQQLLKLPAAPQADAADALGVAVCHYHTSQSLIALSGRATARTYGRYR
HHHHHHHHHHHCCCCCCCCCHHHHHHHEEEEECCCCEEEECCCCHHHHCCCCC
>Mature Secondary Structure 
AIILGVDPGSRITGYGVIQCQGRHQIYLGSGCIRTSSEELPGRLKQIFDGITEIIRQYQ
EEEEECCCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PDEFAIERVFMAKNADSALKLGQARGAAIVAATVANLPVAEYSATQIKSAVVGTGRAQKA
CCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCCHHHH
QVQHMIQQLLKLPAAPQADAADALGVAVCHYHTSQSLIALSGRATARTYGRYR
HHHHHHHHHHHCCCCCCCCCHHHHHHHEEEEECCCCEEEECCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA