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The map label for this gene is ruvA

Identifier: 160875532

GI number: 160875532

Start: 2860439

End: 2861056

Strand: Reverse

Name: ruvA

Synonym: Sbal195_2420

Alternate gene names: 160875532

Gene position: 2861056-2860439 (Counterclockwise)

Preceding gene: 160875533

Following gene: 160875531

Centisome position: 53.5

GC content: 46.76

Gene sequence:

>618_bases
ATGATAGGTCGTTTACGGGGCGTGTTAGTTGAGAAACAAGCGCCAGAAATTTTAATCGATGTTAATGGTGTGGGTTACGA
GTTGCAGATGCCGCTCACCAGCTTTTATGAGCTGCCTGAAGTTAACCATGAAACTATGGTCTACACCCACTTTGTGGTGC
GTGAAGATGCACAGTTACTCTATGGTTTTATCACGAAACAGGAAAGAGCCTTATTTCGCTTACTGATCAAGACCAATGGC
GTGGGTCCCAAGCTTGCACTGACCATTTTATCTGGCATGACAGCCGGAGAATTTGTGGGTTGTGTTGAGCGTGATGACAT
AGTGACCTTAGTGAAATTACCCGGTGTGGGTAAGAAGACCGCCGAGCGTTTACTGGTAGAAATGCGCGATAAGCTCAAGA
GCTTGATGGAAGCCTCTGCAGGTTCAGAGCGTGAGTTTGTGCTGCAATCTAATTACTCTCCTGCGCCGACAGTCAATAGC
GCCGAAGAAGATGCCATTTCAGCTTTGATTTCGCTGGGTTATAAACCGCCGCAGGCGAGCAAATCAGTTTCTGCCGCTTA
TAAAGAGGGTATGGATTCTGAAACTCTGATCAAAGCTGCCCTTAAGTCGATGCTTTAA

Upstream 100 bases:

>100_bases
CCGCAGATGCACTCGGTGTTGCTGTGTGTCATTATCACACCAGCCAGAGTTTAATTGCATTGAGTGGCCGGGCAACGGCA
AGAACATACGGACGGTACAG

Downstream 100 bases:

>100_bases
TTGAATAATGATGATAATTAAGATGAAAGCTGCGAGAAGGAAGAACAATGATTGAGGCAGACAGACTCATCCAGCCGCAG
ATCCAAGCTCAAGATGAATC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MIGRLRGVLVEKQAPEILIDVNGVGYELQMPLTSFYELPEVNHETMVYTHFVVREDAQLLYGFITKQERALFRLLIKTNG
VGPKLALTILSGMTAGEFVGCVERDDIVTLVKLPGVGKKTAERLLVEMRDKLKSLMEASAGSEREFVLQSNYSPAPTVNS
AEEDAISALISLGYKPPQASKSVSAAYKEGMDSETLIKAALKSML

Sequences:

>Translated_205_residues
MIGRLRGVLVEKQAPEILIDVNGVGYELQMPLTSFYELPEVNHETMVYTHFVVREDAQLLYGFITKQERALFRLLIKTNG
VGPKLALTILSGMTAGEFVGCVERDDIVTLVKLPGVGKKTAERLLVEMRDKLKSLMEASAGSEREFVLQSNYSPAPTVNS
AEEDAISALISLGYKPPQASKSVSAAYKEGMDSETLIKAALKSML
>Mature_205_residues
MIGRLRGVLVEKQAPEILIDVNGVGYELQMPLTSFYELPEVNHETMVYTHFVVREDAQLLYGFITKQERALFRLLIKTNG
VGPKLALTILSGMTAGEFVGCVERDDIVTLVKLPGVGKKTAERLLVEMRDKLKSLMEASAGSEREFVLQSNYSPAPTVNS
AEEDAISALISLGYKPPQASKSVSAAYKEGMDSETLIKAALKSML

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=205, Percent_Identity=60, Blast_Score=248, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_SHEB2 (B8EA74)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357970.1
- ProteinModelPortal:   B8EA74
- SMR:   B8EA74
- GeneID:   7086877
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_2043
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22483; Mature: 22483

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRLRGVLVEKQAPEILIDVNGVGYELQMPLTSFYELPEVNHETMVYTHFVVREDAQLL
CCCCHHHEEECCCCCEEEEEECCCCEEEECCHHHHHHCCCCCCCEEEEEEHHHHHHHHHH
YGFITKQERALFRLLIKTNGVGPKLALTILSGMTAGEFVGCVERDDIVTLVKLPGVGKKT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCCHHH
AERLLVEMRDKLKSLMEASAGSEREFVLQSNYSPAPTVNSAEEDAISALISLGYKPPQAS
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCH
KSVSAAYKEGMDSETLIKAALKSML
HHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIGRLRGVLVEKQAPEILIDVNGVGYELQMPLTSFYELPEVNHETMVYTHFVVREDAQLL
CCCCHHHEEECCCCCEEEEEECCCCEEEECCHHHHHHCCCCCCCEEEEEEHHHHHHHHHH
YGFITKQERALFRLLIKTNGVGPKLALTILSGMTAGEFVGCVERDDIVTLVKLPGVGKKT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCCHHH
AERLLVEMRDKLKSLMEASAGSEREFVLQSNYSPAPTVNSAEEDAISALISLGYKPPQAS
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCH
KSVSAAYKEGMDSETLIKAALKSML
HHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA