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The map label for this gene is mdeA [H]

Identifier: 160874762

GI number: 160874762

Start: 1986545

End: 1987738

Strand: Direct

Name: mdeA [H]

Synonym: Sbal195_1645

Alternate gene names: 160874762

Gene position: 1986545-1987738 (Clockwise)

Preceding gene: 160874761

Following gene: 160874763

Centisome position: 37.15

GC content: 49.25

Gene sequence:

>1194_bases
ATGCAAGATGAGTCAGGCAAGATGTGGAAAGCGGCAACACAGGTTATTCATGGCGGTCATGAGCGTGAGGCCTTTGGCGC
CTTAGTGACGCCGCTATATCAAAGTGCGACTTTTGTGTTTGAGTCCGCACAGCAAGGTGGTGAGCGATTTGCGGGTAATG
AGCCGGGGTATATTTACACGCGCTTAGGTAATCCGACAACCGCCGAGCTTGAACGTAAAATGGCGATTTTAGAAGGTGCA
GAGGCCGCCGCTGCAACCGCATCTGGTATGGGCGCAGTATCCGCCGCATTACTGGCTAACTTGCAGATGGGCGATCACTT
AGTGGCATCCAATGCCGTATACGGTTGTACTTATGCGCTAATGACGACTCAGTTCGCTCGCTTTGGTATTGAAGTGTCCC
TAGTGGACTTTTCATCGGTAGCAGAAATAGAAGCGGCGATTAAATCCAATACTAAGGTCATTTTCTGTGAAACCCCAGTG
AATCCCCATTTACAGGTGTTTGATTTGGCAGCAATTGCGACTATTGCCAAACGTCATCAGTTGGTCAGTATTGTCGATAA
CACTTTTATGACGCCATTACTGCAGAGACCGCTGGATTTTGGTATCGATTTGGTGATCCACAGTGCGACTAAATATCTGA
ATGGTCATGGCGATGTGATTGCGGGGATAGTGTGTGGCAGCGAAGAACAGCTCCATAAAGTGAAGTATGAAATCTTAAAA
GATATTGGCGCTGTGATATCGCCACATGATGCTTGGCTGATCTTGCGCGGCCTTAAAACCTTAGATGTACGTTTACAGCG
CCATTGCGATAGTGCCCAGCGAGTGGCCGAGTTTTTGGCCGAACATCCCAGTATTAACCGCGTGTATTATCCTGGACTTG
CCTCTCACCCAGGGCATAAATTTATTGGTCAGCAGATGAAACGTGCCGGCGGGGTGATCGCGTTTGAACTGGCGGCAAAT
TTAGAAGAGGCTATGGCCTTTGTGGGGCATTTAAGCTTGTTCTCTATTGCGGTGAGCCTAGGCGATGCAGAATCCCTCAT
TCAACATCCAGCGTCGATGACGCACTCTCCCTATACGCCAGAGGCGAGAGCGGCGGCTGGCATTGGTGATAATCTGCTGC
GAATTTCCATTGGCTTAGAAGATTGTGACGACATTATCGCTGACTTGAGTCAAGCACTCGCGCGTTTGGCATAA

Upstream 100 bases:

>100_bases
TGTTATCCATTGAATAGTCCATAACGCAGGCATTGAGCATCCCAAACGAACAATCAATCAAGAAGGGGTTCAGTTCAATG
CCAATTACAAGGAGCTAGGT

Downstream 100 bases:

>100_bases
CGAGTGTCACTAATTTAAACCACTAACAATTTGAATGATAAAAGGGATGCGATTTAGCATCCCTTTTTCTATGGTTAGGT
CTAGGTTCATTGGGTTAAGA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQDESGKMWKAATQVIHGGHEREAFGALVTPLYQSATFVFESAQQGGERFAGNEPGYIYTRLGNPTTAELERKMAILEGA
EAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTYALMTTQFARFGIEVSLVDFSSVAEIEAAIKSNTKVIFCETPV
NPHLQVFDLAAIATIAKRHQLVSIVDNTFMTPLLQRPLDFGIDLVIHSATKYLNGHGDVIAGIVCGSEEQLHKVKYEILK
DIGAVISPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLAEHPSINRVYYPGLASHPGHKFIGQQMKRAGGVIAFELAAN
LEEAMAFVGHLSLFSIAVSLGDAESLIQHPASMTHSPYTPEARAAAGIGDNLLRISIGLEDCDDIIADLSQALARLA

Sequences:

>Translated_397_residues
MQDESGKMWKAATQVIHGGHEREAFGALVTPLYQSATFVFESAQQGGERFAGNEPGYIYTRLGNPTTAELERKMAILEGA
EAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTYALMTTQFARFGIEVSLVDFSSVAEIEAAIKSNTKVIFCETPV
NPHLQVFDLAAIATIAKRHQLVSIVDNTFMTPLLQRPLDFGIDLVIHSATKYLNGHGDVIAGIVCGSEEQLHKVKYEILK
DIGAVISPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLAEHPSINRVYYPGLASHPGHKFIGQQMKRAGGVIAFELAAN
LEEAMAFVGHLSLFSIAVSLGDAESLIQHPASMTHSPYTPEARAAAGIGDNLLRISIGLEDCDDIIADLSQALARLA
>Mature_397_residues
MQDESGKMWKAATQVIHGGHEREAFGALVTPLYQSATFVFESAQQGGERFAGNEPGYIYTRLGNPTTAELERKMAILEGA
EAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTYALMTTQFARFGIEVSLVDFSSVAEIEAAIKSNTKVIFCETPV
NPHLQVFDLAAIATIAKRHQLVSIVDNTFMTPLLQRPLDFGIDLVIHSATKYLNGHGDVIAGIVCGSEEQLHKVKYEILK
DIGAVISPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLAEHPSINRVYYPGLASHPGHKFIGQQMKRAGGVIAFELAAN
LEEAMAFVGHLSLFSIAVSLGDAESLIQHPASMTHSPYTPEARAAAGIGDNLLRISIGLEDCDDIIADLSQALARLA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=370, Percent_Identity=40.5405405405405, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI299473759, Length=370, Percent_Identity=37.8378378378378, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI25453487, Length=369, Percent_Identity=35.7723577235772, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI1790375, Length=386, Percent_Identity=39.8963730569948, Blast_Score=274, Evalue=7e-75,
Organism=Escherichia coli, GI1789383, Length=400, Percent_Identity=33.75, Blast_Score=201, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI71980708, Length=374, Percent_Identity=39.572192513369, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17560056, Length=386, Percent_Identity=42.4870466321244, Blast_Score=276, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI17538055, Length=386, Percent_Identity=41.4507772020725, Blast_Score=272, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319307, Length=394, Percent_Identity=39.8477157360406, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6323333, Length=426, Percent_Identity=31.924882629108, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321254, Length=407, Percent_Identity=30.958230958231, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6321170, Length=309, Percent_Identity=31.7152103559871, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321904, Length=411, Percent_Identity=28.7104622871046, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI19922554, Length=386, Percent_Identity=39.3782383419689, Blast_Score=273, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42663; Mature: 42663

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDESGKMWKAATQVIHGGHEREAFGALVTPLYQSATFVFESAQQGGERFAGNEPGYIYT
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCEEEE
RLGNPTTAELERKMAILEGAEAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTYAL
ECCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHH
MTTQFARFGIEVSLVDFSSVAEIEAAIKSNTKVIFCETPVNPHLQVFDLAAIATIAKRHQ
HHHHHHHHCCEEEEECHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
LVSIVDNTFMTPLLQRPLDFGIDLVIHSATKYLNGHGDVIAGIVCGSEEQLHKVKYEILK
HHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHH
DIGAVISPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLAEHPSINRVYYPGLASHPGHK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCCCCHH
FIGQQMKRAGGVIAFELAANLEEAMAFVGHLSLFSIAVSLGDAESLIQHPASMTHSPYTP
HHHHHHHHHCCEEEEHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCCCCCC
EARAAAGIGDNLLRISIGLEDCDDIIADLSQALARLA
CHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDESGKMWKAATQVIHGGHEREAFGALVTPLYQSATFVFESAQQGGERFAGNEPGYIYT
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCEEEE
RLGNPTTAELERKMAILEGAEAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTYAL
ECCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHH
MTTQFARFGIEVSLVDFSSVAEIEAAIKSNTKVIFCETPVNPHLQVFDLAAIATIAKRHQ
HHHHHHHHCCEEEEECHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
LVSIVDNTFMTPLLQRPLDFGIDLVIHSATKYLNGHGDVIAGIVCGSEEQLHKVKYEILK
HHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHH
DIGAVISPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLAEHPSINRVYYPGLASHPGHK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCCCCHH
FIGQQMKRAGGVIAFELAANLEEAMAFVGHLSLFSIAVSLGDAESLIQHPASMTHSPYTP
HHHHHHHHHCCEEEEHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCCCCCC
EARAAAGIGDNLLRISIGLEDCDDIIADLSQALARLA
CHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]