Click here to switch to the map view.

The map label for this gene is miaE [H]

Identifier: 160874738

GI number: 160874738

Start: 1959585

End: 1960355

Strand: Direct

Name: miaE [H]

Synonym: Sbal195_1621

Alternate gene names: 160874738

Gene position: 1959585-1960355 (Clockwise)

Preceding gene: 160874736

Following gene: 160874741

Centisome position: 36.65

GC content: 45.27

Gene sequence:

>771_bases
ATGCAGCAACTTCTTGCCCCCATCCACGCGTTTCTTCGCTGTGAAACACCGCAAACTTGGATTGATGTCGCGATAAAATC
AGATTCCTTGGAAGAGTTGCTTATCGATCATTGTAACTGTGAGTTAAAAGCGGCTCAGACGGCGATATTTTTGCTACGAA
AATATGCGTTAGATAAAGACAGTGGTCGAGCTCTGTTGGCGTGGGCTAAACCCTATGAAGAGTTTATCTATTCGCAAGAT
CATGATGTAGTGAGCTTTCTGGCGCGCGATGTGAAAAAGAATGAGCTGGTGGCCGAACTTATGCCTAAGCCAAACTTTAA
GTTTGCCGCTAATTTGGTTTCACCTTTAATCCGCTTGATTAAAGAAGAGTTTCATCATTTTGAGCAAGTATTGGAATTGA
TGCACAAACGCAGCATTGAGTATCGTAATATTCGCGCAGGCCGTTATGCCAAAGGCATGATGAGCTATGTAACCACCCAT
GAGAAAGATATCCTGCGGGATAAGCTGATTGTGGGGGCGTATATTGAGGCGCGTTCGTGCGAGCGTTTTGCTAAGTTGGC
GCCCTATTTAGATGGTGAGCTGAGTAAGTTTTATGTGTCACTGCTGCGCTCTGAAGCCCGCCACTATCAAGATTATCTTG
CATTAGCACAGTTGGTTTCACCTGAGGATATTAGCGAGCGCGTAAAGTACTTTGGTGAGTTAGAGGCTGAGTTGATTCTG
GCGCAGGATGATGAGTTTCGTTTCCATAGTGGATCGCCGCTAGCAAATTGA

Upstream 100 bases:

>100_bases
AAAAAGTTCGGCACGTCAAGTTTACTGAAGCCTAATGCTATAGCTTATGAGTAAATATGCGTAAAATAGCGCGATATTCA
GAATGATATTGAGTGAGTCC

Downstream 100 bases:

>100_bases
TTTGAAGCACCTTCTTTTTAAAAATTGATGAATTAAGTCGATATAGCTAAAACGATTAACGGTTCGCTCGAACCGTTAAT
ATAATGCGTCTTTAACCTCA

Product: tRNA--hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MQQLLAPIHAFLRCETPQTWIDVAIKSDSLEELLIDHCNCELKAAQTAIFLLRKYALDKDSGRALLAWAKPYEEFIYSQD
HDVVSFLARDVKKNELVAELMPKPNFKFAANLVSPLIRLIKEEFHHFEQVLELMHKRSIEYRNIRAGRYAKGMMSYVTTH
EKDILRDKLIVGAYIEARSCERFAKLAPYLDGELSKFYVSLLRSEARHYQDYLALAQLVSPEDISERVKYFGELEAELIL
AQDDEFRFHSGSPLAN

Sequences:

>Translated_256_residues
MQQLLAPIHAFLRCETPQTWIDVAIKSDSLEELLIDHCNCELKAAQTAIFLLRKYALDKDSGRALLAWAKPYEEFIYSQD
HDVVSFLARDVKKNELVAELMPKPNFKFAANLVSPLIRLIKEEFHHFEQVLELMHKRSIEYRNIRAGRYAKGMMSYVTTH
EKDILRDKLIVGAYIEARSCERFAKLAPYLDGELSKFYVSLLRSEARHYQDYLALAQLVSPEDISERVKYFGELEAELIL
AQDDEFRFHSGSPLAN
>Mature_256_residues
MQQLLAPIHAFLRCETPQTWIDVAIKSDSLEELLIDHCNCELKAAQTAIFLLRKYALDKDSGRALLAWAKPYEEFIYSQD
HDVVSFLARDVKKNELVAELMPKPNFKFAANLVSPLIRLIKEEFHHFEQVLELMHKRSIEYRNIRAGRYAKGMMSYVTTH
EKDILRDKLIVGAYIEARSCERFAKLAPYLDGELSKFYVSLLRSEARHYQDYLALAQLVSPEDISERVKYFGELEAELIL
AQDDEFRFHSGSPLAN

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 29660; Mature: 29660

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLLAPIHAFLRCETPQTWIDVAIKSDSLEELLIDHCNCELKAAQTAIFLLRKYALDKD
CHHHHHHHHHHHHCCCCCHHHHEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
SGRALLAWAKPYEEFIYSQDHDVVSFLARDVKKNELVAELMPKPNFKFAANLVSPLIRLI
CCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KEEFHHFEQVLELMHKRSIEYRNIRAGRYAKGMMSYVTTHEKDILRDKLIVGAYIEARSC
HHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERFAKLAPYLDGELSKFYVSLLRSEARHYQDYLALAQLVSPEDISERVKYFGELEAELIL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEE
AQDDEFRFHSGSPLAN
ECCCCCEECCCCCCCC
>Mature Secondary Structure
MQQLLAPIHAFLRCETPQTWIDVAIKSDSLEELLIDHCNCELKAAQTAIFLLRKYALDKD
CHHHHHHHHHHHHCCCCCHHHHEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
SGRALLAWAKPYEEFIYSQDHDVVSFLARDVKKNELVAELMPKPNFKFAANLVSPLIRLI
CCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KEEFHHFEQVLELMHKRSIEYRNIRAGRYAKGMMSYVTTHEKDILRDKLIVGAYIEARSC
HHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERFAKLAPYLDGELSKFYVSLLRSEARHYQDYLALAQLVSPEDISERVKYFGELEAELIL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEE
AQDDEFRFHSGSPLAN
ECCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]