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The map label for this gene is spsK [H]

Identifier: 160874621

GI number: 160874621

Start: 1813935

End: 1815044

Strand: Direct

Name: spsK [H]

Synonym: Sbal195_1504

Alternate gene names: 160874621

Gene position: 1813935-1815044 (Clockwise)

Preceding gene: 160874620

Following gene: 160874622

Centisome position: 33.92

GC content: 49.19

Gene sequence:

>1110_bases
ATGAAAATAGTGGCAATTAAAGTGACACCAACTCAATGCGCTGAGGCGAGCACTACAGAAGCTAACATCCCGCAAGTCGT
GTTAATCACTGGCGCGAAGAGTCAGCTCGCACAGGCTTTGCTGAGAGTCGCTGCCGATATTGGCGAGCTAGCCTTAAATA
GCAACGCAAACACAAACACAAGCATACCGCTGGAGCTTTATGCGTTAAGTCGCAGTCAGTTAGACATTACCGATGCCGCC
AATATTGCAGCAGTGTTTGATCAATATCGACCAAGCTGGGTGATCAATTGCGCGGCCTATAATGCGGTAGACGCTGCTGA
GCATGACGCAATCGAAGCTAATCGGGTGAATGCACTCGGCCCTGAGTTATTGGCGCAGCAATGTTTGTTGTCGGGCGCGC
GCTTACTGCATGTGTCGAGCGATTATGTGTTTGGTGGTCAAGCTGTTTGTGATGTTAGAGAAACTGGCGCTGAGCAGTAT
CAAAATCTAGACTCAGCTCCAAATCATTTACCAAGGCCATTTGTTGAACTTGATGCTCCCGAGCCGTTGTCTGCTTACGG
TACGAGTAAGTTGCTCGGCGAGCAGAAGGTATTGGCGGTTCTGGGCGAGGATGCAACTATTGTGCGTACCTCATGGCTCT
ACGGTCAAGATGGGCATAATTTTGTCCAAACCATGCTGAACTTGATGCGCACTCAACCAAGCTTGGAGGTGATAGTCGAT
CAAATCGGCTGTCCGACGTGGAGTGATGCACTCGCTAAGGTGATTTGGCAATTAGTCATGCAACAGTGCTCGGGCGTATT
TCATTACAGCGCCCAAGGCCAATGCAGTTGGTATGAATTTGCCTGTGAAATCCAGCGACAAGCCCTAGCGCTCAATCTGT
TAAGTCAACCTGTGGTTATCCAGCCGATTACCAGCGCTCACTATGCTAAACAGGCATTAAATCGCGGCATGAGTTTGGCA
AAGAGGCCGAGTTATAGCGTGTTGGATAGTGCCAAAGTGCAGTCCACTTTGGCTGTCAATACCACACTGCCTTTGCCTGA
TAACGTCGTATGGCAAGATTGGCGTCAGCAACTGAATTGCATGTTGCGACGCTATCATGACGATAATTAG

Upstream 100 bases:

>100_bases
TCCTTCTGCACTCTTGGCCTTTTTAGCCGCCCGTGGTCATGAAACCGTCGCCGATTTGATCCCCTTATTTAGCGATGATG
AGCCATGCTGTTGATCCGTG

Downstream 100 bases:

>100_bases
CGTGACTCACATTTGGGTTTAGGCAGGTTTAATCAGGCGTTAAGCGTTTACATCATCGTAGTGATTAACGCTGTCGAAAA
CAATAAGCCCGCCAATAATC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKIVAIKVTPTQCAEASTTEANIPQVVLITGAKSQLAQALLRVAADIGELALNSNANTNTSIPLELYALSRSQLDITDAA
NIAAVFDQYRPSWVINCAAYNAVDAAEHDAIEANRVNALGPELLAQQCLLSGARLLHVSSDYVFGGQAVCDVRETGAEQY
QNLDSAPNHLPRPFVELDAPEPLSAYGTSKLLGEQKVLAVLGEDATIVRTSWLYGQDGHNFVQTMLNLMRTQPSLEVIVD
QIGCPTWSDALAKVIWQLVMQQCSGVFHYSAQGQCSWYEFACEIQRQALALNLLSQPVVIQPITSAHYAKQALNRGMSLA
KRPSYSVLDSAKVQSTLAVNTTLPLPDNVVWQDWRQQLNCMLRRYHDDN

Sequences:

>Translated_369_residues
MKIVAIKVTPTQCAEASTTEANIPQVVLITGAKSQLAQALLRVAADIGELALNSNANTNTSIPLELYALSRSQLDITDAA
NIAAVFDQYRPSWVINCAAYNAVDAAEHDAIEANRVNALGPELLAQQCLLSGARLLHVSSDYVFGGQAVCDVRETGAEQY
QNLDSAPNHLPRPFVELDAPEPLSAYGTSKLLGEQKVLAVLGEDATIVRTSWLYGQDGHNFVQTMLNLMRTQPSLEVIVD
QIGCPTWSDALAKVIWQLVMQQCSGVFHYSAQGQCSWYEFACEIQRQALALNLLSQPVVIQPITSAHYAKQALNRGMSLA
KRPSYSVLDSAKVQSTLAVNTTLPLPDNVVWQDWRQQLNCMLRRYHDDN
>Mature_369_residues
MKIVAIKVTPTQCAEASTTEANIPQVVLITGAKSQLAQALLRVAADIGELALNSNANTNTSIPLELYALSRSQLDITDAA
NIAAVFDQYRPSWVINCAAYNAVDAAEHDAIEANRVNALGPELLAQQCLLSGARLLHVSSDYVFGGQAVCDVRETGAEQY
QNLDSAPNHLPRPFVELDAPEPLSAYGTSKLLGEQKVLAVLGEDATIVRTSWLYGQDGHNFVQTMLNLMRTQPSLEVIVD
QIGCPTWSDALAKVIWQLVMQQCSGVFHYSAQGQCSWYEFACEIQRQALALNLLSQPVVIQPITSAHYAKQALNRGMSLA
KRPSYSVLDSAKVQSTLAVNTTLPLPDNVVWQDWRQQLNCMLRRYHDDN

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=377, Percent_Identity=26.525198938992, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI33519455, Length=350, Percent_Identity=26.5714285714286, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788352, Length=338, Percent_Identity=29.8816568047337, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 40424; Mature: 40424

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVAIKVTPTQCAEASTTEANIPQVVLITGAKSQLAQALLRVAADIGELALNSNANTNT
CEEEEEEECCHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SIPLELYALSRSQLDITDAANIAAVFDQYRPSWVINCAAYNAVDAAEHDAIEANRVNALG
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCC
PELLAQQCLLSGARLLHVSSDYVFGGQAVCDVRETGAEQYQNLDSAPNHLPRPFVELDAP
HHHHHHHHHHCCCEEEEECCCEEECCCEEEHHHHHHHHHHHCCCCCCCCCCCCCEECCCC
EPLSAYGTSKLLGEQKVLAVLGEDATIVRTSWLYGQDGHNFVQTMLNLMRTQPSLEVIVD
CCHHHHCCHHHHCCHHEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHH
QIGCPTWSDALAKVIWQLVMQQCSGVFHYSAQGQCSWYEFACEIQRQALALNLLSQPVVI
HHCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
QPITSAHYAKQALNRGMSLAKRPSYSVLDSAKVQSTLAVNTTLPLPDNVVWQDWRQQLNC
EECHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHH
MLRRYHDDN
HHHHHCCCC
>Mature Secondary Structure
MKIVAIKVTPTQCAEASTTEANIPQVVLITGAKSQLAQALLRVAADIGELALNSNANTNT
CEEEEEEECCHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SIPLELYALSRSQLDITDAANIAAVFDQYRPSWVINCAAYNAVDAAEHDAIEANRVNALG
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCC
PELLAQQCLLSGARLLHVSSDYVFGGQAVCDVRETGAEQYQNLDSAPNHLPRPFVELDAP
HHHHHHHHHHCCCEEEEECCCEEECCCEEEHHHHHHHHHHHCCCCCCCCCCCCCEECCCC
EPLSAYGTSKLLGEQKVLAVLGEDATIVRTSWLYGQDGHNFVQTMLNLMRTQPSLEVIVD
CCHHHHCCHHHHCCHHEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHH
QIGCPTWSDALAKVIWQLVMQQCSGVFHYSAQGQCSWYEFACEIQRQALALNLLSQPVVI
HHCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
QPITSAHYAKQALNRGMSLAKRPSYSVLDSAKVQSTLAVNTTLPLPDNVVWQDWRQQLNC
EECHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHH
MLRRYHDDN
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]