Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 160874351

GI number: 160874351

Start: 1466677

End: 1467927

Strand: Reverse

Name: cca [H]

Synonym: Sbal195_1232

Alternate gene names: 160874351

Gene position: 1467927-1466677 (Counterclockwise)

Preceding gene: 160874354

Following gene: 160874350

Centisome position: 27.45

GC content: 48.28

Gene sequence:

>1251_bases
GTGAAAATATATCTCGTCGGCGGTGCTGTGCGTGACAGCCTGCTCAATCTCCCTATCAAGGACAAAGATTACCTCGTCGT
TGGTGCGACTCCAGAACAAATGTTGCAACTGGGATACCGCCAAGTCGGTAAAGATTTCCCAGTATTTCTGCATCCTAAAA
ACCAACAAGAATATGCGTTAGCCCGAACAGAACGAAAAATTGCACTGGGTTATGGTGGTTTTAGCTGCCATGCAAGTCCC
GATGTGACTTTAGAGCAAGACTTGTTGCGCCGAGATCTGACCATCAATGCTATCGCCCAAGATGAAAAGGGCAACTTATA
CGATCCTTTTAATGGCATTGAGGATATTAACGCGCGCCTATTGCGCCATGTGTCAGATGCTTTCGTTGAAGATCCGCTTC
GCGTATTGCGTGTCGCCCGCTTTGCCGCAAGGTTTCATGCCTTAGGATTTCACATTGCCGCAGAAACTCTGGCACTGATG
CGCCAAATTAGCGCAAGCGATGAGCTTAACGCGTTAACAGCGGAGCGAGTTTGGCAAGAAGTCGATAAAAGCTTAGGCGG
ACCTCACCCAGAGGTCTTTTTCGAGGTACTGCACCAATGCGGCGCCCTTGAAGTACTCTTTCCCGAGATCTTTGCCTTAT
TTGGCGTTCCTCAACCAGAAAAATGGCATCCTGAAATTGATACTGGTGTGCATACTTTAATGGTGCTCGCCCAAGCCGCC
CTTCTCACTGAGGATAAAAGCGTCCGTTTTGCCGCTCTCGTACATGATTTAGGCAAAGCCCTCAGTCCAAAAGAGCATTT
ACCTAAACATCACGGTCACGGCCAAAAGGGCTTGCCGCTGATAAAAGCGCTCTGCACCCGTTTACGAGTGCCGAACGAAA
CCCGCGATTTGGCGTTACTGGTCAGTGACCAACATCAGAACGTTCATCAGGCCTTCGAGCTTAGAGCCGAAACGATCGTA
AAAATATTCGATAAAGCTGACTTTTGGCGTAAGCCAGAGCGCTTGACGCAGCTTATATTAGCCTGCACCGCCGATATGCG
TGGTCGCACAGGATTTGAAAACAACCCCTACCCTCAGGGTGAATATTTAACTCAATGCTTCCTTGCCGCCAATAATGTCG
ATATTGCCGCCATTATTGCCGCAGGTTTTCAAGGCGCAGAGATAAAGCAGGCGTTAAATTTACGTCGGATTGAAGCGGTC
AGTCAGTTTAAACAAAAAATGCAAACTAAACTGCCGACGGATGAACAGTAA

Upstream 100 bases:

>100_bases
AATGTGACACATAAGTGCGCAAATATCTCGCGACGACTTTATCTATCACCTTAATTGGCATGATAAGCTATGTTTATGAC
ACATTAGCCGAATCTTGCCT

Downstream 100 bases:

>100_bases
TTTGTAAAGGATTTGTAAGTGGCGCATCACTTTATAGTGAAAAATACCCTCCTTTACGATTAGAAATGTCGATATAACTT
ATAAAAAAAGCTTTTAAAAA

Product: polynucleotide adenylyltransferase/metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKIYLVGGAVRDSLLNLPIKDKDYLVVGATPEQMLQLGYRQVGKDFPVFLHPKNQQEYALARTERKIALGYGGFSCHASP
DVTLEQDLLRRDLTINAIAQDEKGNLYDPFNGIEDINARLLRHVSDAFVEDPLRVLRVARFAARFHALGFHIAAETLALM
RQISASDELNALTAERVWQEVDKSLGGPHPEVFFEVLHQCGALEVLFPEIFALFGVPQPEKWHPEIDTGVHTLMVLAQAA
LLTEDKSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKALCTRLRVPNETRDLALLVSDQHQNVHQAFELRAETIV
KIFDKADFWRKPERLTQLILACTADMRGRTGFENNPYPQGEYLTQCFLAANNVDIAAIIAAGFQGAEIKQALNLRRIEAV
SQFKQKMQTKLPTDEQ

Sequences:

>Translated_416_residues
MKIYLVGGAVRDSLLNLPIKDKDYLVVGATPEQMLQLGYRQVGKDFPVFLHPKNQQEYALARTERKIALGYGGFSCHASP
DVTLEQDLLRRDLTINAIAQDEKGNLYDPFNGIEDINARLLRHVSDAFVEDPLRVLRVARFAARFHALGFHIAAETLALM
RQISASDELNALTAERVWQEVDKSLGGPHPEVFFEVLHQCGALEVLFPEIFALFGVPQPEKWHPEIDTGVHTLMVLAQAA
LLTEDKSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKALCTRLRVPNETRDLALLVSDQHQNVHQAFELRAETIV
KIFDKADFWRKPERLTQLILACTADMRGRTGFENNPYPQGEYLTQCFLAANNVDIAAIIAAGFQGAEIKQALNLRRIEAV
SQFKQKMQTKLPTDEQ
>Mature_416_residues
MKIYLVGGAVRDSLLNLPIKDKDYLVVGATPEQMLQLGYRQVGKDFPVFLHPKNQQEYALARTERKIALGYGGFSCHASP
DVTLEQDLLRRDLTINAIAQDEKGNLYDPFNGIEDINARLLRHVSDAFVEDPLRVLRVARFAARFHALGFHIAAETLALM
RQISASDELNALTAERVWQEVDKSLGGPHPEVFFEVLHQCGALEVLFPEIFALFGVPQPEKWHPEIDTGVHTLMVLAQAA
LLTEDKSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKALCTRLRVPNETRDLALLVSDQHQNVHQAFELRAETIV
KIFDKADFWRKPERLTQLILACTADMRGRTGFENNPYPQGEYLTQCFLAANNVDIAAIIAAGFQGAEIKQALNLRRIEAV
SQFKQKMQTKLPTDEQ

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=201, Percent_Identity=30.8457711442786, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789436, Length=406, Percent_Identity=61.576354679803, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71995920, Length=262, Percent_Identity=28.2442748091603, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=239, Percent_Identity=28.4518828451883, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281360112, Length=214, Percent_Identity=28.9719626168224, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21357337, Length=214, Percent_Identity=28.9719626168224, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46681; Mature: 46681

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDSLLNLPIKDKDYLVVGATPEQMLQLGYRQVGKDFPVFLHPKNQQEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKIALGYGGFSCHASPDVTLEQDLLRRDLTINAIAQDEKGNLYDPFNGIEDINARL
HHHCCEEEEECCCEECCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHH
LRHVSDAFVEDPLRVLRVARFAARFHALGFHIAAETLALMRQISASDELNALTAERVWQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VDKSLGGPHPEVFFEVLHQCGALEVLFPEIFALFGVPQPEKWHPEIDTGVHTLMVLAQAA
HHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LLTEDKSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKALCTRLRVPNETRDLALL
HHCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEE
VSDQHQNVHQAFELRAETIVKIFDKADFWRKPERLTQLILACTADMRGRTGFENNPYPQG
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
EYLTQCFLAANNVDIAAIIAAGFQGAEIKQALNLRRIEAVSQFKQKMQTKLPTDEQ
HHHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKIYLVGGAVRDSLLNLPIKDKDYLVVGATPEQMLQLGYRQVGKDFPVFLHPKNQQEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKIALGYGGFSCHASPDVTLEQDLLRRDLTINAIAQDEKGNLYDPFNGIEDINARL
HHHCCEEEEECCCEECCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHH
LRHVSDAFVEDPLRVLRVARFAARFHALGFHIAAETLALMRQISASDELNALTAERVWQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VDKSLGGPHPEVFFEVLHQCGALEVLFPEIFALFGVPQPEKWHPEIDTGVHTLMVLAQAA
HHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LLTEDKSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKALCTRLRVPNETRDLALL
HHCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEE
VSDQHQNVHQAFELRAETIVKIFDKADFWRKPERLTQLILACTADMRGRTGFENNPYPQG
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
EYLTQCFLAANNVDIAAIIAAGFQGAEIKQALNLRRIEAVSQFKQKMQTKLPTDEQ
HHHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA