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The map label for this gene is dnaG [H]

Identifier: 160874341

GI number: 160874341

Start: 1458171

End: 1459895

Strand: Reverse

Name: dnaG [H]

Synonym: Sbal195_1222

Alternate gene names: 160874341

Gene position: 1459895-1458171 (Counterclockwise)

Preceding gene: 160874342

Following gene: 160874340

Centisome position: 27.3

GC content: 45.1

Gene sequence:

>1725_bases
ATGGCAATACCTCGTGATTTTATCAATGAGCTAATCGCTCGCACTGACATAGTCGAACTTATCGATCGTAAGGTGCCCTT
GAAAAAGGCGGGTAAGAACTACTCGGCCTGTTGTCCTTTTCATAGCGAAAAATCACCTTCGTTTACCGTCAGCCGTGATA
AACAGTTCTATCATTGCTTTGGCTGCGGTGCTCACGGCAACGCCATCGATTTTGTGATGGAGTATGACCGACTCGATTTT
ATTGATGCCATTGAAGATTTGGCGGGCCAGCTTGGATTAGAGGTTCCCAGAGAACAAGGCACAGGCAAGCGACATGATGA
CGGTTTAAGTCGTGACTTATACCAACTCATGGAAGAAGCGAGCCTTTACTTTCAAAACCAACTTAGACAACACCAAGATA
AACAAAAAGTTATCGATTATCTTGCCTATCGCGGTTTATCCGATGATGTAGTCGAACATTTTGGTATTGGCTTTGCGCCA
GACGGTTGGGACGGCTTATTGGGCCGCTATCGTCAGAATCAAGATGCGCAGGATAAGTTACTGACTGCGGGAATGTTAAT
TAGCAACGACAGTGGGAAACGTTACGACAGATTTCGTGACCGCCTCATGTTTCCTATCCGCGACAGACGCGGACGAGTCA
TTGGTTTTGGTGGCAGAGTTTTAGGTGATGGCACACCCAAATACTTGAATTCTCCAGAAACGCCCATATTTCATAAGGGC
AATGAGCTTTATGGTCTCTATGAACTAAAGCAACGCTATCGCGATCCTGACAAAGTACTGATCGTTGAAGGCTATATGGA
TGTGGTGGCGTTGGCGCAATTTGGCGTCGATTATGCTGTAGCGTCATTAGGCACCTCGACGACGGCGGAGCAATTTCAGC
TTTTGCTGCGCAGCGCCAAAGAAGTGATTTGCTGTTACGACGGCGACAGAGCGGGTACTGAAGCGGCTTGGCGTGCCTTA
GAAACGGCATTGCCATTATTAAAGCCAGGCGATCAAGTGCGATTTATGTTCCTGCCTCAGGCCGAAGATCCTGATTCTAT
GGTACGTAAGATTGGTAAAGATGCATTTGAAACCTTAATGGTTTCAGCCATTGCACTACCCGAGTTTCTATTTGATACGT
TAGCGACAAAATACGGTACAGATAAAGGCAACTTAGCGAAACAAGCTTTTATCTTAATTGAAAAAATTCAAGACACTGTG
CTGCAAAATTTACTACTTGAGAACTTAGCTCACAAGTTAGGAATGAACAGCTCAGAAGATATGAAAAAGAAGCTAGGCTT
TAGCGTGAAACAAGCTAAACCAATGGCAAATAAAGGATTAAAAGGACGCGGTACACCATTGCGACTCGCCATCGCACTGC
TAGTACAACATCCTAAACTGGGTATGGGCTTACCCCCACAACCCGCACTCGCCCACTTGCAGATGCCAGGCATAGAATTG
CTGCCACTGTTATTGGAGATGACTCGAGAGCAAACGTTAAACAGCGCACAACTACTTGAACAATTCAGAGAGCACCCTTA
CAGCGGCACTCTACATAAATTGGCCCAATGGGACCATCAAGTGGCGGATGAAAACTTGCTCGAAAAGTTTAAACAAACCC
TGGTTTGGTTGAACAATCAATATATTGAACAACGATATCAGGAATTGAGTCTAAAACAGACTCATACTAAAGAAGAGAAG
ATGCAGCTCTCGAAGCTGATCTCAGTCATGAAAGGGTTAAACTGA

Upstream 100 bases:

>100_bases
GTGCTAAATTGCAATAATTAGCGTTTCACTGTATGAACAAGCCGTGCCTATGCGCGGCTTGTTTGTTTAAATTCGCGCAA
GGCGGGATTTTAAGCACTCA

Downstream 100 bases:

>100_bases
GGCAAAAATGTCCTCATTCACTGTCCCAGTGACTAGCACAGACATGTGCACACGGCTATAATTGACGATTTGCGCGGCAC
TTAGCTCATGCTAAGTGTCC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MAIPRDFINELIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLDF
IDAIEDLAGQLGLEVPREQGTGKRHDDGLSRDLYQLMEEASLYFQNQLRQHQDKQKVIDYLAYRGLSDDVVEHFGIGFAP
DGWDGLLGRYRQNQDAQDKLLTAGMLISNDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
NELYGLYELKQRYRDPDKVLIVEGYMDVVALAQFGVDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDRAGTEAAWRAL
ETALPLLKPGDQVRFMFLPQAEDPDSMVRKIGKDAFETLMVSAIALPEFLFDTLATKYGTDKGNLAKQAFILIEKIQDTV
LQNLLLENLAHKLGMNSSEDMKKKLGFSVKQAKPMANKGLKGRGTPLRLAIALLVQHPKLGMGLPPQPALAHLQMPGIEL
LPLLLEMTREQTLNSAQLLEQFREHPYSGTLHKLAQWDHQVADENLLEKFKQTLVWLNNQYIEQRYQELSLKQTHTKEEK
MQLSKLISVMKGLN

Sequences:

>Translated_574_residues
MAIPRDFINELIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLDF
IDAIEDLAGQLGLEVPREQGTGKRHDDGLSRDLYQLMEEASLYFQNQLRQHQDKQKVIDYLAYRGLSDDVVEHFGIGFAP
DGWDGLLGRYRQNQDAQDKLLTAGMLISNDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
NELYGLYELKQRYRDPDKVLIVEGYMDVVALAQFGVDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDRAGTEAAWRAL
ETALPLLKPGDQVRFMFLPQAEDPDSMVRKIGKDAFETLMVSAIALPEFLFDTLATKYGTDKGNLAKQAFILIEKIQDTV
LQNLLLENLAHKLGMNSSEDMKKKLGFSVKQAKPMANKGLKGRGTPLRLAIALLVQHPKLGMGLPPQPALAHLQMPGIEL
LPLLLEMTREQTLNSAQLLEQFREHPYSGTLHKLAQWDHQVADENLLEKFKQTLVWLNNQYIEQRYQELSLKQTHTKEEK
MQLSKLISVMKGLN
>Mature_573_residues
AIPRDFINELIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLDFI
DAIEDLAGQLGLEVPREQGTGKRHDDGLSRDLYQLMEEASLYFQNQLRQHQDKQKVIDYLAYRGLSDDVVEHFGIGFAPD
GWDGLLGRYRQNQDAQDKLLTAGMLISNDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKGN
ELYGLYELKQRYRDPDKVLIVEGYMDVVALAQFGVDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDRAGTEAAWRALE
TALPLLKPGDQVRFMFLPQAEDPDSMVRKIGKDAFETLMVSAIALPEFLFDTLATKYGTDKGNLAKQAFILIEKIQDTVL
QNLLLENLAHKLGMNSSEDMKKKLGFSVKQAKPMANKGLKGRGTPLRLAIALLVQHPKLGMGLPPQPALAHLQMPGIELL
PLLLEMTREQTLNSAQLLEQFREHPYSGTLHKLAQWDHQVADENLLEKFKQTLVWLNNQYIEQRYQELSLKQTHTKEEKM
QLSKLISVMKGLN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=574, Percent_Identity=50.3484320557491, Blast_Score=555, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65049; Mature: 64917

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPRDFINELIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCF
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCEEEECCCCEEEEE
GCGAHGNAIDFVMEYDRLDFIDAIEDLAGQLGLEVPREQGTGKRHDDGLSRDLYQLMEEA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHH
SLYFQNQLRQHQDKQKVIDYLAYRGLSDDVVEHFGIGFAPDGWDGLLGRYRQNQDAQDKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHH
LTAGMLISNDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
HHHHHEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCEEECCCCCHHCCCCCCCCEECC
NELYGLYELKQRYRDPDKVLIVEGYMDVVALAQFGVDYAVASLGTSTTAEQFQLLLRSAK
CCEEHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EVICCYDGDRAGTEAAWRALETALPLLKPGDQVRFMFLPQAEDPDSMVRKIGKDAFETLM
CEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
VSAIALPEFLFDTLATKYGTDKGNLAKQAFILIEKIQDTVLQNLLLENLAHKLGMNSSED
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
MKKKLGFSVKQAKPMANKGLKGRGTPLRLAIALLVQHPKLGMGLPPQPALAHLQMPGIEL
HHHHHCCCHHHCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCHHH
LPLLLEMTREQTLNSAQLLEQFREHPYSGTLHKLAQWDHQVADENLLEKFKQTLVWLNNQ
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIEQRYQELSLKQTHTKEEKMQLSKLISVMKGLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIPRDFINELIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCF
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCEEEECCCCEEEEE
GCGAHGNAIDFVMEYDRLDFIDAIEDLAGQLGLEVPREQGTGKRHDDGLSRDLYQLMEEA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHH
SLYFQNQLRQHQDKQKVIDYLAYRGLSDDVVEHFGIGFAPDGWDGLLGRYRQNQDAQDKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHH
LTAGMLISNDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
HHHHHEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCEEECCCCCHHCCCCCCCCEECC
NELYGLYELKQRYRDPDKVLIVEGYMDVVALAQFGVDYAVASLGTSTTAEQFQLLLRSAK
CCEEHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EVICCYDGDRAGTEAAWRALETALPLLKPGDQVRFMFLPQAEDPDSMVRKIGKDAFETLM
CEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
VSAIALPEFLFDTLATKYGTDKGNLAKQAFILIEKIQDTVLQNLLLENLAHKLGMNSSED
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
MKKKLGFSVKQAKPMANKGLKGRGTPLRLAIALLVQHPKLGMGLPPQPALAHLQMPGIEL
HHHHHCCCHHHCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCHHH
LPLLLEMTREQTLNSAQLLEQFREHPYSGTLHKLAQWDHQVADENLLEKFKQTLVWLNNQ
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIEQRYQELSLKQTHTKEEKMQLSKLISVMKGLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]