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The map label for this gene is rpoD [H]

Identifier: 160874340

GI number: 160874340

Start: 1456200

End: 1458083

Strand: Reverse

Name: rpoD [H]

Synonym: Sbal195_1221

Alternate gene names: 160874340

Gene position: 1458083-1456200 (Counterclockwise)

Preceding gene: 160874341

Following gene: 160874335

Centisome position: 27.27

GC content: 46.5

Gene sequence:

>1884_bases
ATGCTAAGTGTCCGTCGTTGTAACAGTTTCCCAACTTGGATGATATCTATGGATCATACTCCGCAGTCGCAACTTAAGCT
GTTGCTTGCCAAAGGTAAAGAGCAAGGTTACTTAACCTATGCAGAAGTGAACGATCACTTACCTGCAGACATGGTCGATT
CTGACCAGATCGAAGATATTATCCAGATGATAAATGACATGGGTATCCGGGTGTTCGAAGAAGCCCCTGATGCCGATGAC
ATGATGATGTCGGAAGACAACACAGACGAAGATGCCGCTGAAGAAGCCGCTGCCGCACTGGCAACAGTAGAAAGCGAACT
TGGCCGCACAACCGATCCCGTGCGCATGTACATGCGTGAAATGGGTACCGTTGAGCTTCTGACCCGCGAAGGCGAAATTG
TTATCGCCAAACGCATCGAAGAAGGCATCAACACAGTTCAAAGTTCAGTCGCCGAATACCCACAAGCGATCGCTATGATC
CTTGAGCAATTCGACCAATTTGAAGCCGACGAACTGCGCTTATCTGATATTATCTCTGGATTTGTTAACCCTGACGAAGA
CGTTGCACCGACCGCCACTCACATCGGCTCAGAATTATCTGAAGAAGAGCTGGAAGATGAAGATGACGAAGAAGATGACG
ACGACACTGAAGATGAAGAAGAAGGCAACAAAGGGCCTGATCCTGAAGAAGCACGCGAAAGATTCGGTCAACTGAGAACT
GCCTACGAAAGTGCACTGAAAATTATCGAAGCTAAGGGCCGTGAACACCCTGAATCGGTAAAAGCACTATTCGAAATTGG
TGAAATCTTCAAAGAATTCCGCTTAGTACCTAAACAGTTTGACCGTTTAGTGAAAAGCATGCGCACTATGATGGATCGCG
TACGTGTTCAAGAACGTTTAGTGTTAAAACTGTGCGTTGAACAAGCCAAAATGCCGAAGAAAAACTTCGTCAAACTCTTC
ACTGGCAACGAAACCAACCTCGATTGGTTCAACACTGAGAAAGCCTCAACTAAACCTTATGCCGAAGGCCTAAGAATGGT
TGAAGACGACGTGATCCGTTGTCGTACTAAGCTTGCAGCAGTTGAAGAAGAAACCGGCTTAGCAATTTCTGCCATTAAAG
ACATCAACCGTCGTATGTCAATCGGTGAAGCTAAAGCCCGCCGTGCGAAGAAAGAAATGGTTGAAGCAAACTTACGTCTG
GTAATTTCTATCGCGAAGAAATACACCAACCGTGGTTTGCAATTCTTGGACTTAATCCAAGAAGGTAACATTGGCTTGAT
GAAAGCCGTGGATAAATTCGAATACCGTCGTGGCTATAAGTTCTCGACCTATGCAACTTGGTGGATCCGTCAGGCGATTA
CTCGTTCTATTGCCGACCAAGCGCGTACGATCCGTATTCCAGTACACATGATCGAAACGATCAACAAGCTGAACCGTATC
TCTCGCCAAATGCTACAGGAAATGGGCCGCGAGCCTTCACCTGAAGAACTGGCTGAGCGTATGATGATGCCGGAAGATAA
GATCCGTAAGGTACTGAAAATCGCTAAAGAACCTATCTCCATGGAAACCCCAATCGGTGACGATGAAGATTCGCATTTAG
GTGATTTTATCGAGGATACTACCCTCGAACTACCACTGGATTCAGCGACCAGCGAAAGCTTGAAAAACGCCACTCACGAA
GTGTTAGCTGGTCTAACCGCCCGTGAAGCGAAAGTACTGCGTATGCGTTTTGGTATCGATATGAACACTGACCACACACT
TGAAGAAGTGGGTAAGCAGTTCGACGTGACCCGTGAACGTATTCGTCAGATTGAAGCTAAGGCACTGCGCAAACTGCGTC
ACCCTTCACGCTCAGAAATCTTAAAATCATTCCTAGATGAATAG

Upstream 100 bases:

>100_bases
AGGGTTAAACTGAGGCAAAAATGTCCTCATTCACTGTCCCAGTGACTAGCACAGACATGTGCACACGGCTATAATTGACG
ATTTGCGCGGCACTTAGCTC

Downstream 100 bases:

>100_bases
GCCGTTATAAGCCTAAAGTCAGCTAAGTGAGATTTGAAGCCCTGCCTATGCAGGGCTTTTTATTGCCTATCCGGCGGCAA
TATCCGCATAAGCACGCTAT

Product: RNA polymerase sigma-70 subunit RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MLSVRRCNSFPTWMISMDHTPQSQLKLLLAKGKEQGYLTYAEVNDHLPADMVDSDQIEDIIQMINDMGIRVFEEAPDADD
MMMSEDNTDEDAAEEAAAALATVESELGRTTDPVRMYMREMGTVELLTREGEIVIAKRIEEGINTVQSSVAEYPQAIAMI
LEQFDQFEADELRLSDIISGFVNPDEDVAPTATHIGSELSEEELEDEDDEEDDDDTEDEEEGNKGPDPEEARERFGQLRT
AYESALKIIEAKGREHPESVKALFEIGEIFKEFRLVPKQFDRLVKSMRTMMDRVRVQERLVLKLCVEQAKMPKKNFVKLF
TGNETNLDWFNTEKASTKPYAEGLRMVEDDVIRCRTKLAAVEEETGLAISAIKDINRRMSIGEAKARRAKKEMVEANLRL
VISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRI
SRQMLQEMGREPSPEELAERMMMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSESLKNATHE
VLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEILKSFLDE

Sequences:

>Translated_627_residues
MLSVRRCNSFPTWMISMDHTPQSQLKLLLAKGKEQGYLTYAEVNDHLPADMVDSDQIEDIIQMINDMGIRVFEEAPDADD
MMMSEDNTDEDAAEEAAAALATVESELGRTTDPVRMYMREMGTVELLTREGEIVIAKRIEEGINTVQSSVAEYPQAIAMI
LEQFDQFEADELRLSDIISGFVNPDEDVAPTATHIGSELSEEELEDEDDEEDDDDTEDEEEGNKGPDPEEARERFGQLRT
AYESALKIIEAKGREHPESVKALFEIGEIFKEFRLVPKQFDRLVKSMRTMMDRVRVQERLVLKLCVEQAKMPKKNFVKLF
TGNETNLDWFNTEKASTKPYAEGLRMVEDDVIRCRTKLAAVEEETGLAISAIKDINRRMSIGEAKARRAKKEMVEANLRL
VISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRI
SRQMLQEMGREPSPEELAERMMMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSESLKNATHE
VLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEILKSFLDE
>Mature_627_residues
MLSVRRCNSFPTWMISMDHTPQSQLKLLLAKGKEQGYLTYAEVNDHLPADMVDSDQIEDIIQMINDMGIRVFEEAPDADD
MMMSEDNTDEDAAEEAAAALATVESELGRTTDPVRMYMREMGTVELLTREGEIVIAKRIEEGINTVQSSVAEYPQAIAMI
LEQFDQFEADELRLSDIISGFVNPDEDVAPTATHIGSELSEEELEDEDDEEDDDDTEDEEEGNKGPDPEEARERFGQLRT
AYESALKIIEAKGREHPESVKALFEIGEIFKEFRLVPKQFDRLVKSMRTMMDRVRVQERLVLKLCVEQAKMPKKNFVKLF
TGNETNLDWFNTEKASTKPYAEGLRMVEDDVIRCRTKLAAVEEETGLAISAIKDINRRMSIGEAKARRAKKEMVEANLRL
VISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRI
SRQMLQEMGREPSPEELAERMMMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSESLKNATHE
VLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEILKSFLDE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=613, Percent_Identity=77.9771615008157, Blast_Score=947, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=237, Percent_Identity=43.4599156118143, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=28.5123966942149, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.2178217821782, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 71760; Mature: 71760

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVRRCNSFPTWMISMDHTPQSQLKLLLAKGKEQGYLTYAEVNDHLPADMVDSDQIEDI
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHH
IQMINDMGIRVFEEAPDADDMMMSEDNTDEDAAEEAAAALATVESELGRTTDPVRMYMRE
HHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MGTVELLTREGEIVIAKRIEEGINTVQSSVAEYPQAIAMILEQFDQFEADELRLSDIISG
CCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
FVNPDEDVAPTATHIGSELSEEELEDEDDEEDDDDTEDEEEGNKGPDPEEARERFGQLRT
CCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHH
AYESALKIIEAKGREHPESVKALFEIGEIFKEFRLVPKQFDRLVKSMRTMMDRVRVQERL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKLCVEQAKMPKKNFVKLFTGNETNLDWFNTEKASTKPYAEGLRMVEDDVIRCRTKLAA
HHHHHHHHHHCCHHHCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VEEETGLAISAIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQ
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRI
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHH
SRQMLQEMGREPSPEELAERMMMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDT
HHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCC
TLELPLDSATSESLKNATHEVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRER
CEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
IRQIEAKALRKLRHPSRSEILKSFLDE
HHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLSVRRCNSFPTWMISMDHTPQSQLKLLLAKGKEQGYLTYAEVNDHLPADMVDSDQIEDI
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHH
IQMINDMGIRVFEEAPDADDMMMSEDNTDEDAAEEAAAALATVESELGRTTDPVRMYMRE
HHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MGTVELLTREGEIVIAKRIEEGINTVQSSVAEYPQAIAMILEQFDQFEADELRLSDIISG
CCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
FVNPDEDVAPTATHIGSELSEEELEDEDDEEDDDDTEDEEEGNKGPDPEEARERFGQLRT
CCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHH
AYESALKIIEAKGREHPESVKALFEIGEIFKEFRLVPKQFDRLVKSMRTMMDRVRVQERL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKLCVEQAKMPKKNFVKLFTGNETNLDWFNTEKASTKPYAEGLRMVEDDVIRCRTKLAA
HHHHHHHHHHCCHHHCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VEEETGLAISAIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQ
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRI
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHH
SRQMLQEMGREPSPEELAERMMMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDT
HHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCC
TLELPLDSATSESLKNATHEVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRER
CEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
IRQIEAKALRKLRHPSRSEILKSFLDE
HHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11272828 [H]