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The map label for this gene is hyfR [H]

Identifier: 160874180

GI number: 160874180

Start: 1257667

End: 1259127

Strand: Reverse

Name: hyfR [H]

Synonym: Sbal195_1060

Alternate gene names: 160874180

Gene position: 1259127-1257667 (Counterclockwise)

Preceding gene: 160874182

Following gene: 160874179

Centisome position: 23.55

GC content: 48.12

Gene sequence:

>1461_bases
ATGACAAATAAGATTTCGGTCAATAATCAAAAATCAGTCGATAAGCATTTCTTGCCTGAAAATCAGTTACTGCTTAACGC
TGTCGGTGAAGGTATTTATGGATTTGATTTAAGCGGGAATGCGGTGTTTATCAATCCCGCGGCTGAACGCATGACAGGTT
GGAAAAATGAAGAGTTACTGGGCAAAAATATCCATAACTGTCATCATCACAGCCATGCCGATGGTAGCCATTATCCCCAA
GAAGATTGCCCAATTTACAACACATTAAAGGATGGCATTACCCGCGAAATCACCCATGATGTGTTCTGGCGTAAAGATGG
GAGTAGCTTTCCCGTGCATTACAGTTCTACGCCCGTTTACCGTGATAATGAACTCATTGGCGTAGTCGCGATATTTCGTG
ACATTAGTATCCAAAAGCAAACGGAGCAGTCATTACGTCAAGCGTTGGCGCAAGTGCAAGCGCTCTCGGAGCGACTTGCC
TCCGAGAATCATTACCTACAGGCAGAACTAGCTGACAAAACGGGTGATGTGGATATTTCGGGTGGCAGTAGCGTGATCCG
CCACATGATCCAGCAATTGCATATGGTAGCCAATACCGACAGTACTGTGCTCATTTGTGGTGAAAATGGCACAGGCAAAG
AACTTGTAGCCCGTAACTTACATCAACTCAGTCGCCGCAAAGATAAACCTATGATCAGTGTCAACTGCGCCGCGTTTTCA
GCATCCTTGTTAGAAAGTGAACTTTTTGGTCACGAGAAAGGCGCATTCACCGGTGCCACCAGTCGCCGTAAAGGCCGCTT
CGAACTTGCTCACCAAGGCACGCTGTTCCTCGATGAAGTGGCAGAACTCAGTTTAGAAGCCCAATCAAAACTACTGCGGG
TCATCCAAGAGCAATCCTTCGAACGCCTCGGAGGCAGCGAAACCATTCAAGTGGATATTCGCTTGGTCGCCGCCAGTCAT
CACGACTTACTCAAGCGGGTCGAGCAGGGATTATTTCGGATGGATCTCTATTACCGCTTGAATGTATTCCCTATTCAAGT
GCCACCACTGCGCGCGAGGATCGAAGATATCCCCGAACTCGTCAGCCATATACTGCACAGTTTGAATCGTAAGTTAGGCA
AAAAAATCCGCGGTGTGAGCCAAAAAGGGCTGCAAAAGCTCATGGCTTATACATGGCCGGGCAATGTGCGCGAATTGCAA
AACATCCTCGAGCGCGAAGCCATTCTATCCCAAGGCGATATTCTGCAGATCCATGCGATGCCGAGCAACAACCCCTTAGC
TCAAGCCCAAGGCCAAACCTTAGCAGAAGCGGAAGCCTTGCATATAGAACAGACTCTTAAACGCTTAAACTGGCGAATAT
CCGGTCCACTCGGCGCGGCGAATGTCTTAGGCGTTCCGGCAAGTACACTGAGATCGCGGATGAAAAAACTGGGGATCCAA
CGTCAATCGAGTGAAATATAA

Upstream 100 bases:

>100_bases
AAAGTGGGAAATACTCACTAACTCAATCAAAGACACGATATATCGCGACATGCAACTTAAGGTATACTCGGGCAATACCA
GCCAAGCTTGAGTACCGCAG

Downstream 100 bases:

>100_bases
CTCTAACCACACAAGCTAAAAACGCGATATATCGCCAATGTGTGATATATCGTGACTACCACTTAGCTCGCATCATAAAA
TAACCATATATATCATAAGG

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTNKISVNNQKSVDKHFLPENQLLLNAVGEGIYGFDLSGNAVFINPAAERMTGWKNEELLGKNIHNCHHHSHADGSHYPQ
EDCPIYNTLKDGITREITHDVFWRKDGSSFPVHYSSTPVYRDNELIGVVAIFRDISIQKQTEQSLRQALAQVQALSERLA
SENHYLQAELADKTGDVDISGGSSVIRHMIQQLHMVANTDSTVLICGENGTGKELVARNLHQLSRRKDKPMISVNCAAFS
ASLLESELFGHEKGAFTGATSRRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQSFERLGGSETIQVDIRLVAASH
HDLLKRVEQGLFRMDLYYRLNVFPIQVPPLRARIEDIPELVSHILHSLNRKLGKKIRGVSQKGLQKLMAYTWPGNVRELQ
NILEREAILSQGDILQIHAMPSNNPLAQAQGQTLAEAEALHIEQTLKRLNWRISGPLGAANVLGVPASTLRSRMKKLGIQ
RQSSEI

Sequences:

>Translated_486_residues
MTNKISVNNQKSVDKHFLPENQLLLNAVGEGIYGFDLSGNAVFINPAAERMTGWKNEELLGKNIHNCHHHSHADGSHYPQ
EDCPIYNTLKDGITREITHDVFWRKDGSSFPVHYSSTPVYRDNELIGVVAIFRDISIQKQTEQSLRQALAQVQALSERLA
SENHYLQAELADKTGDVDISGGSSVIRHMIQQLHMVANTDSTVLICGENGTGKELVARNLHQLSRRKDKPMISVNCAAFS
ASLLESELFGHEKGAFTGATSRRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQSFERLGGSETIQVDIRLVAASH
HDLLKRVEQGLFRMDLYYRLNVFPIQVPPLRARIEDIPELVSHILHSLNRKLGKKIRGVSQKGLQKLMAYTWPGNVRELQ
NILEREAILSQGDILQIHAMPSNNPLAQAQGQTLAEAEALHIEQTLKRLNWRISGPLGAANVLGVPASTLRSRMKKLGIQ
RQSSEI
>Mature_485_residues
TNKISVNNQKSVDKHFLPENQLLLNAVGEGIYGFDLSGNAVFINPAAERMTGWKNEELLGKNIHNCHHHSHADGSHYPQE
DCPIYNTLKDGITREITHDVFWRKDGSSFPVHYSSTPVYRDNELIGVVAIFRDISIQKQTEQSLRQALAQVQALSERLAS
ENHYLQAELADKTGDVDISGGSSVIRHMIQQLHMVANTDSTVLICGENGTGKELVARNLHQLSRRKDKPMISVNCAAFSA
SLLESELFGHEKGAFTGATSRRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQSFERLGGSETIQVDIRLVAASHH
DLLKRVEQGLFRMDLYYRLNVFPIQVPPLRARIEDIPELVSHILHSLNRKLGKKIRGVSQKGLQKLMAYTWPGNVRELQN
ILEREAILSQGDILQIHAMPSNNPLAQAQGQTLAEAEALHIEQTLKRLNWRISGPLGAANVLGVPASTLRSRMKKLGIQR
QSSEI

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=342, Percent_Identity=49.4152046783626, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1789087, Length=342, Percent_Identity=48.5380116959064, Blast_Score=315, Evalue=5e-87,
Organism=Escherichia coli, GI1790437, Length=329, Percent_Identity=42.5531914893617, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1788550, Length=295, Percent_Identity=43.728813559322, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=40.0630914826498, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788905, Length=233, Percent_Identity=44.206008583691, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1790299, Length=245, Percent_Identity=44.4897959183673, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1786524, Length=322, Percent_Identity=39.4409937888199, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=46.3519313304721, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=46.7248908296943, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787583, Length=323, Percent_Identity=31.8885448916409, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=33.003300330033, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789828, Length=337, Percent_Identity=29.673590504451, Blast_Score=135, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54330; Mature: 54198

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKISVNNQKSVDKHFLPENQLLLNAVGEGIYGFDLSGNAVFINPAAERMTGWKNEELL
CCCCCCCCCCCCHHHHCCCCCHHHHHHHCCCEEEEECCCCEEEECCHHHHHCCCCCHHHH
GKNIHNCHHHSHADGSHYPQEDCPIYNTLKDGITREITHDVFWRKDGSSFPVHYSSTPVY
HCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
RDNELIGVVAIFRDISIQKQTEQSLRQALAQVQALSERLASENHYLQAELADKTGDVDIS
CCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEC
GGSSVIRHMIQQLHMVANTDSTVLICGENGTGKELVARNLHQLSRRKDKPMISVNCAAFS
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHH
ASLLESELFGHEKGAFTGATSRRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQSF
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERLGGSETIQVDIRLVAASHHDLLKRVEQGLFRMDLYYRLNVFPIQVPPLRARIEDIPEL
HHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHEEEEEEEEEEEEECCCHHHHHHHHHHH
VSHILHSLNRKLGKKIRGVSQKGLQKLMAYTWPGNVRELQNILEREAILSQGDILQIHAM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEC
PSNNPLAQAQGQTLAEAEALHIEQTLKRLNWRISGPLGAANVLGVPASTLRSRMKKLGIQ
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCHHCCCCHHHHHHHHHHHCCC
RQSSEI
CCCCCC
>Mature Secondary Structure 
TNKISVNNQKSVDKHFLPENQLLLNAVGEGIYGFDLSGNAVFINPAAERMTGWKNEELL
CCCCCCCCCCCHHHHCCCCCHHHHHHHCCCEEEEECCCCEEEECCHHHHHCCCCCHHHH
GKNIHNCHHHSHADGSHYPQEDCPIYNTLKDGITREITHDVFWRKDGSSFPVHYSSTPVY
HCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
RDNELIGVVAIFRDISIQKQTEQSLRQALAQVQALSERLASENHYLQAELADKTGDVDIS
CCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEC
GGSSVIRHMIQQLHMVANTDSTVLICGENGTGKELVARNLHQLSRRKDKPMISVNCAAFS
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHH
ASLLESELFGHEKGAFTGATSRRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQSF
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERLGGSETIQVDIRLVAASHHDLLKRVEQGLFRMDLYYRLNVFPIQVPPLRARIEDIPEL
HHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHEEEEEEEEEEEEECCCHHHHHHHHHHH
VSHILHSLNRKLGKKIRGVSQKGLQKLMAYTWPGNVRELQNILEREAILSQGDILQIHAM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEC
PSNNPLAQAQGQTLAEAEALHIEQTLKRLNWRISGPLGAANVLGVPASTLRSRMKKLGIQ
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCHHCCCCHHHHHHHHHHHCCC
RQSSEI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]