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The map label for this gene is chaA [H]

Identifier: 160874114

GI number: 160874114

Start: 1178450

End: 1179535

Strand: Reverse

Name: chaA [H]

Synonym: Sbal195_0994

Alternate gene names: 160874114

Gene position: 1179535-1178450 (Counterclockwise)

Preceding gene: 160874115

Following gene: 160874108

Centisome position: 22.06

GC content: 48.25

Gene sequence:

>1086_bases
ATGAAAGACGAGTTCGCCCTGCTACTGGGAATACTGACCTTAGTATTCTTTAAAACCGGTGGCGATAGCCTACTCGCCGA
TGGCACTGCGCTTTCGACCTATATTCCCGTATCACTCGCCCTATTAGGCGTTGTCACTTGGGCCATCTTCTCAGTGGTGC
GCCACTCTGATGCGCTAGCGATTAAGCTTGGCGATCCCTATGGCACTTTAATCTTAACCCTGTCGGTAATATCACTCGAA
GTGGTGATGATCTCTTCTGTGATGCTGACAGGGGAACCTAACCCTGTGATGGCGCGCGACACTATGTTCGCCGTGGTCAT
GTTGATTATCAACGGCTTAGTGGGATTCACCTTATTACTTGGCGGCTGGCGTTACCACACCCAGCTTTTTAATTTAGATG
GCGTAAAATCCTATTTGGTCGTCATTATCCCCCTCGCCTTACTCTGTTTAGTACTGCCCAATTTTACCGAAGGCGGCACT
ATCGGCAGCATGTCTAAAGCCATGTCTTGGATGCTGATCATTATTTCGATTCTGCTCTATGGCGTATTTTTGGTAGTACA
AACCAAGAGCCACAGTCATTTCTTTGTCGATGCCGATCATGAAGACCACGAGGAACACCACGGCATATTACGCAGTAATG
CCTATCATACTGTGCTATTGATTGGTTACTTGCTTGTGGTGATCTTATTGGCAAAAACCTTAGCCATGCCAATCAATTAC
AGTGTTGCGACCTTAGGCGCACCCCAGGCATTAGGTGGCTTTATCGTGGCCTGTTTGATCTTGTCACCCGAAGCGGTGGG
CGGATTTAAGGCGGCGTTAAACAATCAATTACAGCGGGCGATGAATCTGTATTTCGGCTCAGTACTCGCCACAATTGCCC
TTACCGTTCCCGCAGTATTAATTATTGGCGGCGTGATAGGTCAAGAAGTGCATTTAGGCTTGTCAGCCGCCGACATGGTG
CTGTTAATCACCAGTTTAATGGTCTGCAAGGTCAGCTTCAGCAGCGGTCGCACCAATGCCCTGCACGGCGCGACGCATAT
AGTGCTGTTTATCGTTTACTTGTTCTTAATGTTTGAACACGCCTAA

Upstream 100 bases:

>100_bases
ACCTCAAATCTAAAAGAAGTTTCATGCAAAGTGTGCCATAACAAGTGTCTATACTAACGGCCTTTTTTTCGCTTTGGACA
AACTGCCTATGAAGACATTT

Downstream 100 bases:

>100_bases
ATCTTCTTAAATCCAAAGCCGAGCGCGGCTTATTTGCGTCTCGGCTTACGTTTTTTAGCTGGACTATGGCCCTCAATCTT
CCGAGCTCTCTGAGCACCTC

Product: sodium/calcium exchanger membrane region

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKDEFALLLGILTLVFFKTGGDSLLADGTALSTYIPVSLALLGVVTWAIFSVVRHSDALAIKLGDPYGTLILTLSVISLE
VVMISSVMLTGEPNPVMARDTMFAVVMLIINGLVGFTLLLGGWRYHTQLFNLDGVKSYLVVIIPLALLCLVLPNFTEGGT
IGSMSKAMSWMLIIISILLYGVFLVVQTKSHSHFFVDADHEDHEEHHGILRSNAYHTVLLIGYLLVVILLAKTLAMPINY
SVATLGAPQALGGFIVACLILSPEAVGGFKAALNNQLQRAMNLYFGSVLATIALTVPAVLIIGGVIGQEVHLGLSAADMV
LLITSLMVCKVSFSSGRTNALHGATHIVLFIVYLFLMFEHA

Sequences:

>Translated_361_residues
MKDEFALLLGILTLVFFKTGGDSLLADGTALSTYIPVSLALLGVVTWAIFSVVRHSDALAIKLGDPYGTLILTLSVISLE
VVMISSVMLTGEPNPVMARDTMFAVVMLIINGLVGFTLLLGGWRYHTQLFNLDGVKSYLVVIIPLALLCLVLPNFTEGGT
IGSMSKAMSWMLIIISILLYGVFLVVQTKSHSHFFVDADHEDHEEHHGILRSNAYHTVLLIGYLLVVILLAKTLAMPINY
SVATLGAPQALGGFIVACLILSPEAVGGFKAALNNQLQRAMNLYFGSVLATIALTVPAVLIIGGVIGQEVHLGLSAADMV
LLITSLMVCKVSFSSGRTNALHGATHIVLFIVYLFLMFEHA
>Mature_361_residues
MKDEFALLLGILTLVFFKTGGDSLLADGTALSTYIPVSLALLGVVTWAIFSVVRHSDALAIKLGDPYGTLILTLSVISLE
VVMISSVMLTGEPNPVMARDTMFAVVMLIINGLVGFTLLLGGWRYHTQLFNLDGVKSYLVVIIPLALLCLVLPNFTEGGT
IGSMSKAMSWMLIIISILLYGVFLVVQTKSHSHFFVDADHEDHEEHHGILRSNAYHTVLLIGYLLVVILLAKTLAMPINY
SVATLGAPQALGGFIVACLILSPEAVGGFKAALNNQLQRAMNLYFGSVLATIALTVPAVLIIGGVIGQEVHLGLSAADMV
LLITSLMVCKVSFSSGRTNALHGATHIVLFIVYLFLMFEHA

Specific function: According to PubMed:8496184 the natural activity of this protein is as a calcium/proton antiporter, but it can act as a weak sodium/proton antiporter. The presence of a preferred substrate and normal pH cytoplasmic values might prevent significant sodium/

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=332, Percent_Identity=47.5903614457831, Blast_Score=287, Evalue=7e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 38754; Mature: 38754

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEFALLLGILTLVFFKTGGDSLLADGTALSTYIPVSLALLGVVTWAIFSVVRHSDALA
CCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IKLGDPYGTLILTLSVISLEVVMISSVMLTGEPNPVMARDTMFAVVMLIINGLVGFTLLL
EEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GGWRYHTQLFNLDGVKSYLVVIIPLALLCLVLPNFTEGGTIGSMSKAMSWMLIIISILLY
CCHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GVFLVVQTKSHSHFFVDADHEDHEEHHGILRSNAYHTVLLIGYLLVVILLAKTLAMPINY
HHHHHEEECCCCEEEEECCCCCCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SVATLGAPQALGGFIVACLILSPEAVGGFKAALNNQLQRAMNLYFGSVLATIALTVPAVL
CEEECCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGGVIGQEVHLGLSAADMVLLITSLMVCKVSFSSGRTNALHGATHIVLFIVYLFLMFEH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
A
C
>Mature Secondary Structure
MKDEFALLLGILTLVFFKTGGDSLLADGTALSTYIPVSLALLGVVTWAIFSVVRHSDALA
CCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IKLGDPYGTLILTLSVISLEVVMISSVMLTGEPNPVMARDTMFAVVMLIINGLVGFTLLL
EEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GGWRYHTQLFNLDGVKSYLVVIIPLALLCLVLPNFTEGGTIGSMSKAMSWMLIIISILLY
CCHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GVFLVVQTKSHSHFFVDADHEDHEEHHGILRSNAYHTVLLIGYLLVVILLAKTLAMPINY
HHHHHEEECCCCEEEEECCCCCCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SVATLGAPQALGGFIVACLILSPEAVGGFKAALNNQLQRAMNLYFGSVLATIALTVPAVL
CEEECCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGGVIGQEVHLGLSAADMVLLITSLMVCKVSFSSGRTNALHGATHIVLFIVYLFLMFEH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8496184; 8905232; 9278503 [H]