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The map label for this gene is selB [H]

Identifier: 160873236

GI number: 160873236

Start: 131858

End: 133903

Strand: Direct

Name: selB [H]

Synonym: Sbal195_0110

Alternate gene names: 160873236

Gene position: 131858-133903 (Clockwise)

Preceding gene: 160873235

Following gene: 160873237

Centisome position: 2.47

GC content: 51.86

Gene sequence:

>2046_bases
GTGATCATTGTTACTGCGGGTCATGTCGATCACGGCAAGAGTACGCTTATTCGCGCCTTGACTGGGATGAATACCGATCG
TCTGCCGGAGGAGAAGCGCCGCGGTATGACGATCGATCTCGGCTATGCGTTTATGCCGCTTAGGGATGGTTCGCGCCTCG
CTTTTATTGATGTGCCTGGGCATGAAAAATTTATCAACAACATGCTGGTGGGCGTGAGTCATGCTCGCCATGCCTTACTC
GTGCTGGCCTGTGATGATGGCGTTATGCCGCAAACCCGCGAACATCTGCAAATCCTAGCGCTCTTACCGCTTCACAGTTT
GACCTTAGTGCTGACTAAGCGGGATCTGGTTGATAGTGATATGGCTGAGCAGCTTGTCGCCGATGGACTCACTTTGCTGG
CCGAGTATGGCATTGAGGCCAGTGGTTTTTTTCAGGTGTCGGCAAGTGAAACTGCCGACTTAGCATCCAATGGTGTGGCC
GCACTTAAGGCGCATATTCTTGATTTAGCTGAGCAGGAGTCTAAGCGGGCTGAGGATGCCAGCAGTTTTAGGATGACGCT
CGATCGCGCCTTTAGTGTGAAGGGTGCAGGTTGTGTGGTTACGGGCACAGTGATCAGCGGCAATGTGTGCGTGGGTGATA
GTCTTTTTAGCTCAGGACAAAAGGCCAAGTTGAGAGTACGAGGCATCCATTGTCAAGGACAAGAAGCCCAAAGGGCCGTC
ACGGGGACACGTGTAGCACTCAATTTAACCGGAGTTGATAACCATAGGTCGCCCGAACGCGGCGATTGGCTAAGCAGCTT
GCCTCAAGCTGAGCACTGTAGCCGCCCTGTGGTGCATATTCGAGTCTTCGATTTATTAGGTATTGAGCCCTTGGTACATT
GGCAAAATGTTCACTGTCACCATGGCGCTGATCATACTTTAGGGCGAGTTTCGCTGTTAAATGAAGCTGATGCTGAAGGT
TTTTATCTGGCGGAGTTGGTACTCGAAAAGCCGCTACTTTTATGCCAAGACGATCCCATCGTCCTGCGCCACATTGGCGG
TAAACAGACTTTAGGTGCAGGGCGAGTGTTGGCGCTTAAAGTCCCTAATCGAAAGAAGCGCACGCCTGAACGAGTCGAGA
GATTACAGCAAGTAGCGCGCTTGGATAATCCTGCCCATGTGGTGGCTTTACTCGGCCAAACTCAGGCGCTAGAACTTGCT
GAAATTGCGTGGCGTTGGCAATTAACCGACGAAGGGCTCGCTATGCTCTTAGCCCAAGCGGGTTTAGTCCGTCTTGGCGA
GTTTGCCGTGGCCACACGTTTACTTGAGCAAGAAAAACAACAGTTTATTACTTTACTGAGCGAGTTCCATCAACAACATC
CCGACCAACTTGGGCTTGGGCGTAATCGTCTGCAACGTATGAGCCACAGCATATTACCCAACGAGCTTGCCAATAAGGTG
CTCGATAGCCTCTGCCAAGATGGGCAGATGGTGCTGCGCGGTGCCTTGCTGCAGTTGGCGGACCACAGAATTGTCCTCGA
TAGCGAGGCGCAGCAGTGCTGGCAACGCTTGGCTCCTTGGTTGGCGCAGCAAACGTCCCCTGTGTGGGTGACTGAGATGG
GGGAATATCTCGAACTCGAGCCCGCCAAACTCAGATTGCTGTGCTTTCAACTGGTGCAACAGGGCTTTATTACCGCTGTG
GTGAAGGACAGATATCTGCTGAGTGAGCAGTTGTGCCACTACGCCAATCTGGTGCGTGAGCATGTGGACACCCACGGCAA
GCTCGAAACTGCTGAATTTAAAGACTTAATAGGGCTTGGTCGCAAGGTCAGCATTCAGCTGCTTGAGTTTTTTGACAGAA
GTGGTTTTACCCGTAGGAAATATCGCAGTAATAGCCGGGAAATTCGCGACGCGGAGCTTTTTTTCTCCCATGAGAATTGG
CGGAAGAACATAGGAGTCGAACCTACCCAGGACTGCTGGCAGCCCTACCCGGATTTGAAATCCGGACGCCTCACCGGAGA
CGACGTTCTTCCAATAGAAGACTTAGGGATAGTAGAAAATGCTTAA

Upstream 100 bases:

>100_bases
TCATAGGTCGCATGAGTAACGATCAACTCAGATTGGATTTACGCGGTCTTGATAGCGAAGCCGAGCTACTCGCCGAGCTG
AGCATATTAGGTGTGCAGTT

Downstream 100 bases:

>100_bases
AGATGATTCAAGGCGATTCGTATTTAGTTTATGGGTTTGTGATACAAGTCTTAAATTGTCATCAAACTAATTGTTTTTAT
TTGATTTTATAGTTTTAGAG

Product: selenocysteine-specific translation elongation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MIIVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGSRLAFIDVPGHEKFINNMLVGVSHARHALL
VLACDDGVMPQTREHLQILALLPLHSLTLVLTKRDLVDSDMAEQLVADGLTLLAEYGIEASGFFQVSASETADLASNGVA
ALKAHILDLAEQESKRAEDASSFRMTLDRAFSVKGAGCVVTGTVISGNVCVGDSLFSSGQKAKLRVRGIHCQGQEAQRAV
TGTRVALNLTGVDNHRSPERGDWLSSLPQAEHCSRPVVHIRVFDLLGIEPLVHWQNVHCHHGADHTLGRVSLLNEADAEG
FYLAELVLEKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRTPERVERLQQVARLDNPAHVVALLGQTQALELA
EIAWRWQLTDEGLAMLLAQAGLVRLGEFAVATRLLEQEKQQFITLLSEFHQQHPDQLGLGRNRLQRMSHSILPNELANKV
LDSLCQDGQMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLAQQTSPVWVTEMGEYLELEPAKLRLLCFQLVQQGFITAV
VKDRYLLSEQLCHYANLVREHVDTHGKLETAEFKDLIGLGRKVSIQLLEFFDRSGFTRRKYRSNSREIRDAELFFSHENW
RKNIGVEPTQDCWQPYPDLKSGRLTGDDVLPIEDLGIVENA

Sequences:

>Translated_681_residues
MIIVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGSRLAFIDVPGHEKFINNMLVGVSHARHALL
VLACDDGVMPQTREHLQILALLPLHSLTLVLTKRDLVDSDMAEQLVADGLTLLAEYGIEASGFFQVSASETADLASNGVA
ALKAHILDLAEQESKRAEDASSFRMTLDRAFSVKGAGCVVTGTVISGNVCVGDSLFSSGQKAKLRVRGIHCQGQEAQRAV
TGTRVALNLTGVDNHRSPERGDWLSSLPQAEHCSRPVVHIRVFDLLGIEPLVHWQNVHCHHGADHTLGRVSLLNEADAEG
FYLAELVLEKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRTPERVERLQQVARLDNPAHVVALLGQTQALELA
EIAWRWQLTDEGLAMLLAQAGLVRLGEFAVATRLLEQEKQQFITLLSEFHQQHPDQLGLGRNRLQRMSHSILPNELANKV
LDSLCQDGQMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLAQQTSPVWVTEMGEYLELEPAKLRLLCFQLVQQGFITAV
VKDRYLLSEQLCHYANLVREHVDTHGKLETAEFKDLIGLGRKVSIQLLEFFDRSGFTRRKYRSNSREIRDAELFFSHENW
RKNIGVEPTQDCWQPYPDLKSGRLTGDDVLPIEDLGIVENA
>Mature_681_residues
MIIVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGSRLAFIDVPGHEKFINNMLVGVSHARHALL
VLACDDGVMPQTREHLQILALLPLHSLTLVLTKRDLVDSDMAEQLVADGLTLLAEYGIEASGFFQVSASETADLASNGVA
ALKAHILDLAEQESKRAEDASSFRMTLDRAFSVKGAGCVVTGTVISGNVCVGDSLFSSGQKAKLRVRGIHCQGQEAQRAV
TGTRVALNLTGVDNHRSPERGDWLSSLPQAEHCSRPVVHIRVFDLLGIEPLVHWQNVHCHHGADHTLGRVSLLNEADAEG
FYLAELVLEKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRTPERVERLQQVARLDNPAHVVALLGQTQALELA
EIAWRWQLTDEGLAMLLAQAGLVRLGEFAVATRLLEQEKQQFITLLSEFHQQHPDQLGLGRNRLQRMSHSILPNELANKV
LDSLCQDGQMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLAQQTSPVWVTEMGEYLELEPAKLRLLCFQLVQQGFITAV
VKDRYLLSEQLCHYANLVREHVDTHGKLETAEFKDLIGLGRKVSIQLLEFFDRSGFTRRKYRSNSREIRDAELFFSHENW
RKNIGVEPTQDCWQPYPDLKSGRLTGDDVLPIEDLGIVENA

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=268, Percent_Identity=30.5970149253731, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI54607086, Length=350, Percent_Identity=26.8571428571429, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI53729339, Length=230, Percent_Identity=28.695652173913, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI53729337, Length=230, Percent_Identity=28.695652173913, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI2367247, Length=634, Percent_Identity=42.7444794952681, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1790412, Length=289, Percent_Identity=33.2179930795848, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1789737, Length=289, Percent_Identity=33.2179930795848, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1789108, Length=421, Percent_Identity=26.1282660332542, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1789559, Length=354, Percent_Identity=27.683615819209, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI25141371, Length=277, Percent_Identity=28.8808664259928, Blast_Score=106, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17506081, Length=270, Percent_Identity=28.8888888888889, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=280, Percent_Identity=29.6428571428571, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=281, Percent_Identity=31.3167259786477, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6325337, Length=310, Percent_Identity=24.8387096774194, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319594, Length=310, Percent_Identity=24.8387096774194, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921738, Length=271, Percent_Identity=30.9963099630996, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281363316, Length=227, Percent_Identity=31.2775330396476, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17864358, Length=227, Percent_Identity=31.2775330396476, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19922690, Length=333, Percent_Identity=27.027027027027, Blast_Score=92, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550900, Length=300, Percent_Identity=26.3333333333333, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28572034, Length=97, Percent_Identity=37.1134020618557, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 75903; Mature: 75903

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGSRLAFIDVPG
CEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEEEEECCCCEEEEEECCC
HEKFINNMLVGVSHARHALLVLACDDGVMPQTREHLQILALLPLHSLTLVLTKRDLVDSD
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEHHHHHCHH
MAEQLVADGLTLLAEYGIEASGFFQVSASETADLASNGVAALKAHILDLAEQESKRAEDA
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SSFRMTLDRAFSVKGAGCVVTGTVISGNVCVGDSLFSSGQKAKLRVRGIHCQGQEAQRAV
HHHHHHHHHHHCCCCCCEEEEEEEEECCEEECHHHHCCCCCEEEEEEEEECCCHHHHHHH
TGTRVALNLTGVDNHRSPERGDWLSSLPQAEHCSRPVVHIRVFDLLGIEPLVHWQNVHCH
CCCEEEEEEECCCCCCCCCCCCHHHCCCCHHHCCCCEEEEEEEHHHCCCHHHHHCCCCCC
HGADHTLGRVSLLNEADAEGFYLAELVLEKPLLLCQDDPIVLRHIGGKQTLGAGRVLALK
CCCCCCCHHHEECCCCCCCCHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCCEEEEE
VPNRKKRTPERVERLQQVARLDNPAHVVALLGQTQALELAEIAWRWQLTDEGLAMLLAQA
CCCCCCCCHHHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHHEEEEECHHHHHHHHHHH
GLVRLGEFAVATRLLEQEKQQFITLLSEFHQQHPDQLGLGRNRLQRMSHSILPNELANKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LDSLCQDGQMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLAQQTSPVWVTEMGEYLELE
HHHHHCCCHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCEEHCCCCCHHCCC
PAKLRLLCFQLVQQGFITAVVKDRYLLSEQLCHYANLVREHVDTHGKLETAEFKDLIGLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCC
RKVSIQLLEFFDRSGFTRRKYRSNSREIRDAELFFSHENWRKNIGVEPTQDCWQPYPDLK
CHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHEEEECCHHHHCCCCCCCHHHCCCCCCCC
SGRLTGDDVLPIEDLGIVENA
CCCCCCCCCCCHHCCCCCCCC
>Mature Secondary Structure
MIIVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGSRLAFIDVPG
CEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEEEEECCCCEEEEEECCC
HEKFINNMLVGVSHARHALLVLACDDGVMPQTREHLQILALLPLHSLTLVLTKRDLVDSD
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEHHHHHCHH
MAEQLVADGLTLLAEYGIEASGFFQVSASETADLASNGVAALKAHILDLAEQESKRAEDA
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SSFRMTLDRAFSVKGAGCVVTGTVISGNVCVGDSLFSSGQKAKLRVRGIHCQGQEAQRAV
HHHHHHHHHHHCCCCCCEEEEEEEEECCEEECHHHHCCCCCEEEEEEEEECCCHHHHHHH
TGTRVALNLTGVDNHRSPERGDWLSSLPQAEHCSRPVVHIRVFDLLGIEPLVHWQNVHCH
CCCEEEEEEECCCCCCCCCCCCHHHCCCCHHHCCCCEEEEEEEHHHCCCHHHHHCCCCCC
HGADHTLGRVSLLNEADAEGFYLAELVLEKPLLLCQDDPIVLRHIGGKQTLGAGRVLALK
CCCCCCCHHHEECCCCCCCCHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCCEEEEE
VPNRKKRTPERVERLQQVARLDNPAHVVALLGQTQALELAEIAWRWQLTDEGLAMLLAQA
CCCCCCCCHHHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHHEEEEECHHHHHHHHHHH
GLVRLGEFAVATRLLEQEKQQFITLLSEFHQQHPDQLGLGRNRLQRMSHSILPNELANKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LDSLCQDGQMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLAQQTSPVWVTEMGEYLELE
HHHHHCCCHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCEEHCCCCCHHCCC
PAKLRLLCFQLVQQGFITAVVKDRYLLSEQLCHYANLVREHVDTHGKLETAEFKDLIGLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCC
RKVSIQLLEFFDRSGFTRRKYRSNSREIRDAELFFSHENWRKNIGVEPTQDCWQPYPDLK
CHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHEEEECCHHHHCCCCCCCHHHCCCCCCCC
SGRLTGDDVLPIEDLGIVENA
CCCCCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]