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The map label for this gene is fdnG [H]

Identifier: 160873231

GI number: 160873231

Start: 125501

End: 127885

Strand: Direct

Name: fdnG [H]

Synonym: Sbal195_0105

Alternate gene names: 160873231

Gene position: 125501-127885 (Clockwise)

Preceding gene: 160873230

Following gene: 160873232

Centisome position: 2.35

GC content: 49.01

Gene sequence:

>2385_bases
ATGACCAACCACTGGATCGATATTAAAAATTCTAATTTGATCATTATCATGGGCGGTAATGCTGCAGAGGCTCACCCTGT
CGGATTCGGCTGGGTTACAGAAGCAATGCAACATAACAACGCTAAACTTTTGGTGGTCGATCCTCGCTTCACCCGTAGCG
CCGCTGTGGCCGATTATTATGCGCCGATCCGTTCGGGCACAGATATCGCCTTCCTGCTCGGGGTGATCCGCTATCTTATT
GAAACCAAACAAGTGAACTACGACTATGTGAAGGCTTATACCAATGCCAGTTACATAGTGCGCGAAGACTTCTCTTTTAG
TGAAGGTCTGTTCAGTGGTTTCGATGAAGAAACAGATAGCTACGATAAAGAGTCTTGGTACTACGAGTTAGACGAACAGG
GTTACGCGAAAGTCGATCCTAGCTTCGAGCATCCACGCTGCGTGTGGAACTTAATGAAGCAGCATGTAGATCGCTATGAC
TTTGAAACAGTAAGCAATATTACCGGTACGCCGATTCCGGACTATGAGATGGTTTGCCAGCAAATTGGCAGCACTCATAC
CCACGATAGAGCCGCCACTTTTATGTATGCCTTAGGTTGGACTCACCATACTAAAGGGGCGCAGAACATTCGCTCTATGG
CTATGGTGCAGTTGCTGCTGGGTAACATTGGGGTGTTGGGCGGTGGCGTTAACGCACTGCGTGGTCACTCTAACGTGCAA
GGCGCGACTGATATGGGTTTATTGTGCCAAAGCTTGCCTGGTTACTTGAAGTTACCGAGTGAAAAAGACACTGATCTTAA
GACCTATCTCGATCGTTACACGCCTGTCGCCTTGCGTCCGGGTCAAACCAACTATTGGCAAAATTATCCTAAATTCTTTA
TTTCACAAATGAAGAGCTTCTGGGGCGATAACGCCACGGTTGAAAACGACTTTGGTTATGACTGGGTGCCTAAGTGGGAT
AAACAGTACGACTTTACTAAGCACTTAGACATGGCTTTCCACGGTGAAGTGAATGGCTACATTATCCAAGGTGTTAACGC
CATTAATTCTATGCCGAACCGCAATAAGGTACTCAAAGCCTTGAGTAATCTTAAGTACATGGTGGTGCTGGATGCCTTGG
CAACGGAAACCGCAACCTTCTGGCAAAATGCCGATGGCTTCAACGAGGTGAATCCCGCTGAAATCATGACTGAAGTGTTC
CGCTTACCGACGACGTGTTTTGCCGAAGAAGAAGGTTCCATCGTCAACTCTGGACGTTGGATGCAGTGGCATTGGAAGGG
CGCTAACCCGCCGGGTGAAGCCAAGCCAGATGCTGAGATCCTCTCCGGTGTCTTAATGGCCATGCGTGAACTGTATAAGC
AAGAGGGTGGTAAATTGCCTGAGCCAGTGCAAGCGATAAGTTGGGATTACCACAATCCTTACTCGCCGCATGCCGAAGAA
GTTGCTCGTGAGCTTAACGGTAAGGATCTCGTTACTCAGCGTCAGCTCAGTAGCTTCTCTGAACTCAAAGCTGATGGTAG
TACTGCTAGCGCCTGTTGGATTTATGCAGGTTCTTGGACCGAAGAGGGCAACCTGATGGCTCGCCGCGACAACCATGATC
CTTCGGGTAAAGGTGTCACTCCGGGCTGGGCATTTGCTTGGCCAGCCAACCGTCGCGTGCTGTATAACCGCGCTTCTTGC
GATATCAACGGCAAACCTTGGGATCCTAAACGTACTATCGTCGAGTGGGTTGATGGTAAGTGGCATGGTATCGATGTGGG
CGACTTCAATATGAAGTTAACACCACAGGAATCCGCAGGTCCCTTCATCATGCAACCCGAAGGTGTGGGTCGTTTCTTTG
CCCTTAAGTTGTTGGCCGAAGGTCCGTTCCCTGAGCACTACGAGCCGATGGAGTCGCCTATTGGGTTGAACCCGTTACAC
AAGGTCACCAGTAACCCTGCGGTACGTATGTTACCGGGTGTGAAGGAAACCTTAGGTTCCCATAAGGACTTCCCTTATGT
GGCAACTACTTACTGTGTAACTGAACACTTTAACTTCTGGTCTACGCATGCTCGTTTAGCTGCGATTTCTATGCCTGAAA
CCTTCGTCGAAATCGATGAAATGTTAGCGGCAGAAAAAGGTATCGCCAATGGGGATTGGGTCACTGTCAGCTCTAAACGC
GGCAGCATTGAAACCAAAGCCTTAGTGACTAAACGGCTACAGCCGTTAAAGGTAAATGGCCAGTTAGTCCATACCGTCGG
GTTACCCCGTCACGGCAGCCATAATGCACTCACTCGTAAGAGTTACTCCTGTAACGTACTCACCACGGAAGTGGGGGATG
CAAATACACAGGTGCCTGAGTTCAAAGCATTTTTGGTTAACATCACTAAAGCGGAGGGGATCTGA

Upstream 100 bases:

>100_bases
ATTTGCACGGGCACTGGGCCTAGTTGCAATCGATACTATTGCGCGTAACTGACACTCTCCAACGGTAGCAAGTCTTGCTC
CGACATTTGGACGTGGTGCC

Downstream 100 bases:

>100_bases
TTATGGCATCACAAGACATTATCCGCCGCTCCGGTACTTCAGCGGTGACGCCTGCGGCAAAGGGGCGTAATTCGTCAGAA
AAGGTCGCCAAGCTGTTCGA

Product: formate dehydrogenase subunit alpha

Products: NA

Alternate protein names: Anaerobic formate dehydrogenase major subunit; Formate dehydrogenase-N subunit alpha; FDH-N subunit alpha [H]

Number of amino acids: Translated: 794; Mature: 793

Protein sequence:

>794_residues
MTNHWIDIKNSNLIIIMGGNAAEAHPVGFGWVTEAMQHNNAKLLVVDPRFTRSAAVADYYAPIRSGTDIAFLLGVIRYLI
ETKQVNYDYVKAYTNASYIVREDFSFSEGLFSGFDEETDSYDKESWYYELDEQGYAKVDPSFEHPRCVWNLMKQHVDRYD
FETVSNITGTPIPDYEMVCQQIGSTHTHDRAATFMYALGWTHHTKGAQNIRSMAMVQLLLGNIGVLGGGVNALRGHSNVQ
GATDMGLLCQSLPGYLKLPSEKDTDLKTYLDRYTPVALRPGQTNYWQNYPKFFISQMKSFWGDNATVENDFGYDWVPKWD
KQYDFTKHLDMAFHGEVNGYIIQGVNAINSMPNRNKVLKALSNLKYMVVLDALATETATFWQNADGFNEVNPAEIMTEVF
RLPTTCFAEEEGSIVNSGRWMQWHWKGANPPGEAKPDAEILSGVLMAMRELYKQEGGKLPEPVQAISWDYHNPYSPHAEE
VARELNGKDLVTQRQLSSFSELKADGSTASACWIYAGSWTEEGNLMARRDNHDPSGKGVTPGWAFAWPANRRVLYNRASC
DINGKPWDPKRTIVEWVDGKWHGIDVGDFNMKLTPQESAGPFIMQPEGVGRFFALKLLAEGPFPEHYEPMESPIGLNPLH
KVTSNPAVRMLPGVKETLGSHKDFPYVATTYCVTEHFNFWSTHARLAAISMPETFVEIDEMLAAEKGIANGDWVTVSSKR
GSIETKALVTKRLQPLKVNGQLVHTVGLPRHGSHNALTRKSYSCNVLTTEVGDANTQVPEFKAFLVNITKAEGI

Sequences:

>Translated_794_residues
MTNHWIDIKNSNLIIIMGGNAAEAHPVGFGWVTEAMQHNNAKLLVVDPRFTRSAAVADYYAPIRSGTDIAFLLGVIRYLI
ETKQVNYDYVKAYTNASYIVREDFSFSEGLFSGFDEETDSYDKESWYYELDEQGYAKVDPSFEHPRCVWNLMKQHVDRYD
FETVSNITGTPIPDYEMVCQQIGSTHTHDRAATFMYALGWTHHTKGAQNIRSMAMVQLLLGNIGVLGGGVNALRGHSNVQ
GATDMGLLCQSLPGYLKLPSEKDTDLKTYLDRYTPVALRPGQTNYWQNYPKFFISQMKSFWGDNATVENDFGYDWVPKWD
KQYDFTKHLDMAFHGEVNGYIIQGVNAINSMPNRNKVLKALSNLKYMVVLDALATETATFWQNADGFNEVNPAEIMTEVF
RLPTTCFAEEEGSIVNSGRWMQWHWKGANPPGEAKPDAEILSGVLMAMRELYKQEGGKLPEPVQAISWDYHNPYSPHAEE
VARELNGKDLVTQRQLSSFSELKADGSTASACWIYAGSWTEEGNLMARRDNHDPSGKGVTPGWAFAWPANRRVLYNRASC
DINGKPWDPKRTIVEWVDGKWHGIDVGDFNMKLTPQESAGPFIMQPEGVGRFFALKLLAEGPFPEHYEPMESPIGLNPLH
KVTSNPAVRMLPGVKETLGSHKDFPYVATTYCVTEHFNFWSTHARLAAISMPETFVEIDEMLAAEKGIANGDWVTVSSKR
GSIETKALVTKRLQPLKVNGQLVHTVGLPRHGSHNALTRKSYSCNVLTTEVGDANTQVPEFKAFLVNITKAEGI
>Mature_793_residues
TNHWIDIKNSNLIIIMGGNAAEAHPVGFGWVTEAMQHNNAKLLVVDPRFTRSAAVADYYAPIRSGTDIAFLLGVIRYLIE
TKQVNYDYVKAYTNASYIVREDFSFSEGLFSGFDEETDSYDKESWYYELDEQGYAKVDPSFEHPRCVWNLMKQHVDRYDF
ETVSNITGTPIPDYEMVCQQIGSTHTHDRAATFMYALGWTHHTKGAQNIRSMAMVQLLLGNIGVLGGGVNALRGHSNVQG
ATDMGLLCQSLPGYLKLPSEKDTDLKTYLDRYTPVALRPGQTNYWQNYPKFFISQMKSFWGDNATVENDFGYDWVPKWDK
QYDFTKHLDMAFHGEVNGYIIQGVNAINSMPNRNKVLKALSNLKYMVVLDALATETATFWQNADGFNEVNPAEIMTEVFR
LPTTCFAEEEGSIVNSGRWMQWHWKGANPPGEAKPDAEILSGVLMAMRELYKQEGGKLPEPVQAISWDYHNPYSPHAEEV
ARELNGKDLVTQRQLSSFSELKADGSTASACWIYAGSWTEEGNLMARRDNHDPSGKGVTPGWAFAWPANRRVLYNRASCD
INGKPWDPKRTIVEWVDGKWHGIDVGDFNMKLTPQESAGPFIMQPEGVGRFFALKLLAEGPFPEHYEPMESPIGLNPLHK
VTSNPAVRMLPGVKETLGSHKDFPYVATTYCVTEHFNFWSTHARLAAISMPETFVEIDEMLAAEKGIANGDWVTVSSKRG
SIETKALVTKRLQPLKVNGQLVHTVGLPRHGSHNALTRKSYSCNVLTTEVGDANTQVPEFKAFLVNITKAEGI

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868719, Length=804, Percent_Identity=61.81592039801, Blast_Score=1045, Evalue=0.0,
Organism=Escherichia coli, GI3868720, Length=804, Percent_Identity=59.9502487562189, Blast_Score=1024, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=450, Percent_Identity=24.6666666666667, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081797, Length=239, Percent_Identity=28.4518828451883, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1787870, Length=235, Percent_Identity=28.0851063829787, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006443
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.2.1.2 [H]

Molecular weight: Translated: 89147; Mature: 89016

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHWIDIKNSNLIIIMGGNAAEAHPVGFGWVTEAMQHNNAKLLVVDPRFTRSAAVADYY
CCCCEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
APIRSGTDIAFLLGVIRYLIETKQVNYDYVKAYTNASYIVREDFSFSEGLFSGFDEETDS
HHHCCCCHHHHHHHHHHHHHHHHCCCHHHHEEECCCEEEEECCCCHHHHHHCCCCCCCCC
YDKESWYYELDEQGYAKVDPSFEHPRCVWNLMKQHVDRYDFETVSNITGTPIPDYEMVCQ
CCCCCCEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
QIGSTHTHDRAATFMYALGWTHHTKGAQNIRSMAMVQLLLGNIGVLGGGVNALRGHSNVQ
HHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCCCCC
GATDMGLLCQSLPGYLKLPSEKDTDLKTYLDRYTPVALRPGQTNYWQNYPKFFISQMKSF
CCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHH
WGDNATVENDFGYDWVPKWDKQYDFTKHLDMAFHGEVNGYIIQGVNAINSMPNRNKVLKA
HCCCCEEECCCCCCCCCCCCCCCCHHHHCCEEEECCCCCEEEECCHHHHCCCCHHHHHHH
LSNLKYMVVLDALATETATFWQNADGFNEVNPAEIMTEVFRLPTTCFAEEEGSIVNSGRW
HHCCEEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCEECCCCE
MQWHWKGANPPGEAKPDAEILSGVLMAMRELYKQEGGKLPEPVQAISWDYHNPYSPHAEE
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHH
VARELNGKDLVTQRQLSSFSELKADGSTASACWIYAGSWTEEGNLMARRDNHDPSGKGVT
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCC
PGWAFAWPANRRVLYNRASCDINGKPWDPKRTIVEWVDGKWHGIDVGDFNMKLTPQESAG
CCEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCEEECCCCCCC
PFIMQPEGVGRFFALKLLAEGPFPEHYEPMESPIGLNPLHKVTSNPAVRMLPGVKETLGS
CEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEECCCHHHHHCC
HKDFPYVATTYCVTEHFNFWSTHARLAAISMPETFVEIDEMLAAEKGIANGDWVTVSSKR
CCCCCEEEEEEEHHHCCCHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
GSIETKALVTKRLQPLKVNGQLVHTVGLPRHGSHNALTRKSYSCNVLTTEVGDANTQVPE
CCCHHHHHHHHCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
FKAFLVNITKAEGI
CEEEEEEEECCCCC
>Mature Secondary Structure 
TNHWIDIKNSNLIIIMGGNAAEAHPVGFGWVTEAMQHNNAKLLVVDPRFTRSAAVADYY
CCCEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
APIRSGTDIAFLLGVIRYLIETKQVNYDYVKAYTNASYIVREDFSFSEGLFSGFDEETDS
HHHCCCCHHHHHHHHHHHHHHHHCCCHHHHEEECCCEEEEECCCCHHHHHHCCCCCCCCC
YDKESWYYELDEQGYAKVDPSFEHPRCVWNLMKQHVDRYDFETVSNITGTPIPDYEMVCQ
CCCCCCEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
QIGSTHTHDRAATFMYALGWTHHTKGAQNIRSMAMVQLLLGNIGVLGGGVNALRGHSNVQ
HHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCCCCC
GATDMGLLCQSLPGYLKLPSEKDTDLKTYLDRYTPVALRPGQTNYWQNYPKFFISQMKSF
CCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHH
WGDNATVENDFGYDWVPKWDKQYDFTKHLDMAFHGEVNGYIIQGVNAINSMPNRNKVLKA
HCCCCEEECCCCCCCCCCCCCCCCHHHHCCEEEECCCCCEEEECCHHHHCCCCHHHHHHH
LSNLKYMVVLDALATETATFWQNADGFNEVNPAEIMTEVFRLPTTCFAEEEGSIVNSGRW
HHCCEEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCEECCCCE
MQWHWKGANPPGEAKPDAEILSGVLMAMRELYKQEGGKLPEPVQAISWDYHNPYSPHAEE
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHH
VARELNGKDLVTQRQLSSFSELKADGSTASACWIYAGSWTEEGNLMARRDNHDPSGKGVT
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCC
PGWAFAWPANRRVLYNRASCDINGKPWDPKRTIVEWVDGKWHGIDVGDFNMKLTPQESAG
CCEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCEEECCCCCCC
PFIMQPEGVGRFFALKLLAEGPFPEHYEPMESPIGLNPLHKVTSNPAVRMLPGVKETLGS
CEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEECCCHHHHHCC
HKDFPYVATTYCVTEHFNFWSTHARLAAISMPETFVEIDEMLAAEKGIANGDWVTVSSKR
CCCCCEEEEEEEHHHCCCHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
GSIETKALVTKRLQPLKVNGQLVHTVGLPRHGSHNALTRKSYSCNVLTTEVGDANTQVPE
CCCHHHHHHHHCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
FKAFLVNITKAEGI
CEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]