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The map label for this gene is yvkC

Identifier: 16080572

GI number: 16080572

Start: 3615793

End: 3618288

Strand: Direct

Name: yvkC

Synonym: BSU35190

Alternate gene names: 16080572

Gene position: 3615793-3618288 (Clockwise)

Preceding gene: 16080567

Following gene: 16080573

Centisome position: 85.77

GC content: 44.07

Gene sequence:

>2496_bases
ATGTATTCTGTTTTATTTCGCCAGGCAGAAGAGTCCAGCCAGCTGGCTGGAGCAAAAGGAATGAATTTGATTAAATTGAC
CAAACACGGTCTTCCTGTTCCGGACGGGTTTATTATTCAAACGAATGCGCTCGCACGTTTTATGGAGGACAACCAGCTTC
AAGAGACTAGTGAAAACGTCGAAGGCGGGATCATTTCCGGAACATTTTCGGATGAGCTGAAAGATGAGCTGACTAGTTCC
TTTTATAAGCTTAGAGAATCATATCGATCCGTAGCCGTGCGTTCTTCGTCTGCTTCGGAAGATTTAGAAGGCGCCTCATT
CGCGGGTCAATATGAAACCTACTTAAATATCAAAACAGAGGAAGAGTTTCTGGCTAAAGTGAAAGAATGCTGGGCCTCAT
TTTTTTCTGGGCGGGTCAGCAGCTATAAGAAAAAAATGAACAATCAAATCGCAGAGCCGTTAATGGGAATAGTCGTTCAG
GGGCTGATCGATTCAGAAATGTCAGGTGTTATCTTCAGCCGCAACCCTGTTACCCATGATGATAGAGAGCTTTTAATCAG
CGCCAGCTACGGGTTGGGTGAAGCTGTTGTTTCAGGAAATGTTACCCCAGACACGTTCATTGTTAATAAATCTTCGTTTG
AGATTCAGAAAGAAATAGGTGCAAAGGAAATCTACATGGAGTCTGCGGCAGAAGGAATTGCTGAAAAAGAAACGAGTGAA
GACATGCGCAGCCGTTTTTGCCTTACAGATGAACAAGTGATTGAATTGGCTGAAATCACAAAAAAAACCGAAGACCTGTA
CGGATATCCTGTTGATATAGAATTTGGAATTGCTGATCATCAAATATACCTTCTGCAAGCTCGCCCGATTACAACCATTG
ATCAGGACAAAAAAGCGGCAGAAGAAAAACGCAGCTTCATGATTACCGACACTGATATGAATGATTTCTGGCTTAACATG
GAGTCTAATATTGAAGGTCCGGTGAGTCCGTTATTTTCATCCTTCATCGTGCCGGCATTGGAATATGGCTTGAAGAAGAG
CATGCAAAAGTTTCCGATTGGTGTAGTTGTTGATGAAGTAAAACTTTATCGCGGACATATTTATTCCAAAAACCAAGGCG
GACAGCAGCCTCCTTCTGAAGACTGCGGCAAAGAGCTTTTCCCGATTTTATCGGAGCATATGTATGACATCATCAATCAC
ACATACCTCCCTTTTTACCGGACACTGGACCAGCTCGCACAAACTGAGCATACCGCAGAAAGCGCACTGGAAGCTTTTCA
AAAACTAAAGGCCTTTTATCTCACGGCTTATGAAGAGCACTTTAATATCGTTTTCCCGCAAATCCTTTTAACAAACAAAC
TGCAAGCGATGTATCAGGACATTCAAGGAGAGTCCGAAAACGCTCATTTTTACGAGATGCTGACAGGAAAAATGAACAAA
TCACTGGAAACGGACCGTTGCTTATGGCTATTTTCTGTGGAAGTTCAGGAGAACCCGAACCTTCTGGCCATTTTTGAAAA
CAACAAGCCTGAACAGCTCCAGGAGAAATTGGAACAAACAGATGAGGGAAGACACTTCCTGAAGAACGTCCATGAATTCT
TGCAAGAATACGGATGGAGATCTGTTAAAAGTCATGATCTGATTGAACAAATCTGGGTGGAAAATCCGTATTTCGCTCTG
GCCAATATTCAAAATTATGTCCGTAATGGCTATCATTTTGACAATGAATTTCAGAAAACAAAAGAAAAACGAGAGAAATT
ATACAATGAATTCTTGGAAAGCATAGAAGATCCCGGTTTACGCACCGAATTTGACCGCTATTATCAATGGACACTGAACT
CTGCAAATATAAAAGATGATCACCACTTTTATATTGACGCCATGCTGGATGCCAAGGCGAGAATCTTTCTGCTGAAGATA
GGTGAATTGCTGGCGGAAAACGGTGTCATTCAAGATCGGGAGGACCTTTGGTTCTTATATGACGACGAAGTGGAACAAGC
GCTTCTTCACCCTGTATCCCTGCAAGAAAAGGCTGAAAAACGCAGACAGATTTTTCATGAGTATGAGCTGGCCCAAGCCC
CGGCCTACCTCGGCACCCCGACAAAAGAACAGCTCAAAGCAGCTGAAGAAATTGTCGGCGCTGTGATAGAGGATGAAAAA
AACACAGAGAATCATATTTTTGGCATTGCGGCATCAAGCGGCATTGCGACAGGTCCGGTGAAAATCATTCGGGACGCCAA
TGAATTTTCTCAATTCGCGCCTGGAGACGTACTCGTTTGCAAGATGACCACACCGCTATGGACCAGCCTGTTTCAAGACG
CCAAAGCGATTATTACAGACACAGGCGGCATTTTGTCTCACGCTGCGATTATTGCCCGTGAATACGGCATTCCAGCCGTT
CTCGGCACACGCACGGCAACCGAAAGACTGCGAGACGGTGACATCATCACTGTTGACGGAAGCAGCGGCAAAATCACAGT
TGTCAGCCGGTCCTGA

Upstream 100 bases:

>100_bases
ATTAAATGATAGTGTTTACTATCATTTTTTAAACACAGGCTCCGCAATAAAAAGACTAGAGAGAAAAACAAAATGAATGA
AGAAAAGAGGGGTTTCAAAT

Downstream 100 bases:

>100_bases
TGCGTCCCCCTCTTTCTTATACAAGCGGGGCGGGTGTATACTTGTCCTGCCAGTATATTCGTGAGGTGAAACGTATTGAG
ACCGACAAACAAGAGAATCC

Product: phosphotransferase

Products: AMP; H+; Phosphoenolpyruvate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 831; Mature: 831

Protein sequence:

>831_residues
MYSVLFRQAEESSQLAGAKGMNLIKLTKHGLPVPDGFIIQTNALARFMEDNQLQETSENVEGGIISGTFSDELKDELTSS
FYKLRESYRSVAVRSSSASEDLEGASFAGQYETYLNIKTEEEFLAKVKECWASFFSGRVSSYKKKMNNQIAEPLMGIVVQ
GLIDSEMSGVIFSRNPVTHDDRELLISASYGLGEAVVSGNVTPDTFIVNKSSFEIQKEIGAKEIYMESAAEGIAEKETSE
DMRSRFCLTDEQVIELAEITKKTEDLYGYPVDIEFGIADHQIYLLQARPITTIDQDKKAAEEKRSFMITDTDMNDFWLNM
ESNIEGPVSPLFSSFIVPALEYGLKKSMQKFPIGVVVDEVKLYRGHIYSKNQGGQQPPSEDCGKELFPILSEHMYDIINH
TYLPFYRTLDQLAQTEHTAESALEAFQKLKAFYLTAYEEHFNIVFPQILLTNKLQAMYQDIQGESENAHFYEMLTGKMNK
SLETDRCLWLFSVEVQENPNLLAIFENNKPEQLQEKLEQTDEGRHFLKNVHEFLQEYGWRSVKSHDLIEQIWVENPYFAL
ANIQNYVRNGYHFDNEFQKTKEKREKLYNEFLESIEDPGLRTEFDRYYQWTLNSANIKDDHHFYIDAMLDAKARIFLLKI
GELLAENGVIQDREDLWFLYDDEVEQALLHPVSLQEKAEKRRQIFHEYELAQAPAYLGTPTKEQLKAAEEIVGAVIEDEK
NTENHIFGIAASSGIATGPVKIIRDANEFSQFAPGDVLVCKMTTPLWTSLFQDAKAIITDTGGILSHAAIIAREYGIPAV
LGTRTATERLRDGDIITVDGSSGKITVVSRS

Sequences:

>Translated_831_residues
MYSVLFRQAEESSQLAGAKGMNLIKLTKHGLPVPDGFIIQTNALARFMEDNQLQETSENVEGGIISGTFSDELKDELTSS
FYKLRESYRSVAVRSSSASEDLEGASFAGQYETYLNIKTEEEFLAKVKECWASFFSGRVSSYKKKMNNQIAEPLMGIVVQ
GLIDSEMSGVIFSRNPVTHDDRELLISASYGLGEAVVSGNVTPDTFIVNKSSFEIQKEIGAKEIYMESAAEGIAEKETSE
DMRSRFCLTDEQVIELAEITKKTEDLYGYPVDIEFGIADHQIYLLQARPITTIDQDKKAAEEKRSFMITDTDMNDFWLNM
ESNIEGPVSPLFSSFIVPALEYGLKKSMQKFPIGVVVDEVKLYRGHIYSKNQGGQQPPSEDCGKELFPILSEHMYDIINH
TYLPFYRTLDQLAQTEHTAESALEAFQKLKAFYLTAYEEHFNIVFPQILLTNKLQAMYQDIQGESENAHFYEMLTGKMNK
SLETDRCLWLFSVEVQENPNLLAIFENNKPEQLQEKLEQTDEGRHFLKNVHEFLQEYGWRSVKSHDLIEQIWVENPYFAL
ANIQNYVRNGYHFDNEFQKTKEKREKLYNEFLESIEDPGLRTEFDRYYQWTLNSANIKDDHHFYIDAMLDAKARIFLLKI
GELLAENGVIQDREDLWFLYDDEVEQALLHPVSLQEKAEKRRQIFHEYELAQAPAYLGTPTKEQLKAAEEIVGAVIEDEK
NTENHIFGIAASSGIATGPVKIIRDANEFSQFAPGDVLVCKMTTPLWTSLFQDAKAIITDTGGILSHAAIIAREYGIPAV
LGTRTATERLRDGDIITVDGSSGKITVVSRS
>Mature_831_residues
MYSVLFRQAEESSQLAGAKGMNLIKLTKHGLPVPDGFIIQTNALARFMEDNQLQETSENVEGGIISGTFSDELKDELTSS
FYKLRESYRSVAVRSSSASEDLEGASFAGQYETYLNIKTEEEFLAKVKECWASFFSGRVSSYKKKMNNQIAEPLMGIVVQ
GLIDSEMSGVIFSRNPVTHDDRELLISASYGLGEAVVSGNVTPDTFIVNKSSFEIQKEIGAKEIYMESAAEGIAEKETSE
DMRSRFCLTDEQVIELAEITKKTEDLYGYPVDIEFGIADHQIYLLQARPITTIDQDKKAAEEKRSFMITDTDMNDFWLNM
ESNIEGPVSPLFSSFIVPALEYGLKKSMQKFPIGVVVDEVKLYRGHIYSKNQGGQQPPSEDCGKELFPILSEHMYDIINH
TYLPFYRTLDQLAQTEHTAESALEAFQKLKAFYLTAYEEHFNIVFPQILLTNKLQAMYQDIQGESENAHFYEMLTGKMNK
SLETDRCLWLFSVEVQENPNLLAIFENNKPEQLQEKLEQTDEGRHFLKNVHEFLQEYGWRSVKSHDLIEQIWVENPYFAL
ANIQNYVRNGYHFDNEFQKTKEKREKLYNEFLESIEDPGLRTEFDRYYQWTLNSANIKDDHHFYIDAMLDAKARIFLLKI
GELLAENGVIQDREDLWFLYDDEVEQALLHPVSLQEKAEKRRQIFHEYELAQAPAYLGTPTKEQLKAAEEIVGAVIEDEK
NTENHIFGIAASSGIATGPVKIIRDANEFSQFAPGDVLVCKMTTPLWTSLFQDAKAIITDTGGILSHAAIIAREYGIPAV
LGTRTATERLRDGDIITVDGSSGKITVVSRS

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1787994, Length=309, Percent_Identity=30.42071197411, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17564524, Length=309, Percent_Identity=30.0970873786408, Blast_Score=126, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YVKC_BACSU (O34796)

Other databases:

- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   A70043
- RefSeq:   NP_391399.1
- ProteinModelPortal:   O34796
- SMR:   O34796
- EnsemblBacteria:   EBBACT00000001542
- GeneID:   936657
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35190
- NMPDR:   fig|224308.1.peg.3525
- GenoList:   BSU35190
- GeneTree:   EBGT00050000001068
- HOGENOM:   HBG338352
- OMA:   EIMIDAS
- ProtClustDB:   CLSK887945
- BioCyc:   BSUB:BSU35190-MONOMER
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.50.30.10

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N; SSF52009 PEP_mobile

EC number: 2.7.9.2 [C]

Molecular weight: Translated: 94449; Mature: 94449

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 787-787

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSVLFRQAEESSQLAGAKGMNLIKLTKHGLPVPDGFIIQTNALARFMEDNQLQETSENV
CCHHHHHHHHHHHHHCCCCCCCEEEEHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCC
EGGIISGTFSDELKDELTSSFYKLRESYRSVAVRSSSASEDLEGASFAGQYETYLNIKTE
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCH
EEFLAKVKECWASFFSGRVSSYKKKMNNQIAEPLMGIVVQGLIDSEMSGVIFSRNPVTHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
DRELLISASYGLGEAVVSGNVTPDTFIVNKSSFEIQKEIGAKEIYMESAAEGIAEKETSE
CCEEEEEECCCCCHHHHCCCCCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
DMRSRFCLTDEQVIELAEITKKTEDLYGYPVDIEFGIADHQIYLLQARPITTIDQDKKAA
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHH
EEKRSFMITDTDMNDFWLNMESNIEGPVSPLFSSFIVPALEYGLKKSMQKFPIGVVVDEV
HHHCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
KLYRGHIYSKNQGGQQPPSEDCGKELFPILSEHMYDIINHTYLPFYRTLDQLAQTEHTAE
HHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SALEAFQKLKAFYLTAYEEHFNIVFPQILLTNKLQAMYQDIQGESENAHFYEMLTGKMNK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
SLETDRCLWLFSVEVQENPNLLAIFENNKPEQLQEKLEQTDEGRHFLKNVHEFLQEYGWR
CCCCCCEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
SVKSHDLIEQIWVENPYFALANIQNYVRNGYHFDNEFQKTKEKREKLYNEFLESIEDPGL
CCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
RTEFDRYYQWTLNSANIKDDHHFYIDAMLDAKARIFLLKIGELLAENGVIQDREDLWFLY
CCHHHHHEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEE
DDEVEQALLHPVSLQEKAEKRRQIFHEYELAQAPAYLGTPTKEQLKAAEEIVGAVIEDEK
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
NTENHIFGIAASSGIATGPVKIIRDANEFSQFAPGDVLVCKMTTPLWTSLFQDAKAIITD
CCCCEEEEEEECCCCCCCCHHHHHCCHHHHHCCCCCEEEEEECCHHHHHHHHCCHHHHCC
TGGILSHAAIIAREYGIPAVLGTRTATERLRDGDIITVDGSSGKITVVSRS
CCHHHHHHHHHHHHCCCCEEECCCHHHHHHCCCCEEEEECCCCEEEEEECC
>Mature Secondary Structure
MYSVLFRQAEESSQLAGAKGMNLIKLTKHGLPVPDGFIIQTNALARFMEDNQLQETSENV
CCHHHHHHHHHHHHHCCCCCCCEEEEHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCC
EGGIISGTFSDELKDELTSSFYKLRESYRSVAVRSSSASEDLEGASFAGQYETYLNIKTE
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCH
EEFLAKVKECWASFFSGRVSSYKKKMNNQIAEPLMGIVVQGLIDSEMSGVIFSRNPVTHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
DRELLISASYGLGEAVVSGNVTPDTFIVNKSSFEIQKEIGAKEIYMESAAEGIAEKETSE
CCEEEEEECCCCCHHHHCCCCCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
DMRSRFCLTDEQVIELAEITKKTEDLYGYPVDIEFGIADHQIYLLQARPITTIDQDKKAA
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHH
EEKRSFMITDTDMNDFWLNMESNIEGPVSPLFSSFIVPALEYGLKKSMQKFPIGVVVDEV
HHHCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
KLYRGHIYSKNQGGQQPPSEDCGKELFPILSEHMYDIINHTYLPFYRTLDQLAQTEHTAE
HHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SALEAFQKLKAFYLTAYEEHFNIVFPQILLTNKLQAMYQDIQGESENAHFYEMLTGKMNK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
SLETDRCLWLFSVEVQENPNLLAIFENNKPEQLQEKLEQTDEGRHFLKNVHEFLQEYGWR
CCCCCCEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
SVKSHDLIEQIWVENPYFALANIQNYVRNGYHFDNEFQKTKEKREKLYNEFLESIEDPGL
CCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
RTEFDRYYQWTLNSANIKDDHHFYIDAMLDAKARIFLLKIGELLAENGVIQDREDLWFLY
CCHHHHHEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEE
DDEVEQALLHPVSLQEKAEKRRQIFHEYELAQAPAYLGTPTKEQLKAAEEIVGAVIEDEK
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
NTENHIFGIAASSGIATGPVKIIRDANEFSQFAPGDVLVCKMTTPLWTSLFQDAKAIITD
CCCCEEEEEEECCCCCCCCHHHHHCCHHHHHCCCCCEEEEEECCHHHHHHHHCCHHHHCC
TGGILSHAAIIAREYGIPAVLGTRTATERLRDGDIITVDGSSGKITVVSRS
CCHHHHHHHHHHHHCCCCEEECCCHHHHHHCCCCEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.083 {pyruvate}} 10.5 {phosphate}} 0.028 {ATP}} [C]

Substrates: ATP; H2O; Pyruvate [C]

Specific reaction: ATP + H2O + Pyruvate --> AMP + (2) H+ + Phosphoenolpyruvate + Phosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377