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The map label for this gene is yvcT

Identifier: 16080521

GI number: 16080521

Start: 3562566

End: 3563543

Strand: Direct

Name: yvcT

Synonym: BSU34680

Alternate gene names: 16080521

Gene position: 3562566-3563543 (Clockwise)

Preceding gene: 16080518

Following gene: 255767771

Centisome position: 84.51

GC content: 45.09

Gene sequence:

>978_bases
TTGTTGAAACCATTTGTATTTATCACAAAACCGATTCCTGAAGAGATTGAAGCATTCATCGGTGAACATTGCCGTTATGA
AGTATGGCAGGAGGATACGCTTCCGAGTGACGTGCTATTTGAAAAATTAAAAGAAGCGGAGGGGCTTTTAACATCAGGGA
CGTCCGGACCGTCAATCAATCGTGAGTTGCTGGAACACGCGCCTAAGCTCAAAGTGGTCAGCAACCAATCAGTAGGCTAC
GATAACTTCGATATAGAAGCAATGAAAGAAAGAGGAGTCGTCGGGACTCATACACCTTATACACTGGATGACACAGTTGC
AGATCTGGCTTTTTCACTGATTCTGTCTTCCGCCAGACGTGTTGCCGAACTGGACCGTTTTGTCAGAGCAGGAAAATGGG
GAACCGTGGAAGAGGAAGCGCTTTTCGGAATTGATGTTCATCATCAAACGTTAGGCATTATCGGCATGGGCAGAATTGGA
GAACAAGCAGCGAGACGCGCCAAATTTGGTTTTGATATGGAAGTGCTTTATCATAACCGACATCGCAAACAAGAAACAGA
AGACAGCATCGGCGTTAAGTATGCAGAGCTTGATACATTGCTGGAGCAATCTGATTTTATTCTTCTCATTACGCCGCTGA
CTGATGAAACCTATCACATGATAGGAGAACGCGAATTTAAGTTGATGAAGAATTCAGCAATTTTCGTCAATATTTCCCGG
GGAAAGACAGTTGATGAAAAAGCACTGATTCGCGCTCTTCAGGAAGGCTGGATCAGGGGAGCCGGCTTAGATGTATACGA
AAAAGAGCCTGTCACCCAAGATAATCCGCTCCTGCAGCTGGATAATGTCACGCTGCTTCCTCATATCGGTTCAGCAACAG
CTAAAGTGCGCTTCAACATGTGCAAACAGGCCGCCGAAAATATGCTCGCAGCGATCCAAGGGCAAACACCGAAAAATCTT
ACAAGAGAATTTCAATAA

Upstream 100 bases:

>100_bases
TAAGTTCAAAAGAACCAGAAATTAAAAAAACGCAGCTATATTTTTAACATCTATTTATTTTCGATATAATGAATTAGACA
ACAGCCATAAAGGGGGAGCC

Downstream 100 bases:

>100_bases
ATCAAAAATCCGGTCTGATAACAGACCGGATTTCATATTACATACGCTGAAGAACAGCACGCTTATAAATATGACGGGTG
ACGATATAATAAAGAGCCTG

Product: 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2KR

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MLKPFVFITKPIPEEIEAFIGEHCRYEVWQEDTLPSDVLFEKLKEAEGLLTSGTSGPSINRELLEHAPKLKVVSNQSVGY
DNFDIEAMKERGVVGTHTPYTLDDTVADLAFSLILSSARRVAELDRFVRAGKWGTVEEEALFGIDVHHQTLGIIGMGRIG
EQAARRAKFGFDMEVLYHNRHRKQETEDSIGVKYAELDTLLEQSDFILLITPLTDETYHMIGEREFKLMKNSAIFVNISR
GKTVDEKALIRALQEGWIRGAGLDVYEKEPVTQDNPLLQLDNVTLLPHIGSATAKVRFNMCKQAAENMLAAIQGQTPKNL
TREFQ

Sequences:

>Translated_325_residues
MLKPFVFITKPIPEEIEAFIGEHCRYEVWQEDTLPSDVLFEKLKEAEGLLTSGTSGPSINRELLEHAPKLKVVSNQSVGY
DNFDIEAMKERGVVGTHTPYTLDDTVADLAFSLILSSARRVAELDRFVRAGKWGTVEEEALFGIDVHHQTLGIIGMGRIG
EQAARRAKFGFDMEVLYHNRHRKQETEDSIGVKYAELDTLLEQSDFILLITPLTDETYHMIGEREFKLMKNSAIFVNISR
GKTVDEKALIRALQEGWIRGAGLDVYEKEPVTQDNPLLQLDNVTLLPHIGSATAKVRFNMCKQAAENMLAAIQGQTPKNL
TREFQ
>Mature_325_residues
MLKPFVFITKPIPEEIEAFIGEHCRYEVWQEDTLPSDVLFEKLKEAEGLLTSGTSGPSINRELLEHAPKLKVVSNQSVGY
DNFDIEAMKERGVVGTHTPYTLDDTVADLAFSLILSSARRVAELDRFVRAGKWGTVEEEALFGIDVHHQTLGIIGMGRIG
EQAARRAKFGFDMEVLYHNRHRKQETEDSIGVKYAELDTLLEQSDFILLITPLTDETYHMIGEREFKLMKNSAIFVNISR
GKTVDEKALIRALQEGWIRGAGLDVYEKEPVTQDNPLLQLDNVTLLPHIGSATAKVRFNMCKQAAENMLAAIQGQTPKNL
TREFQ

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Homologues:

Organism=Homo sapiens, GI6912396, Length=315, Percent_Identity=38.0952380952381, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI23308577, Length=290, Percent_Identity=31.0344827586207, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI61743967, Length=285, Percent_Identity=35.0877192982456, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI145580578, Length=285, Percent_Identity=36.4912280701754, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI4557499, Length=285, Percent_Identity=36.4912280701754, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI4557497, Length=285, Percent_Identity=35.0877192982456, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI145580575, Length=285, Percent_Identity=36.4912280701754, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI87082289, Length=321, Percent_Identity=47.3520249221184, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1787645, Length=275, Percent_Identity=31.2727272727273, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789279, Length=255, Percent_Identity=30.1960784313725, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081824, Length=253, Percent_Identity=25.6916996047431, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=260, Percent_Identity=30.3846153846154, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=273, Percent_Identity=29.6703296703297, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=238, Percent_Identity=34.453781512605, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320925, Length=283, Percent_Identity=31.4487632508834, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6322116, Length=283, Percent_Identity=31.4487632508834, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6321253, Length=298, Percent_Identity=26.1744966442953, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324964, Length=279, Percent_Identity=25.4480286738351, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325144, Length=146, Percent_Identity=32.8767123287671, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45551003, Length=316, Percent_Identity=38.2911392405063, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28574284, Length=316, Percent_Identity=38.2911392405063, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24585514, Length=316, Percent_Identity=38.2911392405063, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28574282, Length=316, Percent_Identity=38.2911392405063, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI45552429, Length=316, Percent_Identity=38.2911392405063, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28574286, Length=320, Percent_Identity=35.9375, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28571528, Length=312, Percent_Identity=33.974358974359, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24585516, Length=258, Percent_Identity=34.1085271317829, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24646446, Length=285, Percent_Identity=34.0350877192982, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24646448, Length=285, Percent_Identity=34.0350877192982, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24646452, Length=285, Percent_Identity=34.0350877192982, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24646450, Length=285, Percent_Identity=34.0350877192982, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI62472511, Length=269, Percent_Identity=33.8289962825279, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI19921140, Length=288, Percent_Identity=27.7777777777778, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TKRA_BACSU (O32264)

Other databases:

- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   H70032
- RefSeq:   NP_391348.1
- ProteinModelPortal:   O32264
- SMR:   O32264
- EnsemblBacteria:   EBBACT00000003849
- GeneID:   936519
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34680
- NMPDR:   fig|224308.1.peg.3474
- GenoList:   BSU34680
- GeneTree:   EBGT00050000001763
- HOGENOM:   HBG731446
- OMA:   FMKETAI
- PhylomeDB:   O32264
- ProtClustDB:   CLSK887930
- BioCyc:   BSUB:BSU34680-MONOMER
- BRENDA:   1.1.1.215
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.215

Molecular weight: Translated: 36619; Mature: 36619

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 240-240 ACT_SITE 269-269 ACT_SITE 288-288 BINDING 211-211 BINDING 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPFVFITKPIPEEIEAFIGEHCRYEVWQEDTLPSDVLFEKLKEAEGLLTSGTSGPSIN
CCCCEEEEECCCCHHHHHHHCCHHEEECCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCC
RELLEHAPKLKVVSNQSVGYDNFDIEAMKERGVVGTHTPYTLDDTVADLAFSLILSSARR
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAELDRFVRAGKWGTVEEEALFGIDVHHQTLGIIGMGRIGEQAARRAKFGFDMEVLYHNR
HHHHHHHHHCCCCCCCCHHHEEEEEECHHHCCEEECCHHHHHHHHHHHCCCCHHHHHHCH
HRKQETEDSIGVKYAELDTLLEQSDFILLITPLTDETYHMIGEREFKLMKNSAIFVNISR
HHHHCCHHHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHCCHHHHEECCCEEEEEECC
GKTVDEKALIRALQEGWIRGAGLDVYEKEPVTQDNPLLQLDNVTLLPHIGSATAKVRFNM
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCHHHHHHHH
CKQAAENMLAAIQGQTPKNLTREFQ
HHHHHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure
MLKPFVFITKPIPEEIEAFIGEHCRYEVWQEDTLPSDVLFEKLKEAEGLLTSGTSGPSIN
CCCCEEEEECCCCHHHHHHHCCHHEEECCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCC
RELLEHAPKLKVVSNQSVGYDNFDIEAMKERGVVGTHTPYTLDDTVADLAFSLILSSARR
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAELDRFVRAGKWGTVEEEALFGIDVHHQTLGIIGMGRIGEQAARRAKFGFDMEVLYHNR
HHHHHHHHHCCCCCCCCHHHEEEEEECHHHCCEEECCHHHHHHHHHHHCCCCHHHHHHCH
HRKQETEDSIGVKYAELDTLLEQSDFILLITPLTDETYHMIGEREFKLMKNSAIFVNISR
HHHHCCHHHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHCCHHHHEECCCEEEEEECC
GKTVDEKALIRALQEGWIRGAGLDVYEKEPVTQDNPLLQLDNVTLLPHIGSATAKVRFNM
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCHHHHHHHH
CKQAAENMLAAIQGQTPKNLTREFQ
HHHHHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377