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The map label for this gene is levB

Identifier: 16080499

GI number: 16080499

Start: 3537507

End: 3539057

Strand: Direct

Name: levB

Synonym: BSU34460

Alternate gene names: 16080499

Gene position: 3537507-3539057 (Clockwise)

Preceding gene: 16080498

Following gene: 16080500

Centisome position: 83.91

GC content: 45.2

Gene sequence:

>1551_bases
ATGAACTATATAAAAGCAGGCAAATGGCTAACCGTATTCCTAACCTTTTTAGGAATATTGCTGTTTATCGACTTATTTCC
AAAAGAAGAACATGACCAAAAAACAAAATCAAAACAGAAGCCGGACTACCGGGCGGCATATCATTTTACGACGCCGGATA
AATGGAAAAATGACCCTCAAAAACCGATCTATTTTGATGGCAAGTATCATTATTTCTATCTATATAACCGGGATTACCCA
AAAGGCAACGGCACAGAATGGCGCCATGCCGTCTCAGAGGATTTGGTGCACTGGACCGATGAAGGCGTGGCGATTCCGAA
ATATACAAACCCGGACGGTGATATTTGGACCGGTTCCGTCGTGGTGGATAAAGAGAACACAGCCGGCTTCGGGAAAAATG
CGCTTGTCGCGATTGTGACACAGCCCTCAGCCAAAGACAAAAAACAGGAACAATATTTGTGGTACAGCACAGATAAGGGA
AAATCATTCAAATTCTACAGTGGCAACCCCGTTATGCCTAATCCGGGCACAGACGATTTCAGAGATCCGAAAGTCATATG
GGATGACCAAGATAACAAATGGGTCATGGTCATGGCTGAAGGATCAAAAATCGGCTTTTATGAATCCGATAATCTTAAGG
ACTGGCATTACACAAGCGGATTCTTCCCAGAACAGGCGGGAATGGTGGAATGTCCCGACCTCTACATGATGCGGGCAAGC
GACGGAACGAATAAGTGGGTTCTCGGTGCCAGCGCGAATGGCAAACCGTGGGGCAAACCAAATACGTACGCCTACTGGAC
CGGAAGCTTCGACGGAAAAGAATTCAAAGCGGATCAGACTGAAGCCCAATGGCTTGACTATGGCTTCGACTGGTATGGCG
GTGTGACGTTCGAAGACAGCAAAAGCACAGATCCATTAGAAAAGCGGTATGCGCTTGCCTGGATGAACAATTGGGATTAT
GCCAACAACACCCCGACAATGAAGAACGGCTTTAACGGCACAGATTCTGTCATACGCGAACTCCGGCTGAAGGAGCAGGA
TGGAACATACAGCCTCGTCTCACAGCCGATTGAAGCTTTGGAGCAGCTGACTGTTTCAACTGACGAAATAGAGGATCAGG
ATGTGAACGGATCAAAAACATTGTCGATAACAGGTGATACGTACCAGCTTGATACGGATCTTTCTTGGTCAGAGCTAAAG
AATGCAGGCGTCAGGCTCAGAGAATCAGAAGACCAAAAACGCCATATTGATGTCGGCATTTTCGCTGAAGGCGGCTATGC
TTATGTCAATAGAGCAGCCACAAATCAGCCTGACAAAAGCAATACCTATGTCGAAAGCAAAGCTCCTTATGATGTAAACA
AACGTAAGGTGCATTTGAAAATACTTGTCGATAAAACAACGATAGAAGTATTTGTTGGCGACGGGAAAACCGTTTTTTCA
AATGAAGTCTTCCCAAAGCCTGAAGATAAAGGAATTACCCTTTATTCTGACGGCGGCACAGCCTCCTTCAAAAATATAAC
AGTGAAACACTTTGATTCGATTCATGAATAA

Upstream 100 bases:

>100_bases
CAAGGACAATTAACAGTTAACAAATAAAAACGCAAAAGAAAATGCCGATATCCTATTGGCATTTTCTTTTATTTCTTATC
AACATAAAGGTGAATCCCAT

Downstream 100 bases:

>100_bases
AAACAGGGGCGGCGCAGGCTGCCCCTGTTTTTTTATTAGGAGCGGTATTCTCTGTTACATATTGGGCATTGTAAGGAATA
TAAGGCTGGTTTCAAAGGAG

Product: endolevanase

Products: NA

Alternate protein names: 2,6-beta-fructan 6-levanbiohydrolase; Endo-levanase

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MNYIKAGKWLTVFLTFLGILLFIDLFPKEEHDQKTKSKQKPDYRAAYHFTTPDKWKNDPQKPIYFDGKYHYFYLYNRDYP
KGNGTEWRHAVSEDLVHWTDEGVAIPKYTNPDGDIWTGSVVVDKENTAGFGKNALVAIVTQPSAKDKKQEQYLWYSTDKG
KSFKFYSGNPVMPNPGTDDFRDPKVIWDDQDNKWVMVMAEGSKIGFYESDNLKDWHYTSGFFPEQAGMVECPDLYMMRAS
DGTNKWVLGASANGKPWGKPNTYAYWTGSFDGKEFKADQTEAQWLDYGFDWYGGVTFEDSKSTDPLEKRYALAWMNNWDY
ANNTPTMKNGFNGTDSVIRELRLKEQDGTYSLVSQPIEALEQLTVSTDEIEDQDVNGSKTLSITGDTYQLDTDLSWSELK
NAGVRLRESEDQKRHIDVGIFAEGGYAYVNRAATNQPDKSNTYVESKAPYDVNKRKVHLKILVDKTTIEVFVGDGKTVFS
NEVFPKPEDKGITLYSDGGTASFKNITVKHFDSIHE

Sequences:

>Translated_516_residues
MNYIKAGKWLTVFLTFLGILLFIDLFPKEEHDQKTKSKQKPDYRAAYHFTTPDKWKNDPQKPIYFDGKYHYFYLYNRDYP
KGNGTEWRHAVSEDLVHWTDEGVAIPKYTNPDGDIWTGSVVVDKENTAGFGKNALVAIVTQPSAKDKKQEQYLWYSTDKG
KSFKFYSGNPVMPNPGTDDFRDPKVIWDDQDNKWVMVMAEGSKIGFYESDNLKDWHYTSGFFPEQAGMVECPDLYMMRAS
DGTNKWVLGASANGKPWGKPNTYAYWTGSFDGKEFKADQTEAQWLDYGFDWYGGVTFEDSKSTDPLEKRYALAWMNNWDY
ANNTPTMKNGFNGTDSVIRELRLKEQDGTYSLVSQPIEALEQLTVSTDEIEDQDVNGSKTLSITGDTYQLDTDLSWSELK
NAGVRLRESEDQKRHIDVGIFAEGGYAYVNRAATNQPDKSNTYVESKAPYDVNKRKVHLKILVDKTTIEVFVGDGKTVFS
NEVFPKPEDKGITLYSDGGTASFKNITVKHFDSIHE
>Mature_516_residues
MNYIKAGKWLTVFLTFLGILLFIDLFPKEEHDQKTKSKQKPDYRAAYHFTTPDKWKNDPQKPIYFDGKYHYFYLYNRDYP
KGNGTEWRHAVSEDLVHWTDEGVAIPKYTNPDGDIWTGSVVVDKENTAGFGKNALVAIVTQPSAKDKKQEQYLWYSTDKG
KSFKFYSGNPVMPNPGTDDFRDPKVIWDDQDNKWVMVMAEGSKIGFYESDNLKDWHYTSGFFPEQAGMVECPDLYMMRAS
DGTNKWVLGASANGKPWGKPNTYAYWTGSFDGKEFKADQTEAQWLDYGFDWYGGVTFEDSKSTDPLEKRYALAWMNNWDY
ANNTPTMKNGFNGTDSVIRELRLKEQDGTYSLVSQPIEALEQLTVSTDEIEDQDVNGSKTLSITGDTYQLDTDLSWSELK
NAGVRLRESEDQKRHIDVGIFAEGGYAYVNRAATNQPDKSNTYVESKAPYDVNKRKVHLKILVDKTTIEVFVGDGKTVFS
NEVFPKPEDKGITLYSDGGTASFKNITVKHFDSIHE

Specific function: Catalyzes the degradation of levan mainly into levanbiose (difructose). Is not active on sucrose

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=484, Percent_Identity=29.1322314049587, Blast_Score=154, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEVB_BACSU (O07003)

Other databases:

- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   E70035
- RefSeq:   NP_391326.1
- HSSP:   O33833
- ProteinModelPortal:   O07003
- SMR:   O07003
- EnsemblBacteria:   EBBACT00000000473
- GeneID:   938616
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34460
- NMPDR:   fig|224308.1.peg.3452
- GenoList:   BSU34460
- GeneTree:   EBGT00050000001979
- HOGENOM:   HBG693902
- OMA:   TYAYWTG
- PhylomeDB:   O07003
- ProtClustDB:   CLSK882933
- BioCyc:   BSUB:BSU34460-MONOMER
- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR013189
- InterPro:   IPR013148
- SMART:   SM00640

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N; SSF49899 ConA_like_lec_gl

EC number: =3.2.1.64

Molecular weight: Translated: 58979; Mature: 58979

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: ACT_SITE 58-58 BINDING 230-230 BINDING 318-318

Signals:

None

Transmembrane regions:

HASH(0x12065d00)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYIKAGKWLTVFLTFLGILLFIDLFPKEEHDQKTKSKQKPDYRAAYHFTTPDKWKNDPQ
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCHHCCCCCC
KPIYFDGKYHYFYLYNRDYPKGNGTEWRHAVSEDLVHWTDEGVAIPKYTNPDGDIWTGSV
CCEEECCEEEEEEEEECCCCCCCCCHHHHHHHHHHHEECCCCEECCCCCCCCCCEEECEE
VVDKENTAGFGKNALVAIVTQPSAKDKKQEQYLWYSTDKGKSFKFYSGNPVMPNPGTDDF
EEECCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCC
RDPKVIWDDQDNKWVMVMAEGSKIGFYESDNLKDWHYTSGFFPEQAGMVECPDLYMMRAS
CCCEEEEECCCCCEEEEEECCCEEEEEECCCCCCEEECCCCCCCCCCCEECCCEEEEECC
DGTNKWVLGASANGKPWGKPNTYAYWTGSFDGKEFKADQTEAQWLDYGFDWYGGVTFEDS
CCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCEEECCC
KSTDPLEKRYALAWMNNWDYANNTPTMKNGFNGTDSVIRELRLKEQDGTYSLVSQPIEAL
CCCCHHHHEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
EQLTVSTDEIEDQDVNGSKTLSITGDTYQLDTDLSWSELKNAGVRLRESEDQKRHIDVGI
HHHCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCHHHHHHCCEEEECCCCCCEEEEEEE
FAEGGYAYVNRAATNQPDKSNTYVESKAPYDVNKRKVHLKILVDKTTIEVFVGDGKTVFS
EECCCEEEEECCCCCCCCCCCCEEECCCCCCCCCEEEEEEEEEEEEEEEEEECCCCEEEC
NEVFPKPEDKGITLYSDGGTASFKNITVKHFDSIHE
CCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHCCCH
>Mature Secondary Structure
MNYIKAGKWLTVFLTFLGILLFIDLFPKEEHDQKTKSKQKPDYRAAYHFTTPDKWKNDPQ
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCHHCCCCCC
KPIYFDGKYHYFYLYNRDYPKGNGTEWRHAVSEDLVHWTDEGVAIPKYTNPDGDIWTGSV
CCEEECCEEEEEEEEECCCCCCCCCHHHHHHHHHHHEECCCCEECCCCCCCCCCEEECEE
VVDKENTAGFGKNALVAIVTQPSAKDKKQEQYLWYSTDKGKSFKFYSGNPVMPNPGTDDF
EEECCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCC
RDPKVIWDDQDNKWVMVMAEGSKIGFYESDNLKDWHYTSGFFPEQAGMVECPDLYMMRAS
CCCEEEEECCCCCEEEEEECCCEEEEEECCCCCCEEECCCCCCCCCCCEECCCEEEEECC
DGTNKWVLGASANGKPWGKPNTYAYWTGSFDGKEFKADQTEAQWLDYGFDWYGGVTFEDS
CCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCEEECCC
KSTDPLEKRYALAWMNNWDYANNTPTMKNGFNGTDSVIRELRLKEQDGTYSLVSQPIEAL
CCCCHHHHEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
EQLTVSTDEIEDQDVNGSKTLSITGDTYQLDTDLSWSELKNAGVRLRESEDQKRHIDVGI
HHHCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCHHHHHHCCEEEECCCCCCEEEEEEE
FAEGGYAYVNRAATNQPDKSNTYVESKAPYDVNKRKVHLKILVDKTTIEVFVGDGKTVFS
EECCCEEEEECCCCCCCCCCCCEEECCCCCCCCCEEEEEEEEEEEEEEEEEECCCCEEEC
NEVFPKPEDKGITLYSDGGTASFKNITVKHFDSIHE
CCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377