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The map label for this gene is ald

Identifier: 16080244

GI number: 16080244

Start: 3278325

End: 3279461

Strand: Direct

Name: ald

Synonym: BSU31930

Alternate gene names: 16080244

Gene position: 3278325-3279461 (Clockwise)

Preceding gene: 255767729

Following gene: 255767730

Centisome position: 77.77

GC content: 49.69

Gene sequence:

>1137_bases
ATGATCATAGGGGTTCCTAAAGAGATAAAAAACAATGAAAACCGTGTCGCATTAACACCCGGGGGCGTTTCTCAGCTCAT
TTCAAACGGCCACCGGGTGCTGGTTGAAACAGGCGCGGGCCTTGGAAGCGGATTTGAAAATGAAGCCTATGAGTCAGCAG
GAGCGGAAATCATTGCTGATCCGAAGCAGGTCTGGGACGCCGAAATGGTCATGAAAGTAAAAGAACCGCTGCCGGAAGAA
TATGTTTATTTTCGCAAAGGACTTGTGCTGTTTACGTACCTTCATTTAGCAGCTGAGCCTGAGCTTGCACAGGCCTTGAA
GGATAAAGGAGTAACTGCCATCGCATATGAAACGGTCAGTGAAGGCCGGACATTGCCTCTTCTGACGCCAATGTCAGAGG
TTGCGGGCAGAATGGCAGCGCAAATCGGCGCTCAATTCTTAGAAAAGCCTAAAGGCGGAAAAGGCATTCTGCTTGCCGGG
GTGCCTGGCGTTTCCCGCGGAAAAGTAACAATTATCGGAGGAGGCGTTGTCGGGACAAACGCGGCGAAAATGGCTGTCGG
CCTCGGTGCAGATGTGACGATCATTGACTTAAACGCAGACCGCTTGCGCCAGCTTGATGACATCTTCGGCCATCAGATTA
AAACGTTAATTTCTAATCCGGTCAATATTGCTGATGCTGTGGCGGAAGCGGATCTCCTCATTTGCGCGGTATTAATTCCG
GGTGCTAAAGCTCCGACTCTTGTCACTGAGGAAATGGTAAAACAAATGAAACCCGGTTCAGTTATTGTTGATGTAGCGAT
CGACCAAGGCGGCATCGTCGAAACTGTCGACCATATCACAACACATGATCAGCCAACATATGAAAAACACGGGGTTGTGC
ATTATGCTGTAGCGAACATGCCAGGCGCAGTCCCTCGTACATCAACAATCGCCCTGACTAACGTTACTGTTCCATACGCG
CTGCAAATCGCGAACAAAGGGGCAGTAAAAGCGCTCGCAGACAATACGGCACTGAGAGCGGGTTTAAACACCGCAAACGG
ACACGTGACCTATGAAGCTGTAGCAAGAGATCTAGGCTATGAGTATGTTCCTGCCGAGAAAGCTTTACAGGATGAATCAT
CTGTGGCGGGTGCTTAA

Upstream 100 bases:

>100_bases
ATTTCACAAATCCATGTTTTTTTATTTTCTTAATCAAACAAAGAATTTTCCAAAATATCAAGCTACACTAAAAATATCAC
ATATACAGGAGGAGACAGAT

Downstream 100 bases:

>100_bases
TTCACAATAAGCTTGCAGAAAGATTTCTGCAGGCTTTTTTATTTTTTAAAAGGAAAAAAGAGACCATTTCACGAATTATG
ACTATGAAGTTGATCATAAA

Product: L-alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVETGAGLGSGFENEAYESAGAEIIADPKQVWDAEMVMKVKEPLPEE
YVYFRKGLVLFTYLHLAAEPELAQALKDKGVTAIAYETVSEGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAG
VPGVSRGKVTIIGGGVVGTNAAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNPVNIADAVAEADLLICAVLIP
GAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDQPTYEKHGVVHYAVANMPGAVPRTSTIALTNVTVPYA
LQIANKGAVKALADNTALRAGLNTANGHVTYEAVARDLGYEYVPAEKALQDESSVAGA

Sequences:

>Translated_378_residues
MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVETGAGLGSGFENEAYESAGAEIIADPKQVWDAEMVMKVKEPLPEE
YVYFRKGLVLFTYLHLAAEPELAQALKDKGVTAIAYETVSEGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAG
VPGVSRGKVTIIGGGVVGTNAAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNPVNIADAVAEADLLICAVLIP
GAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDQPTYEKHGVVHYAVANMPGAVPRTSTIALTNVTVPYA
LQIANKGAVKALADNTALRAGLNTANGHVTYEAVARDLGYEYVPAEKALQDESSVAGA
>Mature_378_residues
MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVETGAGLGSGFENEAYESAGAEIIADPKQVWDAEMVMKVKEPLPEE
YVYFRKGLVLFTYLHLAAEPELAQALKDKGVTAIAYETVSEGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAG
VPGVSRGKVTIIGGGVVGTNAAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNPVNIADAVAEADLLICAVLIP
GAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDQPTYEKHGVVHYAVANMPGAVPRTSTIALTNVTVPYA
LQIANKGAVKALADNTALRAGLNTANGHVTYEAVARDLGYEYVPAEKALQDESSVAGA

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family

Homologues:

Organism=Homo sapiens, GI122939155, Length=348, Percent_Identity=31.6091954022989, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI122939153, Length=348, Percent_Identity=31.6091954022989, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI1787887, Length=378, Percent_Identity=31.4814814814815, Blast_Score=147, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17550456, Length=373, Percent_Identity=30.0268096514745, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHA_BACSU (Q08352)

Other databases:

- EMBL:   L20916
- EMBL:   Z82015
- EMBL:   AL009126
- PIR:   A49337
- RefSeq:   NP_391071.1
- ProteinModelPortal:   Q08352
- SMR:   Q08352
- EnsemblBacteria:   EBBACT00000003424
- GeneID:   936557
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31930
- NMPDR:   fig|224308.1.peg.3196
- GenoList:   BSU31930
- GeneTree:   EBGT00050000000823
- HOGENOM:   HBG289735
- OMA:   EADLLIC
- PhylomeDB:   Q08352
- ProtClustDB:   CLSK887816
- BioCyc:   BSUB:BSU31930-MONOMER
- BioCyc:   MetaCyc:MONOMER-12812
- BRENDA:   1.4.1.1
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141
- PIRSF:   PIRSF000183
- TIGRFAMs:   TIGR00518

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N

EC number: =1.4.1.1

Molecular weight: Translated: 39684; Mature: 39684

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00836 ALADH_PNT_1; PS00837 ALADH_PNT_2

Important sites: ACT_SITE 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVETGAGLGSGFENEAYESAGAEIIAD
CEECCCHHHCCCCCEEEECCCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHCCCEEEEC
PKQVWDAEMVMKVKEPLPEEYVYFRKGLVLFTYLHLAAEPELAQALKDKGVTAIAYETVS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEHHH
EGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAGVPGVSRGKVTIIGGGVVGTN
CCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCEECCC
AAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNPVNIADAVAEADLLICAVLIP
HHHHEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECC
GAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDQPTYEKHGVVHYAVANM
CCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHCCEEEEEECCC
PGAVPRTSTIALTNVTVPYALQIANKGAVKALADNTALRAGLNTANGHVTYEAVARDLGY
CCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHCCHHHHCCCCCCCCCEEHHHHHHHCCC
EYVPAEKALQDESSVAGA
CCCCHHHHHCCCHHCCCC
>Mature Secondary Structure
MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVETGAGLGSGFENEAYESAGAEIIAD
CEECCCHHHCCCCCEEEECCCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHCCCEEEEC
PKQVWDAEMVMKVKEPLPEEYVYFRKGLVLFTYLHLAAEPELAQALKDKGVTAIAYETVS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEHHH
EGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAGVPGVSRGKVTIIGGGVVGTN
CCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCEECCC
AAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNPVNIADAVAEADLLICAVLIP
HHHHEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECC
GAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDQPTYEKHGVVHYAVANM
CCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHCCEEEEEECCC
PGAVPRTSTIALTNVTVPYALQIANKGAVKALADNTALRAGLNTANGHVTYEAVARDLGY
CCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHCCHHHHCCCCCCCCCEEHHHHHHHCCC
EYVPAEKALQDESSVAGA
CCCCHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377