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The map label for this gene is yufM

Identifier: 16080205

GI number: 16080205

Start: 3238751

End: 3239458

Strand: Direct

Name: yufM

Synonym: BSU31530

Alternate gene names: 16080205

Gene position: 3238751-3239458 (Clockwise)

Preceding gene: 16080204

Following gene: 255767718

Centisome position: 76.83

GC content: 40.82

Gene sequence:

>708_bases
ATGATTAATGTACTAATAGTTGAAGATGACCCCATGGTGGGTGAATTAAATAAACGATACTTAAGCCAAATAGATGGCTT
TCAGCTGAAAGGCATCGCTTCTTCCTTTCAAAGCGCTCTGCATATTTTAGGGGAGCACCATATTGACCTCATTTTGCTTG
ATATTTATATGCCGGGAAAAAACGGGCTGGAACTGCTGACGGAATTGAGAGCCCAAAATGAAGCGGTTGACGTGATTGTC
ATCTCAGCGGCCAGCGAGCTTGACGTGATCAAGAAAACACTTCGGTACGGAGCTGTTGATTATTTAATCAAACCGTTTGA
ATTTGAACGATTCCAAACGGCTCTGTCTGATTACAGACGAAAACAAAAGGTGTATTCAACCCATCGCAACATGAGCCAAA
AAGAACTGGACGCCGAGCTTTTTCAAAAGAAAGAAGCAACAGAAAAAGTGCAGCTTCCTAAAGGCCTGACAAAAAGCACG
CTGAAGCTGATCTGGTCCAGTATCCAGTCATTTGAAAATGAATCTTTTACGACAGAAGACTTGGCAAAGCACACAGAAAT
CTCTCAAGTATCCATACGAAAATACTTAAAGTTTCTTGAAGATATACAGGTTCTGAATGTAGAAATGGCGTACGGAACGA
TCGGAAGACCTGTGTTTCAGTACAACGTCAACAACAGTAACATTAACGGAATAAAGCAATATCTATAA

Upstream 100 bases:

>100_bases
AGCATCGAAAATTTAAAAGGCCAAATGATCCTCACAAGCGAAAAGAATGAAGGAACGACATTCAGTATTCGCATCCCGTA
CGAACCGAAGGAGGAAAATC

Downstream 100 bases:

>100_bases
GAAAACGCACTGCTTGCTTTGAGCAGTGCGTTTTTCTGTCATATCTCTTGCCAAGATGCTTGATTCTATTGTTGATAAGT
TATACAAGAATAACAAAGGG

Product: two-component response regulator YufL

Products: NA

Alternate protein names: Malate response regulator

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MINVLIVEDDPMVGELNKRYLSQIDGFQLKGIASSFQSALHILGEHHIDLILLDIYMPGKNGLELLTELRAQNEAVDVIV
ISAASELDVIKKTLRYGAVDYLIKPFEFERFQTALSDYRRKQKVYSTHRNMSQKELDAELFQKKEATEKVQLPKGLTKST
LKLIWSSIQSFENESFTTEDLAKHTEISQVSIRKYLKFLEDIQVLNVEMAYGTIGRPVFQYNVNNSNINGIKQYL

Sequences:

>Translated_235_residues
MINVLIVEDDPMVGELNKRYLSQIDGFQLKGIASSFQSALHILGEHHIDLILLDIYMPGKNGLELLTELRAQNEAVDVIV
ISAASELDVIKKTLRYGAVDYLIKPFEFERFQTALSDYRRKQKVYSTHRNMSQKELDAELFQKKEATEKVQLPKGLTKST
LKLIWSSIQSFENESFTTEDLAKHTEISQVSIRKYLKFLEDIQVLNVEMAYGTIGRPVFQYNVNNSNINGIKQYL
>Mature_235_residues
MINVLIVEDDPMVGELNKRYLSQIDGFQLKGIASSFQSALHILGEHHIDLILLDIYMPGKNGLELLTELRAQNEAVDVIV
ISAASELDVIKKTLRYGAVDYLIKPFEFERFQTALSDYRRKQKVYSTHRNMSQKELDAELFQKKEATEKVQLPKGLTKST
LKLIWSSIQSFENESFTTEDLAKHTEISQVSIRKYLKFLEDIQVLNVEMAYGTIGRPVFQYNVNNSNINGIKQYL

Specific function: Member of a two-component regulatory system malK/malR. Activates transcription of maeA, maeN and yflS in presence of malate by binding to their promoter region

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1790566, Length=239, Percent_Identity=44.7698744769874, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI1786838, Length=217, Percent_Identity=30.8755760368664, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1788550, Length=137, Percent_Identity=33.5766423357664, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=104, Percent_Identity=35.5769230769231, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788222, Length=129, Percent_Identity=30.2325581395349, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787487, Length=104, Percent_Identity=36.5384615384615, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786784, Length=143, Percent_Identity=27.972027972028, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MALR_BACSU (O05251)

Other databases:

- EMBL:   Z93937
- EMBL:   AL009126
- PIR:   B70009
- RefSeq:   NP_391031.1
- ProteinModelPortal:   O05251
- SMR:   O05251
- EnsemblBacteria:   EBBACT00000000145
- GeneID:   937174
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31530
- NMPDR:   fig|224308.1.peg.3156
- GenoList:   BSU31530
- GeneTree:   EBGT00050000000045
- HOGENOM:   HBG753323
- OMA:   IKPFEFE
- PhylomeDB:   O05251
- ProtClustDB:   CLSK887796
- BioCyc:   BSUB:BSU31530-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR001789
- SMART:   SM00448

Pfam domain/function: PF00072 Response_reg; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 26965; Mature: 26965

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVLIVEDDPMVGELNKRYLSQIDGFQLKGIASSFQSALHILGEHHIDLILLDIYMPGK
CEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCC
NGLELLTELRAQNEAVDVIVISAASELDVIKKTLRYGAVDYLIKPFEFERFQTALSDYRR
CHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KQKVYSTHRNMSQKELDAELFQKKEATEKVQLPKGLTKSTLKLIWSSIQSFENESFTTED
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
LAKHTEISQVSIRKYLKFLEDIQVLNVEMAYGTIGRPVFQYNVNNSNINGIKQYL
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCCCHHHHHCC
>Mature Secondary Structure
MINVLIVEDDPMVGELNKRYLSQIDGFQLKGIASSFQSALHILGEHHIDLILLDIYMPGK
CEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCC
NGLELLTELRAQNEAVDVIVISAASELDVIKKTLRYGAVDYLIKPFEFERFQTALSDYRR
CHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KQKVYSTHRNMSQKELDAELFQKKEATEKVQLPKGLTKSTLKLIWSSIQSFENESFTTED
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
LAKHTEISQVSIRKYLKFLEDIQVLNVEMAYGTIGRPVFQYNVNNSNINGIKQYL
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160