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The map label for this gene is yufL

Identifier: 16080204

GI number: 16080204

Start: 3237157

End: 3238758

Strand: Direct

Name: yufL

Synonym: BSU31520

Alternate gene names: 16080204

Gene position: 3237157-3238758 (Clockwise)

Preceding gene: 16080202

Following gene: 16080205

Centisome position: 76.79

GC content: 44.26

Gene sequence:

>1602_bases
ATGAAAAAAACATTAAAACTGCAAACCAGACTCACCATATTTGTTTGTATCGTTGTATTAATCGCATTGCTCATTACGTT
TTTTACAGTCGGTGCCCAAACCACCAAACGAATTAGAGATCAGGAAAAGGCCACTGCGCTGCAGACTGCTGAAATGGTAG
CTGAAGCGCCGATGACAGCCGCTGCTTTAGAAAGCGGAAAAAAACAAAAGGAACTGCAGAGCTACACAAAGCGGGTTCAG
AAAATTACCGGCACGGAATTTGTTGTCGTCATGGATATGAACGGTATCCGCAAAACTCATCCTGACCCGAGCAAAATCGG
AAAAAAATTCAGAGGCGGTGATGAGTCCGAGGTATTAAAAGGGCATGTCCATATCAGCACAGCCTCTGGCACGCTCGGGA
AATCGCAGAGAGCGTTCGTCCCTGTCTACGCGGAGAATGGAAAACAGGTCGGTGCAGTCGCGGTCGGCATCACCGTAAAT
GAAATTGATGAAGTGATCAGCCATAGCTTACGGCCGCTTTACTTTATTATTTGCGTCAGCATCTTCGTCGGTGTGATCGG
AGCCGTTATCGTCGCCCGTACAGTGAAAAATATCATGTACGGACTTGAACCTTATGAAATTGCGACTCTGCTTGAAGAAC
GGAGCGCGATGCTGGAATCGACGAAGGAAGGAATACTCGCTGTCGATGAACATGGCAAAATTAAACTTGCCAACGCGGAA
GCAAAACGCCTATTCGTCAAAATGGGCATCAACACAAATCCCATTGATCAGGATGTAGATGACATCTTGCCGAAAAGCCG
CTTGAAAAAGGTCATTGAGACAAAAAAACCTCTTCAAGACAGAGACGTCCGCATTAACGGGCTGGAGCTTGTGTTTAATG
AAGTTCCTATCCAGCTGAAAGGACAAACTGTCGGAGCGATTGCAACATTCCGCGACAAGACCGAGGTAAAACATTTAGCT
GAACAGCTTTCCGGTGTTAAAATGTATGCAAACGCACTGAGAGCGCAGTCTCATGAATTTATGAACAAACTGCATGTGAT
ATTAGGTCTCGTCCAGCTGAAAGAGTATGATGATCTTGGAGATTATATTAAAGATATAGCCATACAGCAAAAATCAGAAA
CAAGTGAAATTATAAATGATGTCAAAAGCTCCGTACTGGCCGGTTTTCTGCTTGGAAAGCAAAGCTTTATACGTGAGCAG
GGCGCAAATCTTGATATTGAATGCAACGGTGTCATCCCGAACGCGGCAGACCCTTCTGTCATCCATGAACTTATTACAAT
CATCGGAAATCTCATCAACAATGGCTTAGATGCCGTTGCCGATATGCCGAAAAAACAAATCACCATGTCCATGCGCTTCC
ATAACAGCATACTTGATATTGAAATTACCGATACTGGAGCAGGCATGTCTGAAGAAGACCAAGCCAAAGTATTTGAACAA
GGCTATTCAACAAAGGGCAAAAACCGGGGATTCGGCCTTTATTTCACACAGCAAAGCATCGAAAATTTAAAAGGCCAAAT
GATCCTCACAAGCGAAAAGAATGAAGGAACGACATTCAGTATTCGCATCCCGTACGAACCGAAGGAGGAAAATCATGATT
AA

Upstream 100 bases:

>100_bases
TTTTTTCTCCTTTTTACGAATTACGGGCCCAAATCTTCACAAAGCAGCATTTTGAGAGTAAAGTAGTACGCAGGAATCAT
AAAACGTAAGGAAGTTTTAT

Downstream 100 bases:

>100_bases
TGTACTAATAGTTGAAGATGACCCCATGGTGGGTGAATTAAATAAACGATACTTAAGCCAAATAGATGGCTTTCAGCTGA
AAGGCATCGCTTCTTCCTTT

Product: sensory histidine kinase DcuS

Products: NA

Alternate protein names: Malate kinase sensor

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKKTLKLQTRLTIFVCIVVLIALLITFFTVGAQTTKRIRDQEKATALQTAEMVAEAPMTAAALESGKKQKELQSYTKRVQ
KITGTEFVVVMDMNGIRKTHPDPSKIGKKFRGGDESEVLKGHVHISTASGTLGKSQRAFVPVYAENGKQVGAVAVGITVN
EIDEVISHSLRPLYFIICVSIFVGVIGAVIVARTVKNIMYGLEPYEIATLLEERSAMLESTKEGILAVDEHGKIKLANAE
AKRLFVKMGINTNPIDQDVDDILPKSRLKKVIETKKPLQDRDVRINGLELVFNEVPIQLKGQTVGAIATFRDKTEVKHLA
EQLSGVKMYANALRAQSHEFMNKLHVILGLVQLKEYDDLGDYIKDIAIQQKSETSEIINDVKSSVLAGFLLGKQSFIREQ
GANLDIECNGVIPNAADPSVIHELITIIGNLINNGLDAVADMPKKQITMSMRFHNSILDIEITDTGAGMSEEDQAKVFEQ
GYSTKGKNRGFGLYFTQQSIENLKGQMILTSEKNEGTTFSIRIPYEPKEENHD

Sequences:

>Translated_533_residues
MKKTLKLQTRLTIFVCIVVLIALLITFFTVGAQTTKRIRDQEKATALQTAEMVAEAPMTAAALESGKKQKELQSYTKRVQ
KITGTEFVVVMDMNGIRKTHPDPSKIGKKFRGGDESEVLKGHVHISTASGTLGKSQRAFVPVYAENGKQVGAVAVGITVN
EIDEVISHSLRPLYFIICVSIFVGVIGAVIVARTVKNIMYGLEPYEIATLLEERSAMLESTKEGILAVDEHGKIKLANAE
AKRLFVKMGINTNPIDQDVDDILPKSRLKKVIETKKPLQDRDVRINGLELVFNEVPIQLKGQTVGAIATFRDKTEVKHLA
EQLSGVKMYANALRAQSHEFMNKLHVILGLVQLKEYDDLGDYIKDIAIQQKSETSEIINDVKSSVLAGFLLGKQSFIREQ
GANLDIECNGVIPNAADPSVIHELITIIGNLINNGLDAVADMPKKQITMSMRFHNSILDIEITDTGAGMSEEDQAKVFEQ
GYSTKGKNRGFGLYFTQQSIENLKGQMILTSEKNEGTTFSIRIPYEPKEENHD
>Mature_533_residues
MKKTLKLQTRLTIFVCIVVLIALLITFFTVGAQTTKRIRDQEKATALQTAEMVAEAPMTAAALESGKKQKELQSYTKRVQ
KITGTEFVVVMDMNGIRKTHPDPSKIGKKFRGGDESEVLKGHVHISTASGTLGKSQRAFVPVYAENGKQVGAVAVGITVN
EIDEVISHSLRPLYFIICVSIFVGVIGAVIVARTVKNIMYGLEPYEIATLLEERSAMLESTKEGILAVDEHGKIKLANAE
AKRLFVKMGINTNPIDQDVDDILPKSRLKKVIETKKPLQDRDVRINGLELVFNEVPIQLKGQTVGAIATFRDKTEVKHLA
EQLSGVKMYANALRAQSHEFMNKLHVILGLVQLKEYDDLGDYIKDIAIQQKSETSEIINDVKSSVLAGFLLGKQSFIREQ
GANLDIECNGVIPNAADPSVIHELITIIGNLINNGLDAVADMPKKQITMSMRFHNSILDIEITDTGAGMSEEDQAKVFEQ
GYSTKGKNRGFGLYFTQQSIENLKGQMILTSEKNEGTTFSIRIPYEPKEENHD

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain

Homologues:

Organism=Escherichia coli, GI1790567, Length=538, Percent_Identity=35.1301115241636, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI1786837, Length=540, Percent_Identity=28.5185185185185, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1788549, Length=378, Percent_Identity=21.6931216931217, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MALK_BACSU (O05250)

Other databases:

- EMBL:   Z93937
- EMBL:   AL009126
- PIR:   A70009
- RefSeq:   NP_391030.1
- ProteinModelPortal:   O05250
- EnsemblBacteria:   EBBACT00000001973
- GeneID:   937173
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31520
- NMPDR:   fig|224308.1.peg.3155
- GenoList:   BSU31520
- GeneTree:   EBGT00050000000017
- HOGENOM:   HBG537380
- OMA:   AQTHEFM
- PhylomeDB:   O05250
- ProtClustDB:   PRK11086
- BioCyc:   BSUB:BSU31520-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:1.10.287.130
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00091

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS; SSF55874 ATP_bd_ATPase; SSF55890 Sensor_kinase_SpoOB-type

EC number: =2.7.13.3

Molecular weight: Translated: 58931; Mature: 58931

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS50109 HIS_KIN; PS50112 PAS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1205f4e8)-; HASH(0x11ea6f2c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLKLQTRLTIFVCIVVLIALLITFFTVGAQTTKRIRDQEKATALQTAEMVAEAPMTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
AALESGKKQKELQSYTKRVQKITGTEFVVVMDMNGIRKTHPDPSKIGKKFRGGDESEVLK
HHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHEEE
GHVHISTASGTLGKSQRAFVPVYAENGKQVGAVAVGITVNEIDEVISHSLRPLYFIICVS
EEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCHHHHHHHHH
IFVGVIGAVIVARTVKNIMYGLEPYEIATLLEERSAMLESTKEGILAVDEHGKIKLANAE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCH
AKRLFVKMGINTNPIDQDVDDILPKSRLKKVIETKKPLQDRDVRINGLELVFNEVPIQLK
HHEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCEEECHHEEEEECCCEEEC
GQTVGAIATFRDKTEVKHLAEQLSGVKMYANALRAQSHEFMNKLHVILGLVQLKEYDDLG
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYIKDIAIQQKSETSEIINDVKSSVLAGFLLGKQSFIREQGANLDIECNGVIPNAADPSV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHH
IHELITIIGNLINNGLDAVADMPKKQITMSMRFHNSILDIEITDTGAGMSEEDQAKVFEQ
HHHHHHHHHHHHHCCHHHHHHCCHHHHEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHH
GYSTKGKNRGFGLYFTQQSIENLKGQMILTSEKNEGTTFSIRIPYEPKEENHD
CCCCCCCCCCEEEEEEHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCCCCH
>Mature Secondary Structure
MKKTLKLQTRLTIFVCIVVLIALLITFFTVGAQTTKRIRDQEKATALQTAEMVAEAPMTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
AALESGKKQKELQSYTKRVQKITGTEFVVVMDMNGIRKTHPDPSKIGKKFRGGDESEVLK
HHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHEEE
GHVHISTASGTLGKSQRAFVPVYAENGKQVGAVAVGITVNEIDEVISHSLRPLYFIICVS
EEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCHHHHHHHHH
IFVGVIGAVIVARTVKNIMYGLEPYEIATLLEERSAMLESTKEGILAVDEHGKIKLANAE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCH
AKRLFVKMGINTNPIDQDVDDILPKSRLKKVIETKKPLQDRDVRINGLELVFNEVPIQLK
HHEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCEEECHHEEEEECCCEEEC
GQTVGAIATFRDKTEVKHLAEQLSGVKMYANALRAQSHEFMNKLHVILGLVQLKEYDDLG
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYIKDIAIQQKSETSEIINDVKSSVLAGFLLGKQSFIREQGANLDIECNGVIPNAADPSV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHH
IHELITIIGNLINNGLDAVADMPKKQITMSMRFHNSILDIEITDTGAGMSEEDQAKVFEQ
HHHHHHHHHHHHHCCHHHHHHCCHHHHEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHH
GYSTKGKNRGFGLYFTQQSIENLKGQMILTSEKNEGTTFSIRIPYEPKEENHD
CCCCCCCCCCEEEEEEHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160