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The map label for this gene is pbuO

Identifier: 16080051

GI number: 16080051

Start: 3068908

End: 3070206

Strand: Direct

Name: pbuO

Synonym: BSU29990

Alternate gene names: 16080051

Gene position: 3068908-3070206 (Clockwise)

Preceding gene: 16080049

Following gene: 16080054

Centisome position: 72.8

GC content: 48.5

Gene sequence:

>1299_bases
ATGTTTCATCTAAAAGAGCAGCAAACATCCATAAAGCAAGAAATCATAGCAGGGCTGACGACCTTTTTCACAATGGTCTA
TATCGTCGTGGTCAATCCGGTCATTCTGGCTAACGCCGGGGTTCCTTTCGACCAGGTTTTCACCGCAACGATTATTGCCT
CCATTGTCGGAACGCTGTGGATGGCCCTGGCAGCAAATTATCCGATTGCTATTGCGCCCGGCATGGGCTTGAACGCTTAT
TTAGCCTTTCATGTCGTAAGCGCAAGCGATGGCGGCATCACGTACGCAACAGCTTTCAGCGCAGTATTTACAGCAGGGGT
TCTCTTCATTATTCTATCGTTAACGCCTTTAAGAAAACAACTTATAGAAGCGATTCCAAACAATTTAAAATACGGGATCA
CAACAGGGATCGGGCTGTTTATTGCTTTTATCGGTTTGCGTCAAGCGGGAATTGTGGCAGCTGATGAGTCAAACCTGGTC
ACTCTCGGCAATCTGCATTCACCTGGTGTTATCTTAACGCTGGTCGGCCTTTTGATCAGCGTGGTCCTGATGGTGCTGAA
TGTAAGCGGCGCTTTATTTATCGGAATGGCAGCTACCGCTTTGATCGCCTTTTTCACCGGTCAGCTCCATTTTTCAAAGG
GATTTATGTCGCTTCCTCATTTGCCGGAAGGATTGATGATCTCAAATCCATTTACCGCTTTTGGCGATGTCATTCATCAC
GGCCTGTACGCTGTCGTCTTTTCTTTTCTATTAGTCACCATTTTTGATACGACAGGCACGATGATCGGAGTTGCCGAGCA
AGCCGGGCTGATGAAAAACAACAAGCTGCCGAACGTGCGAAAAGCGCTGCTTGCTGATTCAACCGCGACGACAGTAGGAG
CTGTTTTCGGCACAAGTCCGACGACTGCATTTATCGAGTCTTCCGCCGGTGTCGCAGCCGGGGGAAGAACGGGGCTTACG
GCTTTGACTGTCGCGGTGATGTTTGCGGCTTCAATGTTTTTCAGTCCGCTTGTCAGCGCCTTGTCAGGTATAGCCGCCAT
CACCTCGCCTGCTTTGATTATCGTCGGCAGCCTGATGATGGGCTCAGTTTCCAATATGAACTGGAAAGAGATGGACGAGG
CATTTCCTGCGTTTCTCGTCATTCTAGCCATGCCTTTGACCTCGAGCATTTCTACAGGAATCGCGCTTGGCTTTATTTCG
TACCCAATTGTAAAAGCGGCGCGGGGAAAATGGAGAGAGATTCATCCGCTCGTCATCGTTTTCGCCATTCTGTTTTTCAT
CCAGCTGTTTATTTTATAA

Upstream 100 bases:

>100_bases
TCATAATGATCCCCTTTCCGCAAGAAATGACTTGCAAAAACCAAATAAAACGAATAATATTAATGGTGTTTTGTTAAAAC
GTTCGTAATTGGAGGATACT

Downstream 100 bases:

>100_bases
GGATAGACCAAAAAACCCAAACGTCGCCGTTTGGGTTTTTTATGTAAACAATAAAGAGACAGCGGCCGCTAAAAAACAAC
AGGCGGCAAACGCATAGTGC

Product: hypoxanthine/guanine permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MFHLKEQQTSIKQEIIAGLTTFFTMVYIVVVNPVILANAGVPFDQVFTATIIASIVGTLWMALAANYPIAIAPGMGLNAY
LAFHVVSASDGGITYATAFSAVFTAGVLFIILSLTPLRKQLIEAIPNNLKYGITTGIGLFIAFIGLRQAGIVAADESNLV
TLGNLHSPGVILTLVGLLISVVLMVLNVSGALFIGMAATALIAFFTGQLHFSKGFMSLPHLPEGLMISNPFTAFGDVIHH
GLYAVVFSFLLVTIFDTTGTMIGVAEQAGLMKNNKLPNVRKALLADSTATTVGAVFGTSPTTAFIESSAGVAAGGRTGLT
ALTVAVMFAASMFFSPLVSALSGIAAITSPALIIVGSLMMGSVSNMNWKEMDEAFPAFLVILAMPLTSSISTGIALGFIS
YPIVKAARGKWREIHPLVIVFAILFFIQLFIL

Sequences:

>Translated_432_residues
MFHLKEQQTSIKQEIIAGLTTFFTMVYIVVVNPVILANAGVPFDQVFTATIIASIVGTLWMALAANYPIAIAPGMGLNAY
LAFHVVSASDGGITYATAFSAVFTAGVLFIILSLTPLRKQLIEAIPNNLKYGITTGIGLFIAFIGLRQAGIVAADESNLV
TLGNLHSPGVILTLVGLLISVVLMVLNVSGALFIGMAATALIAFFTGQLHFSKGFMSLPHLPEGLMISNPFTAFGDVIHH
GLYAVVFSFLLVTIFDTTGTMIGVAEQAGLMKNNKLPNVRKALLADSTATTVGAVFGTSPTTAFIESSAGVAAGGRTGLT
ALTVAVMFAASMFFSPLVSALSGIAAITSPALIIVGSLMMGSVSNMNWKEMDEAFPAFLVILAMPLTSSISTGIALGFIS
YPIVKAARGKWREIHPLVIVFAILFFIQLFIL
>Mature_432_residues
MFHLKEQQTSIKQEIIAGLTTFFTMVYIVVVNPVILANAGVPFDQVFTATIIASIVGTLWMALAANYPIAIAPGMGLNAY
LAFHVVSASDGGITYATAFSAVFTAGVLFIILSLTPLRKQLIEAIPNNLKYGITTGIGLFIAFIGLRQAGIVAADESNLV
TLGNLHSPGVILTLVGLLISVVLMVLNVSGALFIGMAATALIAFFTGQLHFSKGFMSLPHLPEGLMISNPFTAFGDVIHH
GLYAVVFSFLLVTIFDTTGTMIGVAEQAGLMKNNKLPNVRKALLADSTATTVGAVFGTSPTTAFIESSAGVAAGGRTGLT
ALTVAVMFAASMFFSPLVSALSGIAAITSPALIIVGSLMMGSVSNMNWKEMDEAFPAFLVILAMPLTSSISTGIALGFIS
YPIVKAARGKWREIHPLVIVFAILFFIQLFIL

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine. May work at purine concentrations higher than 100 uM

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily

Homologues:

Organism=Escherichia coli, GI87082309, Length=438, Percent_Identity=43.6073059360731, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1790150, Length=430, Percent_Identity=41.8604651162791, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1790499, Length=433, Percent_Identity=35.1039260969977, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI48994909, Length=420, Percent_Identity=33.8095238095238, Blast_Score=197, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBUO_BACSU (O34978)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   G69993
- RefSeq:   NP_390877.1
- ProteinModelPortal:   O34978
- EnsemblBacteria:   EBBACT00000000573
- GeneID:   938070
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29990
- NMPDR:   fig|224308.1.peg.3002
- GenoList:   BSU29990
- GeneTree:   EBGT00050000001003
- HOGENOM:   HBG750189
- OMA:   NAYLAFH
- PhylomeDB:   O34978
- ProtClustDB:   CLSK887737
- BioCyc:   BSUB:BSU29990-MONOMER
- InterPro:   IPR006043
- PANTHER:   PTHR11119

Pfam domain/function: PF00860 Xan_ur_permease

EC number: NA

Molecular weight: Translated: 45421; Mature: 45421

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x111ba01c)-; HASH(0xfdacdf4)-; HASH(0x116e8af8)-; HASH(0x11a9d5ec)-; HASH(0x11a69268)-; HASH(0x11b19c90)-; HASH(0x11c6c21c)-; HASH(0x11600a84)-; HASH(0xf6ee320)-; HASH(0x11853bfc)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHLKEQQTSIKQEIIAGLTTFFTMVYIVVVNPVILANAGVPFDQVFTATIIASIVGTLW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCHHHHHHHHHHHHHHHHHH
MALAANYPIAIAPGMGLNAYLAFHVVSASDGGITYATAFSAVFTAGVLFIILSLTPLRKQ
HHHHCCCCEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LIEAIPNNLKYGITTGIGLFIAFIGLRQAGIVAADESNLVTLGNLHSPGVILTLVGLLIS
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCHHHHHHHHHHHH
VVLMVLNVSGALFIGMAATALIAFFTGQLHFSKGFMSLPHLPEGLMISNPFTAFGDVIHH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHH
GLYAVVFSFLLVTIFDTTGTMIGVAEQAGLMKNNKLPNVRKALLADSTATTVGAVFGTSP
HHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCC
TTAFIESSAGVAAGGRTGLTALTVAVMFAASMFFSPLVSALSGIAAITSPALIIVGSLMM
CHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GSVSNMNWKEMDEAFPAFLVILAMPLTSSISTGIALGFISYPIVKAARGKWREIHPLVIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FAILFFIQLFIL
HHHHHHHHHHHH
>Mature Secondary Structure
MFHLKEQQTSIKQEIIAGLTTFFTMVYIVVVNPVILANAGVPFDQVFTATIIASIVGTLW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCHHHHHHHHHHHHHHHHHH
MALAANYPIAIAPGMGLNAYLAFHVVSASDGGITYATAFSAVFTAGVLFIILSLTPLRKQ
HHHHCCCCEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LIEAIPNNLKYGITTGIGLFIAFIGLRQAGIVAADESNLVTLGNLHSPGVILTLVGLLIS
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCHHHHHHHHHHHH
VVLMVLNVSGALFIGMAATALIAFFTGQLHFSKGFMSLPHLPEGLMISNPFTAFGDVIHH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHH
GLYAVVFSFLLVTIFDTTGTMIGVAEQAGLMKNNKLPNVRKALLADSTATTVGAVFGTSP
HHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCC
TTAFIESSAGVAAGGRTGLTALTVAVMFAASMFFSPLVSALSGIAAITSPALIIVGSLMM
CHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GSVSNMNWKEMDEAFPAFLVILAMPLTSSISTGIALGFISYPIVKAARGKWREIHPLVIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FAILFFIQLFIL
HHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 11591660