BacMap

Definition	Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession	NC_000964
Length	4,215,606

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The map label for this gene is ytkP

Identifier: 16080049

GI number: 16080049

Start: 3066451

End: 3067386

Strand: Direct

Name: ytkP

Synonym: BSU29970

Alternate gene names: 16080049

Gene position: 3066451-3067386 (Clockwise)

Preceding gene: 16080043

Following gene: 16080051

Centisome position: 72.74

GC content: 47.54

Gene sequence:

>936_bases
ATGTTGAAAATCGTTGCAAACACAGCTGATCTGATAGGAGATACACCGCTAGTGAGATTGAATCGGCTTCAGCCGGAAAA
TGCGGCACAAGTCTATTTAAAGCTTGAATTTTTCAATCCGAGCGGCAGTGTGAAAGACAGAGCCGCTTATCAAATGATCA
TAGAAGCTGAACAAAACGGTTTACTCAAGCCGGGATCAGTTATTATAGAGCCGACAAGCGGAAATACCGGCATCGGGCTC
GCCATGAATGCGGCAGCACGCGGATATAAAGCAATCTTAGTTATGCCTGATACGATGACGAAGGAACGGATCAACCTACT
TAAAGCTTACGGTGCAGAGGTTGTATTAACTCCCGGAGAGGAAAGAATGCCGGGCAGCATCAAAAAAGCGAAGGAGCTTG
CCGAACAAATTCCGAACAGCTATATTCCAATGCAGTTCGATAACACTGCTAATCCTGATGCGCACCGAAAAACAACAGCG
CCTGAAATCGCCCGGGCAATAGAGGAAATCGGAAAACCGCTCGGCGCTTTTGTAGCCTCCTCTGGAACGGGCGGAACCAT
TACCGGAACAGGTGAGGCGCTCAAAGAGCTTTTTCCGGATATCACGGTTCATGTTGTTGAGCCAGCGGGTTCGCCTGTAT
TATCCGGCGGAAAACCTGGCGCGCATAAACTTGTAGGAACGAGCCCAGGCTTTATACCGCCGATTTTAAATGAAGACGTT
TATGATGAAATTATCAAAATCTCTGATGAAGACGCGTATACGACGACAAGACGGCTTGCTGCTGAAGAAGGAATTCTTGT
CGGTCCTTCATCAGGCGCTGCCTGCTTCGCGGCAATCGAAACAGCGAAACGCCTCTCTCCTGACAAAGTTGTGGTCTGCA
TGACAGCTGATACAGGAGAACGGTACTTATCAACTGATTTATGGTCATTTATTTAA

Upstream 100 bases:

>100_bases
GCTGTGCGGATAGAGGAACAGTGTATGAAAACACTCTCGCACAAACGTTTATTATGTTAGGATATTATATGACACTTGTT
TTATTCAGAAAGGAATGACA

Downstream 100 bases:

>100_bases
AAAAAGGACTGGCTTCAGCAGAAGCCGGTCCTTTTATTTTGCAAGCTCGTAAATGGCCTGAGCATATAACGCTGTCGATC
TCAGCAGGTCATCAATTTCG

Product: cysteine synthase-like protein

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MLKIVANTADLIGDTPLVRLNRLQPENAAQVYLKLEFFNPSGSVKDRAAYQMIIEAEQNGLLKPGSVIIEPTSGNTGIGL
AMNAAARGYKAILVMPDTMTKERINLLKAYGAEVVLTPGEERMPGSIKKAKELAEQIPNSYIPMQFDNTANPDAHRKTTA
PEIARAIEEIGKPLGAFVASSGTGGTITGTGEALKELFPDITVHVVEPAGSPVLSGGKPGAHKLVGTSPGFIPPILNEDV
YDEIIKISDEDAYTTTRRLAAEEGILVGPSSGAACFAAIETAKRLSPDKVVVCMTADTGERYLSTDLWSFI

Sequences:

>Translated_311_residues
MLKIVANTADLIGDTPLVRLNRLQPENAAQVYLKLEFFNPSGSVKDRAAYQMIIEAEQNGLLKPGSVIIEPTSGNTGIGL
AMNAAARGYKAILVMPDTMTKERINLLKAYGAEVVLTPGEERMPGSIKKAKELAEQIPNSYIPMQFDNTANPDAHRKTTA
PEIARAIEEIGKPLGAFVASSGTGGTITGTGEALKELFPDITVHVVEPAGSPVLSGGKPGAHKLVGTSPGFIPPILNEDV
YDEIIKISDEDAYTTTRRLAAEEGILVGPSSGAACFAAIETAKRLSPDKVVVCMTADTGERYLSTDLWSFI
>Mature_311_residues
MLKIVANTADLIGDTPLVRLNRLQPENAAQVYLKLEFFNPSGSVKDRAAYQMIIEAEQNGLLKPGSVIIEPTSGNTGIGL
AMNAAARGYKAILVMPDTMTKERINLLKAYGAEVVLTPGEERMPGSIKKAKELAEQIPNSYIPMQFDNTANPDAHRKTTA
PEIARAIEEIGKPLGAFVASSGTGGTITGTGEALKELFPDITVHVVEPAGSPVLSGGKPGAHKLVGTSPGFIPPILNEDV
YDEIIKISDEDAYTTTRRLAAEEGILVGPSSGAACFAAIETAKRLSPDKVVVCMTADTGERYLSTDLWSFI

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family

Homologues:

Organism=Homo sapiens, GI295821202, Length=319, Percent_Identity=41.692789968652, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI295821200, Length=319, Percent_Identity=41.692789968652, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI4557415, Length=319, Percent_Identity=41.692789968652, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI11345492, Length=319, Percent_Identity=22.884012539185, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI2367138, Length=298, Percent_Identity=45.3020134228188, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI1788754, Length=319, Percent_Identity=46.7084639498433, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17535051, Length=308, Percent_Identity=46.4285714285714, Blast_Score=251, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17562970, Length=308, Percent_Identity=45.4545454545455, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI32566674, Length=302, Percent_Identity=45.6953642384106, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI115535073, Length=308, Percent_Identity=45.7792207792208, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17561720, Length=310, Percent_Identity=43.2258064516129, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI32566672, Length=216, Percent_Identity=43.9814814814815, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17534315, Length=325, Percent_Identity=37.8461538461538, Blast_Score=158, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI25147552, Length=322, Percent_Identity=36.3354037267081, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17561716, Length=113, Percent_Identity=42.4778761061947, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71996324, Length=331, Percent_Identity=25.3776435045317, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=322, Percent_Identity=38.8198757763975, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6321449, Length=331, Percent_Identity=34.441087613293, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24643623, Length=319, Percent_Identity=40.4388714733542, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI20129101, Length=319, Percent_Identity=40.4388714733542, Blast_Score=209, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSM_BACSU (O34476)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   H69994
- RefSeq:   NP_390875.1
- ProteinModelPortal:   O34476
- SMR:   O34476
- EnsemblBacteria:   EBBACT00000002566
- GeneID:   936733
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29970
- NMPDR:   fig|224308.1.peg.3000
- GenoList:   BSU29970
- GeneTree:   EBGT00070000031817
- HOGENOM:   HBG748215
- OMA:   CIAPDTG
- PhylomeDB:   O34476
- ProtClustDB:   CLSK873562
- BioCyc:   BSUB:BSU29970-MONOMER
- BRENDA:   2.5.1.47
- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926
- TIGRFAMs:   TIGR01139
- TIGRFAMs:   TIGR01136

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =2.5.1.47

Molecular weight: Translated: 33066; Mature: 33066

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: BINDING 75-75 BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIVANTADLIGDTPLVRLNRLQPENAAQVYLKLEFFNPSGSVKDRAAYQMIIEAEQNG
CEEEEECCHHHHCCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCHHHHHEEEEECCCCC
LLKPGSVIIEPTSGNTGIGLAMNAAARGYKAILVMPDTMTKERINLLKAYGAEVVLTPGE
CCCCCCEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEECCCC
ERMPGSIKKAKELAEQIPNSYIPMQFDNTANPDAHRKTTAPEIARAIEEIGKPLGAFVAS
CCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCC
SGTGGTITGTGEALKELFPDITVHVVEPAGSPVLSGGKPGAHKLVGTSPGFIPPILNEDV
CCCCCEEECCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHH
YDEIIKISDEDAYTTTRRLAAEEGILVGPSSGAACFAAIETAKRLSPDKVVVCMTADTGE
HHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
RYLSTDLWSFI
HHHHHHHHHCC
>Mature Secondary Structure
MLKIVANTADLIGDTPLVRLNRLQPENAAQVYLKLEFFNPSGSVKDRAAYQMIIEAEQNG
CEEEEECCHHHHCCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCHHHHHEEEEECCCCC
LLKPGSVIIEPTSGNTGIGLAMNAAARGYKAILVMPDTMTKERINLLKAYGAEVVLTPGE
CCCCCCEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEECCCC
ERMPGSIKKAKELAEQIPNSYIPMQFDNTANPDAHRKTTAPEIARAIEEIGKPLGAFVAS
CCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCC
SGTGGTITGTGEALKELFPDITVHVVEPAGSPVLSGGKPGAHKLVGTSPGFIPPILNEDV
CCCCCEEECCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHH
YDEIIKISDEDAYTTTRRLAAEEGILVGPSSGAACFAAIETAKRLSPDKVVVCMTADTGE
HHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
RYLSTDLWSFI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377