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The map label for this gene is dnaI

Identifier: 16079950

GI number: 16079950

Start: 2963185

End: 2964120

Strand: Reverse

Name: dnaI

Synonym: BSU28980

Alternate gene names: 16079950

Gene position: 2964120-2963185 (Counterclockwise)

Preceding gene: 16079951

Following gene: 16079949

Centisome position: 70.31

GC content: 43.06

Gene sequence:

>936_bases
ATGGAACCAATCGGCCGTTCCCTGCAGGGCGTAACCGGCAGGCCGGATTTCCAAAAACGTCTTGAACAAATGAAGGAAAA
AGTCATGAAAGATCAAGATGTTCAAGCATTTTTGAAGGAAAACGAAGAAGTGATTGACCAAAAAATGATCGAAAAGAGCT
TAAATAAGCTTTATGAATATATCGAACAAAGCAAGAATTGCTCCTATTGTTCGGAAGATGAAAACTGCAACAATTTGTTG
GAGGGCTACCATCCGAAGCTTGTTGTCAATGGGAGGTCTATTGATATCGAGTATTACGAATGTCCAGTCAAACGGAAGCT
CGACCAGCAGAAGAAACAACAGTCTCTTATGAAAAGCATGTATATCCAGCAGGATCTTCTTGGAGCGACGTTCCAGCAAG
TTGATATCAGTGACCCAAGCAGACTCGCGATGTTTCAGCATGTTACAGACTTCCTGAAAAGCTATAATGAGACGGGAAAA
GGGAAAGGATTATATTTATACGGGAAATTTGGGGTAGGAAAAACGTTTATGCTCGCTGCAATTGCCAATGAGCTGGCGGA
AAAAGAGTATTCCTCCATGATTGTTTATGTGCCTGAATTTGTGAGAGAGCTTAAGAATTCGCTGCAAGACCAGACATTGG
AAGAGAAGCTCAATATGGTAAAGACCACACCCGTATTAATGCTTGATGACATCGGAGCAGAATCAATGACAAGCTGGGTA
AGGGATGAGGTCATCGGAACGGTGCTTCAGCACAGAATGTCCCAGCAGCTGCCAACGTTCTTTTCTTCTAATTTCTCACC
TGACGAGCTGAAGCATCATTTTACGTATTCGCAGAGGGGAGAAAAAGAAGAAGTAAAAGCTGCAAGATTAATGGAGCGGA
TATTATATTTGGCTGCTCCGATCCGCCTTGACGGAGAAAACCGCCGACATCCATAA

Upstream 100 bases:

>100_bases
GCAGGATTTGGAAGAACAGAAGAAAAAGATGATGGAAGAAATGCAAAAACTGAAAAAATACTCTGCCTATTAAAGACAGA
GATGCGGGGTGAAGGAATAG

Downstream 100 bases:

>100_bases
TTGCTGTGGTTCAAAGGGGAAGGAACTGAGGGTATGAAACGAAAAACGGCTGTCAAATGGCTGGCAGTGCTTGCAGGTGC
TGGTTTACTCTATTGGGGAA

Product: primosomal protein DnaI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MEPIGRSLQGVTGRPDFQKRLEQMKEKVMKDQDVQAFLKENEEVIDQKMIEKSLNKLYEYIEQSKNCSYCSEDENCNNLL
EGYHPKLVVNGRSIDIEYYECPVKRKLDQQKKQQSLMKSMYIQQDLLGATFQQVDISDPSRLAMFQHVTDFLKSYNETGK
GKGLYLYGKFGVGKTFMLAAIANELAEKEYSSMIVYVPEFVRELKNSLQDQTLEEKLNMVKTTPVLMLDDIGAESMTSWV
RDEVIGTVLQHRMSQQLPTFFSSNFSPDELKHHFTYSQRGEKEEVKAARLMERILYLAAPIRLDGENRRHP

Sequences:

>Translated_311_residues
MEPIGRSLQGVTGRPDFQKRLEQMKEKVMKDQDVQAFLKENEEVIDQKMIEKSLNKLYEYIEQSKNCSYCSEDENCNNLL
EGYHPKLVVNGRSIDIEYYECPVKRKLDQQKKQQSLMKSMYIQQDLLGATFQQVDISDPSRLAMFQHVTDFLKSYNETGK
GKGLYLYGKFGVGKTFMLAAIANELAEKEYSSMIVYVPEFVRELKNSLQDQTLEEKLNMVKTTPVLMLDDIGAESMTSWV
RDEVIGTVLQHRMSQQLPTFFSSNFSPDELKHHFTYSQRGEKEEVKAARLMERILYLAAPIRLDGENRRHP
>Mature_311_residues
MEPIGRSLQGVTGRPDFQKRLEQMKEKVMKDQDVQAFLKENEEVIDQKMIEKSLNKLYEYIEQSKNCSYCSEDENCNNLL
EGYHPKLVVNGRSIDIEYYECPVKRKLDQQKKQQSLMKSMYIQQDLLGATFQQVDISDPSRLAMFQHVTDFLKSYNETGK
GKGLYLYGKFGVGKTFMLAAIANELAEKEYSSMIVYVPEFVRELKNSLQDQTLEEKLNMVKTTPVLMLDDIGAESMTSWV
RDEVIGTVLQHRMSQQLPTFFSSNFSPDELKHHFTYSQRGEKEEVKAARLMERILYLAAPIRLDGENRRHP

Specific function: Probably involved in DNA replication

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAI_BACSU (P06567)

Other databases:

- EMBL:   X04963
- EMBL:   Z75208
- EMBL:   AF008220
- EMBL:   AL009126
- EMBL:   M15183
- PIR:   B24720
- RefSeq:   NP_390776.1
- PDB:   2K7R
- PDBsum:   2K7R
- ProteinModelPortal:   P06567
- SMR:   P06567
- EnsemblBacteria:   EBBACT00000001390
- GeneID:   936873
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28980
- NMPDR:   fig|224308.1.peg.2901
- GenoList:   BSU28980
- GeneTree:   EBGT00070000032007
- HOGENOM:   HBG341598
- OMA:   KTYILAG
- ProtClustDB:   PRK08939
- BioCyc:   BSUB:BSU28980-MONOMER
- InterPro:   IPR003593
- InterPro:   IPR009928
- InterPro:   IPR002611
- SMART:   SM00382

Pfam domain/function: PF07319 DnaI_N; PF01695 IstB

EC number: NA

Molecular weight: Translated: 36115; Mature: 36115

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIGRSLQGVTGRPDFQKRLEQMKEKVMKDQDVQAFLKENEEVIDQKMIEKSLNKLYEY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IEQSKNCSYCSEDENCNNLLEGYHPKLVVNGRSIDIEYYECPVKRKLDQQKKQQSLMKSM
HHHCCCCCCCCCCCCHHHHHHCCCCEEEECCCEEEEEHHHCCHHHHHHHHHHHHHHHHHH
YIQQDLLGATFQQVDISDPSRLAMFQHVTDFLKSYNETGKGKGLYLYGKFGVGKTFMLAA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHH
IANELAEKEYSSMIVYVPEFVRELKNSLQDQTLEEKLNMVKTTPVLMLDDIGAESMTSWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHH
RDEVIGTVLQHRMSQQLPTFFSSNFSPDELKHHFTYSQRGEKEEVKAARLMERILYLAAP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHCC
IRLDGENRRHP
CEECCCCCCCC
>Mature Secondary Structure
MEPIGRSLQGVTGRPDFQKRLEQMKEKVMKDQDVQAFLKENEEVIDQKMIEKSLNKLYEY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IEQSKNCSYCSEDENCNNLLEGYHPKLVVNGRSIDIEYYECPVKRKLDQQKKQQSLMKSM
HHHCCCCCCCCCCCCHHHHHHCCCCEEEECCCEEEEEHHHCCHHHHHHHHHHHHHHHHHH
YIQQDLLGATFQQVDISDPSRLAMFQHVTDFLKSYNETGKGKGLYLYGKFGVGKTFMLAA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHH
IANELAEKEYSSMIVYVPEFVRELKNSLQDQTLEEKLNMVKTTPVLMLDDIGAESMTSWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHH
RDEVIGTVLQHRMSQQLPTFFSSNFSPDELKHHFTYSQRGEKEEVKAARLMERILYLAAP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHCC
IRLDGENRRHP
CEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3027671; 8969504; 9387221; 9384377; 3027697; 1549481; 7773414