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The map label for this gene is thrS

Identifier: 16079947

GI number: 16079947

Start: 2959257

End: 2961188

Strand: Reverse

Name: thrS

Synonym: BSU28950

Alternate gene names: 16079947

Gene position: 2961188-2959257 (Counterclockwise)

Preceding gene: 255767668

Following gene: 16079945

Centisome position: 70.24

GC content: 46.38

Gene sequence:

>1932_bases
ATGTCAGATATGGTAAAAATCACATTTCCTGATGGAGCAGTCAAGGAGTTTGCGAAAGGAACAACAACAGAAGATATCGC
GGCATCCATCAGTCCGGGATTAAAGAAAAAGTCATTAGCCGGAAAACTGAACGGAAAAGAAATCGATTTGAGAACGCCGA
TCAATGAAGACGGTACAGTGGAAATCATTACAGAAGGCTCAGAAGAAGGTCTTCAAATTATGCGCCACAGTGCGGCTCAC
TTGCTGGCTCAAGCGATTAAACGCATCTACAAGGATGTTAAATTCGGCGTCGGTCCGGTTATCGAAAACGGTTTTTACTA
CGATGTAGAAATGGACGAAGCGATTACACCGGAGGATCTGCCGAAAATCGAGAAAGAAATGAAAAAAATCGTTAATGCGA
ACCTTCCGATCGTTCGAAAAGAAGTCAGCCGTGAAGAAGCGAAAGCCCGTTTTGCGGAAATCGGCGACGACCTGAAGCTT
GAACTATTGGATGCGATTCCTGAAGGAGAAACCGTTTCGATCTATGAGCAAGGCGAATTCTTTGACCTGTGCCGCGGCGT
CCATGTTCCTTCAACCGGAAAAATCAAAGAATTTAAGCTGTTAAGCCTTGCAGGCGCATACTGGCGCGGTGACAGCAAAA
ACCAAATGCTTCAGCGCGTATATGGTACAGCTTTCTTCAAAAAAGCTGATCTTGAAGAGCATCTTCGTATGCTAGAAGAA
GCGAAAGAACGCGACCACAGAAAGCTTGGCAAAGAATTAAAGCTGTTCGCGAACTCTCAAAAAGTCGGACAAGGCCTGCC
GCTTTGGCTGCCAAAAGGCGCAACAATCCGCCGCGTCATCGAGCGCTACATTGTCGATAAAGAAATCAGCCTCGGCTATG
AGCACGTATACACACCTGTGCTAGGCAGCAAAGAGCTGTATGAAACATCAGGACACTGGGATCATTATCAGGAAGGCATG
TTCCCTCCGATGGAAATGGACAATGAAACACTTGTATTGCGTCCGATGAACTGTCCGCACCATATGATGATTTACAAACA
AGACATTCACAGTTACCGCGAGCTTCCGATTCGTATTGCAGAGCTTGGAACGATGCACCGCTATGAAATGTCAGGTGCGC
TGTCAGGACTTCAACGGGTACGCGGAATGACGTTAAATGATGCACACATCTTTGTGCGCCCGGATCAAATTAAGGATGAG
TTTATCCGTACAGTCCGTTTAATCCAGGATGTTTATGAAGACTTCGGTTTAAGTGATTACACATTCCGTCTGTCTTACCG
CGATCCGGAAGATACAGAGAAATATTTTGATGACGATGAAATGTGGAACAAAGCGCAATCCATGCTGAAAGAGGCAATGG
ATGAAATCGGCCACGACTATTACGAAGCAGAAGGTGAAGCGGCATTCTACGGACCTAAACTTGATGTTCAGGTGAAAACT
GCGATCGGAAAAGAAGAAACACTGTCTACGGTTCAGCTTGATTTCTTATTGCCGGAACGTTTCGACCTGACATACATCGG
TGAGGACGGCAAGCAGCACCGTCCGGTTGTCATTCACAGAGGTGTCGTTTCAACAATGGAACGCTTTGTTGCTTTCTTAA
TCGAAGAACACAAAGGCGCGCTGCCGACATGGCTTGCACCGGTTCAGTTCCAAGTCATCCCGGTTTCTCCGGCTGTGCAT
TTAGACTACGCGAAAAAAGTGCAGGAACGCCTGCAATGTGAAGGCCTGCGTGTTGAAGTCGACAGCCGCGATGAAAAAAT
CGGCTACAAAATCCGTGAAGCGCAAATGCAAAAAATCCCGTACATGCTGGTGGTCGGTGACCAAGAAGCAGAAAACGGAG
CGGTAAACGTGCGTAAATACGGAGAACAGAACTCTGAAACCATTTCACTTGATGAGTTTGTGAAAAAGGCAGTAGCTGAA
GCGAAAAAATAA

Upstream 100 bases:

>100_bases
GGCTTTGCGGAAAAAAGGGTGGAACCACGATTCCGTTTATTCAACCTCGTCCCTTTCATAGGGGGCGGGGTTTTTATATG
CAAAAAAAGGAGTGACAAAG

Downstream 100 bases:

>100_bases
AATAAAAAAGCATGATCTCATTGAAGAGATCATGCTTTTTTTATTTCTCTAATATAGGAAACAAATCATGAAGGCTGCTG
ATGATGTAATCAGGCTTTAC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MSDMVKITFPDGAVKEFAKGTTTEDIAASISPGLKKKSLAGKLNGKEIDLRTPINEDGTVEIITEGSEEGLQIMRHSAAH
LLAQAIKRIYKDVKFGVGPVIENGFYYDVEMDEAITPEDLPKIEKEMKKIVNANLPIVRKEVSREEAKARFAEIGDDLKL
ELLDAIPEGETVSIYEQGEFFDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSKNQMLQRVYGTAFFKKADLEEHLRMLEE
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKEISLGYEHVYTPVLGSKELYETSGHWDHYQEGM
FPPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDE
FIRTVRLIQDVYEDFGLSDYTFRLSYRDPEDTEKYFDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKT
AIGKEETLSTVQLDFLLPERFDLTYIGEDGKQHRPVVIHRGVVSTMERFVAFLIEEHKGALPTWLAPVQFQVIPVSPAVH
LDYAKKVQERLQCEGLRVEVDSRDEKIGYKIREAQMQKIPYMLVVGDQEAENGAVNVRKYGEQNSETISLDEFVKKAVAE
AKK

Sequences:

>Translated_643_residues
MSDMVKITFPDGAVKEFAKGTTTEDIAASISPGLKKKSLAGKLNGKEIDLRTPINEDGTVEIITEGSEEGLQIMRHSAAH
LLAQAIKRIYKDVKFGVGPVIENGFYYDVEMDEAITPEDLPKIEKEMKKIVNANLPIVRKEVSREEAKARFAEIGDDLKL
ELLDAIPEGETVSIYEQGEFFDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSKNQMLQRVYGTAFFKKADLEEHLRMLEE
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKEISLGYEHVYTPVLGSKELYETSGHWDHYQEGM
FPPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDE
FIRTVRLIQDVYEDFGLSDYTFRLSYRDPEDTEKYFDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKT
AIGKEETLSTVQLDFLLPERFDLTYIGEDGKQHRPVVIHRGVVSTMERFVAFLIEEHKGALPTWLAPVQFQVIPVSPAVH
LDYAKKVQERLQCEGLRVEVDSRDEKIGYKIREAQMQKIPYMLVVGDQEAENGAVNVRKYGEQNSETISLDEFVKKAVAE
AKK
>Mature_642_residues
SDMVKITFPDGAVKEFAKGTTTEDIAASISPGLKKKSLAGKLNGKEIDLRTPINEDGTVEIITEGSEEGLQIMRHSAAHL
LAQAIKRIYKDVKFGVGPVIENGFYYDVEMDEAITPEDLPKIEKEMKKIVNANLPIVRKEVSREEAKARFAEIGDDLKLE
LLDAIPEGETVSIYEQGEFFDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSKNQMLQRVYGTAFFKKADLEEHLRMLEEA
KERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKEISLGYEHVYTPVLGSKELYETSGHWDHYQEGMF
PPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDEF
IRTVRLIQDVYEDFGLSDYTFRLSYRDPEDTEKYFDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKTA
IGKEETLSTVQLDFLLPERFDLTYIGEDGKQHRPVVIHRGVVSTMERFVAFLIEEHKGALPTWLAPVQFQVIPVSPAVHL
DYAKKVQERLQCEGLRVEVDSRDEKIGYKIREAQMQKIPYMLVVGDQEAENGAVNVRKYGEQNSETISLDEFVKKAVAEA
KK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=653, Percent_Identity=38.2848392036753, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI38570111, Length=621, Percent_Identity=38.0032206119163, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI20070344, Length=654, Percent_Identity=35.0152905198777, Blast_Score=378, Evalue=1e-105,
Organism=Escherichia coli, GI1788013, Length=640, Percent_Identity=42.03125, Blast_Score=543, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71984192, Length=639, Percent_Identity=37.245696400626, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71984184, Length=639, Percent_Identity=37.245696400626, Blast_Score=435, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322112, Length=656, Percent_Identity=36.5853658536585, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322654, Length=406, Percent_Identity=34.9753694581281, Blast_Score=264, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24583839, Length=635, Percent_Identity=37.1653543307087, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24583842, Length=635, Percent_Identity=37.1653543307087, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI28574141, Length=635, Percent_Identity=37.1653543307087, Blast_Score=436, Evalue=1e-122,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT1_BACSU (P18255)

Other databases:

- EMBL:   M36594
- EMBL:   Z75208
- EMBL:   AF008220
- EMBL:   AL009126
- EMBL:   X04963
- PIR:   B37770
- RefSeq:   NP_390773.1
- ProteinModelPortal:   P18255
- SMR:   P18255
- EnsemblBacteria:   EBBACT00000003316
- GeneID:   937410
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28950
- NMPDR:   fig|224308.1.peg.2898
- GenoList:   BSU28950
- GeneTree:   EBGT00050000001167
- HOGENOM:   HBG352811
- OMA:   NDETMST
- PhylomeDB:   P18255
- ProtClustDB:   PRK00413
- BioCyc:   BSUB:BSU28950-MONOMER
- BRENDA:   6.1.1.3
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 73516; Mature: 73385

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMVKITFPDGAVKEFAKGTTTEDIAASISPGLKKKSLAGKLNGKEIDLRTPINEDGTV
CCCCEEEECCCHHHHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCCCE
EIITEGSEEGLQIMRHSAAHLLAQAIKRIYKDVKFGVGPVIENGFYYDVEMDEAITPEDL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCHHH
PKIEKEMKKIVNANLPIVRKEVSREEAKARFAEIGDDLKLELLDAIPEGETVSIYEQGEF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEECCCH
FDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSKNQMLQRVYGTAFFKKADLEEHLRMLEE
HHHHCCCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKEISLGYEHVYTPV
HHHHHHHHHHHHHHHHCCCHHHCCCCCEECCCCHHHHHHHHHHHCCCHHHCCHHHHHCCC
LGSKELYETSGHWDHYQEGMFPPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIA
CCCHHHHHCCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCHHHEEHHHHHHHHHCCEEEE
ELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDEFIRTVRLIQDVYEDFGLSDY
HHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCE
TFRLSYRDPEDTEKYFDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKT
EEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEEEE
AIGKEETLSTVQLDFLLPERFDLTYIGEDGKQHRPVVIHRGVVSTMERFVAFLIEEHKGA
CCCCHHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCC
LPTWLAPVQFQVIPVSPAVHLDYAKKVQERLQCEGLRVEVDSRDEKIGYKIREAQMQKIP
CCCEECCEEEEEEECCCCEEHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCC
YMLVVGDQEAENGAVNVRKYGEQNSETISLDEFVKKAVAEAKK
EEEEECCCCCCCCCEEEHCCCCCCCCEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDMVKITFPDGAVKEFAKGTTTEDIAASISPGLKKKSLAGKLNGKEIDLRTPINEDGTV
CCCEEEECCCHHHHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCCCE
EIITEGSEEGLQIMRHSAAHLLAQAIKRIYKDVKFGVGPVIENGFYYDVEMDEAITPEDL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCHHH
PKIEKEMKKIVNANLPIVRKEVSREEAKARFAEIGDDLKLELLDAIPEGETVSIYEQGEF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEECCCH
FDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSKNQMLQRVYGTAFFKKADLEEHLRMLEE
HHHHCCCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKEISLGYEHVYTPV
HHHHHHHHHHHHHHHHCCCHHHCCCCCEECCCCHHHHHHHHHHHCCCHHHCCHHHHHCCC
LGSKELYETSGHWDHYQEGMFPPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIA
CCCHHHHHCCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCHHHEEHHHHHHHHHCCEEEE
ELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDEFIRTVRLIQDVYEDFGLSDY
HHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCE
TFRLSYRDPEDTEKYFDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKT
EEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEEEE
AIGKEETLSTVQLDFLLPERFDLTYIGEDGKQHRPVVIHRGVVSTMERFVAFLIEEHKGA
CCCCHHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCC
LPTWLAPVQFQVIPVSPAVHLDYAKKVQERLQCEGLRVEVDSRDEKIGYKIREAQMQKIP
CCCEECCEEEEEEECCCCEEHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCC
YMLVVGDQEAENGAVNVRKYGEQNSETISLDEFVKKAVAEAKK
EEEEECCCCCCCCCEEEHCCCCCCCCEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2115870; 8969504; 9387221; 9384377; 3027671; 1379177