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The map label for this gene is yfnC

Identifier: 16077799

GI number: 16077799

Start: 803317

End: 804546

Strand: Reverse

Name: yfnC

Synonym: BSU07320

Alternate gene names: 16077799

Gene position: 804546-803317 (Counterclockwise)

Preceding gene: 16077800

Following gene: 16077791

Centisome position: 19.08

GC content: 52.76

Gene sequence:

>1230_bases
ATGGCTATCGCCGCCCCGTTAAAAGAAAAAACAGTTCAAAAACCAGGCACCACGGTGTATCCGATTTTGATTATTATCGG
AATTTGCCATATGCTCAACGATTCGCTTCAGGCTGTGATTCCGGCCATGTTCCCTATTTTGGAACGCTCCATGAGCCTGA
CGTTTACACAGCTCGGCATCATTGCTTTCACATTAAACATGGTGTCATCCGTCATGCAGCCTGTTGTCGGCTGGTATACC
GATAAACGGCCGCGGCCGTATGCGCTGCCAGTCGGTTTGACCGCCAGCATGCTTGGCATTTTGGGCCTTGCATTTGCCCC
GTCATTCATAACGATCTTATGCTGTGTGTTTTTCATTGGATTAGGCTCGGCCATTTTCCATCCGGAGGGCTCCCGTGTGG
CGTATATGGCCGCCGGCACAAAACGCGGGCTCGCCCAATCGATTTATCAGGTGGGCGGAAACTCAGGCCAGGCGATGGCA
CCGCTGATCACCGCGCTGATCCTTGTGCCGCTCGGGCAATTCGGCGCTGTTTGGTTCACGCTTGTCGCCGCGCTTGCTGT
TATGTTTCTCATGTATATTGCGAAATGGTACGCCTCACGTCTCGGCAGCCTTGCCCAGAAATCAGGCAAGCAAAAGAAAA
CGGCTGAAAACACGGCCATCACCAAATCTGTTGTATCTGCTCTTATTATTATTATATTTCTCATTTTTGCTCGTTCTTGG
TATACGAGTGCGATCGGTAACTTTTACACGTTTTACGCGATGGATACCTATCACGTAAGCATTCAGCAGGCACAAAGCTA
TATTTTTGTCTTCTTGCTGTTCGGCGCGATCGGCACGTTTCTCGGCGGCCCGCTTGCAGACCGTTTTGGAAAACGCTTCG
TTATCCTCGGTTCGCTGTTATGCTCGGCGCCGCTGGCGATTGTGCTGCCATTTGCCGGCCCGGTTCTCGCCTACGGCGTG
CTCGCATTGATTGGCTTGGTTCTGATGTCGAGCTTTTCTGTAACGGTCGTCTACGCACAGGAGCTAGTTCCTGGAAAAAT
CGGCACAATGTCCGGTTTAACCGTCGGGCTTGCCTTCGGGATGGGCGCAATCGGCGCTGTGGCGCTGGGCGCTCTGATTG
ATGCCGCGGGACTGACCCCGACGATGATCGCCATTGCCTTTCTCCCTGTCCTCGGCATCCTTGCCTTCCTATTGCCAAGT
GACCAGAAGCTGAGAGAATGGCATAGCTGA

Upstream 100 bases:

>100_bases
GGTATTTCTTTCATAAGAATCTTTTTTCCTCTTGATAAATTCTTCACCTTATGAATAATATGGTGGTATGCTTTGGTTAT
ATATGTAAGGAGGAGACTCA

Downstream 100 bases:

>100_bases
AAAAACCCCTGCCAGGCGGCAGGGGGTTTTTTAATCCAGCTGGATAAAATGAGTGTCAGGCACTGCGTGCTTCGGGATAA
AGCCATCCGCAAAGTCCGGA

Product: efflux transporter

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MAIAAPLKEKTVQKPGTTVYPILIIIGICHMLNDSLQAVIPAMFPILERSMSLTFTQLGIIAFTLNMVSSVMQPVVGWYT
DKRPRPYALPVGLTASMLGILGLAFAPSFITILCCVFFIGLGSAIFHPEGSRVAYMAAGTKRGLAQSIYQVGGNSGQAMA
PLITALILVPLGQFGAVWFTLVAALAVMFLMYIAKWYASRLGSLAQKSGKQKKTAENTAITKSVVSALIIIIFLIFARSW
YTSAIGNFYTFYAMDTYHVSIQQAQSYIFVFLLFGAIGTFLGGPLADRFGKRFVILGSLLCSAPLAIVLPFAGPVLAYGV
LALIGLVLMSSFSVTVVYAQELVPGKIGTMSGLTVGLAFGMGAIGAVALGALIDAAGLTPTMIAIAFLPVLGILAFLLPS
DQKLREWHS

Sequences:

>Translated_409_residues
MAIAAPLKEKTVQKPGTTVYPILIIIGICHMLNDSLQAVIPAMFPILERSMSLTFTQLGIIAFTLNMVSSVMQPVVGWYT
DKRPRPYALPVGLTASMLGILGLAFAPSFITILCCVFFIGLGSAIFHPEGSRVAYMAAGTKRGLAQSIYQVGGNSGQAMA
PLITALILVPLGQFGAVWFTLVAALAVMFLMYIAKWYASRLGSLAQKSGKQKKTAENTAITKSVVSALIIIIFLIFARSW
YTSAIGNFYTFYAMDTYHVSIQQAQSYIFVFLLFGAIGTFLGGPLADRFGKRFVILGSLLCSAPLAIVLPFAGPVLAYGV
LALIGLVLMSSFSVTVVYAQELVPGKIGTMSGLTVGLAFGMGAIGAVALGALIDAAGLTPTMIAIAFLPVLGILAFLLPS
DQKLREWHS
>Mature_408_residues
AIAAPLKEKTVQKPGTTVYPILIIIGICHMLNDSLQAVIPAMFPILERSMSLTFTQLGIIAFTLNMVSSVMQPVVGWYTD
KRPRPYALPVGLTASMLGILGLAFAPSFITILCCVFFIGLGSAIFHPEGSRVAYMAAGTKRGLAQSIYQVGGNSGQAMAP
LITALILVPLGQFGAVWFTLVAALAVMFLMYIAKWYASRLGSLAQKSGKQKKTAENTAITKSVVSALIIIIFLIFARSWY
TSAIGNFYTFYAMDTYHVSIQQAQSYIFVFLLFGAIGTFLGGPLADRFGKRFVILGSLLCSAPLAIVLPFAGPVLAYGVL
ALIGLVLMSSFSVTVVYAQELVPGKIGTMSGLTVGLAFGMGAIGAVALGALIDAAGLTPTMIAIAFLPVLGILAFLLPSD
QKLREWHS

Specific function: Confers The Resistance Against Fosmidomycin. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily

Homologues:

Organism=Escherichia coli, GI1786686, Length=386, Percent_Identity=43.7823834196891, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFNC_BACSU (O06481)

Other databases:

- EMBL:   D86418
- EMBL:   AL009126
- PIR:   F69814
- RefSeq:   NP_388613.1
- ProteinModelPortal:   O06481
- EnsemblBacteria:   EBBACT00000003568
- GeneID:   936104
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07320
- NMPDR:   fig|224308.1.peg.732
- GenoList:   BSU07320
- GeneTree:   EBGT00070000032171
- HOGENOM:   HBG611985
- OMA:   NLFWTGV
- PhylomeDB:   O06481
- ProtClustDB:   CLSK2518207
- BioCyc:   BSUB:BSU07320-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 43489; Mature: 43358

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd4db3f8)-; HASH(0xca63060)-; HASH(0xd652d24)-; HASH(0xda5f1ec)-; HASH(0xda6e678)-; HASH(0xd3bb608)-; HASH(0xccc286c)-; HASH(0xc8de40c)-; HASH(0xd526ea4)-; HASH(0xd32b378)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCH
>Mature Secondary Structure 
AIAAPLKEKTVQKPGTTVYPILIIIGICHMLNDSLQAVIPAMFPILERSMSLTFTQLGI
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFTLNMVSSVMQPVVGWYTDKRPRPYALPVGLTASMLGILGLAFAPSFITILCCVFFIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGSAIFHPEGSRVAYMAAGTKRGLAQSIYQVGGNSGQAMAPLITALILVPLGQFGAVWFT
CCCHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
LVAALAVMFLMYIAKWYASRLGSLAQKSGKQKKTAENTAITKSVVSALIIIIFLIFARSW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTSAIGNFYTFYAMDTYHVSIQQAQSYIFVFLLFGAIGTFLGGPLADRFGKRFVILGSLL
HHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHH
CSAPLAIVLPFAGPVLAYGVLALIGLVLMSSFSVTVVYAQELVPGKIGTMSGLTVGLAFG
HHCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEEHHHHCCCCCCCHHHHHHHHHHH
MGAIGAVALGALIDAAGLTPTMIAIAFLPVLGILAFLLPSDQKLREWHS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377