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The map label for this gene is opuE

Identifier: 16077734

GI number: 16077734

Start: 726840

End: 728318

Strand: Reverse

Name: opuE

Synonym: BSU06660

Alternate gene names: 16077734

Gene position: 728318-726840 (Counterclockwise)

Preceding gene: 16077738

Following gene: 16077733

Centisome position: 17.28

GC content: 46.11

Gene sequence:

>1479_bases
GTGAGTATTGAAATTATTATATCTTTAGGAATTTACTTTATTGCCATGCTGTTAATCGGCTGGTATGCATTCAAGAAAAC
CACTGACATCAATGATTACATGCTTGGAGGAAGAGGCCTCGGACCATTCGTTACTGCTTTGTCTGCCGGCGCGGCCGATA
TGAGCGGATGGATGCTGATGGGTGTTCCCGGCGCGATGTTTGCGACAGGGCTGTCCACCTTATGGCTTGCTTTAGGTCTT
ACAATCGGGGCGTACTCAAATTACTTGCTGCTGGCTCCAAGGCTGCGTGCTTATACAGAAGCGGCAGATGATGCGATTAC
AATTCCTGATTTCTTTGATAAACGATTCCAGCATTCTTCATCACTGCTGAAAATCGTATCTGCATTGATCATCATGATCT
TTTTCACATTGTATACCTCTTCCGGTATGGTATCTGGCGGACGGCTGTTTGAATCTGCTTTTGGTGCGGATTACAAATTA
GGCCTGTTTCTGACCACCGCAGTTGTCGTGTTATACACACTGTTTGGCGGTTTCCTTGCTGTCAGTCTGACTGACTTCGT
GCAGGGTGCGATCATGTTTGCAGCACTAGTGCTTGTGCCGATCGTTGCTTTTACCCATGTGGGCGGCGTTGCTCCGACTT
TCCATGAAATCGACGCTGTTAATCCACATTTATTGGATATTTTTAAAGGCGCGAGCGTCATTAGCATTATTTCCTATTTA
GCCTGGGGGTTGGGCTACTACGGCCAGCCTCATATCATCGTCCGATTTATGGCCATTAAAGACATTAAAGATTTAAAACC
TGCACGCCGAATCGGCATGAGCTGGATGATCATTACAGTTCTAGGTTCCGTGCTGACGGGTTTAATCGGTGTTGCATACG
CCCATAAATTCGGAGTTGCTGTAAAAGATCCGGAGATGATTTTCATTATCTTCTCTAAAATCTTGTTCCACCCATTAATT
ACAGGGTTCTTATTGTCAGCTATTTTGGCTGCCATCATGAGTTCGATTTCCTCACAGCTCTTGGTCACAGCAAGTGCTGT
AACAGAGGATTTATACCGGTCATTTTTCAGACGAAAAGCTTCTGATAAAGAATTGGTCATGATCGGGCGATTGTCCGTCT
TAGTCATTGCCGTTATCGCGGTACTCTTGTCATTGAACCCGAACAGCACCATTCTTGATTTGGTCGGATACGCTTGGGCC
GGCTTCGGTTCAGCTTTCGGGCCTGCGATTCTGCTCAGTCTGTATTGGAAACGAATGAACGAATGGGGCGCGCTTGCTGC
CATGATTGTCGGGGCTGCGACCGTGTTGATCTGGATTACAACCGGCCTGGCAAAATCAACCGGTGTGTACGAAATCATCC
CTGGGTTTATTCTCAGTATGATTGCCGGCATCATTGTCAGCATGATCACAAAACGTCCTGCAAAAGCCTCTTACCGATTA
TTCGGTGTCATGGAAAAGCTGTTAAAACGCAAAAAGTAA

Upstream 100 bases:

>100_bases
AAAGAATTTCTTTTGTTTTGCACTTACGCCAGGCTGCTTAACTGGCACCATCAATTTGAGAGGTGATGCAACATTATTAG
AAATGAAGAGAGGGTAAAAC

Downstream 100 bases:

>100_bases
GCAATAAGCCATCAAGCGCAGCTTGGTGGCTTTTGTGCTCTTGTTTTGCTATAAATACTGTGAGAAAATCATATTTATTT
AAAAACACCATAGAGAAGGG

Product: proline transporter

Products: NA

Alternate protein names: Sodium/proline symporter

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSIEIIISLGIYFIAMLLIGWYAFKKTTDINDYMLGGRGLGPFVTALSAGAADMSGWMLMGVPGAMFATGLSTLWLALGL
TIGAYSNYLLLAPRLRAYTEAADDAITIPDFFDKRFQHSSSLLKIVSALIIMIFFTLYTSSGMVSGGRLFESAFGADYKL
GLFLTTAVVVLYTLFGGFLAVSLTDFVQGAIMFAALVLVPIVAFTHVGGVAPTFHEIDAVNPHLLDIFKGASVISIISYL
AWGLGYYGQPHIIVRFMAIKDIKDLKPARRIGMSWMIITVLGSVLTGLIGVAYAHKFGVAVKDPEMIFIIFSKILFHPLI
TGFLLSAILAAIMSSISSQLLVTASAVTEDLYRSFFRRKASDKELVMIGRLSVLVIAVIAVLLSLNPNSTILDLVGYAWA
GFGSAFGPAILLSLYWKRMNEWGALAAMIVGAATVLIWITTGLAKSTGVYEIIPGFILSMIAGIIVSMITKRPAKASYRL
FGVMEKLLKRKK

Sequences:

>Translated_492_residues
MSIEIIISLGIYFIAMLLIGWYAFKKTTDINDYMLGGRGLGPFVTALSAGAADMSGWMLMGVPGAMFATGLSTLWLALGL
TIGAYSNYLLLAPRLRAYTEAADDAITIPDFFDKRFQHSSSLLKIVSALIIMIFFTLYTSSGMVSGGRLFESAFGADYKL
GLFLTTAVVVLYTLFGGFLAVSLTDFVQGAIMFAALVLVPIVAFTHVGGVAPTFHEIDAVNPHLLDIFKGASVISIISYL
AWGLGYYGQPHIIVRFMAIKDIKDLKPARRIGMSWMIITVLGSVLTGLIGVAYAHKFGVAVKDPEMIFIIFSKILFHPLI
TGFLLSAILAAIMSSISSQLLVTASAVTEDLYRSFFRRKASDKELVMIGRLSVLVIAVIAVLLSLNPNSTILDLVGYAWA
GFGSAFGPAILLSLYWKRMNEWGALAAMIVGAATVLIWITTGLAKSTGVYEIIPGFILSMIAGIIVSMITKRPAKASYRL
FGVMEKLLKRKK
>Mature_491_residues
SIEIIISLGIYFIAMLLIGWYAFKKTTDINDYMLGGRGLGPFVTALSAGAADMSGWMLMGVPGAMFATGLSTLWLALGLT
IGAYSNYLLLAPRLRAYTEAADDAITIPDFFDKRFQHSSSLLKIVSALIIMIFFTLYTSSGMVSGGRLFESAFGADYKLG
LFLTTAVVVLYTLFGGFLAVSLTDFVQGAIMFAALVLVPIVAFTHVGGVAPTFHEIDAVNPHLLDIFKGASVISIISYLA
WGLGYYGQPHIIVRFMAIKDIKDLKPARRIGMSWMIITVLGSVLTGLIGVAYAHKFGVAVKDPEMIFIIFSKILFHPLIT
GFLLSAILAAIMSSISSQLLVTASAVTEDLYRSFFRRKASDKELVMIGRLSVLVIAVIAVLLSLNPNSTILDLVGYAWAG
FGSAFGPAILLSLYWKRMNEWGALAAMIVGAATVLIWITTGLAKSTGVYEIIPGFILSMIAGIIVSMITKRPAKASYRLF
GVMEKLLKRKK

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family

Homologues:

Organism=Homo sapiens, GI310128183, Length=478, Percent_Identity=56.2761506276151, Blast_Score=539, Evalue=1e-153,
Organism=Homo sapiens, GI14140236, Length=556, Percent_Identity=24.1007194244604, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI4507031, Length=538, Percent_Identity=24.1635687732342, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI109659836, Length=385, Percent_Identity=24.1558441558442, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI109659839, Length=401, Percent_Identity=23.4413965087282, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI11141885, Length=445, Percent_Identity=23.3707865168539, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1787251, Length=485, Percent_Identity=49.4845360824742, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI87082237, Length=476, Percent_Identity=29.8319327731092, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1790503, Length=453, Percent_Identity=27.5938189845475, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17539284, Length=457, Percent_Identity=23.4135667396061, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115533094, Length=382, Percent_Identity=24.0837696335079, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648033, Length=457, Percent_Identity=23.6323851203501, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356865, Length=457, Percent_Identity=23.6323851203501, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24651739, Length=478, Percent_Identity=21.5481171548117, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OPUE_BACSU (O06493)

Other databases:

- EMBL:   U92466
- EMBL:   AF011545
- EMBL:   AL009126
- PIR:   H69670
- RefSeq:   NP_388548.1
- ProteinModelPortal:   O06493
- EnsemblBacteria:   EBBACT00000003013
- GeneID:   939439
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU06660
- NMPDR:   fig|224308.1.peg.667
- GenoList:   BSU06660
- GeneTree:   EBGT00070000031818
- HOGENOM:   HBG499520
- OMA:   FRRNASD
- PhylomeDB:   O06493
- ProtClustDB:   CLSK886821
- BioCyc:   BSUB:BSU06660-MONOMER
- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900
- PANTHER:   PTHR11819
- TIGRFAMs:   TIGR02121
- TIGRFAMs:   TIGR00813

Pfam domain/function: PF00474 SSF

EC number: NA

Molecular weight: Translated: 53283; Mature: 53152

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00456 NA_SOLUT_SYMP_1; PS00457 NA_SOLUT_SYMP_2; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xbacafd0)-; HASH(0x94663e0)-; HASH(0xd424880)-; HASH(0xd5f7890)-; HASH(0xd30cec0)-; HASH(0xcafb740)-; HASH(0xd1e8788)-; HASH(0xd4061f0)-; HASH(0xd4a1af0)-; HASH(0xcd03ca4)-; HASH(0xd420288)-; HASH(0xd38af7c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIIISLGIYFIAMLLIGWYAFKKTTDINDYMLGGRGLGPFVTALSAGAADMSGWMLM
CCEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHCCCCHHCCEEEE
GVPGAMFATGLSTLWLALGLTIGAYSNYLLLAPRLRAYTEAADDAITIPDFFDKRFQHSS
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCEECCHHHHHHHHHHH
SLLKIVSALIIMIFFTLYTSSGMVSGGRLFESAFGADYKLGLFLTTAVVVLYTLFGGFLA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VSLTDFVQGAIMFAALVLVPIVAFTHVGGVAPTFHEIDAVNPHLLDIFKGASVISIISYL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHCCCHHHHHHHHH
AWGLGYYGQPHIIVRFMAIKDIKDLKPARRIGMSWMIITVLGSVLTGLIGVAYAHKFGVA
HHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCE
VKDPEMIFIIFSKILFHPLITGFLLSAILAAIMSSISSQLLVTASAVTEDLYRSFFRRKA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SDKELVMIGRLSVLVIAVIAVLLSLNPNSTILDLVGYAWAGFGSAFGPAILLSLYWKRMN
CCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EWGALAAMIVGAATVLIWITTGLAKSTGVYEIIPGFILSMIAGIIVSMITKRPAKASYRL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FGVMEKLLKRKK
HHHHHHHHHCCH
>Mature Secondary Structure 
SIEIIISLGIYFIAMLLIGWYAFKKTTDINDYMLGGRGLGPFVTALSAGAADMSGWMLM
CEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHCCCCHHCCEEEE
GVPGAMFATGLSTLWLALGLTIGAYSNYLLLAPRLRAYTEAADDAITIPDFFDKRFQHSS
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCEECCHHHHHHHHHHH
SLLKIVSALIIMIFFTLYTSSGMVSGGRLFESAFGADYKLGLFLTTAVVVLYTLFGGFLA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VSLTDFVQGAIMFAALVLVPIVAFTHVGGVAPTFHEIDAVNPHLLDIFKGASVISIISYL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHCCCHHHHHHHHH
AWGLGYYGQPHIIVRFMAIKDIKDLKPARRIGMSWMIITVLGSVLTGLIGVAYAHKFGVA
HHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCE
VKDPEMIFIIFSKILFHPLITGFLLSAILAAIMSSISSQLLVTASAVTEDLYRSFFRRKA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SDKELVMIGRLSVLVIAVIAVLLSLNPNSTILDLVGYAWAGFGSAFGPAILLSLYWKRMN
CCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EWGALAAMIVGAATVLIWITTGLAKSTGVYEIIPGFILSMIAGIIVSMITKRPAKASYRL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FGVMEKLLKRKK
HHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377