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The map label for this gene is purF [H]

Identifier: 159901083

GI number: 159901083

Start: 5848775

End: 5850181

Strand: Direct

Name: purF [H]

Synonym: Haur_4571

Alternate gene names: 159901083

Gene position: 5848775-5850181 (Clockwise)

Preceding gene: 159901080

Following gene: 159901086

Centisome position: 92.16

GC content: 52.17

Gene sequence:

>1407_bases
ATGTTCAGTGATTCGCCCCACGAGGAATGCGGCGTTTTTGGGATTTACGCTCCCAGCGAAGATGTAGCACGTATCACATT
TTTCAGTTTATACGCTTTGCAGCACCGTGGTCAGGAAAGCGCAGGGATTGCGGTTTCCGATGGCAAGACCATCAATACCC
ACAAAGAAATGGGCTTGGTATCGCAGATTTTCGATGAGCGTTCGCTGCGCCACCTCAAAGGTCACATTGCGATTGGCCAT
ACCCGCTACTCAACCACTGGTTCATCACAAGTAATCAATGCCCAACCGTTTACAATGCACACGTTGCTTGGCCCACTGGC
AGTTGGCCACAACGGCAACCTGACCAATGCTGCCTCGCTGCGCCGTCAATTGATGGAACGTGGTGTCGGGTTAATGACAT
CTTCTGATAGTGAAGTGGCAACGATGCTGCTCGCTGGCCTTGAGGGTAATACTTGGTCACAACGCATCGATGCCTTTACC
AACTGTGTCGAAGGTGCCTATTGTCTGACAGTCTTGACCCGCGATGCGCTCTACGCCGTGCGCGACCCATGGGGTTTGCG
GCCATTATGCCTTGGGCGTTTTGGCGATGCTGGCTGGGTCGTGGCCTCGGAATCATGCGCCTTCGATACAATTGGCGCTG
AATTTATCCGAGAGATCGATCCTGGCGAGGTGTTGCAGATCGATCGTGATGGCCCACGCACGATTGCCAAACACCCTGCT
CAGCAGCAAGCCTTTTGTTTATTTGAATATATTTACTTCTCGCGCCCCGATAGCTATCTACAAGGTCAATTAATTCATGA
AGTACGCATGCGCTTAGGCCATGAGTTGGCACGGGAAGCTCCGGCGGAGGCTGATATCGTGATCGGTGTGCCTGATTCGG
CCTTGCCAGCTGCCCTTGGCTTTTCGCAGGCCAGCGGGATTCCCTATGGCGATGGCTTGATCAAAAATCGTTATATTGGC
CGCACCTTCATTCAGCCCGATCAACGGTTGCGCCAACAAGGCGTTGCGCTCAAGCTCAATCCATTGCCAAATGTGCTCAA
TGGCAAGCGGGTAATTTTGATTGACGATACGATTGTGCGCGGCACAACCAGCGGGCCAATTATCAAACTTTTGCGCAAAG
CTGGAGCCACCGAAGTCCATATGCGCGTATCTGCGCCGCCAATTCGCCATCCATGTTTTATGGGTGTGGATATGGCTACT
CAGCCCGAACTGATTGCCTTCAACAAAACGGAGGCTGAAATTTGCGAAACGATTGGAGCCGATTCGTTAGCCTACCTTTC
GATGGATGGCTTGATTCGCGCTACGCGGCGAGATGCGAATGGCTTTTGTGGAGCCTGTTTCAGCGGCAAGTATCCCTTCC
CAATTCACGAAGCAGGCAAAGAAATTTTCGAGCTAACCAGCATCTAA

Upstream 100 bases:

>100_bases
AAAGCATGCGGGGGATATTTCTGAACAGTTGTATAATGATTACACGATCCGTCGCACGACGCGGCGACAACCGTAGCTTG
GAATCAAGGAAGGGCTAACC

Downstream 100 bases:

>100_bases
AGCATTAAATAGCAACCGCCAGTCATTAAACTTGCCTGGCGGTTGTTATTTTAGGCATCGATTGTGCCGACTGCGACTGG
ATTTGAGGTTGGCTGTTGGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MFSDSPHEECGVFGIYAPSEDVARITFFSLYALQHRGQESAGIAVSDGKTINTHKEMGLVSQIFDERSLRHLKGHIAIGH
TRYSTTGSSQVINAQPFTMHTLLGPLAVGHNGNLTNAASLRRQLMERGVGLMTSSDSEVATMLLAGLEGNTWSQRIDAFT
NCVEGAYCLTVLTRDALYAVRDPWGLRPLCLGRFGDAGWVVASESCAFDTIGAEFIREIDPGEVLQIDRDGPRTIAKHPA
QQQAFCLFEYIYFSRPDSYLQGQLIHEVRMRLGHELAREAPAEADIVIGVPDSALPAALGFSQASGIPYGDGLIKNRYIG
RTFIQPDQRLRQQGVALKLNPLPNVLNGKRVILIDDTIVRGTTSGPIIKLLRKAGATEVHMRVSAPPIRHPCFMGVDMAT
QPELIAFNKTEAEICETIGADSLAYLSMDGLIRATRRDANGFCGACFSGKYPFPIHEAGKEIFELTSI

Sequences:

>Translated_468_residues
MFSDSPHEECGVFGIYAPSEDVARITFFSLYALQHRGQESAGIAVSDGKTINTHKEMGLVSQIFDERSLRHLKGHIAIGH
TRYSTTGSSQVINAQPFTMHTLLGPLAVGHNGNLTNAASLRRQLMERGVGLMTSSDSEVATMLLAGLEGNTWSQRIDAFT
NCVEGAYCLTVLTRDALYAVRDPWGLRPLCLGRFGDAGWVVASESCAFDTIGAEFIREIDPGEVLQIDRDGPRTIAKHPA
QQQAFCLFEYIYFSRPDSYLQGQLIHEVRMRLGHELAREAPAEADIVIGVPDSALPAALGFSQASGIPYGDGLIKNRYIG
RTFIQPDQRLRQQGVALKLNPLPNVLNGKRVILIDDTIVRGTTSGPIIKLLRKAGATEVHMRVSAPPIRHPCFMGVDMAT
QPELIAFNKTEAEICETIGADSLAYLSMDGLIRATRRDANGFCGACFSGKYPFPIHEAGKEIFELTSI
>Mature_468_residues
MFSDSPHEECGVFGIYAPSEDVARITFFSLYALQHRGQESAGIAVSDGKTINTHKEMGLVSQIFDERSLRHLKGHIAIGH
TRYSTTGSSQVINAQPFTMHTLLGPLAVGHNGNLTNAASLRRQLMERGVGLMTSSDSEVATMLLAGLEGNTWSQRIDAFT
NCVEGAYCLTVLTRDALYAVRDPWGLRPLCLGRFGDAGWVVASESCAFDTIGAEFIREIDPGEVLQIDRDGPRTIAKHPA
QQQAFCLFEYIYFSRPDSYLQGQLIHEVRMRLGHELAREAPAEADIVIGVPDSALPAALGFSQASGIPYGDGLIKNRYIG
RTFIQPDQRLRQQGVALKLNPLPNVLNGKRVILIDDTIVRGTTSGPIIKLLRKAGATEVHMRVSAPPIRHPCFMGVDMAT
QPELIAFNKTEAEICETIGADSLAYLSMDGLIRATRRDANGFCGACFSGKYPFPIHEAGKEIFELTSI

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=46.1538461538462, Blast_Score=445, Evalue=1e-125,
Organism=Escherichia coli, GI1788651, Length=469, Percent_Identity=39.6588486140725, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1790167, Length=265, Percent_Identity=28.6792452830189, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=41.2133891213389, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323958, Length=474, Percent_Identity=36.7088607594937, Blast_Score=276, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24659598, Length=482, Percent_Identity=48.3402489626556, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI28573187, Length=485, Percent_Identity=48.659793814433, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24659604, Length=458, Percent_Identity=48.9082969432314, Blast_Score=456, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51152; Mature: 51152

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSDSPHEECGVFGIYAPSEDVARITFFSLYALQHRGQESAGIAVSDGKTINTHKEMGLV
CCCCCCCHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCEECCHHHHHHH
SQIFDERSLRHLKGHIAIGHTRYSTTGSSQVINAQPFTMHTLLGPLAVGHNGNLTNAASL
HHHHHHHHHHHHHCEEEEEEEEECCCCCCCEECCCCCCHHHHHHHHEECCCCCCCCHHHH
RRQLMERGVGLMTSSDSEVATMLLAGLEGNTWSQRIDAFTNCVEGAYCLTVLTRDALYAV
HHHHHHCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEE
RDPWGLRPLCLGRFGDAGWVVASESCAFDTIGAEFIREIDPGEVLQIDRDGPRTIAKHPA
CCCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCCC
QQQAFCLFEYIYFSRPDSYLQGQLIHEVRMRLGHELAREAPAEADIVIGVPDSALPAALG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHC
FSQASGIPYGDGLIKNRYIGRTFIQPDQRLRQQGVALKLNPLPNVLNGKRVILIDDTIVR
CHHCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEC
GTTSGPIIKLLRKAGATEVHMRVSAPPIRHPCFMGVDMATQPELIAFNKTEAEICETIGA
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCCCCCCCEEEECCCHHHHHHHHCC
DSLAYLSMDGLIRATRRDANGFCGACFSGKYPFPIHEAGKEIFELTSI
CCEEEEEHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFSDSPHEECGVFGIYAPSEDVARITFFSLYALQHRGQESAGIAVSDGKTINTHKEMGLV
CCCCCCCHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCEECCHHHHHHH
SQIFDERSLRHLKGHIAIGHTRYSTTGSSQVINAQPFTMHTLLGPLAVGHNGNLTNAASL
HHHHHHHHHHHHHCEEEEEEEEECCCCCCCEECCCCCCHHHHHHHHEECCCCCCCCHHHH
RRQLMERGVGLMTSSDSEVATMLLAGLEGNTWSQRIDAFTNCVEGAYCLTVLTRDALYAV
HHHHHHCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEE
RDPWGLRPLCLGRFGDAGWVVASESCAFDTIGAEFIREIDPGEVLQIDRDGPRTIAKHPA
CCCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCCC
QQQAFCLFEYIYFSRPDSYLQGQLIHEVRMRLGHELAREAPAEADIVIGVPDSALPAALG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHC
FSQASGIPYGDGLIKNRYIGRTFIQPDQRLRQQGVALKLNPLPNVLNGKRVILIDDTIVR
CHHCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEC
GTTSGPIIKLLRKAGATEVHMRVSAPPIRHPCFMGVDMATQPELIAFNKTEAEICETIGA
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCCCCCCCEEEECCCHHHHHHHHCC
DSLAYLSMDGLIRATRRDANGFCGACFSGKYPFPIHEAGKEIFELTSI
CCEEEEEHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]