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The map label for this gene is mutB [H]

Identifier: 159901064

GI number: 159901064

Start: 5826312

End: 5827877

Strand: Direct

Name: mutB [H]

Synonym: Haur_4552

Alternate gene names: 159901064

Gene position: 5826312-5827877 (Clockwise)

Preceding gene: 159901063

Following gene: 159901066

Centisome position: 91.8

GC content: 54.47

Gene sequence:

>1566_bases
ATGGGCAGTCAAATTACAACTGATGCTGGCATTCCAGTTGAGCCAATTTATCGTGATAACGACCGCCAGCCCGAACCTGA
TGCTGGCCAGTTTCCCTATACACGCGGCATTTACCCCACGATGTATCGAGGCCGTTTGTGGACGATGCGTCAATATGCTG
GCTTTGCTTCAGCCCACGAAACCAACGAACGCTTCCGCTATTTGCTCAACCAAGGTCAAATGGGGCTTTCCGTCGCTTTC
GATCTACCAACCCAGCTTGGCCTCGACTCGGATGATCCACGAGCTGAGGGCGAGGTTGGCAAGGTCGGGGTGGCAATCGA
TTCAATCGACGATATGGCAACCTTATTCGCGGGCATTCCGCTGGATAAAGTTAGTACCTCGATGACGATTAATGCGCCCG
CCAGTGTGCTGTTGCTGTTATACGAGTTGGTTGGCGCTCAACAAGGCGCGGCCTCGAAAACGCTCACGGGCACAATTCAA
AATGATATTCTCAAGGAATACGCTGCTCGTGGCACCTATATCTTTCCGCCACGGCCTTCGATGCGGCTGATTACCGATAC
CTTTGCTTATTGCGCCGAGCGGATTCCACGCTGGAACACGATTAGCATCAGCGGCTATCATATTCGCGAGGCTGGGGCAA
CGGCGGTGCAAGAATTGGCCTTTACCTTGGCCAACGGCATTGCCTACGTTCAGGGCGCAATTGATGCTGGCTTGCATGTC
GATGAATTTGGCCCACGCTTATCGTTCTTCTTCAATGCCCACAACAATTTCTTCGAAGAAATCGCCAAATTCCGCGCTGC
TCGCCGCATGTGGGCCACAATTATGCGTGAGCGTTTTGGTGCAACCGATGAACGCGCCTTGACCTTGCGCTTCCACACCC
AAACTGGCGGCTCGACCTTAACCTCACAGCAACCACTCAATAACGTGGTGCGGGTAACCTTGCAAGCGTTGGCGGCGGTG
ATGGGCGGCACGCAATCGCTGCACACCAACGGCTTTGATGAAGCGCTTTCGTTGCCCACCACCGAAGCTGCAACCCTCGC
CTTGCGCACCCAACAGGTCATCGCCTATGAAAGTGGCGTGACAGCTAGCGCCGATCCCTTGGGTGGTTCGTATTTGGTCG
AAAGCATGACCGACGAGATCGAGCAACGCGCCTTGGAATTAATTCGGCATATCGATGATTTGGGCGGCGCAGTACGGGCT
GTCGAAGATGGTTTCGTGCAAGCTCAAATTATGGACACCGCCTACGATTATCAAAATCGGGTTGAAAGTGGCGAGCAACT
GGTGATTGGGGTCAACAAATTTGTAACCGATGATGCCCCAGTGCCAATCTTCAAGCCTAACGAAGCGGTAGCCGCCGAAC
AATGTGCCAAATTGGCCAAACTGCGGGCCGAGCGCGATCAAGCGGCGGTTGATGCGGCTTTGGCCGATTTGCAACGCGCC
GCCGAGGGCAGTGCCAATGTGCTGTATCCAATGCGCGAAGCCTTGAGCCAACGGGCAACGGTGGGCGAGGTTTGTGGGGT
ATTGCGCCGAATTTGGGGAGAATATCGACCTGATGTGGCGATCTAA

Upstream 100 bases:

>100_bases
GACGAACGTTTGCTGCAAACGATTCGCGATGGTTTATTATCTAGTGCAGGCAGCTAAAATGGTAGCTCAGTTAGCGCTCG
CAGACACAACGAGGTGATTG

Downstream 100 bases:

>100_bases
GGGTGTTATTTTAGCAAGTGTGGTGCTGATCGTGCTGATCATCACCACACTTGCCCCAAATTTATTTGAGCAGATCGATG
GCAACGTTGGCAGTTATCTG

Product: methylmalonyl-CoA mutase large subunit

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MGSQITTDAGIPVEPIYRDNDRQPEPDAGQFPYTRGIYPTMYRGRLWTMRQYAGFASAHETNERFRYLLNQGQMGLSVAF
DLPTQLGLDSDDPRAEGEVGKVGVAIDSIDDMATLFAGIPLDKVSTSMTINAPASVLLLLYELVGAQQGAASKTLTGTIQ
NDILKEYAARGTYIFPPRPSMRLITDTFAYCAERIPRWNTISISGYHIREAGATAVQELAFTLANGIAYVQGAIDAGLHV
DEFGPRLSFFFNAHNNFFEEIAKFRAARRMWATIMRERFGATDERALTLRFHTQTGGSTLTSQQPLNNVVRVTLQALAAV
MGGTQSLHTNGFDEALSLPTTEAATLALRTQQVIAYESGVTASADPLGGSYLVESMTDEIEQRALELIRHIDDLGGAVRA
VEDGFVQAQIMDTAYDYQNRVESGEQLVIGVNKFVTDDAPVPIFKPNEAVAAEQCAKLAKLRAERDQAAVDAALADLQRA
AEGSANVLYPMREALSQRATVGEVCGVLRRIWGEYRPDVAI

Sequences:

>Translated_521_residues
MGSQITTDAGIPVEPIYRDNDRQPEPDAGQFPYTRGIYPTMYRGRLWTMRQYAGFASAHETNERFRYLLNQGQMGLSVAF
DLPTQLGLDSDDPRAEGEVGKVGVAIDSIDDMATLFAGIPLDKVSTSMTINAPASVLLLLYELVGAQQGAASKTLTGTIQ
NDILKEYAARGTYIFPPRPSMRLITDTFAYCAERIPRWNTISISGYHIREAGATAVQELAFTLANGIAYVQGAIDAGLHV
DEFGPRLSFFFNAHNNFFEEIAKFRAARRMWATIMRERFGATDERALTLRFHTQTGGSTLTSQQPLNNVVRVTLQALAAV
MGGTQSLHTNGFDEALSLPTTEAATLALRTQQVIAYESGVTASADPLGGSYLVESMTDEIEQRALELIRHIDDLGGAVRA
VEDGFVQAQIMDTAYDYQNRVESGEQLVIGVNKFVTDDAPVPIFKPNEAVAAEQCAKLAKLRAERDQAAVDAALADLQRA
AEGSANVLYPMREALSQRATVGEVCGVLRRIWGEYRPDVAI
>Mature_520_residues
GSQITTDAGIPVEPIYRDNDRQPEPDAGQFPYTRGIYPTMYRGRLWTMRQYAGFASAHETNERFRYLLNQGQMGLSVAFD
LPTQLGLDSDDPRAEGEVGKVGVAIDSIDDMATLFAGIPLDKVSTSMTINAPASVLLLLYELVGAQQGAASKTLTGTIQN
DILKEYAARGTYIFPPRPSMRLITDTFAYCAERIPRWNTISISGYHIREAGATAVQELAFTLANGIAYVQGAIDAGLHVD
EFGPRLSFFFNAHNNFFEEIAKFRAARRMWATIMRERFGATDERALTLRFHTQTGGSTLTSQQPLNNVVRVTLQALAAVM
GGTQSLHTNGFDEALSLPTTEAATLALRTQQVIAYESGVTASADPLGGSYLVESMTDEIEQRALELIRHIDDLGGAVRAV
EDGFVQAQIMDTAYDYQNRVESGEQLVIGVNKFVTDDAPVPIFKPNEAVAAEQCAKLAKLRAERDQAAVDAALADLQRAA
EGSANVLYPMREALSQRATVGEVCGVLRRIWGEYRPDVAI

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=515, Percent_Identity=48.1553398058252, Blast_Score=500, Evalue=1e-141,
Organism=Escherichia coli, GI1789284, Length=515, Percent_Identity=48.5436893203884, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI25144500, Length=517, Percent_Identity=48.5493230174081, Blast_Score=519, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 56967; Mature: 56836

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSQITTDAGIPVEPIYRDNDRQPEPDAGQFPYTRGIYPTMYRGRLWTMRQYAGFASAHE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCHHHHH
TNERFRYLLNQGQMGLSVAFDLPTQLGLDSDDPRAEGEVGKVGVAIDSIDDMATLFAGIP
HHHHHHHHHHCCCCCEEEEEECCHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCC
LDKVSTSMTINAPASVLLLLYELVGAQQGAASKTLTGTIQNDILKEYAARGTYIFPPRPS
HHHCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEECCCCC
MRLITDTFAYCAERIPRWNTISISGYHIREAGATAVQELAFTLANGIAYVQGAIDAGLHV
HHHHHHHHHHHHHHCCCCCEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DEFGPRLSFFFNAHNNFFEEIAKFRAARRMWATIMRERFGATDERALTLRFHTQTGGSTL
HHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCC
TSQQPLNNVVRVTLQALAAVMGGTQSLHTNGFDEALSLPTTEAATLALRTQQVIAYESGV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCC
TASADPLGGSYLVESMTDEIEQRALELIRHIDDLGGAVRAVEDGFVQAQIMDTAYDYQNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
VESGEQLVIGVNKFVTDDAPVPIFKPNEAVAAEQCAKLAKLRAERDQAAVDAALADLQRA
HCCCCEEEEECHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEGSANVLYPMREALSQRATVGEVCGVLRRIWGEYRPDVAI
HCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GSQITTDAGIPVEPIYRDNDRQPEPDAGQFPYTRGIYPTMYRGRLWTMRQYAGFASAHE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCHHHHH
TNERFRYLLNQGQMGLSVAFDLPTQLGLDSDDPRAEGEVGKVGVAIDSIDDMATLFAGIP
HHHHHHHHHHCCCCCEEEEEECCHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCC
LDKVSTSMTINAPASVLLLLYELVGAQQGAASKTLTGTIQNDILKEYAARGTYIFPPRPS
HHHCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEECCCCC
MRLITDTFAYCAERIPRWNTISISGYHIREAGATAVQELAFTLANGIAYVQGAIDAGLHV
HHHHHHHHHHHHHHCCCCCEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DEFGPRLSFFFNAHNNFFEEIAKFRAARRMWATIMRERFGATDERALTLRFHTQTGGSTL
HHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCC
TSQQPLNNVVRVTLQALAAVMGGTQSLHTNGFDEALSLPTTEAATLALRTQQVIAYESGV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCC
TASADPLGGSYLVESMTDEIEQRALELIRHIDDLGGAVRAVEDGFVQAQIMDTAYDYQNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
VESGEQLVIGVNKFVTDDAPVPIFKPNEAVAAEQCAKLAKLRAERDQAAVDAALADLQRA
HCCCCEEEEECHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEGSANVLYPMREALSQRATVGEVCGVLRRIWGEYRPDVAI
HCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]