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The map label for this gene is galE [C]

Identifier: 159900948

GI number: 159900948

Start: 5675620

End: 5676573

Strand: Direct

Name: galE [C]

Synonym: Haur_4435

Alternate gene names: 159900948

Gene position: 5675620-5676573 (Clockwise)

Preceding gene: 159900947

Following gene: 159900949

Centisome position: 89.43

GC content: 49.79

Gene sequence:

>954_bases
ATGACATATTTAGTAACGGGTGGTGCGGGTTTTATTGGCTCGCATTTGTGCGAAACACTGTTGCAGCGTGGCGAACGTGT
CATTGCATTCGATAATTTCAACGATTATTACAGCCCTGAACGCAAACGCCGTAACGTAGCAGGTTTGCTTGATCATCTCA
ATTTTGTGCTGTGGGAAGGCGATTTGCGCGATCCGGCCAGTTTATTGGCCTTGTTTGAGCAACATCGGCCAAGCCATGTA
GCGCATTTGGCAGGCATGGCCAACCCGCGCTATTCGTTGCAATACCCCGCTTTGTATAGCGCCGTCAATGTTGAAGGCTC
GGTCAATGTCTGGCAAGCGGCGATTCAATATGGCATTCAGGCCTTTGTTCAAGCCTCAACATCGTCGGTGTATGGGCTTG
CACCAACCCCATGGCACGAAGAATTAGCCACCGATCGACCATTATCGCCCTATGCTGCTACCAAAAAAGCCTCAGAACTG
CTGGCCTATACCTTCCACTACCAAACCCAAATTCCGACCCGCGTGGTGCGCTTTTTCACGGTCTATGGCCCCAAAGGCCG
CCCCGATATGACCCCAACAATTTTTGTTGAGGCCATGCGCAAGCAAGAGCCAATTGTGCTTTACAATGGCGGGGTTGATG
TTTACCGCGATTGGACCTACGTTGATGATATTGTTTCAGGGGTGATTGCGGTCCTCGATAGCGATCGCGCCTTTGATATT
TTCAATTTAGGCAACTCAACTCCGGTGATGCTACGCAGTTTTATTGATACGCTGCAAGCAATTACTGGCTTGAATGCAAT
TATTGAAGCCAAGCCTTTATCATCTGCCGACCCGCCAATTACCTTTGCCGACACCTCCAAGGCGCAACAATTGCTGGGTT
GGAAGCCAACCATCGATATCGAGGATGGCCTAGAGCGCTATTGGCATTGGTATAAAACCGAGTACGATTGCTAG

Upstream 100 bases:

>100_bases
ATAATGTTTTCGATAATCTCCATGTGTTGAATTTTGGAATTCATTTGAGCGCAGTGTGGGCCTTATGTGTGGTTCTGACA
CAGCGCCAAGGGTGGCGTGC

Downstream 100 bases:

>100_bases
ATCGGCATCGTGTTAGAAACAAACTGGATCATTATGAAATCGCAAACGCAAGCAAATCCTGCTAGTTTACGCCCTGAGCA
AACCAACCAAGGCTTGCCGC

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTYLVTGGAGFIGSHLCETLLQRGERVIAFDNFNDYYSPERKRRNVAGLLDHLNFVLWEGDLRDPASLLALFEQHRPSHV
AHLAGMANPRYSLQYPALYSAVNVEGSVNVWQAAIQYGIQAFVQASTSSVYGLAPTPWHEELATDRPLSPYAATKKASEL
LAYTFHYQTQIPTRVVRFFTVYGPKGRPDMTPTIFVEAMRKQEPIVLYNGGVDVYRDWTYVDDIVSGVIAVLDSDRAFDI
FNLGNSTPVMLRSFIDTLQAITGLNAIIEAKPLSSADPPITFADTSKAQQLLGWKPTIDIEDGLERYWHWYKTEYDC

Sequences:

>Translated_317_residues
MTYLVTGGAGFIGSHLCETLLQRGERVIAFDNFNDYYSPERKRRNVAGLLDHLNFVLWEGDLRDPASLLALFEQHRPSHV
AHLAGMANPRYSLQYPALYSAVNVEGSVNVWQAAIQYGIQAFVQASTSSVYGLAPTPWHEELATDRPLSPYAATKKASEL
LAYTFHYQTQIPTRVVRFFTVYGPKGRPDMTPTIFVEAMRKQEPIVLYNGGVDVYRDWTYVDDIVSGVIAVLDSDRAFDI
FNLGNSTPVMLRSFIDTLQAITGLNAIIEAKPLSSADPPITFADTSKAQQLLGWKPTIDIEDGLERYWHWYKTEYDC
>Mature_316_residues
TYLVTGGAGFIGSHLCETLLQRGERVIAFDNFNDYYSPERKRRNVAGLLDHLNFVLWEGDLRDPASLLALFEQHRPSHVA
HLAGMANPRYSLQYPALYSAVNVEGSVNVWQAAIQYGIQAFVQASTSSVYGLAPTPWHEELATDRPLSPYAATKKASELL
AYTFHYQTQIPTRVVRFFTVYGPKGRPDMTPTIFVEAMRKQEPIVLYNGGVDVYRDWTYVDDIVSGVIAVLDSDRAFDIF
NLGNSTPVMLRSFIDTLQAITGLNAIIEAKPLSSADPPITFADTSKAQQLLGWKPTIDIEDGLERYWHWYKTEYDC

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=320, Percent_Identity=29.0625, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI7657641, Length=324, Percent_Identity=25, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI56237023, Length=335, Percent_Identity=26.2686567164179, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI56118217, Length=335, Percent_Identity=26.2686567164179, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI189083684, Length=335, Percent_Identity=26.2686567164179, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1786974, Length=337, Percent_Identity=27.893175074184, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788353, Length=344, Percent_Identity=27.906976744186, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI48994969, Length=336, Percent_Identity=26.7857142857143, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788366, Length=349, Percent_Identity=25.7879656160458, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=324, Percent_Identity=28.0864197530864, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982038, Length=339, Percent_Identity=26.8436578171091, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71982035, Length=338, Percent_Identity=27.2189349112426, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI115532424, Length=315, Percent_Identity=27.3015873015873, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=325, Percent_Identity=22.7692307692308, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=335, Percent_Identity=23.5820895522388, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=335, Percent_Identity=22.6865671641791, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=335, Percent_Identity=22.6865671641791, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=335, Percent_Identity=22.6865671641791, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=335, Percent_Identity=22.6865671641791, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=335, Percent_Identity=22.6865671641791, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=339, Percent_Identity=25.0737463126844, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356223, Length=321, Percent_Identity=32.0872274143302, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19923002, Length=337, Percent_Identity=27.0029673590504, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 35671; Mature: 35539

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYLVTGGAGFIGSHLCETLLQRGERVIAFDNFNDYYSPERKRRNVAGLLDHLNFVLWEG
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEC
DLRDPASLLALFEQHRPSHVAHLAGMANPRYSLQYPALYSAVNVEGSVNVWQAAIQYGIQ
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCHHHHEECCCCCHHHHHHHHHHHHH
AFVQASTSSVYGLAPTPWHEELATDRPLSPYAATKKASELLAYTFHYQTQIPTRVVRFFT
HHHHHCCCCEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHEEEECCCCHHHHHHHHH
VYGPKGRPDMTPTIFVEAMRKQEPIVLYNGGVDVYRDWTYVDDIVSGVIAVLDSDRAFDI
EECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEECHHHHHHHHHHHHHHHCCCCCEEE
FNLGNSTPVMLRSFIDTLQAITGLNAIIEAKPLSSADPPITFADTSKAQQLLGWKPTIDI
EECCCCCHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCEEECCCHHHHHHHCCCCCCCH
EDGLERYWHWYKTEYDC
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TYLVTGGAGFIGSHLCETLLQRGERVIAFDNFNDYYSPERKRRNVAGLLDHLNFVLWEG
EEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEC
DLRDPASLLALFEQHRPSHVAHLAGMANPRYSLQYPALYSAVNVEGSVNVWQAAIQYGIQ
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCHHHHEECCCCCHHHHHHHHHHHHH
AFVQASTSSVYGLAPTPWHEELATDRPLSPYAATKKASELLAYTFHYQTQIPTRVVRFFT
HHHHHCCCCEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHEEEECCCCHHHHHHHHH
VYGPKGRPDMTPTIFVEAMRKQEPIVLYNGGVDVYRDWTYVDDIVSGVIAVLDSDRAFDI
EECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEECHHHHHHHHHHHHHHHCCCCCEEE
FNLGNSTPVMLRSFIDTLQAITGLNAIIEAKPLSSADPPITFADTSKAQQLLGWKPTIDI
EECCCCCHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCEEECCCHHHHHHHCCCCCCCH
EDGLERYWHWYKTEYDC
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]