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The map label for this gene is dnaB [H]

Identifier: 159900046

GI number: 159900046

Start: 4442283

End: 4444064

Strand: Direct

Name: dnaB [H]

Synonym: Haur_3529

Alternate gene names: 159900046

Gene position: 4442283-4444064 (Clockwise)

Preceding gene: 159900045

Following gene: 159900047

Centisome position: 69.99

GC content: 49.27

Gene sequence:

>1782_bases
ATGGATAAGTCGTTACCCGCCGATATTAACGCCGAACGTGCCACCCTTGGTTCGATTTTGCTTGATCGTGATGCAATTAT
TCCAGTTGCACCTTGGCTTGCTAGCGACTATTTTTATCTTGAAAAGCATGGCTTGGTTTTTAATGCCCAAGTGGCTTGTT
ACAACCGCCGTGTGCCACCCGATTTGGTCAATGTTACCGATGAGCTGCGCCGCAAAGATCAATTAGAGGTCGTCGGCGGG
GTTCAGTATTTGCTTGAGCTTTCGAATAGTGTGCCAACCTCGGTGCACGTCGAATATTATGCCCGCATCGTTGAACGCAC
CGCACTATTGCGCCGTTTGATCATCGCTGGCGGCAAGATTGCGGCCCTCGGTTATGACGAAACTCAAGAGCTTGAGGCTG
CGCTCGATCAGGCCGAATCAGAATTATTTGCGGTTTCGCAGCGGCGTACCGCCGATGGCTTTATTCATATCGGTGCAGTT
GTCGATAGTTTCTTCGAGCAAATTAGCCAAATGCAGGAGCGCGGCGGCGAAGTTGTGGGCCTCAAAACAGGCTTTACCGA
TTTTGATAAATTAACTGGTGGCTTGCAACGCTCCGACTTGTTGATTTTGGCGGCGCGGCCAGCCACTGGCAAAACCAGCT
TGGCCTTGAATATTGCCTACAACGCCGCCAAGGAATCTGAGGCCTGCGTGGCGATTTTTAGCCTTGAAATGAGTCGCGAT
CAGCTGATGCAGCGGATTTTGGCCACCGAAACTGGCGTGGATATGCAAAAGCTGAGGACTGGCCAAATTCGCGATAGTGA
TTTGCAGTTGCTGACCGAGGCGCTTGGTAAACTCTCCACCATGTCAATTTATATCGATGATTCACCTGGAGCCAGCATTA
TGGATGTGCGCTCCAAATGTCGGCGCTTGCAAGCTGAGGCAGGCATCGATCTGATTATCATCGATTATTTGCAGTTGATG
CAGGGTGGCGGCAAACGCGATGGCAACCGTGTCCAGGAAATTAGCGAAATTAGCCGTGGACTCAAGGCCTTGGCCCGCGA
AATCAATGTGCCAGTGATTGCGCTTTCGCAGCTTTCGCGGGCCGTCGAAAGCCGCACCACCCACGTTCCCATGCTCTCCG
ATTTGCGCGAATCAGGGTGTCTCACAGGCGATACCCAAATCTATTTGCCTGATCAAGGTCATTATGTCCGAATCGATCAA
TTAGTTGGGCAACAAGGCTTTAATGTTCTAGCGCTGAATCAAGCAACTTGGAAACTTGAATCATGGCCTGTTACCCATGC
CTTTACAACTGGCACAAAACCTGTTTTTAAACTCCGTACTAAGCTAGGGCGCAGTATTCGCGCCACGGTTAACCATAAAT
TCTTAACCATTGATGGTTGGCAACGGCTTGATCAGTTGCATCAAACCAGCCAAATTGCCATCTCACAGGAAATTGCCCAT
GAATTGGGTAGCAACAGCCAACCAACCGCGATTGAATGGGAAACAATCATTAGCATCGAGCCTGATGGCGTTGAGCCAGT
TTATGATTTGACGGTGGATACGCTCCATAATTTTGTGGCAAATAATATCATTGTGCATAACAGTATCGAGCAAGATGCGG
ATATCGTGATGTTTATTTATCGTGAAGAGCTGTATGATCCCGAAACTGATAAGAAGGGCATCGCCGAAATTCACCTAGCC
AAGCACCGTAACGGCCCAACCGGGATCGTGCCTTTGCGCTTTTTTCGCTCGACAACGAAATTTTCCAATTTAGAGACCTA
TCGCCAACCTGAAGGCTATTAG

Upstream 100 bases:

>100_bases
CGCCAATGAGCGCGGCATCAGTGCTTTAGCCGTTGGTTTCCACAGCTTTTCGACTGTGTATCCACCCGTTATCCCAACAT
TTTCCACATCCGAGGTCGCT

Downstream 100 bases:

>100_bases
TGCCCTCACCCCCTAGCCTTACAAGAGTAGGTTTTAACATTCCCTCACCCCCCAACCCCTCTCCCACTGCGGCGGGAGAG
GGGCGTTCCAATCATCATGA

Product: replicative DNA helicase

Products: NA

Alternate protein names: Asp dnaB intein [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MDKSLPADINAERATLGSILLDRDAIIPVAPWLASDYFYLEKHGLVFNAQVACYNRRVPPDLVNVTDELRRKDQLEVVGG
VQYLLELSNSVPTSVHVEYYARIVERTALLRRLIIAGGKIAALGYDETQELEAALDQAESELFAVSQRRTADGFIHIGAV
VDSFFEQISQMQERGGEVVGLKTGFTDFDKLTGGLQRSDLLILAARPATGKTSLALNIAYNAAKESEACVAIFSLEMSRD
QLMQRILATETGVDMQKLRTGQIRDSDLQLLTEALGKLSTMSIYIDDSPGASIMDVRSKCRRLQAEAGIDLIIIDYLQLM
QGGGKRDGNRVQEISEISRGLKALAREINVPVIALSQLSRAVESRTTHVPMLSDLRESGCLTGDTQIYLPDQGHYVRIDQ
LVGQQGFNVLALNQATWKLESWPVTHAFTTGTKPVFKLRTKLGRSIRATVNHKFLTIDGWQRLDQLHQTSQIAISQEIAH
ELGSNSQPTAIEWETIISIEPDGVEPVYDLTVDTLHNFVANNIIVHNSIEQDADIVMFIYREELYDPETDKKGIAEIHLA
KHRNGPTGIVPLRFFRSTTKFSNLETYRQPEGY

Sequences:

>Translated_593_residues
MDKSLPADINAERATLGSILLDRDAIIPVAPWLASDYFYLEKHGLVFNAQVACYNRRVPPDLVNVTDELRRKDQLEVVGG
VQYLLELSNSVPTSVHVEYYARIVERTALLRRLIIAGGKIAALGYDETQELEAALDQAESELFAVSQRRTADGFIHIGAV
VDSFFEQISQMQERGGEVVGLKTGFTDFDKLTGGLQRSDLLILAARPATGKTSLALNIAYNAAKESEACVAIFSLEMSRD
QLMQRILATETGVDMQKLRTGQIRDSDLQLLTEALGKLSTMSIYIDDSPGASIMDVRSKCRRLQAEAGIDLIIIDYLQLM
QGGGKRDGNRVQEISEISRGLKALAREINVPVIALSQLSRAVESRTTHVPMLSDLRESGCLTGDTQIYLPDQGHYVRIDQ
LVGQQGFNVLALNQATWKLESWPVTHAFTTGTKPVFKLRTKLGRSIRATVNHKFLTIDGWQRLDQLHQTSQIAISQEIAH
ELGSNSQPTAIEWETIISIEPDGVEPVYDLTVDTLHNFVANNIIVHNSIEQDADIVMFIYREELYDPETDKKGIAEIHLA
KHRNGPTGIVPLRFFRSTTKFSNLETYRQPEGY
>Mature_593_residues
MDKSLPADINAERATLGSILLDRDAIIPVAPWLASDYFYLEKHGLVFNAQVACYNRRVPPDLVNVTDELRRKDQLEVVGG
VQYLLELSNSVPTSVHVEYYARIVERTALLRRLIIAGGKIAALGYDETQELEAALDQAESELFAVSQRRTADGFIHIGAV
VDSFFEQISQMQERGGEVVGLKTGFTDFDKLTGGLQRSDLLILAARPATGKTSLALNIAYNAAKESEACVAIFSLEMSRD
QLMQRILATETGVDMQKLRTGQIRDSDLQLLTEALGKLSTMSIYIDDSPGASIMDVRSKCRRLQAEAGIDLIIIDYLQLM
QGGGKRDGNRVQEISEISRGLKALAREINVPVIALSQLSRAVESRTTHVPMLSDLRESGCLTGDTQIYLPDQGHYVRIDQ
LVGQQGFNVLALNQATWKLESWPVTHAFTTGTKPVFKLRTKLGRSIRATVNHKFLTIDGWQRLDQLHQTSQIAISQEIAH
ELGSNSQPTAIEWETIISIEPDGVEPVYDLTVDTLHNFVANNIIVHNSIEQDADIVMFIYREELYDPETDKKGIAEIHLA
KHRNGPTGIVPLRFFRSTTKFSNLETYRQPEGY

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=393, Percent_Identity=42.4936386768448, Blast_Score=281, Evalue=6e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 66068; Mature: 66068

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50817 INTEIN_N_TER ; PS50818 INTEIN_C_TER ; PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSLPADINAERATLGSILLDRDAIIPVAPWLASDYFYLEKHGLVFNAQVACYNRRVPP
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHCCEEEEECCCEEEEEEEEEECCCCCH
DLVNVTDELRRKDQLEVVGGVQYLLELSNSVPTSVHVEYYARIVERTALLRRLIIAGGKI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCE
AALGYDETQELEAALDQAESELFAVSQRRTADGFIHIGAVVDSFFEQISQMQERGGEVVG
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEE
LKTGFTDFDKLTGGLQRSDLLILAARPATGKTSLALNIAYNAAKESEACVAIFSLEMSRD
EECCCCCHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCHH
QLMQRILATETGVDMQKLRTGQIRDSDLQLLTEALGKLSTMSIYIDDSPGASIMDVRSKC
HHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHH
RRLQAEAGIDLIIIDYLQLMQGGGKRDGNRVQEISEISRGLKALAREINVPVIALSQLSR
HHHHHHCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
AVESRTTHVPMLSDLRESGCLTGDTQIYLPDQGHYVRIDQLVGQQGFNVLALNQATWKLE
HHHHCCCCCCHHHHHHHCCCCCCCCEEEECCCCCEEEHHHHHCCCCCCEEEECCCEEEEE
SWPVTHAFTTGTKPVFKLRTKLGRSIRATVNHKFLTIDGWQRLDQLHQTSQIAISQEIAH
CCCEEEEECCCCCHHHHHHHHHCCCEEEEECEEEEEECHHHHHHHHHHHHHHHHHHHHHH
ELGSNSQPTAIEWETIISIEPDGVEPVYDLTVDTLHNFVANNIIVHNSIEQDADIVMFIY
HHCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEE
REELYDPETDKKGIAEIHLAKHRNGPTGIVPLRFFRSTTKFSNLETYRQPEGY
EHHHCCCCCCCCCHHEEEEHHCCCCCCCEEEHHHHHHHHCCCCCHHCCCCCCC
>Mature Secondary Structure
MDKSLPADINAERATLGSILLDRDAIIPVAPWLASDYFYLEKHGLVFNAQVACYNRRVPP
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHCCEEEEECCCEEEEEEEEEECCCCCH
DLVNVTDELRRKDQLEVVGGVQYLLELSNSVPTSVHVEYYARIVERTALLRRLIIAGGKI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCE
AALGYDETQELEAALDQAESELFAVSQRRTADGFIHIGAVVDSFFEQISQMQERGGEVVG
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEE
LKTGFTDFDKLTGGLQRSDLLILAARPATGKTSLALNIAYNAAKESEACVAIFSLEMSRD
EECCCCCHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCHH
QLMQRILATETGVDMQKLRTGQIRDSDLQLLTEALGKLSTMSIYIDDSPGASIMDVRSKC
HHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHH
RRLQAEAGIDLIIIDYLQLMQGGGKRDGNRVQEISEISRGLKALAREINVPVIALSQLSR
HHHHHHCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
AVESRTTHVPMLSDLRESGCLTGDTQIYLPDQGHYVRIDQLVGQQGFNVLALNQATWKLE
HHHHCCCCCCHHHHHHHCCCCCCCCEEEECCCCCEEEHHHHHCCCCCCEEEECCCEEEEE
SWPVTHAFTTGTKPVFKLRTKLGRSIRATVNHKFLTIDGWQRLDQLHQTSQIAISQEIAH
CCCEEEEECCCCCHHHHHHHHHCCCEEEEECEEEEEECHHHHHHHHHHHHHHHHHHHHHH
ELGSNSQPTAIEWETIISIEPDGVEPVYDLTVDTLHNFVANNIIVHNSIEQDADIVMFIY
HHCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEE
REELYDPETDKKGIAEIHLAKHRNGPTGIVPLRFFRSTTKFSNLETYRQPEGY
EHHHCCCCCCCCCHHEEEEHHCCCCCCCEEEHHHHHHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]