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The map label for this gene is trmE [H]

Identifier: 159899958

GI number: 159899958

Start: 4328544

End: 4329908

Strand: Direct

Name: trmE [H]

Synonym: Haur_3441

Alternate gene names: 159899958

Gene position: 4328544-4329908 (Clockwise)

Preceding gene: 159899957

Following gene: 159899959

Centisome position: 68.2

GC content: 54.58

Gene sequence:

>1365_bases
ATGCTCTATGCTGATACGATCGCTGCGTTGGCTACCCCACCTGGCGTTGGTGGGGTTGGAATTATTCGGGTCAGCGGCCC
TCAAAGCTTAGCTATCGCCCAAGCCTTGATCAAACCGCGCCGCAAAGGCGTTTGGCGCTCCTACCAAATGCGCTATGGCC
ATGTGCTCGATGAGCATGGACAAATTGTCGATGAGGTGTTGGCGGTCTTTTTTAAGGGGCCACGCTCATTCACCGGCGAA
GATATTTTGGAGATTCACTGCCACGGCGGCCCGCTGCCCTTGCGCCGAACCCTGACCTTGGCTCTAGCTCATGGCGCTCG
CTTAGCCAACCCAGGCGAATTTAGCTTACGCGCTTTTGCCAATGGCCGCATCGATTTAGTCCAAGCTGAGGCAACTCTCG
ATGTGATCGAAGCCCAAACCAACTTGGGGCTTAGCTTGGCGCTCGATCAGCTGGGCGGCGGGCTTTCGCGTGATCTGCGC
ACATTGCGCGAGCAGCTGATGTATCCCTTGGCCTATGTCACAGCCCTCACCGATTTTCCCGAAGATGATGTGCCCAGCGA
GGAATTACAACAGCCCTTGCAGCAAGCCCAAAGCCTGCTCAGCCAACTGCTAGCTGGGGCCGATCAAGGTGTGGTTGTGC
GCGAAGGGGCACGCGCTGCCTTGGTTGGTCGGCCCAACGCTGGCAAATCGAGCTTGATGAATGCCCTGTTGCGCACCGAA
CGAGCCATCGTCACCGCCATTCCCGGCACAACCCGCGATACCTTGGAAGAAACCGCCAATCTGGGCGGAATTCCAGTGGT
GCTGATTGATACTGCGGGGATTACTGAAACCGATGATGTAGTCGAACGAATTGGAGTCGAACGCTCACGCGCCGCTCTCA
GCAAAGCCGATTTAGTGTTGCTGTTGATCGATGGTTCGCAGCCGTTAAGCCCAGAAGATTTGGCAATTGCCCAATTAACT
CATGAACGACCCACAATCGTGATTGCTACCAAAGCCGATTTAGGCCAACACGCTGATTTAACCGCGCTGACTCAAACTCA
CCCCAAGCTCCGTGGCAGCATCGCAATCTCATCGCAGGCTGGCACAGGCTTGGATTATTTAGGTACAATGGTAGCCGAAC
AGTTGCTTGGTGGTTTGCCGCTCAGTGATGCCCGCTTAGTCACCAATCCACGCCACCGCGAGGCTTTGCGCCGTGCCAAT
GCCGCCTTAGAACAAGCCATCCAAGGTTTGCGCGAAGGCCGCCCAGTTGATTTGATTGCGATCGATTTGCATGAGGCGAT
CGCTAGTTTAGGCGAAATTACTGGCGAAACTGTCGAACATGATTTATTGAATATGATCTTTAGTCGCTTTTGTATTGGCA
AATAA

Upstream 100 bases:

>100_bases
CACCCAGTTTTAACCATAGTTTTGCGCCAACCTGTGGCTTTTGCCATGTCCAAAGCATGCCCGCAACTCTGCCCTTGACC
AGCACATCGAGGTTGCGCTA

Downstream 100 bases:

>100_bases
TCACGCCCACAAGGAGCCACCCGTGCCCGATTCAAAGCAGCTACCCGTCGAACGTGCCAAATTGTATGCACGAATTATGC
TCGGCATTACCGCCTTGTTT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MLYADTIAALATPPGVGGVGIIRVSGPQSLAIAQALIKPRRKGVWRSYQMRYGHVLDEHGQIVDEVLAVFFKGPRSFTGE
DILEIHCHGGPLPLRRTLTLALAHGARLANPGEFSLRAFANGRIDLVQAEATLDVIEAQTNLGLSLALDQLGGGLSRDLR
TLREQLMYPLAYVTALTDFPEDDVPSEELQQPLQQAQSLLSQLLAGADQGVVVREGARAALVGRPNAGKSSLMNALLRTE
RAIVTAIPGTTRDTLEETANLGGIPVVLIDTAGITETDDVVERIGVERSRAALSKADLVLLLIDGSQPLSPEDLAIAQLT
HERPTIVIATKADLGQHADLTALTQTHPKLRGSIAISSQAGTGLDYLGTMVAEQLLGGLPLSDARLVTNPRHREALRRAN
AALEQAIQGLREGRPVDLIAIDLHEAIASLGEITGETVEHDLLNMIFSRFCIGK

Sequences:

>Translated_454_residues
MLYADTIAALATPPGVGGVGIIRVSGPQSLAIAQALIKPRRKGVWRSYQMRYGHVLDEHGQIVDEVLAVFFKGPRSFTGE
DILEIHCHGGPLPLRRTLTLALAHGARLANPGEFSLRAFANGRIDLVQAEATLDVIEAQTNLGLSLALDQLGGGLSRDLR
TLREQLMYPLAYVTALTDFPEDDVPSEELQQPLQQAQSLLSQLLAGADQGVVVREGARAALVGRPNAGKSSLMNALLRTE
RAIVTAIPGTTRDTLEETANLGGIPVVLIDTAGITETDDVVERIGVERSRAALSKADLVLLLIDGSQPLSPEDLAIAQLT
HERPTIVIATKADLGQHADLTALTQTHPKLRGSIAISSQAGTGLDYLGTMVAEQLLGGLPLSDARLVTNPRHREALRRAN
AALEQAIQGLREGRPVDLIAIDLHEAIASLGEITGETVEHDLLNMIFSRFCIGK
>Mature_454_residues
MLYADTIAALATPPGVGGVGIIRVSGPQSLAIAQALIKPRRKGVWRSYQMRYGHVLDEHGQIVDEVLAVFFKGPRSFTGE
DILEIHCHGGPLPLRRTLTLALAHGARLANPGEFSLRAFANGRIDLVQAEATLDVIEAQTNLGLSLALDQLGGGLSRDLR
TLREQLMYPLAYVTALTDFPEDDVPSEELQQPLQQAQSLLSQLLAGADQGVVVREGARAALVGRPNAGKSSLMNALLRTE
RAIVTAIPGTTRDTLEETANLGGIPVVLIDTAGITETDDVVERIGVERSRAALSKADLVLLLIDGSQPLSPEDLAIAQLT
HERPTIVIATKADLGQHADLTALTQTHPKLRGSIAISSQAGTGLDYLGTMVAEQLLGGLPLSDARLVTNPRHREALRRAN
AALEQAIQGLREGRPVDLIAIDLHEAIASLGEITGETVEHDLLNMIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=472, Percent_Identity=36.0169491525424, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI193082988, Length=501, Percent_Identity=33.7325349301397, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI193082993, Length=461, Percent_Identity=36.2255965292842, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI2367268, Length=466, Percent_Identity=39.6995708154506, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI87082120, Length=116, Percent_Identity=45.6896551724138, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17507259, Length=460, Percent_Identity=31.304347826087, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=29.8174442190669, Blast_Score=202, Evalue=6e-53,
Organism=Drosophila melanogaster, GI45550813, Length=483, Percent_Identity=33.1262939958592, Blast_Score=205, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 48512; Mature: 48512

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYADTIAALATPPGVGGVGIIRVSGPQSLAIAQALIKPRRKGVWRSYQMRYGHVLDEHG
CCCHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHH
QIVDEVLAVFFKGPRSFTGEDILEIHCHGGPLPLRRTLTLALAHGARLANPGEFSLRAFA
HHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEC
NGRIDLVQAEATLDVIEAQTNLGLSLALDQLGGGLSRDLRTLREQLMYPLAYVTALTDFP
CCCEEEEEEHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EDDVPSEELQQPLQQAQSLLSQLLAGADQGVVVREGARAALVGRPNAGKSSLMNALLRTE
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHC
RAIVTAIPGTTRDTLEETANLGGIPVVLIDTAGITETDDVVERIGVERSRAALSKADLVL
CCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCCEEE
LLIDGSQPLSPEDLAIAQLTHERPTIVIATKADLGQHADLTALTQTHPKLRGSIAISSQA
EEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCEEEEECCC
GTGLDYLGTMVAEQLLGGLPLSDARLVTNPRHREALRRANAALEQAIQGLREGRPVDLIA
CCCHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IDLHEAIASLGEITGETVEHDLLNMIFSRFCIGK
EEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLYADTIAALATPPGVGGVGIIRVSGPQSLAIAQALIKPRRKGVWRSYQMRYGHVLDEHG
CCCHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHH
QIVDEVLAVFFKGPRSFTGEDILEIHCHGGPLPLRRTLTLALAHGARLANPGEFSLRAFA
HHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEC
NGRIDLVQAEATLDVIEAQTNLGLSLALDQLGGGLSRDLRTLREQLMYPLAYVTALTDFP
CCCEEEEEEHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EDDVPSEELQQPLQQAQSLLSQLLAGADQGVVVREGARAALVGRPNAGKSSLMNALLRTE
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHC
RAIVTAIPGTTRDTLEETANLGGIPVVLIDTAGITETDDVVERIGVERSRAALSKADLVL
CCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCCEEE
LLIDGSQPLSPEDLAIAQLTHERPTIVIATKADLGQHADLTALTQTHPKLRGSIAISSQA
EEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCEEEEECCC
GTGLDYLGTMVAEQLLGGLPLSDARLVTNPRHREALRRANAALEQAIQGLREGRPVDLIA
CCCHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IDLHEAIASLGEITGETVEHDLLNMIFSRFCIGK
EEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA