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The map label for this gene is wcaK [H]

Identifier: 159899873

GI number: 159899873

Start: 4233826

End: 4235133

Strand: Direct

Name: wcaK [H]

Synonym: Haur_3356

Alternate gene names: 159899873

Gene position: 4233826-4235133 (Clockwise)

Preceding gene: 159899872

Following gene: 159899874

Centisome position: 66.71

GC content: 51.99

Gene sequence:

>1308_bases
ATGAAGGTTTTATTATTGAATGCCCATTCGCCGCAAAATGCTGGCGATTTGGCCTTACTTGAGCAATCGTTGGCCCATTT
ACGAGCCGCGTTTCCCCATGCCGATTTGAGCTATGTGATCAATCAGCCTGATCCGCCGCAATGGCTGCCTGCTGATGTTC
CCTATATTTTGTCGATCCATGAGCATAAAACCAATTTGATCAAAGATGCACCCAAGCCACGGCGTAAATGGCTGATGCTG
GGGCTGGCAATCTGGTTGGTTGGCATCAGCCTGATCTATCGCTGGACAGGCATCAAACTCAAGCCAGCCAAAACCAGCGA
TTGGCGTGAATTACTTGAGCATTATTTTGAGGCCGATGTTACGGCAGCGATTGGCGGCGGCTATTTGTATGCGACCAAAG
CCTTCGACCTGAACTACATTTGGGTGTGGTTGGGCGTGGCCTTGCCCGTGCTCATGGGCAAGCCGCTAGTAATGTTGCCG
CAATCGTTTGGGCCGGTCACTGGCAAAATCAACCAACTGCTGTTGGCATGGTTGGTCAATCGCTCAGTCCAAGCCTATGC
CCGCGAAGAACGTTCGCAGCACTATCTCTATAGCATCGGGGTTAATCAATCAGTTCAGGTTGTGCCTGATGTCGCCTTCA
ACTGCCCAACCGTCGAGCCAGCCCAAGCCGAGCACTATTTGGCCCGTTGGTGGCAACCAGCCAAACGCCCTGCTTTAGTC
GTTGGCATCACGGCGATGGATTTTGGCATTCAGCACCCAGGCTCGGGGTTTAGCCATGGCCAACGTTATGAGCAAGCCTT
GCTCGATTTGATTCAACATATCGGCCAGTGTGATGATGTGCATATCTTGCTGTTTGCCCAATGCGTTGGGGCCAGCGAGG
CTGAGGATGATCGCGTGGTGGCGCGGCGCTTAATCGCTCAACTGCCAGCCACTACGCCAATCACTTTTATTGATGATCGC
CTGCAACCAGCCATGCTCAAACAACTCTACAGCCAGATTGATCTACTGATTGCAACGCGTTTGCATTCGGCAATTTTCGC
CTTGAGTACGGCCACACCCAGCTTTGTGATTGGCTATTTGCACAAATCGGCAGGCGTGATGCACATGCTTGGCTTGGCTG
ATTACCAAATTCCGATTGAATCGATCGATAGCAGCAACATTATTAGTGCCTTTGAACAAACCCTCGCTGCCCGTGGCTCG
ATTAAAACCATGATGCGCAGCAGCATTCCCGCCTTGCAAAGCCAACTTGAACGTTTGCCCAAGCAGATTCGGGCGGCGGT
TGGCGATTGGTTACGAGGAGCAAAGTAG

Upstream 100 bases:

>100_bases
ATGGTCTGCATGGCGTAGCGATTATTAACATGATCACCGAAATTGCCCTGTTTATCTCGTATGGTTATGGAGCTTGGCTG
ACCTATAAGAAAGTGATGCG

Downstream 100 bases:

>100_bases
CAAATTGTATGCGTAAAATAACTGTCGCTCAAGTGATTACCGGCTTTGCGAGTGCCGAAGGTGCTGGCGGCTCAGCCTTA
TTTGGCATCGAAGTAGCACG

Product: polysaccharide pyruvyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKVLLLNAHSPQNAGDLALLEQSLAHLRAAFPHADLSYVINQPDPPQWLPADVPYILSIHEHKTNLIKDAPKPRRKWLML
GLAIWLVGISLIYRWTGIKLKPAKTSDWRELLEHYFEADVTAAIGGGYLYATKAFDLNYIWVWLGVALPVLMGKPLVMLP
QSFGPVTGKINQLLLAWLVNRSVQAYAREERSQHYLYSIGVNQSVQVVPDVAFNCPTVEPAQAEHYLARWWQPAKRPALV
VGITAMDFGIQHPGSGFSHGQRYEQALLDLIQHIGQCDDVHILLFAQCVGASEAEDDRVVARRLIAQLPATTPITFIDDR
LQPAMLKQLYSQIDLLIATRLHSAIFALSTATPSFVIGYLHKSAGVMHMLGLADYQIPIESIDSSNIISAFEQTLAARGS
IKTMMRSSIPALQSQLERLPKQIRAAVGDWLRGAK

Sequences:

>Translated_435_residues
MKVLLLNAHSPQNAGDLALLEQSLAHLRAAFPHADLSYVINQPDPPQWLPADVPYILSIHEHKTNLIKDAPKPRRKWLML
GLAIWLVGISLIYRWTGIKLKPAKTSDWRELLEHYFEADVTAAIGGGYLYATKAFDLNYIWVWLGVALPVLMGKPLVMLP
QSFGPVTGKINQLLLAWLVNRSVQAYAREERSQHYLYSIGVNQSVQVVPDVAFNCPTVEPAQAEHYLARWWQPAKRPALV
VGITAMDFGIQHPGSGFSHGQRYEQALLDLIQHIGQCDDVHILLFAQCVGASEAEDDRVVARRLIAQLPATTPITFIDDR
LQPAMLKQLYSQIDLLIATRLHSAIFALSTATPSFVIGYLHKSAGVMHMLGLADYQIPIESIDSSNIISAFEQTLAARGS
IKTMMRSSIPALQSQLERLPKQIRAAVGDWLRGAK
>Mature_435_residues
MKVLLLNAHSPQNAGDLALLEQSLAHLRAAFPHADLSYVINQPDPPQWLPADVPYILSIHEHKTNLIKDAPKPRRKWLML
GLAIWLVGISLIYRWTGIKLKPAKTSDWRELLEHYFEADVTAAIGGGYLYATKAFDLNYIWVWLGVALPVLMGKPLVMLP
QSFGPVTGKINQLLLAWLVNRSVQAYAREERSQHYLYSIGVNQSVQVVPDVAFNCPTVEPAQAEHYLARWWQPAKRPALV
VGITAMDFGIQHPGSGFSHGQRYEQALLDLIQHIGQCDDVHILLFAQCVGASEAEDDRVVARRLIAQLPATTPITFIDDR
LQPAMLKQLYSQIDLLIATRLHSAIFALSTATPSFVIGYLHKSAGVMHMLGLADYQIPIESIDSSNIISAFEQTLAARGS
IKTMMRSSIPALQSQLERLPKQIRAAVGDWLRGAK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2327

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide pyruvyl transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788358, Length=442, Percent_Identity=24.6606334841629, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007345 [H]

Pfam domain/function: PF04230 PS_pyruv_trans [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 48436; Mature: 48436

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLLNAHSPQNAGDLALLEQSLAHLRAAFPHADLSYVINQPDPPQWLPADVPYILSIH
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCCCCEEEEH
EHKTNLIKDAPKPRRKWLMLGLAIWLVGISLIYRWTGIKLKPAKTSDWRELLEHYFEADV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCC
TAAIGGGYLYATKAFDLNYIWVWLGVALPVLMGKPLVMLPQSFGPVTGKINQLLLAWLVN
CEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHH
RSVQAYAREERSQHYLYSIGVNQSVQVVPDVAFNCPTVEPAQAEHYLARWWQPAKRPALV
HHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEE
VGITAMDFGIQHPGSGFSHGQRYEQALLDLIQHIGQCDDVHILLFAQCVGASEAEDDRVV
EEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHH
ARRLIAQLPATTPITFIDDRLQPAMLKQLYSQIDLLIATRLHSAIFALSTATPSFVIGYL
HHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HKSAGVMHMLGLADYQIPIESIDSSNIISAFEQTLAARGSIKTMMRSSIPALQSQLERLP
HHHCCHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHH
KQIRAAVGDWLRGAK
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVLLLNAHSPQNAGDLALLEQSLAHLRAAFPHADLSYVINQPDPPQWLPADVPYILSIH
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCCCCEEEEH
EHKTNLIKDAPKPRRKWLMLGLAIWLVGISLIYRWTGIKLKPAKTSDWRELLEHYFEADV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCC
TAAIGGGYLYATKAFDLNYIWVWLGVALPVLMGKPLVMLPQSFGPVTGKINQLLLAWLVN
CEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHH
RSVQAYAREERSQHYLYSIGVNQSVQVVPDVAFNCPTVEPAQAEHYLARWWQPAKRPALV
HHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEE
VGITAMDFGIQHPGSGFSHGQRYEQALLDLIQHIGQCDDVHILLFAQCVGASEAEDDRVV
EEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHH
ARRLIAQLPATTPITFIDDRLQPAMLKQLYSQIDLLIATRLHSAIFALSTATPSFVIGYL
HHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HKSAGVMHMLGLADYQIPIESIDSSNIISAFEQTLAARGSIKTMMRSSIPALQSQLERLP
HHHCCHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHH
KQIRAAVGDWLRGAK
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]