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The map label for this gene is birA [H]

Identifier: 159899868

GI number: 159899868

Start: 4227190

End: 4228008

Strand: Direct

Name: birA [H]

Synonym: Haur_3351

Alternate gene names: 159899868

Gene position: 4227190-4228008 (Clockwise)

Preceding gene: 159899867

Following gene: 159899869

Centisome position: 66.61

GC content: 53.24

Gene sequence:

>819_bases
ATGCTGACATCATTTGAACAAACAATTATCGAACCAGCCCGTTATCAGCTAGAAACTTTGATTATTGGCCAAGAATTGCT
TGGACTCGAACAAACTGCCTCAACCAACAGCCTCATTCGTGAACGTGCTTTTGCAGGAGTCTCCGAGGGCTTGGTGATTG
TGGCCAACGAACAAACCGCTGGCCGTGGGCGACGCGGACGCACTTGGAGCGCTCCAGCTGGCAGCAGTTTGTTGCTTTCG
CTACTGTTACGCCCAACCTGGCTCTTGCCAAGCGAAAGCTTTTTATTGACCATGCTGGCGGCGGTGGCAATTAGCGAAGC
AATCGAACGCGAAACCAATTGCAAGGTCGAGCTAAAATGGCCCAACGATCTGTTAATTAATGGGCGGAAATTGGCGGGCA
TTTTGGTTGAGCTAGAAACGGCTGGCCCCGAACGGCTACGCTGGGCCGTGATTGGGAGCGGTATCAACGTCAATTGGCAG
CCAACCGAAGATCCAAGCATCAACCAAGCGGCTACATCGCTGGCCTTGGAATGTGGCAAGGATGTTGATCGGGCGGCCTT
GTTACGGGCAATTTTACAAAGTTACGATCGCCAGTATCTGAGCTTACGCAGTGGCCGCCGCGAAGCCTTACGCCAAGCCT
GGCTCAGCCGTTTAACCACGCTCGGCCAAACCGTCGAAGTTGAGTTGAGCAGCCACAATTTCACTGGCGTGGCCGAATCG
GTCGATCAGCATGGCGGGTTGCAAGTGCGCAGCAGCGATGGTCAACTACATACGGTTTACGCGGGCGATGTTAAAGTTCG
CCCAGTAGTACAAGCATAA

Upstream 100 bases:

>100_bases
AGTTTGCTGGGCCAATCATTGCGGTTTCGCATGATCGGCGCTTTATTCAGCAATTTGCTGGCGAGATTTGGCATTTACAA
CAGGGACGGTTAACGCGCCT

Downstream 100 bases:

>100_bases
TTTGGCAATCAAGGCAATAGCCTAGCGCAACTCAAGCCCCAAGGTTTGACAGCGTTTTAGGCTATTGTTATACTCGCCAC
GTTGTGACACCTGTCACATG

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MLTSFEQTIIEPARYQLETLIIGQELLGLEQTASTNSLIRERAFAGVSEGLVIVANEQTAGRGRRGRTWSAPAGSSLLLS
LLLRPTWLLPSESFLLTMLAAVAISEAIERETNCKVELKWPNDLLINGRKLAGILVELETAGPERLRWAVIGSGINVNWQ
PTEDPSINQAATSLALECGKDVDRAALLRAILQSYDRQYLSLRSGRREALRQAWLSRLTTLGQTVEVELSSHNFTGVAES
VDQHGGLQVRSSDGQLHTVYAGDVKVRPVVQA

Sequences:

>Translated_272_residues
MLTSFEQTIIEPARYQLETLIIGQELLGLEQTASTNSLIRERAFAGVSEGLVIVANEQTAGRGRRGRTWSAPAGSSLLLS
LLLRPTWLLPSESFLLTMLAAVAISEAIERETNCKVELKWPNDLLINGRKLAGILVELETAGPERLRWAVIGSGINVNWQ
PTEDPSINQAATSLALECGKDVDRAALLRAILQSYDRQYLSLRSGRREALRQAWLSRLTTLGQTVEVELSSHNFTGVAES
VDQHGGLQVRSSDGQLHTVYAGDVKVRPVVQA
>Mature_272_residues
MLTSFEQTIIEPARYQLETLIIGQELLGLEQTASTNSLIRERAFAGVSEGLVIVANEQTAGRGRRGRTWSAPAGSSLLLS
LLLRPTWLLPSESFLLTMLAAVAISEAIERETNCKVELKWPNDLLINGRKLAGILVELETAGPERLRWAVIGSGINVNWQ
PTEDPSINQAATSLALECGKDVDRAALLRAILQSYDRQYLSLRSGRREALRQAWLSRLTTLGQTVEVELSSHNFTGVAES
VDQHGGLQVRSSDGQLHTVYAGDVKVRPVVQA

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=168, Percent_Identity=33.9285714285714, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1790408, Length=244, Percent_Identity=31.5573770491803, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 29781; Mature: 29781

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSFEQTIIEPARYQLETLIIGQELLGLEQTASTNSLIRERAFAGVSEGLVIVANEQTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCCEEEEECCCCC
GRGRRGRTWSAPAGSSLLLSLLLRPTWLLPSESFLLTMLAAVAISEAIERETNCKVELKW
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PNDLLINGRKLAGILVELETAGPERLRWAVIGSGINVNWQPTEDPSINQAATSLALECGK
CCCEEECCCEEEEEEEEEECCCCCCEEEEEEECCEEECCCCCCCCCHHHHHHHHHHHCCC
DVDRAALLRAILQSYDRQYLSLRSGRREALRQAWLSRLTTLGQTVEVELSSHNFTGVAES
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
VDQHGGLQVRSSDGQLHTVYAGDVKVRPVVQA
HHHCCCEEEECCCCCEEEEEECCEEEEEECCC
>Mature Secondary Structure
MLTSFEQTIIEPARYQLETLIIGQELLGLEQTASTNSLIRERAFAGVSEGLVIVANEQTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCCEEEEECCCCC
GRGRRGRTWSAPAGSSLLLSLLLRPTWLLPSESFLLTMLAAVAISEAIERETNCKVELKW
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PNDLLINGRKLAGILVELETAGPERLRWAVIGSGINVNWQPTEDPSINQAATSLALECGK
CCCEEECCCEEEEEEEEEECCCCCCEEEEEEECCEEECCCCCCCCCHHHHHHHHHHHCCC
DVDRAALLRAILQSYDRQYLSLRSGRREALRQAWLSRLTTLGQTVEVELSSHNFTGVAES
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
VDQHGGLQVRSSDGQLHTVYAGDVKVRPVVQA
HHHCCCEEEECCCCCEEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]