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The map label for this gene is mscL

Identifier: 159899829

GI number: 159899829

Start: 4177113

End: 4177496

Strand: Direct

Name: mscL

Synonym: Haur_3312

Alternate gene names: 159899829

Gene position: 4177113-4177496 (Clockwise)

Preceding gene: 159899827

Following gene: 159899831

Centisome position: 65.82

GC content: 42.45

Gene sequence:

>384_bases
ATGTTTAAGGAGTTCAAGGAATTTGCCTTCAAAGGCAATGTTCTCGATTTGGCAATTGGGGTGATTATTGGGGCCGCTTT
TGGCAAAATTGTCACAGCACTGGTTGATGTTGTGATTATGCCGATTATCAGCATCATTCTTAGCCTCATTCTCAATGATG
TTAATATTGCAACATGGCAATTTTCGATTGGGGCTACACCAATTATGATTGGGGTACTGATCAAAACCATTATTGAGTTC
CTAATTATTGCGTTTGTGCTTTTCTTATTTGTCAAAGGCATCAACTCGACCCGCCGCAAGCAAGAAGTCGAAGCACCAGC
CGCGCCACCACCAAGCGAAGAAGTGTTGTTGCTACGCGAAATTCGCGATAGCTTACAAAAGTAA

Upstream 100 bases:

>100_bases
ATGTGGCTTAAATAATCGTTAATTTCACCGTAAAGCGTAGCTTTTAGACTACGCTTGTTTGCTCGATGGCCTAGAGTGGC
CTAGTACCGGAGGATACGCG

Downstream 100 bases:

>100_bases
AAGCCCTCACCCCAATCCCTTTCCTTACAAGGGTAGGTTTCTGCTACGAACCACGGATAGGCTGGTTCATCGATGCATGG
ATGCCAGCCCTGATTGCCAT

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MFKEFKEFAFKGNVLDLAIGVIIGAAFGKIVTALVDVVIMPIISIILSLILNDVNIATWQFSIGATPIMIGVLIKTIIEF
LIIAFVLFLFVKGINSTRRKQEVEAPAAPPPSEEVLLLREIRDSLQK

Sequences:

>Translated_127_residues
MFKEFKEFAFKGNVLDLAIGVIIGAAFGKIVTALVDVVIMPIISIILSLILNDVNIATWQFSIGATPIMIGVLIKTIIEF
LIIAFVLFLFVKGINSTRRKQEVEAPAAPPPSEEVLLLREIRDSLQK
>Mature_127_residues
MFKEFKEFAFKGNVLDLAIGVIIGAAFGKIVTALVDVVIMPIISIILSLILNDVNIATWQFSIGATPIMIGVLIKTIIEF
LIIAFVLFLFVKGINSTRRKQEVEAPAAPPPSEEVLLLREIRDSLQK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=128, Percent_Identity=48.4375, Blast_Score=107, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_HERA2 (A9B815)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001546076.1
- ProteinModelPortal:   A9B815
- SMR:   A9B815
- GeneID:   5735182
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_3312
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- BioCyc:   HAUR316274:HAUR_3312-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 13996; Mature: 13996

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17890c80)-; HASH(0x17d29e74)-; HASH(0x16b28d48)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEFKEFAFKGNVLDLAIGVIIGAAFGKIVTALVDVVIMPIISIILSLILNDVNIATWQ
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEE
FSIGATPIMIGVLIKTIIEFLIIAFVLFLFVKGINSTRRKQEVEAPAAPPPSEEVLLLRE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHH
IRDSLQK
HHHHHCH
>Mature Secondary Structure
MFKEFKEFAFKGNVLDLAIGVIIGAAFGKIVTALVDVVIMPIISIILSLILNDVNIATWQ
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEE
FSIGATPIMIGVLIKTIIEFLIIAFVLFLFVKGINSTRRKQEVEAPAAPPPSEEVLLLRE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHH
IRDSLQK
HHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA