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The map label for this gene is cobB [H]

Identifier: 159899689

GI number: 159899689

Start: 4008798

End: 4009547

Strand: Reverse

Name: cobB [H]

Synonym: Haur_3171

Alternate gene names: 159899689

Gene position: 4009547-4008798 (Counterclockwise)

Preceding gene: 159899690

Following gene: 159899686

Centisome position: 63.18

GC content: 50.0

Gene sequence:

>750_bases
ATGGCCAAGTTTCCCGAAGAGCTTCTACAACGCTTACGCCAAGCCGAGCGAATCGCAATTTTGACTGGAGCTGGAATCTC
GGCAGAAAGCGGGATTCCAACCTTTCGCGATGCCCAAACTGGCTTTTGGTCGCGCATGAAACCCGAAGATCTAGCAACGC
CCGAAGCATTCGAGCGCAATCCTAAGCTGGTTTGGGATTGGTATGCCTATCGGCGTGAAATGGTTGATCAAGCTAGCCCG
AATGCAGGCCATTATGCCTTGGCCAAGATGGCTGAACATGTGCCAAATTTGACCCTAATTACCCAAAATGTTGATAGTTT
GCATCAGCGGGCTGGCTCAACCAATGTTTTGGAGTTGCATGGCAACATTGGGCGAGTCAAATGCTCCAAAGAACATGTCG
TGATTGAACCATCGGCATGGTTGCCTGATGAAGAAATTGGTGTGCCCCGCTGCCCACGTTGCCAAGCCTTGTTGCGGCCT
GATATTGTTTGGTTTGGCGAAGTGTTACCAACCAAAACCTTTAATCATGCCAAAGCTGCCTGCATTCAAGCTGATGTGGT
TTTTTCGATTGGTACATCGGGTTTAGTGCCACCAGCCGCCACCTTGCCCGTCACCGCAATTGAACATGGAGCCTATGGCA
TCGAAGTTAATACTGACCAAACGTACCTCTCTGATACCTTTGAGTGCTGTTTACGCGGTGCAAGCGGCATTATTTTGCCT
GCCTTGGTCAAAGCAGCATGGCCTGATTGA

Upstream 100 bases:

>100_bases
GCTTTTTTACCGATCATGCCTTCCATGGCAAATTGGCCGAACTATTACCACAATTAGTTGCTGCGCTTGAGTAATTGACT
TGATTAGAGAGGAGTTGGTT

Downstream 100 bases:

>100_bases
AAATCAAAAGTCAGAAATCAGAAGGCAAAAGGGCATAGCGTTAACGCAGGGGAGCAGAGATAAAAGGATGAAGGATGAAC
AACAGTGCTTCGCGGTCGTC

Product: silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MAKFPEELLQRLRQAERIAILTGAGISAESGIPTFRDAQTGFWSRMKPEDLATPEAFERNPKLVWDWYAYRREMVDQASP
NAGHYALAKMAEHVPNLTLITQNVDSLHQRAGSTNVLELHGNIGRVKCSKEHVVIEPSAWLPDEEIGVPRCPRCQALLRP
DIVWFGEVLPTKTFNHAKAACIQADVVFSIGTSGLVPPAATLPVTAIEHGAYGIEVNTDQTYLSDTFECCLRGASGIILP
ALVKAAWPD

Sequences:

>Translated_249_residues
MAKFPEELLQRLRQAERIAILTGAGISAESGIPTFRDAQTGFWSRMKPEDLATPEAFERNPKLVWDWYAYRREMVDQASP
NAGHYALAKMAEHVPNLTLITQNVDSLHQRAGSTNVLELHGNIGRVKCSKEHVVIEPSAWLPDEEIGVPRCPRCQALLRP
DIVWFGEVLPTKTFNHAKAACIQADVVFSIGTSGLVPPAATLPVTAIEHGAYGIEVNTDQTYLSDTFECCLRGASGIILP
ALVKAAWPD
>Mature_248_residues
AKFPEELLQRLRQAERIAILTGAGISAESGIPTFRDAQTGFWSRMKPEDLATPEAFERNPKLVWDWYAYRREMVDQASPN
AGHYALAKMAEHVPNLTLITQNVDSLHQRAGSTNVLELHGNIGRVKCSKEHVVIEPSAWLPDEEIGVPRCPRCQALLRPD
IVWFGEVLPTKTFNHAKAACIQADVVFSIGTSGLVPPAATLPVTAIEHGAYGIEVNTDQTYLSDTFECCLRGASGIILPA
LVKAAWPD

Specific function: Modulates the activity of chromatin proteins, thereby having an effect on transcription per subunit [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912664, Length=251, Percent_Identity=37.4501992031873, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI300795542, Length=241, Percent_Identity=37.7593360995851, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI13787215, Length=238, Percent_Identity=37.8151260504202, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI6912660, Length=227, Percent_Identity=33.920704845815, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI13775602, Length=236, Percent_Identity=30.5084745762712, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI13775600, Length=236, Percent_Identity=30.5084745762712, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI300797705, Length=198, Percent_Identity=31.8181818181818, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI63054862, Length=215, Percent_Identity=33.953488372093, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI7657575, Length=228, Percent_Identity=30.7017543859649, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI6912662, Length=269, Percent_Identity=28.2527881040892, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI215982798, Length=173, Percent_Identity=29.4797687861272, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI308199517, Length=233, Percent_Identity=41.6309012875536, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71990482, Length=225, Percent_Identity=31.1111111111111, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71990487, Length=227, Percent_Identity=30.8370044052863, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17567771, Length=215, Percent_Identity=30.6976744186047, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17541892, Length=204, Percent_Identity=30.3921568627451, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6325242, Length=214, Percent_Identity=28.5046728971963, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648389, Length=206, Percent_Identity=30.5825242718447, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137536, Length=268, Percent_Identity=27.2388059701493, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571445, Length=201, Percent_Identity=30.3482587064677, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28571443, Length=134, Percent_Identity=30.5970149253731, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571441, Length=134, Percent_Identity=30.5970149253731, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27385; Mature: 27253

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFPEELLQRLRQAERIAILTGAGISAESGIPTFRDAQTGFWSRMKPEDLATPEAFERN
CCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCHHCCCCCHHCCCCHHHCCC
PKLVWDWYAYRREMVDQASPNAGHYALAKMAEHVPNLTLITQNVDSLHQRAGSTNVLELH
CCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEEC
GNIGRVKCSKEHVVIEPSAWLPDEEIGVPRCPRCQALLRPDIVWFGEVLPTKTFNHAKAA
CCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCEEHHHHCCCCCCCHHHHH
CIQADVVFSIGTSGLVPPAATLPVTAIEHGAYGIEVNTDQTYLSDTFECCLRGASGIILP
HHHHEEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHH
ALVKAAWPD
HHHHHCCCC
>Mature Secondary Structure 
AKFPEELLQRLRQAERIAILTGAGISAESGIPTFRDAQTGFWSRMKPEDLATPEAFERN
CCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCHHCCCCCHHCCCCHHHCCC
PKLVWDWYAYRREMVDQASPNAGHYALAKMAEHVPNLTLITQNVDSLHQRAGSTNVLELH
CCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEEC
GNIGRVKCSKEHVVIEPSAWLPDEEIGVPRCPRCQALLRPDIVWFGEVLPTKTFNHAKAA
CCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCEEHHHHCCCCCCCHHHHH
CIQADVVFSIGTSGLVPPAATLPVTAIEHGAYGIEVNTDQTYLSDTFECCLRGASGIILP
HHHHEEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHH
ALVKAAWPD
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]