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The map label for this gene is tilS [H]

Identifier: 159899546

GI number: 159899546

Start: 3822786

End: 3824246

Strand: Direct

Name: tilS [H]

Synonym: Haur_3027

Alternate gene names: 159899546

Gene position: 3822786-3824246 (Clockwise)

Preceding gene: 159899543

Following gene: 159899547

Centisome position: 60.23

GC content: 54.28

Gene sequence:

>1461_bases
ATGTCCCAACTACTCGACATTGAAACAACCCTCATCCGCGATGGTCGCCTCGCATCGGCAGCATGTTTGGTGGTGGCGGT
TTCAGGTGGCCCGGATTCACTAGCCTTGCTGCATCGGTTGACGGCCTTGGCCACACCTTATGGCTGGCGTTTGCATGTGG
CCCACCTTGATCATGGCTTGCGTGGCGAGCAATCAGCTGCCGAGGCCGCTTTTGTGGCCGAAATTGCCGCTGATTGGGGC
TTAACTGCCACAATTGAGCTGCGTGATGTTGACCAAGTTGCCAAACAATATCATGTGGGCTTGCCTGCTGCGGCCCGAGC
GACCCGCTATCGTTTTTTGGCTGAGGTGGCTTTGCAGCAACAAGCCGATGCGGTCGTACTCGGCCATCAATCCGATGATC
AAGCAGAAACGGTGTTGTTGCATGTGTTGCGGGGCACAGGCCCGAGCGGCTTGAGCGCGATGCGACCACGCACCGAGTGG
GTTGGCTGGCGAGTTACCGCAGGAATGCCCCATCAAGCAGTTGGGCCAGCTTTGATTCGTCCGATGCTCAATATTACCCG
CGACGAAATTGAGGCCTATTGCGATGAACAGCAATTGGAGCCACGCCGCGACCCAACCAATAATTCATTGGAATATACGC
GGGGCCGCATTCGCCATCAATTATTGCCATTGCTCGGCGAATACAATGCGAAAGTTGTTTCGGCCTTGGCGCGAACGGCC
CGTTTAGCGGCGATTGAAACCGACTACATGCAAAGCGAGCTTGATCGTCAATGGCAACATTTAATTGAGTTTGAGCCAGG
GGCGATTACCTTTCATCGTGAGCAATGGAACCACATGCATCCAGCGTTGCAGCGCCATGCAATTCGCCGCGCGGTTTATT
TATTGCGGCCTGAGCGCGACGAATTAACATCCTCGCAGCTTGACCATGCGTTAAGTGCCATGAACAGCCTGCAACTGTTT
TGCCAAATGCCGCATAATTTGGTCTTGCATCGCAAGCCACATGGTTGGACAATGACCGTAGCTACGACTATGCAGCCAAC
CAACGACGAACCGCAATTGAGCATCGATGAGTGGCCGCTCAATCAACAAGGCATGACTCCGTTGCCCGATGAACGTTGGC
GGGTGATTATGGAGCTGATGCCCAGCCGCCCTGAATATAATCTTTCGCGCTGGCAAGGCTTGCTCGATGCCAACGCAATT
CAAGGCAGATTGATGTTACGTCAACGCCGCCCAGGCGATCGCATGCGGCCCGCAGGTGGCATTGGCTCACGGCGCATCCA
AGATATTATGGTTGATATGCGTTTGCCACGCGAACTGCGTGCCCAATGGCCGTTATTCGTTGATGATGTTGGGATACTTT
GGATTCCTGGCTTGCTGATGGCCGAACATGCCCACCCAACGCCAGAAACAACCAACTTTTTGCTCATAACTTTGGAGGAT
ACTCACGCCGATGCATCCTGA

Upstream 100 bases:

>100_bases
TCCTAGCCACCCCAAGCCGCAAACTCCATCATGAGATTGATATGGCTAGCCGCAAAAATGTGCTATCATCAAGGTTGGCA
CCACTTACGAGAACTCTGCT

Downstream 100 bases:

>100_bases
TCTTGAGCGAGTATTAATTGATACCGCAACACTCCAAAACCGAGTTCAAGCGCTTGGCACAGCAATTGCCCAAGCTACGG
TTAACGCACCTGAGTTAGTG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSQLLDIETTLIRDGRLASAACLVVAVSGGPDSLALLHRLTALATPYGWRLHVAHLDHGLRGEQSAAEAAFVAEIAADWG
LTATIELRDVDQVAKQYHVGLPAAARATRYRFLAEVALQQQADAVVLGHQSDDQAETVLLHVLRGTGPSGLSAMRPRTEW
VGWRVTAGMPHQAVGPALIRPMLNITRDEIEAYCDEQQLEPRRDPTNNSLEYTRGRIRHQLLPLLGEYNAKVVSALARTA
RLAAIETDYMQSELDRQWQHLIEFEPGAITFHREQWNHMHPALQRHAIRRAVYLLRPERDELTSSQLDHALSAMNSLQLF
CQMPHNLVLHRKPHGWTMTVATTMQPTNDEPQLSIDEWPLNQQGMTPLPDERWRVIMELMPSRPEYNLSRWQGLLDANAI
QGRLMLRQRRPGDRMRPAGGIGSRRIQDIMVDMRLPRELRAQWPLFVDDVGILWIPGLLMAEHAHPTPETTNFLLITLED
THADAS

Sequences:

>Translated_486_residues
MSQLLDIETTLIRDGRLASAACLVVAVSGGPDSLALLHRLTALATPYGWRLHVAHLDHGLRGEQSAAEAAFVAEIAADWG
LTATIELRDVDQVAKQYHVGLPAAARATRYRFLAEVALQQQADAVVLGHQSDDQAETVLLHVLRGTGPSGLSAMRPRTEW
VGWRVTAGMPHQAVGPALIRPMLNITRDEIEAYCDEQQLEPRRDPTNNSLEYTRGRIRHQLLPLLGEYNAKVVSALARTA
RLAAIETDYMQSELDRQWQHLIEFEPGAITFHREQWNHMHPALQRHAIRRAVYLLRPERDELTSSQLDHALSAMNSLQLF
CQMPHNLVLHRKPHGWTMTVATTMQPTNDEPQLSIDEWPLNQQGMTPLPDERWRVIMELMPSRPEYNLSRWQGLLDANAI
QGRLMLRQRRPGDRMRPAGGIGSRRIQDIMVDMRLPRELRAQWPLFVDDVGILWIPGLLMAEHAHPTPETTNFLLITLED
THADAS
>Mature_485_residues
SQLLDIETTLIRDGRLASAACLVVAVSGGPDSLALLHRLTALATPYGWRLHVAHLDHGLRGEQSAAEAAFVAEIAADWGL
TATIELRDVDQVAKQYHVGLPAAARATRYRFLAEVALQQQADAVVLGHQSDDQAETVLLHVLRGTGPSGLSAMRPRTEWV
GWRVTAGMPHQAVGPALIRPMLNITRDEIEAYCDEQQLEPRRDPTNNSLEYTRGRIRHQLLPLLGEYNAKVVSALARTAR
LAAIETDYMQSELDRQWQHLIEFEPGAITFHREQWNHMHPALQRHAIRRAVYLLRPERDELTSSQLDHALSAMNSLQLFC
QMPHNLVLHRKPHGWTMTVATTMQPTNDEPQLSIDEWPLNQQGMTPLPDERWRVIMELMPSRPEYNLSRWQGLLDANAIQ
GRLMLRQRRPGDRMRPAGGIGSRRIQDIMVDMRLPRELRAQWPLFVDDVGILWIPGLLMAEHAHPTPETTNFLLITLEDT
HADAS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=236, Percent_Identity=32.2033898305085, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54890; Mature: 54758

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLLDIETTLIRDGRLASAACLVVAVSGGPDSLALLHRLTALATPYGWRLHVAHLDHGL
CCCCCCCHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCC
RGEQSAAEAAFVAEIAADWGLTATIELRDVDQVAKQYHVGLPAAARATRYRFLAEVALQQ
CCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QADAVVLGHQSDDQAETVLLHVLRGTGPSGLSAMRPRTEWVGWRVTAGMPHQAVGPALIR
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHCCEEEECCCCCHHCCHHHHH
PMLNITRDEIEAYCDEQQLEPRRDPTNNSLEYTRGRIRHQLLPLLGEYNAKVVSALARTA
HHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RLAAIETDYMQSELDRQWQHLIEFEPGAITFHREQWNHMHPALQRHAIRRAVYLLRPERD
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ELTSSQLDHALSAMNSLQLFCQMPHNLVLHRKPHGWTMTVATTMQPTNDEPQLSIDEWPL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCEEECCCCC
NQQGMTPLPDERWRVIMELMPSRPEYNLSRWQGLLDANAIQGRLMLRQRRPGDRMRPAGG
CCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCC
IGSRRIQDIMVDMRLPRELRAQWPLFVDDVGILWIPGLLMAEHAHPTPETTNFLLITLED
CCHHHHHHHHHHHCCCHHHHCCCCEEEECCCHHHHCHHHHHHCCCCCCCCCCEEEEEEEC
THADAS
CCCCCC
>Mature Secondary Structure 
SQLLDIETTLIRDGRLASAACLVVAVSGGPDSLALLHRLTALATPYGWRLHVAHLDHGL
CCCCCCHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCC
RGEQSAAEAAFVAEIAADWGLTATIELRDVDQVAKQYHVGLPAAARATRYRFLAEVALQQ
CCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QADAVVLGHQSDDQAETVLLHVLRGTGPSGLSAMRPRTEWVGWRVTAGMPHQAVGPALIR
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHCCEEEECCCCCHHCCHHHHH
PMLNITRDEIEAYCDEQQLEPRRDPTNNSLEYTRGRIRHQLLPLLGEYNAKVVSALARTA
HHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RLAAIETDYMQSELDRQWQHLIEFEPGAITFHREQWNHMHPALQRHAIRRAVYLLRPERD
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ELTSSQLDHALSAMNSLQLFCQMPHNLVLHRKPHGWTMTVATTMQPTNDEPQLSIDEWPL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCEEECCCCC
NQQGMTPLPDERWRVIMELMPSRPEYNLSRWQGLLDANAIQGRLMLRQRRPGDRMRPAGG
CCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCC
IGSRRIQDIMVDMRLPRELRAQWPLFVDDVGILWIPGLLMAEHAHPTPETTNFLLITLED
CCHHHHHHHHHHHCCCHHHHCCCCEEEECCCHHHHCHHHHHHCCCCCCCCCCEEEEEEEC
THADAS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]