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The map label for this gene is rlmI [H]

Identifier: 159899517

GI number: 159899517

Start: 3786122

End: 3787315

Strand: Direct

Name: rlmI [H]

Synonym: Haur_2998

Alternate gene names: 159899517

Gene position: 3786122-3787315 (Clockwise)

Preceding gene: 159899516

Following gene: 159899518

Centisome position: 59.66

GC content: 53.43

Gene sequence:

>1194_bases
GTGGCCGCCCGTTCAATGCCAACAATCAACTTGCCAACCCAGCTAAAGGCTCATCTTCAGGCCGGCCATCCATGGGTCTA
CCGCGACCATGTGCCGCCGAGTACTCGTTTAGCCAGCGGCACATGGGTGCGGCTGCATTGTGGCAATTGGCAAGGGTTTG
GCTTGTGGGATGCTCGCTCGCCGATCGCTCTACGCATCTTTTCGAGCCGCATGCAGCCCGATGCCAACTGGATCAAAACG
GTCGTTCAGCAAGCATGGCAGGCACGTGAACCGTTGCGCCAAACTGCCACCACCGCCTATCGTTTGCTGTTTGGCGAGGG
CGATGGCCTACCAGGGATCACCATCGATCTCTACAATCAATATGCCGTGATTGCGACCTACGCCGATTGTGTTGAGGTAT
TGATTGCCGATGTGGTCAAGGCCTTGCAAGCCAGCGTGCCACAACTGCGGGGCGTGGTGCGCCGCCGCCGCGACGATAGC
GAAAACGACGATGAAACTGGCAAAATTGAGTTGTTGTGGGGCGAATTGCCACCAGCCCAACTGATAGTCGAAGAACATGG
CCTTAAATTGATCGCTAATTTGTTTGAGGGCCAGAAAACTGGCTTATTCCTTGATCATCGTGAGAACCGCCATACCATTG
AGCAATGGAGCCATGGTAAAACGGTGCTGAATTGCTTCTCGTATACTGGGGCATTTTCGTTATACGCTGCTCGTGGCGGC
GCAACTGCCACCACCAGCGTCGATATTGCGCCAGCCGCTGCCCACGATGCTGAACAAAATTTTATGCTCAATGGCTTGAT
GAATGAACACCAGCGCTTTTTGGCCCGCGATTGCTTTGATTTTCTGAGTCGCACGATTCAGCGTGGCGAAACCTATGATT
TGGTGATTCTTGACCCACCTTCGTTTGCCCGCTCGAAGAAAAATATTCATGCAGCAACTCGAGCTTATGTCAAACTCAAT
GCCTTAGCGATTCAATGTGTGGCGAAGGGTGGGCTACTGGCCTCAGCCAGTTGTACTAGCCAACTTTCGCCCGCCAATTT
TCGCTTGATGCTGGGCGAAGCTGCTGCCCAAACCGATCAGCAATTGCGCATTATTCATGAGGCAGGGCAAGCGCTCGATC
ACCCAGTGCCAGCGCATTTTACCGAAGGCCGCTATCTCAAATTTGTGTTAGCCCGCGTTGATGAGCGTATGTAA

Upstream 100 bases:

>100_bases
GTGCTGGCTATACCAATTATCTTTCTAAGCCGTTTGATGTTCAAGATTTATTGCAGATGGTGCGCGGATACGTGCCATCG
GCAGTCTAGAGGAGTCGTGT

Downstream 100 bases:

>100_bases
AGTAATTCACCACCCTTCCGTCCCGCCTCAAGGCGGGAGACGCAGGGGGTTTTCATCCCCCTGCACCCCCAAATGTTTAT
GTTGTGGATTATTTCTGTTG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAARSMPTINLPTQLKAHLQAGHPWVYRDHVPPSTRLASGTWVRLHCGNWQGFGLWDARSPIALRIFSSRMQPDANWIKT
VVQQAWQAREPLRQTATTAYRLLFGEGDGLPGITIDLYNQYAVIATYADCVEVLIADVVKALQASVPQLRGVVRRRRDDS
ENDDETGKIELLWGELPPAQLIVEEHGLKLIANLFEGQKTGLFLDHRENRHTIEQWSHGKTVLNCFSYTGAFSLYAARGG
ATATTSVDIAPAAAHDAEQNFMLNGLMNEHQRFLARDCFDFLSRTIQRGETYDLVILDPPSFARSKKNIHAATRAYVKLN
ALAIQCVAKGGLLASASCTSQLSPANFRLMLGEAAAQTDQQLRIIHEAGQALDHPVPAHFTEGRYLKFVLARVDERM

Sequences:

>Translated_397_residues
MAARSMPTINLPTQLKAHLQAGHPWVYRDHVPPSTRLASGTWVRLHCGNWQGFGLWDARSPIALRIFSSRMQPDANWIKT
VVQQAWQAREPLRQTATTAYRLLFGEGDGLPGITIDLYNQYAVIATYADCVEVLIADVVKALQASVPQLRGVVRRRRDDS
ENDDETGKIELLWGELPPAQLIVEEHGLKLIANLFEGQKTGLFLDHRENRHTIEQWSHGKTVLNCFSYTGAFSLYAARGG
ATATTSVDIAPAAAHDAEQNFMLNGLMNEHQRFLARDCFDFLSRTIQRGETYDLVILDPPSFARSKKNIHAATRAYVKLN
ALAIQCVAKGGLLASASCTSQLSPANFRLMLGEAAAQTDQQLRIIHEAGQALDHPVPAHFTEGRYLKFVLARVDERM
>Mature_396_residues
AARSMPTINLPTQLKAHLQAGHPWVYRDHVPPSTRLASGTWVRLHCGNWQGFGLWDARSPIALRIFSSRMQPDANWIKTV
VQQAWQAREPLRQTATTAYRLLFGEGDGLPGITIDLYNQYAVIATYADCVEVLIADVVKALQASVPQLRGVVRRRRDDSE
NDDETGKIELLWGELPPAQLIVEEHGLKLIANLFEGQKTGLFLDHRENRHTIEQWSHGKTVLNCFSYTGAFSLYAARGGA
TATTSVDIAPAAAHDAEQNFMLNGLMNEHQRFLARDCFDFLSRTIQRGETYDLVILDPPSFARSKKNIHAATRAYVKLNA
LAIQCVAKGGLLASASCTSQLSPANFRLMLGEAAAQTDQQLRIIHEAGQALDHPVPAHFTEGRYLKFVLARVDERM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=379, Percent_Identity=35.8839050131926, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1787181, Length=228, Percent_Identity=32.8947368421053, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44208; Mature: 44077

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARSMPTINLPTQLKAHLQAGHPWVYRDHVPPSTRLASGTWVRLHCGNWQGFGLWDARS
CCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCEECCCCEEEEECCCCCCCEECCCCC
PIALRIFSSRMQPDANWIKTVVQQAWQAREPLRQTATTAYRLLFGEGDGLPGITIDLYNQ
CEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCC
YAVIATYADCVEVLIADVVKALQASVPQLRGVVRRRRDDSENDDETGKIELLWGELPPAQ
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHH
LIVEEHGLKLIANLFEGQKTGLFLDHRENRHTIEQWSHGKTVLNCFSYTGAFSLYAARGG
HHHHHCCHHHHHHHHCCCCCCEEEECCCCCCHHHHHCCCHHHHHHHHHCCCEEEEEECCC
ATATTSVDIAPAAAHDAEQNFMLNGLMNEHQRFLARDCFDFLSRTIQRGETYDLVILDPP
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
SFARSKKNIHAATRAYVKLNALAIQCVAKGGLLASASCTSQLSPANFRLMLGEAAAQTDQ
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCCCCCCCEEEEECCHHHCCHH
QLRIIHEAGQALDHPVPAHFTEGRYLKFVLARVDERM
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AARSMPTINLPTQLKAHLQAGHPWVYRDHVPPSTRLASGTWVRLHCGNWQGFGLWDARS
CCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCEECCCCEEEEECCCCCCCEECCCCC
PIALRIFSSRMQPDANWIKTVVQQAWQAREPLRQTATTAYRLLFGEGDGLPGITIDLYNQ
CEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCC
YAVIATYADCVEVLIADVVKALQASVPQLRGVVRRRRDDSENDDETGKIELLWGELPPAQ
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHH
LIVEEHGLKLIANLFEGQKTGLFLDHRENRHTIEQWSHGKTVLNCFSYTGAFSLYAARGG
HHHHHCCHHHHHHHHCCCCCCEEEECCCCCCHHHHHCCCHHHHHHHHHCCCEEEEEECCC
ATATTSVDIAPAAAHDAEQNFMLNGLMNEHQRFLARDCFDFLSRTIQRGETYDLVILDPP
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
SFARSKKNIHAATRAYVKLNALAIQCVAKGGLLASASCTSQLSPANFRLMLGEAAAQTDQ
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCCCCCCCEEEEECCHHHCCHH
QLRIIHEAGQALDHPVPAHFTEGRYLKFVLARVDERM
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA