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The map label for this gene is nfi [H]

Identifier: 159899443

GI number: 159899443

Start: 3696442

End: 3697140

Strand: Direct

Name: nfi [H]

Synonym: Haur_2924

Alternate gene names: 159899443

Gene position: 3696442-3697140 (Clockwise)

Preceding gene: 159899442

Following gene: 159899445

Centisome position: 58.24

GC content: 50.93

Gene sequence:

>699_bases
ATGAGTCGTTTTCCAAATAGCCAAGTGCATCGCTGGGATTTAACGCCCGAGCAAGCGATCGATCTGCAACTCCAACTACG
CGATCAGGTGCGTTTAGTCGATGATTTTGCCCAGCCACTTCAAACGGTGGCTGGGGTTGATGCTGCCTTTGAAGACGATG
GCGCAACCACCCGTGCCGCAATCGTGATCTTGAATTTTCCAGCACTTCAACCAGTCGAAAAAACCTTAGTTCGTCGTCCA
ACCAACTTCCCCTATATTCCTGGCTTGCTGTCATTTCGCGAAATTCCGGCAGTGTTGGCAGCCTTGGAGCAATTGCAACA
GCTGCCCGATGTGTTGTTGTGTGATGGGATGGGCATTATGCATCCCCGTCGTTTTGGCATTGCCGCTCATTTGGGTGTTT
TGACCGATTTACCAACAATTGGCGTGGGCAAAAGTTATTTATGCGGAACCCACGAACCAGTGCCCAATCAGCAAGGTGCG
TGGGTTCCCGTCTATGATGCTGGCGAACAGATTGGGGCGGTTGTGCGAACGCGGGTTGGCACAAATCCGCTCTACATTTC
GCCTGGCCATCGCGTAAGCATTAGCACTGCTGTCGATTTAGTGTTGGGTTGCACAACCAAATATCGATTGCCTGAAACTA
CCCGCCACGCCGACCAACTTTCCAAAGATAAAGCCAATAATGGTGGCATTCAACTCTGA

Upstream 100 bases:

>100_bases
TGTTGGCAAAACATCTCGATTATGACGAATTATTGATCGATGTACTAACCGATCGGGTTGCCGAACACCGACCTGTTATC
ACTAATGGATGAGGCAATAG

Downstream 100 bases:

>100_bases
GTAGAAGCAATCACACAACTGCTTGCACCTCGACTGCTATTCCATTTAAGGCAGCATTGCCACTTAATGGATCGATCGCT
TGATCATCGGTTAAATCGTT

Product: deoxyribonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSRFPNSQVHRWDLTPEQAIDLQLQLRDQVRLVDDFAQPLQTVAGVDAAFEDDGATTRAAIVILNFPALQPVEKTLVRRP
TNFPYIPGLLSFREIPAVLAALEQLQQLPDVLLCDGMGIMHPRRFGIAAHLGVLTDLPTIGVGKSYLCGTHEPVPNQQGA
WVPVYDAGEQIGAVVRTRVGTNPLYISPGHRVSISTAVDLVLGCTTKYRLPETTRHADQLSKDKANNGGIQL

Sequences:

>Translated_232_residues
MSRFPNSQVHRWDLTPEQAIDLQLQLRDQVRLVDDFAQPLQTVAGVDAAFEDDGATTRAAIVILNFPALQPVEKTLVRRP
TNFPYIPGLLSFREIPAVLAALEQLQQLPDVLLCDGMGIMHPRRFGIAAHLGVLTDLPTIGVGKSYLCGTHEPVPNQQGA
WVPVYDAGEQIGAVVRTRVGTNPLYISPGHRVSISTAVDLVLGCTTKYRLPETTRHADQLSKDKANNGGIQL
>Mature_231_residues
SRFPNSQVHRWDLTPEQAIDLQLQLRDQVRLVDDFAQPLQTVAGVDAAFEDDGATTRAAIVILNFPALQPVEKTLVRRPT
NFPYIPGLLSFREIPAVLAALEQLQQLPDVLLCDGMGIMHPRRFGIAAHLGVLTDLPTIGVGKSYLCGTHEPVPNQQGAW
VPVYDAGEQIGAVVRTRVGTNPLYISPGHRVSISTAVDLVLGCTTKYRLPETTRHADQLSKDKANNGGIQL

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=202, Percent_Identity=39.1089108910891, Blast_Score=127, Evalue=6e-30,
Organism=Homo sapiens, GI257467548, Length=197, Percent_Identity=33.502538071066, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI257467550, Length=197, Percent_Identity=33.502538071066, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI87082357, Length=207, Percent_Identity=48.3091787439614, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 25360; Mature: 25229

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFPNSQVHRWDLTPEQAIDLQLQLRDQVRLVDDFAQPLQTVAGVDAAFEDDGATTRAA
CCCCCCCCCEEECCCHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
IVILNFPALQPVEKTLVRRPTNFPYIPGLLSFREIPAVLAALEQLQQLPDVLLCDGMGIM
EEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
HPRRFGIAAHLGVLTDLPTIGVGKSYLCGTHEPVPNQQGAWVPVYDAGEQIGAVVRTRVG
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCC
TNPLYISPGHRVSISTAVDLVLGCTTKYRLPETTRHADQLSKDKANNGGIQL
CCCEEECCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SRFPNSQVHRWDLTPEQAIDLQLQLRDQVRLVDDFAQPLQTVAGVDAAFEDDGATTRAA
CCCCCCCCEEECCCHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
IVILNFPALQPVEKTLVRRPTNFPYIPGLLSFREIPAVLAALEQLQQLPDVLLCDGMGIM
EEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
HPRRFGIAAHLGVLTDLPTIGVGKSYLCGTHEPVPNQQGAWVPVYDAGEQIGAVVRTRVG
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCC
TNPLYISPGHRVSISTAVDLVLGCTTKYRLPETTRHADQLSKDKANNGGIQL
CCCEEECCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA