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The map label for this gene is gcvH

Identifier: 159899388

GI number: 159899388

Start: 3640443

End: 3640832

Strand: Direct

Name: gcvH

Synonym: Haur_2869

Alternate gene names: 159899388

Gene position: 3640443-3640832 (Clockwise)

Preceding gene: 159899387

Following gene: 159899389

Centisome position: 57.36

GC content: 48.46

Gene sequence:

>390_bases
ATGTCCAATGTACCACATGAATTGCTGTATACCAAAGAACACGAATGGGTGCGCATCGACGGCAACAGTGCAGTTGTTGG
CATCACCGAACACGCCCAACGCGAGCTTGGCGATGTAGTATTCGTTGAATTGCCTGATGTTGGCAAAAGCTTTGAAGTTG
GCGAGCCATTTGGAACGGTCGAATCAGTCAAGGCAGTTTCGGAAATTTATGCTCCGCTCAGTGGTGAGATTACCGAAATC
AACAACGAAGTCGTTGATTCACCAGAATTGGTCAATGAAGACCCCTATGGCGAGGGCTGGTTGGCCAAATTCAGCTTTAC
CACTGCACCAAGTGGGTTGTTGAGTGCCGCAGAGTACGAACGCTATATCAACGACGAAGCCAATAAGTAG

Upstream 100 bases:

>100_bases
CCTGCTGCTCTTGCATAAAAAGCCGCTTTGCTTGATAGTAGCGCCTAACAATCTATGCTGATTGTGTGCTGTTTTGGCGT
TATTGAAGGAGGACCACCGA

Downstream 100 bases:

>100_bases
CAATTCAGAAGTCAAAAATCAAAAGTCAAAATAGCCAGACCCACAAGTTATGCTATTTGACTAAAGTATTTAACGCTGAG
AAATCAAAAGGTGATTGGAA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSNVPHELLYTKEHEWVRIDGNSAVVGITEHAQRELGDVVFVELPDVGKSFEVGEPFGTVESVKAVSEIYAPLSGEITEI
NNEVVDSPELVNEDPYGEGWLAKFSFTTAPSGLLSAAEYERYINDEANK

Sequences:

>Translated_129_residues
MSNVPHELLYTKEHEWVRIDGNSAVVGITEHAQRELGDVVFVELPDVGKSFEVGEPFGTVESVKAVSEIYAPLSGEITEI
NNEVVDSPELVNEDPYGEGWLAKFSFTTAPSGLLSAAEYERYINDEANK
>Mature_128_residues
SNVPHELLYTKEHEWVRIDGNSAVVGITEHAQRELGDVVFVELPDVGKSFEVGEPFGTVESVKAVSEIYAPLSGEITEIN
NEVVDSPELVNEDPYGEGWLAKFSFTTAPSGLLSAAEYERYINDEANK

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=46.218487394958, Blast_Score=108, Evalue=1e-24,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=45.3781512605042, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=51.937984496124, Blast_Score=131, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=45, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=44.1666666666667, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=42.1875, Blast_Score=108, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17865652, Length=130, Percent_Identity=40, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_HERA2 (A9B2Q6)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001545635.1
- ProteinModelPortal:   A9B2Q6
- SMR:   A9B2Q6
- GeneID:   5734740
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_2869
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   HAUR316274:HAUR_2869-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14217; Mature: 14086

Theoretical pI: Translated: 3.97; Mature: 3.97

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPHELLYTKEHEWVRIDGNSAVVGITEHAQRELGDVVFVELPDVGKSFEVGEPFGTV
CCCCCCHHEEECCCCEEEECCCCEEEEEHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHH
ESVKAVSEIYAPLSGEITEINNEVVDSPELVNEDPYGEGWLAKFSFTTAPSGLLSAAEYE
HHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
RYINDEANK
HHHCCCCCC
>Mature Secondary Structure 
SNVPHELLYTKEHEWVRIDGNSAVVGITEHAQRELGDVVFVELPDVGKSFEVGEPFGTV
CCCCCHHEEECCCCEEEECCCCEEEEEHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHH
ESVKAVSEIYAPLSGEITEINNEVVDSPELVNEDPYGEGWLAKFSFTTAPSGLLSAAEYE
HHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
RYINDEANK
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA