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The map label for this gene is prfA [H]

Identifier: 159899315

GI number: 159899315

Start: 3555041

End: 3556138

Strand: Direct

Name: prfA [H]

Synonym: Haur_2796

Alternate gene names: 159899315

Gene position: 3555041-3556138 (Clockwise)

Preceding gene: 159899313

Following gene: 159899316

Centisome position: 56.02

GC content: 52.82

Gene sequence:

>1098_bases
ATGGAACCAGAAATTTTAGCTCGTCTCGCGCGGGTCGAGGCGCGATATATCGAACTCAATGATGAAATGACTCAGCCTGA
TGCGCTGAGCGACCATGTGCGTTTAACTACATTGGCCCGCGAACAAACCGAACTTGGCGAATTAGTCGGGCGGTATCGCG
AGTTTATGCAGGCTGAAAAATCGGTCGAAGAAGCCAAAGCCCTTCTGCGCGAGGAGAATGATCCCGAGTTGCGCGAAATG
GCTCAATTGGAGATCGATGAGCTTTCGGCTCGCATGGAAGCAATCGGGGCTGAAGTACGGGTTTTGCTCTTGCCACGTGA
CCCCAACGATGAAAAGAACGTGATCATCGAAATTCGCCAAGGCGAAGGTGGCGATGAAGCAGCACTCTTCGGCGCTGATG
TCTATCGTATGTACCAACGTTATGCCGAAATTCGTGGTTGGAAAACCGAGGTTATGTCGCTCAGCGAAAATGGTATCGGC
GGCTTCAAAGAAGTTATTTTTGAAGTGCAAGGCCAAGGCGCTTGGAGCCGGCTTAAATATGAGGGCGGCGTACATCGCGT
TCAGCGCGTACCAGCCACCGAGGCCCGTGGCCGAATTCATACCAGCACGATCACGGTGGCGGTGTTACCAGAAGTCGAAG
AAACTGCCGTCGAAATCAAGCAAGAAGATTATCGCATCGACGTGTATCGCTCAGGTGGTCACGGCGGCCAAGGGGTCAAC
ACCACCGACTCAGCCGTGCGGATCGTGTACAAGGCTGGTACGCCTGAAGAAATTGTGGTTGTTTGTCAAGACACCCGTTC
GCAAATCAAAAATCGCGAAAGTGCCTTGAATGTGTTGCGGGCACGGCTCTATGCGCGTGAGCAAGAAAAACGCCAAAAAG
AGCTTGGGGAATCGCGCTTGGCTCAGGTTGGAAGCGGTGAGCGAGCTGAAAAAATGCGCACCTACAACTATCCTCAAGAC
CGCATTACCGATCATCGGATTGGCCAAAACATCTCAAACCTGCCGGTTGTGCTTGATGGAGCGCTTGATCGCTTGATCGA
TGCGCTAGTTACCTTCGACAACGCCGAACGGCTTAAGGCCTTGGGTATGGGAGCCTAG

Upstream 100 bases:

>100_bases
CTGTTCTATGTTCTATGTTCTTATGCTCTATGCCCTTTGTAAAAGCCTTATGCTATAATGGCGCAGATACACCACCAAGA
TAAGGAACATCCATACAACT

Downstream 100 bases:

>100_bases
GAGTTTGTTGCATGCACTCGCCGCTTGCTGAATTTATTCGCCGCATGCCCAAATGTGAGCTTCATGTTCACTTAGAAGGC
TCGATTACCCCTAAAACGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MEPEILARLARVEARYIELNDEMTQPDALSDHVRLTTLAREQTELGELVGRYREFMQAEKSVEEAKALLREENDPELREM
AQLEIDELSARMEAIGAEVRVLLLPRDPNDEKNVIIEIRQGEGGDEAALFGADVYRMYQRYAEIRGWKTEVMSLSENGIG
GFKEVIFEVQGQGAWSRLKYEGGVHRVQRVPATEARGRIHTSTITVAVLPEVEETAVEIKQEDYRIDVYRSGGHGGQGVN
TTDSAVRIVYKAGTPEEIVVVCQDTRSQIKNRESALNVLRARLYAREQEKRQKELGESRLAQVGSGERAEKMRTYNYPQD
RITDHRIGQNISNLPVVLDGALDRLIDALVTFDNAERLKALGMGA

Sequences:

>Translated_365_residues
MEPEILARLARVEARYIELNDEMTQPDALSDHVRLTTLAREQTELGELVGRYREFMQAEKSVEEAKALLREENDPELREM
AQLEIDELSARMEAIGAEVRVLLLPRDPNDEKNVIIEIRQGEGGDEAALFGADVYRMYQRYAEIRGWKTEVMSLSENGIG
GFKEVIFEVQGQGAWSRLKYEGGVHRVQRVPATEARGRIHTSTITVAVLPEVEETAVEIKQEDYRIDVYRSGGHGGQGVN
TTDSAVRIVYKAGTPEEIVVVCQDTRSQIKNRESALNVLRARLYAREQEKRQKELGESRLAQVGSGERAEKMRTYNYPQD
RITDHRIGQNISNLPVVLDGALDRLIDALVTFDNAERLKALGMGA
>Mature_365_residues
MEPEILARLARVEARYIELNDEMTQPDALSDHVRLTTLAREQTELGELVGRYREFMQAEKSVEEAKALLREENDPELREM
AQLEIDELSARMEAIGAEVRVLLLPRDPNDEKNVIIEIRQGEGGDEAALFGADVYRMYQRYAEIRGWKTEVMSLSENGIG
GFKEVIFEVQGQGAWSRLKYEGGVHRVQRVPATEARGRIHTSTITVAVLPEVEETAVEIKQEDYRIDVYRSGGHGGQGVN
TTDSAVRIVYKAGTPEEIVVVCQDTRSQIKNRESALNVLRARLYAREQEKRQKELGESRLAQVGSGERAEKMRTYNYPQD
RITDHRIGQNISNLPVVLDGALDRLIDALVTFDNAERLKALGMGA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=314, Percent_Identity=41.0828025477707, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI34577120, Length=324, Percent_Identity=37.962962962963, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI166795305, Length=204, Percent_Identity=41.6666666666667, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1787462, Length=362, Percent_Identity=46.6850828729282, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI2367172, Length=337, Percent_Identity=35.9050445103858, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=273, Percent_Identity=35.5311355311355, Blast_Score=171, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6321295, Length=320, Percent_Identity=42.1875, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI19921226, Length=309, Percent_Identity=42.7184466019417, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41182; Mature: 41182

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPEILARLARVEARYIELNDEMTQPDALSDHVRLTTLAREQTELGELVGRYREFMQAEK
CCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
SVEEAKALLREENDPELREMAQLEIDELSARMEAIGAEVRVLLLPRDPNDEKNVIIEIRQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEC
GEGGDEAALFGADVYRMYQRYAEIRGWKTEVMSLSENGIGGFKEVIFEVQGQGAWSRLKY
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHH
EGGVHRVQRVPATEARGRIHTSTITVAVLPEVEETAVEIKQEDYRIDVYRSGGHGGQGVN
CCCHHHHHHCCCHHHCCEEEEEEEEEEECCCHHHHHHHHHHCCEEEEEEECCCCCCCCCC
TTDSAVRIVYKAGTPEEIVVVCQDTRSQIKNRESALNVLRARLYAREQEKRQKELGESRL
CCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVGSGERAEKMRTYNYPQDRITDHRIGQNISNLPVVLDGALDRLIDALVTFDNAERLKA
HHCCCCHHHHHHHCCCCCHHHCCHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHH
LGMGA
HCCCC
>Mature Secondary Structure
MEPEILARLARVEARYIELNDEMTQPDALSDHVRLTTLAREQTELGELVGRYREFMQAEK
CCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
SVEEAKALLREENDPELREMAQLEIDELSARMEAIGAEVRVLLLPRDPNDEKNVIIEIRQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEC
GEGGDEAALFGADVYRMYQRYAEIRGWKTEVMSLSENGIGGFKEVIFEVQGQGAWSRLKY
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHH
EGGVHRVQRVPATEARGRIHTSTITVAVLPEVEETAVEIKQEDYRIDVYRSGGHGGQGVN
CCCHHHHHHCCCHHHCCEEEEEEEEEEECCCHHHHHHHHHHCCEEEEEEECCCCCCCCCC
TTDSAVRIVYKAGTPEEIVVVCQDTRSQIKNRESALNVLRARLYAREQEKRQKELGESRL
CCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVGSGERAEKMRTYNYPQDRITDHRIGQNISNLPVVLDGALDRLIDALVTFDNAERLKA
HHCCCCHHHHHHHCCCCCHHHCCHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHH
LGMGA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA