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The map label for this gene is mscS [H]

Identifier: 159899231

GI number: 159899231

Start: 3465507

End: 3467030

Strand: Direct

Name: mscS [H]

Synonym: Haur_2712

Alternate gene names: 159899231

Gene position: 3465507-3467030 (Clockwise)

Preceding gene: 159899230

Following gene: 159899236

Centisome position: 54.6

GC content: 50.59

Gene sequence:

>1524_bases
ATGTTAAGCGTCATTGAGCATATCATTCAGCCAATGCTGTGGGCAATTGGCACCTATGCAGTGTTGCGCCTGTTAATCCG
GCTTGGTCGGCGCATTGGGCTGATTAGCGGGTTAAGTATCCTTGGCCAATTTTTGGCGCTCAGTAGTGCTGGCTTAATTG
GCCTATGGTCGGATCAACAGCTTGAACATTGGCCGCTGCTCACTAAAATTTTGCAAACTACCGTGACTGTTTCGATTATT
GCGGTGGTTTTGCATATTGGTGATCAACTATTGGTGCGGCGTTTGGCTGCCGATGGTCGCCCTAACGCCATCCCGCGCTT
GATGCGTGATATTGCCCGTGGTTTTGTACTGCTTATGACCTTGCTGATTTGCATTGGCCATTTTTTCAAAGTGCAAATTG
GCACAGTGCTGCTCAGCTCGACCGTTTTTACCGCTGTGGTTGGCCTTGCCTTACAAGATTTGCTCAAAAATGTGATTGCT
GGGATTGCGCTGCAAATGGAGCGGCCTTTTATGCCTGGCGATTGGATTTTTATCGATGTGCAAACTGGCTATGGCCGCGT
TTTGGAGATGAGCTGGCGGGCGATTCGGGTCAAGCCGCGTGATGGACAGGTCGTGGTCATTCCCAACACGATTATTGCCC
AACAACAAATTATCAATATGAGTGCAACTGGCTTGCCTGTAGCCATGCGGGTTGCAGTAACCGTCGATTGTGTGCACCCG
CCAATGATGGTGCGCGAATTGTTGAAAGAAGCAGTACTTTCCAGTCGCGGGGTCGTCGCCCAGCCGGAACCATTTGTATT
TGTGCGCAACTATACTGCATTTTCCACAACCTACGAAGTTAAATGTTGGATCAGCAACTACGATGATTTTCCCCAGTTGC
AAGGTGATGCACTCAGCCGAATTTGGTATGTGTTCCAGCGTCAAGGCATTCACTTTGCCTCAAACGAAATGGTGCTGACC
CGTCCTGAACAAGCGAGTCGGCAACGCCGGCTTGATTATACCCCTGAGCAAATTTTCGACCGTTTGCGCCGAATCAAGCT
GCTCGATGGCTTGCACGAGCAAGAATTGCGTTTTTTATCGGAGCATGTCGAAATTCGCTTATTTGAGCGCGGCGAAATTC
TGGCCCATCAAGGCCGCCATGAACATATTTTGTATGCAATTACCCGCGGCAAAGTACGGGTTGAAGTTGCGCATGACGAT
CAGCCTGCAATTGTCGTCTCGCACTTAGGCGCTGGCGATGTGTTTGGCGAGCGTGGCATGTTAATGGATGAGCCACGTTC
GGCTAGCGTGATTGCCGAGGAAGATACGCGTACAATTGTGATCGAACGCCACGATTTGGCTCCGCTGTATGCCAAAAATC
CCTCATTGGCTGAACGTTTGGGGGTTATGGTAGCCGAGCGCCAACAAGCTACCCACAATTATTTAGAGCAGCAACGCTCG
GTCAACCAAAACCCCACGCCATCGCCTGCTGCCCGCTCAATCACCGAGCGCATCCGCGATGTGCTGCTCGATTTGAGCAA
ATAA

Upstream 100 bases:

>100_bases
CAATTGTGCTTATCGCCATGTGGGCATTGGCCTAGCCTACGTTGGCGACCACCCGTATTGGGTGCTTGACATGGCCGAAC
CAAAACCCTAAGATGCAGTT

Downstream 100 bases:

>100_bases
CTACAACGCTTGAAAATGACCAACCAAAGCGGCATAAGTATCATCCATGGCTTCGGGAATGAGCTTGACCCCACCAATTA
ATGGCATAAAGTTGGTATCG

Product: cyclic nucleotide-regulated small mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLSVIEHIIQPMLWAIGTYAVLRLLIRLGRRIGLISGLSILGQFLALSSAGLIGLWSDQQLEHWPLLTKILQTTVTVSII
AVVLHIGDQLLVRRLAADGRPNAIPRLMRDIARGFVLLMTLLICIGHFFKVQIGTVLLSSTVFTAVVGLALQDLLKNVIA
GIALQMERPFMPGDWIFIDVQTGYGRVLEMSWRAIRVKPRDGQVVVIPNTIIAQQQIINMSATGLPVAMRVAVTVDCVHP
PMMVRELLKEAVLSSRGVVAQPEPFVFVRNYTAFSTTYEVKCWISNYDDFPQLQGDALSRIWYVFQRQGIHFASNEMVLT
RPEQASRQRRLDYTPEQIFDRLRRIKLLDGLHEQELRFLSEHVEIRLFERGEILAHQGRHEHILYAITRGKVRVEVAHDD
QPAIVVSHLGAGDVFGERGMLMDEPRSASVIAEEDTRTIVIERHDLAPLYAKNPSLAERLGVMVAERQQATHNYLEQQRS
VNQNPTPSPAARSITERIRDVLLDLSK

Sequences:

>Translated_507_residues
MLSVIEHIIQPMLWAIGTYAVLRLLIRLGRRIGLISGLSILGQFLALSSAGLIGLWSDQQLEHWPLLTKILQTTVTVSII
AVVLHIGDQLLVRRLAADGRPNAIPRLMRDIARGFVLLMTLLICIGHFFKVQIGTVLLSSTVFTAVVGLALQDLLKNVIA
GIALQMERPFMPGDWIFIDVQTGYGRVLEMSWRAIRVKPRDGQVVVIPNTIIAQQQIINMSATGLPVAMRVAVTVDCVHP
PMMVRELLKEAVLSSRGVVAQPEPFVFVRNYTAFSTTYEVKCWISNYDDFPQLQGDALSRIWYVFQRQGIHFASNEMVLT
RPEQASRQRRLDYTPEQIFDRLRRIKLLDGLHEQELRFLSEHVEIRLFERGEILAHQGRHEHILYAITRGKVRVEVAHDD
QPAIVVSHLGAGDVFGERGMLMDEPRSASVIAEEDTRTIVIERHDLAPLYAKNPSLAERLGVMVAERQQATHNYLEQQRS
VNQNPTPSPAARSITERIRDVLLDLSK
>Mature_507_residues
MLSVIEHIIQPMLWAIGTYAVLRLLIRLGRRIGLISGLSILGQFLALSSAGLIGLWSDQQLEHWPLLTKILQTTVTVSII
AVVLHIGDQLLVRRLAADGRPNAIPRLMRDIARGFVLLMTLLICIGHFFKVQIGTVLLSSTVFTAVVGLALQDLLKNVIA
GIALQMERPFMPGDWIFIDVQTGYGRVLEMSWRAIRVKPRDGQVVVIPNTIIAQQQIINMSATGLPVAMRVAVTVDCVHP
PMMVRELLKEAVLSSRGVVAQPEPFVFVRNYTAFSTTYEVKCWISNYDDFPQLQGDALSRIWYVFQRQGIHFASNEMVLT
RPEQASRQRRLDYTPEQIFDRLRRIKLLDGLHEQELRFLSEHVEIRLFERGEILAHQGRHEHILYAITRGKVRVEVAHDD
QPAIVVSHLGAGDVFGERGMLMDEPRSASVIAEEDTRTIVIERHDLAPLYAKNPSLAERLGVMVAERQQATHNYLEQQRS
VNQNPTPSPAARSITERIRDVLLDLSK

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=265, Percent_Identity=27.9245283018868, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787591, Length=266, Percent_Identity=24.812030075188, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 57215; Mature: 57215

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVIEHIIQPMLWAIGTYAVLRLLIRLGRRIGLISGLSILGQFLALSSAGLIGLWSDQQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LEHWPLLTKILQTTVTVSIIAVVLHIGDQLLVRRLAADGRPNAIPRLMRDIARGFVLLMT
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LLICIGHFFKVQIGTVLLSSTVFTAVVGLALQDLLKNVIAGIALQMERPFMPGDWIFIDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEE
QTGYGRVLEMSWRAIRVKPRDGQVVVIPNTIIAQQQIINMSATGLPVAMRVAVTVDCVHP
ECCCCCEEEEEEEEEEEECCCCEEEEECCHHHHHHHHHCCCCCCCCEEEEEEEEEECCCC
PMMVRELLKEAVLSSRGVVAQPEPFVFVRNYTAFSTTYEVKCWISNYDDFPQLQGDALSR
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECEEEEEEEEEEEEECCCCCCCCCCHHHHHH
IWYVFQRQGIHFASNEMVLTRPEQASRQRRLDYTPEQIFDRLRRIKLLDGLHEQELRFLS
HHHHHHHCCCEEECCCEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
EHVEIRLFERGEILAHQGRHEHILYAITRGKVRVEVAHDDQPAIVVSHLGAGDVFGERGM
HHHEEEEEECCCHHHCCCCCCEEEEEEECCEEEEEEECCCCCEEEEEECCCCCCCCCCCC
LMDEPRSASVIAEEDTRTIVIERHDLAPLYAKNPSLAERLGVMVAERQQATHNYLEQQRS
CCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
VNQNPTPSPAARSITERIRDVLLDLSK
CCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSVIEHIIQPMLWAIGTYAVLRLLIRLGRRIGLISGLSILGQFLALSSAGLIGLWSDQQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LEHWPLLTKILQTTVTVSIIAVVLHIGDQLLVRRLAADGRPNAIPRLMRDIARGFVLLMT
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LLICIGHFFKVQIGTVLLSSTVFTAVVGLALQDLLKNVIAGIALQMERPFMPGDWIFIDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEE
QTGYGRVLEMSWRAIRVKPRDGQVVVIPNTIIAQQQIINMSATGLPVAMRVAVTVDCVHP
ECCCCCEEEEEEEEEEEECCCCEEEEECCHHHHHHHHHCCCCCCCCEEEEEEEEEECCCC
PMMVRELLKEAVLSSRGVVAQPEPFVFVRNYTAFSTTYEVKCWISNYDDFPQLQGDALSR
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECEEEEEEEEEEEEECCCCCCCCCCHHHHHH
IWYVFQRQGIHFASNEMVLTRPEQASRQRRLDYTPEQIFDRLRRIKLLDGLHEQELRFLS
HHHHHHHCCCEEECCCEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
EHVEIRLFERGEILAHQGRHEHILYAITRGKVRVEVAHDDQPAIVVSHLGAGDVFGERGM
HHHEEEEEECCCHHHCCCCCCEEEEEEECCEEEEEEECCCCCEEEEEECCCCCCCCCCCC
LMDEPRSASVIAEEDTRTIVIERHDLAPLYAKNPSLAERLGVMVAERQQATHNYLEQQRS
CCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
VNQNPTPSPAARSITERIRDVLLDLSK
CCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]