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The map label for this gene is mdlB [H]

Identifier: 159898395

GI number: 159898395

Start: 2212472

End: 2213548

Strand: Reverse

Name: mdlB [H]

Synonym: Haur_1871

Alternate gene names: 159898395

Gene position: 2213548-2212472 (Counterclockwise)

Preceding gene: 159898407

Following gene: 159898394

Centisome position: 34.88

GC content: 51.44

Gene sequence:

>1077_bases
ATGCCACCAATTAACCTCATGGATTACCAGCCATTGGCCAAGCAACTGATTGATCGATCAGCTTGGGATTATATTCAGGG
TGGCAGCGATGATGAAATCACGTTGCAGGCCAACCAAGCCGCTTATACCAAGTTGAAGCTACGCCCGCGAGTCTTGGTTG
ATGTTAGTCAGTGCACATTAGAAACAAGCGTGCTTGGCCAAACAATCGCAATGCCGATTGGCATCGCCCCAATGGGTTGC
CAAGGCTTAGTTCATGCTGAAGGCGAGTGTGCTATGGCGCGAGCAGCCGAAGCAGCTCAGACCGTGATGATTGCTAGTGC
CATGGCCAACTACTCGCTTGAAGCGATTGCCCAAGCCGCGAACGGCCCCCTGTGGTTTCAACTGTATGTGTACCGCGAAC
GCCAGATTACTGAAGCGTTAGTACGACGGGTCGAGGCTGCTGGCTATCAGGCTTTAGTGCTCACTGTTGATGTCCCATTT
TTGGGCCGTCGCGAGCGTGATTTACGCAATGGCTTTGCCTTACCGCAACACTTGCATTTTGCGAATTTTGCACCAACTGA
TGCCGCAGGCCAACATCAACAAACGCTTGGAGCTTCAGGGATTGCAACGCATGCGGCTGGACGCTTTGACGCAGCATTAA
CCTGGGAAGCGATTGACTGGTTACGCTCACTTACGCGGTTGCCAATTGTGTTGAAAGGCATTTTAAGTGCTGAAGATGCC
CAATTGGCAGTGCAACACGGCGTTGATGGGCTGATTGTGTCAAACCATGGTGGACGGCAATTGGATACGGTTGCGGCAAC
TATTGAATGCCTACCTGCAATTGTTGATGCCGTCGGCTCGACCTGCGAAGTGTATCTTGATGGCGGTATTCGCCGGGGAA
CCGATGTGCTCAAGGCGCTCGCCTTGGGCGCAAAGATGGTTTTTGTGGGACGACCCTTGTTATGGGGTTTGGCGGTTGAT
GGGCAGCAAGGCGCACACCATGTGTTGGAATTACTCAGATCTGAATATAGCTTAGCCTTAGGATTAATCGGCTGTCCCCA
CAGTCATCAATTAAATCGACATTATATTAGCTCCTAA

Upstream 100 bases:

>100_bases
AATCTGCTATACTAGCGCCACACCCAAACAACACTAAATCAACTGCTCGACTCAATTTGCATTTAAGTAGATCAATTCTA
TCAACGGGAATTAACGTCTT

Downstream 100 bases:

>100_bases
GCCTCAATTCCTCCGATACCAAATCAATCCCCTGACAGATTAAGTTCGTTGAACACGACTCATCGCCGCCCGTTTATTTA
AATGACGGGCGGGTTATTTC

Product: (S)-2-hydroxy-acid oxidase

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MPPINLMDYQPLAKQLIDRSAWDYIQGGSDDEITLQANQAAYTKLKLRPRVLVDVSQCTLETSVLGQTIAMPIGIAPMGC
QGLVHAEGECAMARAAEAAQTVMIASAMANYSLEAIAQAANGPLWFQLYVYRERQITEALVRRVEAAGYQALVLTVDVPF
LGRRERDLRNGFALPQHLHFANFAPTDAAGQHQQTLGASGIATHAAGRFDAALTWEAIDWLRSLTRLPIVLKGILSAEDA
QLAVQHGVDGLIVSNHGGRQLDTVAATIECLPAIVDAVGSTCEVYLDGGIRRGTDVLKALALGAKMVFVGRPLLWGLAVD
GQQGAHHVLELLRSEYSLALGLIGCPHSHQLNRHYISS

Sequences:

>Translated_358_residues
MPPINLMDYQPLAKQLIDRSAWDYIQGGSDDEITLQANQAAYTKLKLRPRVLVDVSQCTLETSVLGQTIAMPIGIAPMGC
QGLVHAEGECAMARAAEAAQTVMIASAMANYSLEAIAQAANGPLWFQLYVYRERQITEALVRRVEAAGYQALVLTVDVPF
LGRRERDLRNGFALPQHLHFANFAPTDAAGQHQQTLGASGIATHAAGRFDAALTWEAIDWLRSLTRLPIVLKGILSAEDA
QLAVQHGVDGLIVSNHGGRQLDTVAATIECLPAIVDAVGSTCEVYLDGGIRRGTDVLKALALGAKMVFVGRPLLWGLAVD
GQQGAHHVLELLRSEYSLALGLIGCPHSHQLNRHYISS
>Mature_357_residues
PPINLMDYQPLAKQLIDRSAWDYIQGGSDDEITLQANQAAYTKLKLRPRVLVDVSQCTLETSVLGQTIAMPIGIAPMGCQ
GLVHAEGECAMARAAEAAQTVMIASAMANYSLEAIAQAANGPLWFQLYVYRERQITEALVRRVEAAGYQALVLTVDVPFL
GRRERDLRNGFALPQHLHFANFAPTDAAGQHQQTLGASGIATHAAGRFDAALTWEAIDWLRSLTRLPIVLKGILSAEDAQ
LAVQHGVDGLIVSNHGGRQLDTVAATIECLPAIVDAVGSTCEVYLDGGIRRGTDVLKALALGAKMVFVGRPLLWGLAVDG
QQGAHHVLELLRSEYSLALGLIGCPHSHQLNRHYISS

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI11068137, Length=355, Percent_Identity=47.3239436619718, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI54234014, Length=357, Percent_Identity=45.6582633053221, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI7705393, Length=357, Percent_Identity=45.6582633053221, Blast_Score=312, Evalue=3e-85,
Organism=Escherichia coli, GI1790033, Length=365, Percent_Identity=35.0684931506849, Blast_Score=228, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI193208036, Length=363, Percent_Identity=43.801652892562, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI32566217, Length=275, Percent_Identity=41.4545454545455, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6323587, Length=360, Percent_Identity=38.3333333333333, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI78707188, Length=348, Percent_Identity=47.9885057471264, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI281363140, Length=348, Percent_Identity=47.9885057471264, Blast_Score=329, Evalue=1e-90,
Organism=Drosophila melanogaster, GI78707190, Length=348, Percent_Identity=47.9885057471264, Blast_Score=329, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 38600; Mature: 38469

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00557 FMN_HYDROXY_ACID_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPINLMDYQPLAKQLIDRSAWDYIQGGSDDEITLQANQAAYTKLKLRPRVLVDVSQCTL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCEEEEEHHHHHH
ETSVLGQTIAMPIGIAPMGCQGLVHAEGECAMARAAEAAQTVMIASAMANYSLEAIAQAA
HHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
NGPLWFQLYVYRERQITEALVRRVEAAGYQALVLTVDVPFLGRRERDLRNGFALPQHLHF
CCCEEEEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCCCCCHHCCC
ANFAPTDAAGQHQQTLGASGIATHAAGRFDAALTWEAIDWLRSLTRLPIVLKGILSAEDA
CCCCCCCCCCCHHHHCCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCH
QLAVQHGVDGLIVSNHGGRQLDTVAATIECLPAIVDAVGSTCEVYLDGGIRRGTDVLKAL
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
ALGAKMVFVGRPLLWGLAVDGQQGAHHVLELLRSEYSLALGLIGCPHSHQLNRHYISS
HHCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCHHCCCC
>Mature Secondary Structure 
PPINLMDYQPLAKQLIDRSAWDYIQGGSDDEITLQANQAAYTKLKLRPRVLVDVSQCTL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCEEEEEHHHHHH
ETSVLGQTIAMPIGIAPMGCQGLVHAEGECAMARAAEAAQTVMIASAMANYSLEAIAQAA
HHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
NGPLWFQLYVYRERQITEALVRRVEAAGYQALVLTVDVPFLGRRERDLRNGFALPQHLHF
CCCEEEEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCCCCCHHCCC
ANFAPTDAAGQHQQTLGASGIATHAAGRFDAALTWEAIDWLRSLTRLPIVLKGILSAEDA
CCCCCCCCCCCHHHHCCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCH
QLAVQHGVDGLIVSNHGGRQLDTVAATIECLPAIVDAVGSTCEVYLDGGIRRGTDVLKAL
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
ALGAKMVFVGRPLLWGLAVDGQQGAHHVLELLRSEYSLALGLIGCPHSHQLNRHYISS
HHCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]