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The map label for this gene is ligT [C]

Identifier: 159898160

GI number: 159898160

Start: 1893107

End: 1893694

Strand: Reverse

Name: ligT [C]

Synonym: Haur_1636

Alternate gene names: 159898160

Gene position: 1893694-1893107 (Counterclockwise)

Preceding gene: 159898161

Following gene: 159898158

Centisome position: 29.84

GC content: 48.81

Gene sequence:

>588_bases
ATGAGCGAGCAATGGCGGTGTTTTATGGCGCTCGATTTGCCCGAGCCAATTCAGGCGCAACTCTTGGCGATCCAAGCCGA
ATTACAGCAATTGGGCTTGCCAATTCGTTGGAGCAAGCCCGAAAATCTGCATTTAACCTTGCAATTTTTGGGCAATCTAG
CGGTTGAATTATTGCCCGATTTACAAACCAAGCTAAATCATGCCCTCACACAGCAAACTGCACCAACCCTCAATTGCAAT
ACATGGGGTTATTTTCCCAATGCCAGCCAACCGCAAGTCGTCTGGGCAGGTTTTGAGCAATGGCCCAACGATTTGCTGCA
ACTTCAACAAGCAGTCGTTGGGTGCAGCAGCCACATTGGCATCATTGCCGAAACCCGCCCATGGCATCCCCACATCACCT
TGGGCTACAGCAAAGAGCGTTTAACCAAACACCAAATTGAACAATTATCAAGCCAATTGCAAGCCAGCCAATGGCCAGCA
ACACCAGCGATCTACCCAACTTGTCAACTTATACGCAGCATAAGCCAACCTCAAGCCAGCAGCTACCAAACTTTAGTGAC
ATGGCAGCTTAACCAAGCATCAACCTAA

Upstream 100 bases:

>100_bases
CTTAATGATTGGCTTGTACCCGCCGTTGGTACTCGACCCAATTATTGAAGCAGTTCGCGGCCTAACATTTGTACAATTCC
TCTAAGCCCAAGGATTGCCT

Downstream 100 bases:

>100_bases
AATGCATGGTTCAATCATCAAGATCAAAGCAATTATTCAATCGTAATGAATGGCTCAATTTCAGCAAAAGTGCTTGCCCC
AATCCCCTTGACCTCTTGTA

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSEQWRCFMALDLPEPIQAQLLAIQAELQQLGLPIRWSKPENLHLTLQFLGNLAVELLPDLQTKLNHALTQQTAPTLNCN
TWGYFPNASQPQVVWAGFEQWPNDLLQLQQAVVGCSSHIGIIAETRPWHPHITLGYSKERLTKHQIEQLSSQLQASQWPA
TPAIYPTCQLIRSISQPQASSYQTLVTWQLNQAST

Sequences:

>Translated_195_residues
MSEQWRCFMALDLPEPIQAQLLAIQAELQQLGLPIRWSKPENLHLTLQFLGNLAVELLPDLQTKLNHALTQQTAPTLNCN
TWGYFPNASQPQVVWAGFEQWPNDLLQLQQAVVGCSSHIGIIAETRPWHPHITLGYSKERLTKHQIEQLSSQLQASQWPA
TPAIYPTCQLIRSISQPQASSYQTLVTWQLNQAST
>Mature_194_residues
SEQWRCFMALDLPEPIQAQLLAIQAELQQLGLPIRWSKPENLHLTLQFLGNLAVELLPDLQTKLNHALTQQTAPTLNCNT
WGYFPNASQPQVVWAGFEQWPNDLLQLQQAVVGCSSHIGIIAETRPWHPHITLGYSKERLTKHQIEQLSSQLQASQWPAT
PAIYPTCQLIRSISQPQASSYQTLVTWQLNQAST

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

Organism=Escherichia coli, GI87081692, Length=136, Percent_Identity=34.5588235294118, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 22056; Mature: 21925

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQWRCFMALDLPEPIQAQLLAIQAELQQLGLPIRWSKPENLHLTLQFLGNLAVELLPD
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHH
LQTKLNHALTQQTAPTLNCNTWGYFPNASQPQVVWAGFEQWPNDLLQLQQAVVGCSSHIG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECHHHCHHHHHHHHHHHHCCCCCCE
IIAETRPWHPHITLGYSKERLTKHQIEQLSSQLQASQWPATPAIYPTCQLIRSISQPQAS
EEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCC
SYQTLVTWQLNQAST
CCEEEEEEECCCCCC
>Mature Secondary Structure 
SEQWRCFMALDLPEPIQAQLLAIQAELQQLGLPIRWSKPENLHLTLQFLGNLAVELLPD
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHH
LQTKLNHALTQQTAPTLNCNTWGYFPNASQPQVVWAGFEQWPNDLLQLQQAVVGCSSHIG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECHHHCHHHHHHHHHHHHCCCCCCE
IIAETRPWHPHITLGYSKERLTKHQIEQLSSQLQASQWPATPAIYPTCQLIRSISQPQAS
EEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCC
SYQTLVTWQLNQAST
CCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]