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The map label for this gene is lmrB [H]

Identifier: 159897984

GI number: 159897984

Start: 1701125

End: 1702576

Strand: Reverse

Name: lmrB [H]

Synonym: Haur_1459

Alternate gene names: 159897984

Gene position: 1702576-1701125 (Counterclockwise)

Preceding gene: 159897985

Following gene: 159897981

Centisome position: 26.83

GC content: 51.58

Gene sequence:

>1452_bases
ATGCGCGTAGAGACAACCCATGCCAGCGCCGTTGCAACCAGCACCCGCGAAAAAGGCATTATTTTAACCTTGTTGATTGC
GACCTTTGTCGTGATTCTAAACGAAACGATTATGGGTGTGGCTTTACCCCGCTTGAAGGACGAATTCCAAGTCGAAGCAA
GCACCGTGCAATGGCTGACCACAGCCTATCTATTAGTTATGGCGGTCTTGATTCCCACAACCGGCTTTCTGATTCAACGG
TTTAGCACGCGAACGCTGTTTCTTACGGCCATGGGTTTATTTACCTTCGGGACTGTGGCAGCGGCAGCAGCGCCTGGATT
TGAAGTGCTCTTGGTTGGTCGGGTATTCCAAGCAGCGGGCACAGCGCTAATGCTGCCATTGCTCATGACAGTTATTTTGG
TGCTAGTGCCGATGGAGCGGCGCGGAGCTATGATGAGCACGGCCAGTATGGTGATTTCGGTGGCTCCCGCGATTGGCCCA
GCCCTATCAGGGTTAATCATCCAAGCGCTTTCGTGGCGTTTTATGTTTATTTTCGTGATCCCAATTGCCCTAGCGGTGCT
GGCCTATGGCTACCTACGGTTGGTGAATGTGGGCAAACTTCAAGCAGCCAGCCTTGATCGTACCTCGATTCTCTTAGCAG
CAATAGGCTTCGGCGGGCTAGTCTACGGCTTTAGTCGGGCGGGCGAGCCTGGCAGTAGCCTAACCGAGCCAATTGTTTTG
GCAGCGATCGGAATGGGCTTAGTTGGGATCGCATTGTTTGCATGGCGACAAACCCGACTTTCCAGTCCATTGCTTGACCT
GCGCGTTTTTCGCTTTCCCATGTTCACGCTGAGCGTCGTATTAATGATGGTTGTGATGGTGACATTATTTGCCTCAGCGA
TTCTATTGCCCTTCTATTTCCAGCAAATTCGCCAGTTTAATACCCTGCAAACCGGGCTTTTTTTGCTGCCTGGCGGGGCA
TTGATGGGATTCAGTGGGCTATTCGTAGGTCGGATCTTTGATCGATCGGGGCCACGAATGCTCGTGATCGGGGGCATGGT
ACTGGTTGTCTTGGCACTTTGGCAGTGGACGTTCTTGAATGCCACAACCTCAGTGATCTGGCTTCTCAGTCTCCATGTAA
TCTTCAGTTTAGGCCTTGCCTGTTTGTTCACGCCAATTCTCACAACTGGACTTAATCAATTGCCCCAAACCTTGCACTCC
CATGGCACAGCGATCTCCAACACACTTCAGCAAGTTGCCGGAGCAATTGGAACGGCCTTATTAATCACGATTATGAGTGC
TGGTCAAGCTCAGCCAGGGCAAACCATCGCGCCTAATCCATTGGCTGATTTGACCATTGGCATCCAAAATGCCTTCGTGG
TGGCAGCTGGAATAGCCGTGGTTGGTTTGGTGTTAGCGCTTTTTCTGCGTCGCACGGGCGAACCAAGCGGAGAGCCAACA
TTTATTCACTAA

Upstream 100 bases:

>100_bases
TGATGGCGGCGTTGCACTGCATCGCTATGCCGTAGCTCATCATCGTGATTAAATTCAGCTCATCCGCTAGGCTTTACGGC
GGGTTATCAAAAGGAATTCT

Downstream 100 bases:

>100_bases
TGGCACTGAATAGGCATGTTGGAGCTAAAAATCAACAGCCGAGGCAAAACCTCGGCTGTTATAGTTAACATCTGCTTGCG
CTAGCTCAACCACTGGGCTA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MRVETTHASAVATSTREKGIILTLLIATFVVILNETIMGVALPRLKDEFQVEASTVQWLTTAYLLVMAVLIPTTGFLIQR
FSTRTLFLTAMGLFTFGTVAAAAAPGFEVLLVGRVFQAAGTALMLPLLMTVILVLVPMERRGAMMSTASMVISVAPAIGP
ALSGLIIQALSWRFMFIFVIPIALAVLAYGYLRLVNVGKLQAASLDRTSILLAAIGFGGLVYGFSRAGEPGSSLTEPIVL
AAIGMGLVGIALFAWRQTRLSSPLLDLRVFRFPMFTLSVVLMMVVMVTLFASAILLPFYFQQIRQFNTLQTGLFLLPGGA
LMGFSGLFVGRIFDRSGPRMLVIGGMVLVVLALWQWTFLNATTSVIWLLSLHVIFSLGLACLFTPILTTGLNQLPQTLHS
HGTAISNTLQQVAGAIGTALLITIMSAGQAQPGQTIAPNPLADLTIGIQNAFVVAAGIAVVGLVLALFLRRTGEPSGEPT
FIH

Sequences:

>Translated_483_residues
MRVETTHASAVATSTREKGIILTLLIATFVVILNETIMGVALPRLKDEFQVEASTVQWLTTAYLLVMAVLIPTTGFLIQR
FSTRTLFLTAMGLFTFGTVAAAAAPGFEVLLVGRVFQAAGTALMLPLLMTVILVLVPMERRGAMMSTASMVISVAPAIGP
ALSGLIIQALSWRFMFIFVIPIALAVLAYGYLRLVNVGKLQAASLDRTSILLAAIGFGGLVYGFSRAGEPGSSLTEPIVL
AAIGMGLVGIALFAWRQTRLSSPLLDLRVFRFPMFTLSVVLMMVVMVTLFASAILLPFYFQQIRQFNTLQTGLFLLPGGA
LMGFSGLFVGRIFDRSGPRMLVIGGMVLVVLALWQWTFLNATTSVIWLLSLHVIFSLGLACLFTPILTTGLNQLPQTLHS
HGTAISNTLQQVAGAIGTALLITIMSAGQAQPGQTIAPNPLADLTIGIQNAFVVAAGIAVVGLVLALFLRRTGEPSGEPT
FIH
>Mature_483_residues
MRVETTHASAVATSTREKGIILTLLIATFVVILNETIMGVALPRLKDEFQVEASTVQWLTTAYLLVMAVLIPTTGFLIQR
FSTRTLFLTAMGLFTFGTVAAAAAPGFEVLLVGRVFQAAGTALMLPLLMTVILVLVPMERRGAMMSTASMVISVAPAIGP
ALSGLIIQALSWRFMFIFVIPIALAVLAYGYLRLVNVGKLQAASLDRTSILLAAIGFGGLVYGFSRAGEPGSSLTEPIVL
AAIGMGLVGIALFAWRQTRLSSPLLDLRVFRFPMFTLSVVLMMVVMVTLFASAILLPFYFQQIRQFNTLQTGLFLLPGGA
LMGFSGLFVGRIFDRSGPRMLVIGGMVLVVLALWQWTFLNATTSVIWLLSLHVIFSLGLACLFTPILTTGLNQLPQTLHS
HGTAISNTLQQVAGAIGTALLITIMSAGQAQPGQTIAPNPLADLTIGIQNAFVVAAGIAVVGLVLALFLRRTGEPSGEPT
FIH

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=408, Percent_Identity=24.5098039215686, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1789042, Length=433, Percent_Identity=26.0969976905312, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790195, Length=415, Percent_Identity=26.2650602409639, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081983, Length=308, Percent_Identity=25.6493506493506, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=27.6119402985075, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51541; Mature: 51541

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVETTHASAVATSTREKGIILTLLIATFVVILNETIMGVALPRLKDEFQVEASTVQWLT
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAYLLVMAVLIPTTGFLIQRFSTRTLFLTAMGLFTFGTVAAAAAPGFEVLLVGRVFQAAG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TALMLPLLMTVILVLVPMERRGAMMSTASMVISVAPAIGPALSGLIIQALSWRFMFIFVI
HHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIALAVLAYGYLRLVNVGKLQAASLDRTSILLAAIGFGGLVYGFSRAGEPGSSLTEPIVL
HHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AAIGMGLVGIALFAWRQTRLSSPLLDLRVFRFPMFTLSVVLMMVVMVTLFASAILLPFYF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQIRQFNTLQTGLFLLPGGALMGFSGLFVGRIFDRSGPRMLVIGGMVLVVLALWQWTFLN
HHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHH
ATTSVIWLLSLHVIFSLGLACLFTPILTTGLNQLPQTLHSHGTAISNTLQQVAGAIGTAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LITIMSAGQAQPGQTIAPNPLADLTIGIQNAFVVAAGIAVVGLVLALFLRRTGEPSGEPT
HHHHHHCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FIH
CCC
>Mature Secondary Structure
MRVETTHASAVATSTREKGIILTLLIATFVVILNETIMGVALPRLKDEFQVEASTVQWLT
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAYLLVMAVLIPTTGFLIQRFSTRTLFLTAMGLFTFGTVAAAAAPGFEVLLVGRVFQAAG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TALMLPLLMTVILVLVPMERRGAMMSTASMVISVAPAIGPALSGLIIQALSWRFMFIFVI
HHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIALAVLAYGYLRLVNVGKLQAASLDRTSILLAAIGFGGLVYGFSRAGEPGSSLTEPIVL
HHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AAIGMGLVGIALFAWRQTRLSSPLLDLRVFRFPMFTLSVVLMMVVMVTLFASAILLPFYF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQIRQFNTLQTGLFLLPGGALMGFSGLFVGRIFDRSGPRMLVIGGMVLVVLALWQWTFLN
HHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHH
ATTSVIWLLSLHVIFSLGLACLFTPILTTGLNQLPQTLHSHGTAISNTLQQVAGAIGTAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LITIMSAGQAQPGQTIAPNPLADLTIGIQNAFVVAAGIAVVGLVLALFLRRTGEPSGEPT
HHHHHHCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FIH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]