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The map label for this gene is pleD [H]

Identifier: 159897749

GI number: 159897749

Start: 1411398

End: 1411790

Strand: Reverse

Name: pleD [H]

Synonym: Haur_1220

Alternate gene names: 159897749

Gene position: 1411790-1411398 (Counterclockwise)

Preceding gene: 159897757

Following gene: 159897742

Centisome position: 22.24

GC content: 47.84

Gene sequence:

>393_bases
ATGCCAACCATCTTAGTTGTTGATGATATGCCTGACAACCGTCAGCTTTTAGGAATAATGCTCCAACGGTCTGGTTATAC
AACCGAGATGGCTCGTGATGGGGTTGAAGCTCTGGAAGCCATTCAAAAAAGCCGACCAGACCTTATATTGCTTGATTTGA
ACTTGCCACGCATGGATGGCTGGGCAGTCTGTCGCACCGTTAAATCCGACCCTCGCTTGGCGCATATTCCGATTGTGGCA
TTAACTGCTAGTTGCAGCCCTGGCGAAGTCAAGCAATTGATGACCCCTGATTGGACCGATTATGTGAGCAAGCCTTTCGA
CGTTATGTCACTAATGGAGAAGGTTAAGCTCTGGCTGACTGGTTCCAATCAATCCAAGCCATGGTATGGTTAA

Upstream 100 bases:

>100_bases
TCGACCAAGAGTCCTACCTGTAAATTGGCTAGTAAGGTTGTATCGCTATAGTACGTTTGGATACGAGTAACGAGAAGGCC
CAAATTTAGGAGATACCCAT

Downstream 100 bases:

>100_bases
GCTTTCATCCATATAATCAAACTTAAATAAATAGCGCTAGTTGAATTGCTTCAACTAGCGCTTTATCGTATCTTTGGTTG
ATTGTAGGTTTAGGTCGAGG

Product: response regulator receiver protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MPTILVVDDMPDNRQLLGIMLQRSGYTTEMARDGVEALEAIQKSRPDLILLDLNLPRMDGWAVCRTVKSDPRLAHIPIVA
LTASCSPGEVKQLMTPDWTDYVSKPFDVMSLMEKVKLWLTGSNQSKPWYG

Sequences:

>Translated_130_residues
MPTILVVDDMPDNRQLLGIMLQRSGYTTEMARDGVEALEAIQKSRPDLILLDLNLPRMDGWAVCRTVKSDPRLAHIPIVA
LTASCSPGEVKQLMTPDWTDYVSKPFDVMSLMEKVKLWLTGSNQSKPWYG
>Mature_129_residues
PTILVVDDMPDNRQLLGIMLQRSGYTTEMARDGVEALEAIQKSRPDLILLDLNLPRMDGWAVCRTVKSDPRLAHIPIVAL
TASCSPGEVKQLMTPDWTDYVSKPFDVMSLMEKVKLWLTGSNQSKPWYG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=32.3809523809524, Blast_Score=73, Evalue=7e-15,
Organism=Escherichia coli, GI1788191, Length=113, Percent_Identity=30.9734513274336, Blast_Score=69, Evalue=9e-14,
Organism=Escherichia coli, GI145693157, Length=123, Percent_Identity=30.8943089430894, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1788394, Length=120, Percent_Identity=35.8333333333333, Blast_Score=66, Evalue=6e-13,
Organism=Escherichia coli, GI1788713, Length=87, Percent_Identity=37.9310344827586, Blast_Score=60, Evalue=5e-11,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=30.5555555555556, Blast_Score=59, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 14619; Mature: 14488

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTILVVDDMPDNRQLLGIMLQRSGYTTEMARDGVEALEAIQKSRPDLILLDLNLPRMDG
CCEEEEEECCCCCHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
WAVCRTVKSDPRLAHIPIVALTASCSPGEVKQLMTPDWTDYVSKPFDVMSLMEKVKLWLT
HHHHEECCCCCCEEECEEEEEECCCCCHHHHHHCCCCHHHHHCCCHHHHHHHHHHEEEEE
GSNQSKPWYG
CCCCCCCCCC
>Mature Secondary Structure 
PTILVVDDMPDNRQLLGIMLQRSGYTTEMARDGVEALEAIQKSRPDLILLDLNLPRMDG
CEEEEEECCCCCHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
WAVCRTVKSDPRLAHIPIVALTASCSPGEVKQLMTPDWTDYVSKPFDVMSLMEKVKLWLT
HHHHEECCCCCCEEECEEEEEECCCCCHHHHHHCCCCHHHHHCCCHHHHHHHHHHEEEEE
GSNQSKPWYG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA