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The map label for this gene is dfrA [H]

Identifier: 159897736

GI number: 159897736

Start: 1390320

End: 1391339

Strand: Direct

Name: dfrA [H]

Synonym: Haur_1207

Alternate gene names: 159897736

Gene position: 1390320-1391339 (Clockwise)

Preceding gene: 159897735

Following gene: 159897738

Centisome position: 21.91

GC content: 50.1

Gene sequence:

>1020_bases
ATGAGCAAGCGCGTGTTGGTTACCGGAGCGAATGGTCATTTAGGCTCAGTTTTGGCTCAAATGTTGGTGGAGCGTGGGGT
TGATGTGCGGGCCAGCGTGCGCAATCGCAGCCAAATCAAGCCGCAACTGGCCTATGAGCAAGTGTATGCCGATTTGATGG
ATATGGATTCGCTGCAACAAGCCTTGGTTGGAGTTGATACACTCTATCAAGTAGCGGCGGTATTCAAGCATTGGTCGCGC
AATCCGCAACGCGAGATCATTCAACCAAACGTTGAGGGCACACGCAATATTTTGCGAGCAGCGGCGCAGGCGGGGGTCAA
ACGGGTGGTGTATGTCAGTTCAATCGCGGCAGTCGATAAAAATAATCCTCAGCGCCAAATCCCAGCAGATGAAACAACCT
GGAACCAATATACCTATGGTAATCCGTATTATCAATCGAAAATCGCCTCGGAACAATTGGCGTGGAAGTTGGCCAAGGAA
TATGGCTTGGAGATGATGGCGGGCTTGCCAGGCACGATCATCGGCGATCCCAATGGCCGTACCACACCATCGTTGGGGAT
TTTGGAGTTGGTGTTGAGCAATAAAATGCCACTAGATATCAATATGGATTTTAATTTTGTCGATGTGGCTGATGTTGCCG
AAGGGCTGATCGCTGCTGAGCGCCAAGGTCGGGCAGGCGAACGCTATATTTTGGCGAATGATCAATCGTTACCATTGCGA
CGAATCTTTGAAATCGCCCAAGAGTTTAATCCCAAAATCAAAGTGCCAATGCGGGTATCTAAAGGCATTACCAATGTCGT
AGCTGGCATGATGGAGTTGGTGGCGAATGTCACAGGCCGCGAACCAATGATTTTGCGCAGCCAAGTTGGGCTATATTGCG
GCATAGAACAACGCTTATCAATTGCCAAAGCCAAACGTGAGTTGGGCTACAATCCTTTGCCTGCGGTCGATGCCGTCCGT
AAGACTTTTCAGATTCTGGCTCGGCAAGCACCAAAGCAGGTTGCCTTGGGTCAAGTCTAG

Upstream 100 bases:

>100_bases
CTCTCTATTCTTAGGATATAGTTTATTCTATTATACTGGTCTCTTTTTTTATCTAAAACTAGATAAACTATTTGTCTAGT
TTATTATGAAAGGAATGGCA

Downstream 100 bases:

>100_bases
CTTGCGATCAATGTGGTGGCAGTTGTTTGGGCTGCCACCACATAGCGATTAATTAGCTATCGCGATCAACAATTGTGTTG
CGTAAGATGCCGATGCGTTC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSKRVLVTGANGHLGSVLAQMLVERGVDVRASVRNRSQIKPQLAYEQVYADLMDMDSLQQALVGVDTLYQVAAVFKHWSR
NPQREIIQPNVEGTRNILRAAAQAGVKRVVYVSSIAAVDKNNPQRQIPADETTWNQYTYGNPYYQSKIASEQLAWKLAKE
YGLEMMAGLPGTIIGDPNGRTTPSLGILELVLSNKMPLDINMDFNFVDVADVAEGLIAAERQGRAGERYILANDQSLPLR
RIFEIAQEFNPKIKVPMRVSKGITNVVAGMMELVANVTGREPMILRSQVGLYCGIEQRLSIAKAKRELGYNPLPAVDAVR
KTFQILARQAPKQVALGQV

Sequences:

>Translated_339_residues
MSKRVLVTGANGHLGSVLAQMLVERGVDVRASVRNRSQIKPQLAYEQVYADLMDMDSLQQALVGVDTLYQVAAVFKHWSR
NPQREIIQPNVEGTRNILRAAAQAGVKRVVYVSSIAAVDKNNPQRQIPADETTWNQYTYGNPYYQSKIASEQLAWKLAKE
YGLEMMAGLPGTIIGDPNGRTTPSLGILELVLSNKMPLDINMDFNFVDVADVAEGLIAAERQGRAGERYILANDQSLPLR
RIFEIAQEFNPKIKVPMRVSKGITNVVAGMMELVANVTGREPMILRSQVGLYCGIEQRLSIAKAKRELGYNPLPAVDAVR
KTFQILARQAPKQVALGQV
>Mature_338_residues
SKRVLVTGANGHLGSVLAQMLVERGVDVRASVRNRSQIKPQLAYEQVYADLMDMDSLQQALVGVDTLYQVAAVFKHWSRN
PQREIIQPNVEGTRNILRAAAQAGVKRVVYVSSIAAVDKNNPQRQIPADETTWNQYTYGNPYYQSKIASEQLAWKLAKEY
GLEMMAGLPGTIIGDPNGRTTPSLGILELVLSNKMPLDINMDFNFVDVADVAEGLIAAERQGRAGERYILANDQSLPLRR
IFEIAQEFNPKIKVPMRVSKGITNVVAGMMELVANVTGREPMILRSQVGLYCGIEQRLSIAKAKRELGYNPLPAVDAVRK
TFQILARQAPKQVALGQV

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=344, Percent_Identity=26.1627906976744, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI8393516, Length=344, Percent_Identity=26.1627906976744, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310132178, Length=331, Percent_Identity=25.6797583081571, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI310113012, Length=331, Percent_Identity=25.6797583081571, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI239745448, Length=331, Percent_Identity=25.6797583081571, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI87081792, Length=331, Percent_Identity=25.9818731117825, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71987463, Length=264, Percent_Identity=30.3030303030303, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320750, Length=303, Percent_Identity=25.4125412541254, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 37504; Mature: 37373

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRVLVTGANGHLGSVLAQMLVERGVDVRASVRNRSQIKPQLAYEQVYADLMDMDSLQQ
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ALVGVDTLYQVAAVFKHWSRNPQREIIQPNVEGTRNILRAAAQAGVKRVVYVSSIAAVDK
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNPQRQIPADETTWNQYTYGNPYYQSKIASEQLAWKLAKEYGLEMMAGLPGTIIGDPNGR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEECCCCC
TTPSLGILELVLSNKMPLDINMDFNFVDVADVAEGLIAAERQGRAGERYILANDQSLPLR
CCCCHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHH
RIFEIAQEFNPKIKVPMRVSKGITNVVAGMMELVANVTGREPMILRSQVGLYCGIEQRLS
HHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCHHCCHHHHHH
IAKAKRELGYNPLPAVDAVRKTFQILARQAPKQVALGQV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure 
SKRVLVTGANGHLGSVLAQMLVERGVDVRASVRNRSQIKPQLAYEQVYADLMDMDSLQQ
CCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ALVGVDTLYQVAAVFKHWSRNPQREIIQPNVEGTRNILRAAAQAGVKRVVYVSSIAAVDK
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNPQRQIPADETTWNQYTYGNPYYQSKIASEQLAWKLAKEYGLEMMAGLPGTIIGDPNGR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEECCCCC
TTPSLGILELVLSNKMPLDINMDFNFVDVADVAEGLIAAERQGRAGERYILANDQSLPLR
CCCCHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHH
RIFEIAQEFNPKIKVPMRVSKGITNVVAGMMELVANVTGREPMILRSQVGLYCGIEQRLS
HHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCHHCCHHHHHH
IAKAKRELGYNPLPAVDAVRKTFQILARQAPKQVALGQV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]